| تعداد نشریات | 284 |
| تعداد نشریات سازمان | 82 |
| تعداد شماره‌ها | 3,036 |
| تعداد مقالات | 33,971 |
| تعداد مشاهده مقاله | 45,650,497 |
| تعداد دریافت فایل اصل مقاله | 76,083,766 |
تعیین خصوصیات بیولوژیکی جدایه‌های ویروئید کاککسیای مرکبات در باغات نارنگی محلی (Citrus unshiu Marcovitch) در شرق مازندران | ||
| آفات و بیماری های گیاهی | ||
| مقاله 8، دوره 90، شماره 1 - شماره پیاپی 114، 1401، صفحه 87-97 اصل مقاله (625.23 K) | ||
| نوع مقاله: بیماری‌شناسی گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2022.359220.1444 | ||
| نویسندگان | ||
| پرسا تیموری1؛ سعید نصراله نژاد* 2؛ ,وجید علوی3؛ میثم تقی نسب4 | ||
| 11دانشجوی دکتری گروه گیاهپزشکی دانشگاه علوم کشاورزی و منابع طبیعی گرگان | ||
| 2دانشیار گروه گیاهپزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گرگان ایران | ||
| 3موسسه تحقیقات مرکبات ساری | ||
| 4دانش‌آموخته کارشناسی ارشد دانشگاه علوم کشاورزی و منابع طبیعی گرگان | ||
| چکیده | ||
| در سال‌های اخیر علائم مشکوک به بیماری ویروئیدی کاککسیای مرکبات(Citrus cachexia viroid) شامل آبله دوکی و وجود صمغ درحفرههای تنه در باغات مرکبات مازندران شیوع زیادی یافته است. این پژوهش به‌منظور تفکیک بیولوژیکی این ویروئید در باغات نارنگی محلی منطقه انجام شد. پس از نمونه برداری، بررسی با روش خالصسازی ستونکروماتوگرافی پودرسلولزCF-11 و RT-PCR با آغازگرهای اختصاصی انجام شد. برای تفکیک سویههای ویروئید ، روی 13 رقم از نهال‌های بذری ، پیوند پوستی انجام شد. نتایج نشان داد از مجموع 150 نمونه جمع‌آوری شده، 9 نمونه فقط به ویروئید کاککسیا آلوده بودند و سایر نمونهها دارای آلودگی مخلوط با ویروس‌های تریستزا، پسوروز و ویروئید اگزوکورتیس بود. نتایج بررسی های گلخانه ای نشان داد که بهترین گیاه محک برای تفکیک سویه های شدید و خفیف ویروئید کاککسیای مرکبات، رقم لیموی مکزیکی است. همچنین تفکیک سویههای شدید و خفیف، بر اساس علائم درختان نارنگی محلی امکان‌پذیر بوده و اندازه و شکل آبله های روی تنه در محل پیوند و صمغ زدگی زیر پوست، می‌تواند مبنای اولیه تفکیک آنها از یکدیگر قرار گیرد. | ||
| کلیدواژه‌ها | ||
| گیاه محک؛ لیموترش مکزیکی؛ ویروئید کاککسیای مرکبات؛ RT-PCR | ||
| عنوان مقاله [English] | ||
| Biological characteristics of Citrus cachexia viroid isolates in local tangerine orchards (Citrus unshiu Marcovitch) in East Mazandaran | ||
| نویسندگان [English] | ||
| Parsa Teymuri1؛ saeed nasrollanejad2؛ Vahid Alavi3؛ Mysam Taghinasab4 | ||
| 1Former PhD. student, Dept. of Plant protection, Gorgan University of Agricultural Science and Natural. Resources, Iran, | ||
| 2Associate Prof, Dept of Plant Protection, Gorgan University of Agricultural Sciences and Natural Resources | ||
| 3Department of citrus institute of Sari | ||
| 4Former PhD. student, Dept. of Plant protection, Gorgan University of Agricultural Science and Natural. Resources, | ||
| چکیده [English] | ||
| In recent years symptoms of viroid diseases, include stem pitting and gumming have highly incidenced in citrus orchards of Mazandaran. This study was conducted for determining the biological characteristics of Citrus cachexia viroid strains in local tangerine orchards. 150 samples were collected from three important infected districts. Purification was carried out in CF-11 column. Presence of viroid was confirmed with RT-PCR using specific primers. For differentiation of Cachexia viroid isolates, 13 types of citrus were bark grafted on rootstock and symptoms were observed. RT-PCR data showed that 6% of samples were infected only with cachexia, while other samples showed mix infection with exocortis, tristeza and psorosis. According to the assay, the best indicator plant is the Mexican lime (Citrus aurantifolia. Swing.), which could express the typical symptoms of mild to severe infection. Also it is possible to separate severe strains from mild strains based on the symptoms on the local tangerine trees and gumming on the branches can be the primary basis for differentiation. | ||
| کلیدواژه‌ها [English] | ||
| Citrus cachexia viroid, indicator plant, Mexican lime, RT-PCR, stem pitting | ||
|
سایر فایل های مرتبط با مقاله
|
||
| مراجع | ||
|
ACHACHI, A., E. AIT BARKA and M. IBRIZ, 2014. Recent advances in Citrus psorosis virus. VirusDis. 25(3), 261–276 AHMADI, K., H.R. EBADZADE., HATAMI,F., SH. MOHAMADNIA, R. TAGHANI and M.A. KALANTARI, 2021. Agricultural statistic (horticultural products), information and communication technology center of the ministry of jihad agriculture, pp110-121 (in Farsi with English summary) ALAVI, V., B. KHATABI and G. HOSSEINI SALEKDEH, 2005. Comparison of biologically distinct isolates of Citrus tristeza virus from Iran using major coat protein sequences. Australasian Plant Pathology, No. 34: 577-582. (in Farsi with English summary) ALAVI, S.M., A. AHOUNMANESH, H.A. RAHIMIAN, 2007. Nucleotide Sequence Of Hop Stunt Viroid cDNA From Cachexia infected Citrus Plants In Mazandaran Province. Iranian Journal of Plant Pathology, No. 43:1(69): 49-64. ALAVI, S.V., P. TEYMURI, 2017. Detection of citrus viroids by CF-11 nucleic acid extraction method. Applied Entomology and Phytopathology, No.85 (1): 77-86 ALCANTARA-MENDOZA, S.; S. ALCANTARA-MENDOZA; O GARCÍA-RUBIO; V.H.; CAMBRÓN-SAN-DOVAL, D. CAMBRÓN-SAN-DOVAL, C. CAMBRÓN-SAN-DOVAL, 2017. Characterization of Citrus Exocortis viroid in different conditions of indexing. Mexican Journal pf Phytopathology, No. 35: 284-303. ALMEYDA, F., L., MARCIO RODRIGUES, C. COELHO, 2019. The still underestimated Problem of Fungal diseases worldwide. Frontiers in Microbiology,No 34: 24-31 ALMAYDA-LEON, I. H., M.A. ROCH-PENA, IRACHETACARDENAS, F. ORONA-STERO and C.J. KHALKE, 2007. A simple method for the multiple detection of citrus viroids. Agrociencia. No. 41: 87-93 APARI-ISAIA, T., A. KYRIAKOU, L. PAPAYIANNIS, D. TSALTAS, S. GREGORIOU and I. PSALTIS, 2008. Rapid in vitro microindexing of viroids in citrus. Plant Pathology., No. 57, 348-353 ALVARADO-GOMEZ, O.G., M.A. ROCH-PENA, S. SILVA-VARA, J.P. MARTINEZ-SORIANO and R.F LEE, 2000. Citrus exocortis and citrus cachexia viroids in commercial groves of Tahiti lime in Mexico .15th. International Organization of Citrus Virologist Confrance USA: 289-293. BANI-HASHEMIAN, S.M., G. PENSABENE-BELLAVIA, N. DURAN-VILA and P. SERRA, 2015. Phloem restriction of viroids in three citrus hosts is overcome by grafting with Etrog citron: Potential involvement of a translocateable factor. Journal of General Virology, No. 96, 2405–2410. BERNARD, L. and DURAN-VILA, 2006. A novel RT-PCR approach for detection and characterization of citrus viroids. Molecular and Cellular Probes, No. 20(2): 105-13. EIRAS, M., S.R SILVA, E.S., STUCHI, S.A., CARVALHO and R.M., GARCÊZ, 2013. Identification and characterization of viroids in “Navelina ISA 315” sweet orange. Tropical Plant Pathology, No.38(1), 58-62. FALAKI, F.S.V. and ALAVI F., RAKHSHANDEROO, 2012. Citrus Psorosis Virus,causal agent of ring pattern disorder in Thomson Navel trees in east of Mazandaran. Applied Entomology and Phytopathology, No.80(2):161-172(in Farsi with English summary) HATAYA, T., T. TSUSHIMA and T. SANO, 2017. Hop stunt viroid. In: Hadidi, A., Randles, J., Flores, R. and Palukaitis, P. (Eds.), Viroids and Satellites. Cambridge: Academic Press-Elsevier, pp. 199–210. HULL, R. 2002. “Mathews plant virology” (4th edition) .Academic press, SanDiego. 1001pp. HUANG, Z., P.A. RUNDELL, X. GUAN and C.A. POWELL, 2004. Detection and isolate differentiation of Citrus tristeza virus in infected field trees based on reverse transcription–polymerase chain reaction. Plant Diseases. 88:625-629. LOCONSOLEA, G., B. NUKET ÖNELGEK, RAYMOND, C. YOKOMID., RAIED ABOU KUBAA, A. VITO SAVINO and M. SAPONARI, 2013. Molecular.Rapid differentiation of Citrus Hop stunt viroid variants by real-time RT-PCR and high resolution melting analysis. Molecular and Cellular Probes, No. 27(5) MALFITANO, M.; M BARONE,.; N.; DURAN-VILA, D. ALIOTO, 2005. Indexing of viroids in citrus orchards of Campania, Southern Italy. J. Plant Pathol., 87, 115-121- MARQUEZ‐MOLINS, j., G. GOMEZ and V. PALLAS, 2021. Hop stunt viroid: A polyphagous pathogenic RNA that has shed light on viroid–host interactions. Molecular Plant Pathology. No. 22(2): 153–162. MORRIS, T.J. and J.A. Dodds, 1979. Isolation and analysis of double stranded RNA from virus-infected plant and fungal tissue .Phytopathology. No. 69: 854-858. MURCIA, N., S.M., BANI HASHEMIAN, P., SERRA, J.A PINA.and N.DURAN-VILA, 2015. Citrus viroids: Symptom expression and performance of Washington navel sweet orange trees grafted on Carrizo citrange. Plant Disease. 99:125-136. NAJAR .A, I. HAMDI , K., BEN MAHMOUD, 2018. Citrus viroids: Characterization, prevalence, distribution and struggle methods. Journal of new sciences, Agriculture and Biotechnology, No. 50(12), 3129-3137 PALACIO-BIELSA, A., J. ROMERO-DURB´AN, and N. DURAN-VILA, 2004. Characterization of citrus HSVd isolates. Archive of Virology, No. 149: 537–552. PEREZ, R.; E. CORREA, N. DEL VALLE and O. OTERO, 2000. Reduced Indexing Time for Cachexia and Exocortis Diseases in Citrus. In Proceedings of the Fourteenth International Organization of Citrus Virologists Conference; California.386-387. ROISTACHER. C.N., 1991. Graft-Transmissible Diseases of Citrus . FAO: Rom .286pp. FLORES, R., HERNANDES, C., MARTINEZDE-ALBA, A., DAROS, A. and DISERIO, F. 2005. Viroid and viroid-host interaction. Annual Review of Phytopathology, No. 43: 117-139. ROISTACHER, C.N., 2007. Diagnosis and management of virus and virus like diseases of Citrus. In Diseases of Fruits and Vegetables, No.1:109-189. SANO TERUO, 2021. Progress in 50 years of viroid research—Molecular structure, pathogenicity, and host adaptation. Proceeding pf the Japan Academi. Series B, No. 97(7): 371-401. SEMANCIK, J.S. and C.N. ROISTACHER, 1988. A new viroid is the causal agent of the citrus Cachexia disease. Virology, No. 10: 125-135. SERRA, P., S. GAGO and N. DURAN-VILA, 2008. A single nucleotide change in Hop Stunt Viroid modulates citrus cachexia symptoms. Virus Research, 138(1-2), 130-134 STACKHOUSE, J.E., L. OLIVERJ, WILLIAMS-WOODWARD and M.E. ALI, 2021. First Report of Hop Stunt Viroid Infecting Citrus Trees in Georgia, USA. Plant Disease, No. 105:515. SU, X., S. FU, Y. QIAN, Y. XU and X. ZHOU, 2015. Identification of Hop Stunt Viroid infecting Citrus limon in China using small RNAs deep sequenc-ing approach. Journal of Virology, NO. 12: 1–5. VAMENANI, R., H. RAHIMIAN, S.M. ALAVI, A. PAKDIN PARIZI and T. MIRZA RAZZAZ, 2019. Genetic diversity of Hop Stunt Viroid from symptomatic and a-symptomatic citrus trees in Iran. Journal of Phytopathology, No. 167, 484–489 VELAZQUEZ, K., M. SOTO, R. PEREZ, J. PEREZ, M.D. RODRÍGUEZ and N. DURAN-VILA, 2002. Biological and molecular characterization of two isolates of Citrus viroids recovered from Cuban plantations. International Organization of Citrus Virologists Conference Proceedings, USA. No. 15(15), 258-263. VENKATARAMAN, S., U. BADAR, E. SHOEB, G. HASHIM, M. ABOUHAIDAR and H. HEFFERON, 2021.An Inside Look into Biological Miniatures: Molecular Mechanisms of Viroids. Journal of Molecular Science, No. 22(6): 2795.. VERDEJO-LUCAS, S., M.V. MCKENRY, 2004. Management of the Citrus Nematode, Tylenchulus semipenetrans .Journal of Nematology, No. 36(4): 424-32.
| ||
|
آمار تعداد مشاهده مقاله: 483 تعداد دریافت فایل اصل مقاله: 375 |
||
