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Araghi, A, Taghizadeh, M, Hosseini Doust, S. R, Paradise, A, Azimi Dezfouli, S. M. (1402). Evaluation of Immunogenicity of Clostridium perfringens Type B Toxoid and Inactivated FMD (O) Virus with Adjuvant (ISA70-MF59). سامانه مدیریت نشریات علمی, 78(3), 907-913. doi: 10.22092/ari.2022.360215.2565
A Araghi; M Taghizadeh; S. R Hosseini Doust; A Paradise; S. M Azimi Dezfouli. "Evaluation of Immunogenicity of Clostridium perfringens Type B Toxoid and Inactivated FMD (O) Virus with Adjuvant (ISA70-MF59)". سامانه مدیریت نشریات علمی, 78, 3, 1402, 907-913. doi: 10.22092/ari.2022.360215.2565
Araghi, A, Taghizadeh, M, Hosseini Doust, S. R, Paradise, A, Azimi Dezfouli, S. M. (1402). 'Evaluation of Immunogenicity of Clostridium perfringens Type B Toxoid and Inactivated FMD (O) Virus with Adjuvant (ISA70-MF59)', سامانه مدیریت نشریات علمی, 78(3), pp. 907-913. doi: 10.22092/ari.2022.360215.2565
Araghi, A, Taghizadeh, M, Hosseini Doust, S. R, Paradise, A, Azimi Dezfouli, S. M. Evaluation of Immunogenicity of Clostridium perfringens Type B Toxoid and Inactivated FMD (O) Virus with Adjuvant (ISA70-MF59). سامانه مدیریت نشریات علمی, 1402; 78(3): 907-913. doi: 10.22092/ari.2022.360215.2565

Evaluation of Immunogenicity of Clostridium perfringens Type B Toxoid and Inactivated FMD (O) Virus with Adjuvant (ISA70-MF59)

Archives of Razi Institute
مقاله 16، دوره 78، شماره 3، مرداد و شهریور 2023، صفحه 907-913    اصل مقاله (587.5 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.360215.2565
نویسندگان
A Araghi1؛ M Taghizadeh2؛ S. R Hosseini Doust* 3؛ A Paradise4؛ S. M Azimi Dezfouli5
1Department of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2Department of Research and Development, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
3Department of Microbiology, Faculty of Advanced Sciences, Islamic Azad University Tehran Medical Sciences, Tehran, Iran
4Department of Anaerobic Vaccine Research and Production, Specialized Clostridia Research Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
5Department of Foot and Mouth Vaccine Production, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
چکیده
Foot and mouth disease (FMD) and enterotoxemia are important diseases of hoofed animals. Vaccination against livestock pathogens, especially these two diseases, plays a key role in the prevention and control of these diseases. The use of combined vaccines with the aim of creating a better immune response and producing cheaper vaccines is a great contribution to Vaccine industry. This research aimed to compare the immunogenicity of FMD (O) and Clostridium perfringens type B toxoid along with adjuvant (MF59) and Montanide (ISA70) to create the best immunogenicity. To investigate the immune responses of vaccines, it was injected into an animal model, and the antibody titer was measured by enzyme-linked immunosorbent assay (ELISA) test and VN antibody titer. The results showed that the formulation with MF59 adjuvant brought more stable immunogenicity against FMD and Clostridium perfringens type B, and the length of the immunogenicity period also increased significantly. Therefore, the combined vaccine (Clostridium perfringens + FMD) could play a major role invaccine industry as an alternative vaccine against Clostridium perfringens and FMD in livestock.
کلیدواژه‌ها
Clostridium perfringens؛ Combination vaccine؛ FMD؛ ISA70؛ MF59
عنوان مقاله [English]
ارزیابی ایمنی زایی توکسوئید Clostridium perfringens type (B) و ویروس غیر فعال FMD(O) همراه با ادجوانت (ISA70-MF59)
چکیده [English]
بیماری تب برفکی (FMD) و (آنتروتوکسمی) از بیماری‌های مهم حیوانات سم دار می باشند. واکسیناسیون علیه پاتوژنهای دامی به ویژه این دو بیماری نقش مهمی در پیشگیری و کنترل دارد. استفاده از واکسن های ترکیبی(combination vaccine) با هدف ایجاد پاسخ ایمنی بهتر و تولید واکسن موثر و ارزانتر کمک بسیار مهمی به این صنعت است. این پروژه با هدف مقایسه ایمنی زایی ویروس FMD(O) و توکسوئید Clostridium perfringens type (B) همراه با ادجوانت ( MF59) وMontanide(ISA70) انجام گردید، تا بتواند ایمنی زایی مطلوب تری ایجاد نماید. لذا جهت بررسی پاسخ‌های ایمنی واکسن ها، به مدل حیوانی تزریق شدند و تیتر آنتی بادی با تست الایزا و VN antibody titer سنجیده شد. نتایج نشان داد که فرمولاسیون با ادجوانت MF59 ایمنی زایی بیشتر و پایدارتری بر علیه بیماری تب برفکی و Clostridium perfringens type (B) ایجاد نموده، و طول دوره ایمنی زایی نیز افزایش معنا داری می یابد. بنابراین، واکسن ترکیبی Clostridium perfringens - FMD می تواند در مقیاس صنعتی به عنوان یک واکسن جایگزین علیه Clostridium perfringens و FMD در دامها ایفای نقش کند.
کلیدواژه‌ها [English]
تب برفکی, کلستریدیوم پرفرینجنس, واکسن ترکیبی, MF59, ISA70
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