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Al Talebi, Z, Karhib, M. M, Taki, M. M, Salaam Abood, E, Mubarak, H. A, Sahar Ahmed Ibrahim, C. (1402). Chemical Analysis, Antioxidant, and Antibacterial Potential of Aqueous Extract of Broccoli (Brassica oleracea) Using GC-ms. سامانه مدیریت نشریات علمی, 78(2), 593-599. doi: 10.22092/ari.2022.359860.2488
Z Al Talebi; M. M Karhib; M. M Taki; E Salaam Abood; H. A Mubarak; C Sahar Ahmed Ibrahim. "Chemical Analysis, Antioxidant, and Antibacterial Potential of Aqueous Extract of Broccoli (Brassica oleracea) Using GC-ms". سامانه مدیریت نشریات علمی, 78, 2, 1402, 593-599. doi: 10.22092/ari.2022.359860.2488
Al Talebi, Z, Karhib, M. M, Taki, M. M, Salaam Abood, E, Mubarak, H. A, Sahar Ahmed Ibrahim, C. (1402). 'Chemical Analysis, Antioxidant, and Antibacterial Potential of Aqueous Extract of Broccoli (Brassica oleracea) Using GC-ms', سامانه مدیریت نشریات علمی, 78(2), pp. 593-599. doi: 10.22092/ari.2022.359860.2488
Al Talebi, Z, Karhib, M. M, Taki, M. M, Salaam Abood, E, Mubarak, H. A, Sahar Ahmed Ibrahim, C. Chemical Analysis, Antioxidant, and Antibacterial Potential of Aqueous Extract of Broccoli (Brassica oleracea) Using GC-ms. سامانه مدیریت نشریات علمی, 1402; 78(2): 593-599. doi: 10.22092/ari.2022.359860.2488

Chemical Analysis, Antioxidant, and Antibacterial Potential of Aqueous Extract of Broccoli (Brassica oleracea) Using GC-ms

Archives of Razi Institute
مقاله 12، دوره 78، شماره 2، خرداد و تیر 2023، صفحه 593-599    اصل مقاله (625.93 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359860.2488
نویسندگان
Z Al Talebi* 1؛ M. M Karhib2؛ M. M Taki3؛ E Salaam Abood4؛ H. A Mubarak5؛ C Sahar Ahmed Ibrahim6
1Chemistry Department, College of Science, University of Babylon, Babylon, Iraq
2Department of Medical Laboratory Techniques, Al-Mustaqbal University College, 51001 Hillah, Babylon, Iraq
3Nanotechnology and Advanced Materials Research Centre, University of Technology, Baghdad, Iraq
4Medical Physics Department, Hilla University College, Babylon, Iraq
5Department of Chemical Engineering, College of Engineering, University of Babylon, Babylon, Iraq
6Babil Teaching Hospital For Maternity and Children, Babylon, Iraq
چکیده
Antioxidant and antibacterial chemicals are key sources in medicinal plants. Alkaloids, phenolics, steroids, terpenes, flavonoids, terpenes, and volatile oils are a few of these plants' secondary metabolites. Phytochemicals, particularly the secondary metabolites produced by plants, are important for human nutrition, well-being, illness prevention, and antibacterial properties. This study aimed to ascertain the aqueous broccoli extract's chemical makeup. The phytochemical molecule that the GC-MS technique identified. The DPPH assay, which is appropriate for regular plant material screening, was performed to assess the antioxidant capacities of broccoli extract (in vitro). Subsequently looks at how well they perform against different Gram-positive and Gram-negative harmful microorganisms. GC-MS analysis of Broccoli extract revealed the existence of the9-Octadecenamide, [C18H35O], Hexadecane [c16h34] and 2, 2, 2-trifluoroethyl 2-methyltetrahydro-5-oxo-3- furancarboxylate [C23H33NO6]. There were significant changes in the extract's ascorbic acid-free radical scavenging activity at 200, 100, and 25 g/ml (P 0.05), and the activity was dose-dependent. The effectiveness of aqueous broccoli extract as a powerful, broad-spectrum antibacterial agent against tested bacteria is demonstrated by an increase in the diameter of the inhibition zone, which increases in direct proportion to the concentration of the extract and even surpasses the activity of some antibiotics. An appropriate concentration of aqueous broccoli extract strongly inhibits microbial and antioxidant growth, especially when treating external infections without any danger against resistant bacterial isolates; it is strongly advised to use aqueous broccoli extract as a cost-effective alternative antibacterial and antioxidant agent.
کلیدواژه‌ها
Aqueous Broccoli Extract؛ GC-MS؛ Antioxidant؛ Antibacterial
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