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Soltani, M, Nejati, A, Marashi, S. M, Nili, F, Yaseri, M, Mokhtari Azad, T. (1402). Diverse Expression Patterns of EBV Oncogenes (LMP2A, EBV-Encoded microRNA, and EBV-encoded dUTPase) in EBV Associated Gastric Carcinoma and their Association with Viral Loads. سامانه مدیریت نشریات علمی, 78(2), 643-649. doi: 10.22092/ari.2022.359408.2415
M Soltani; A Nejati; S. M Marashi; F Nili; M Yaseri; T Mokhtari Azad. "Diverse Expression Patterns of EBV Oncogenes (LMP2A, EBV-Encoded microRNA, and EBV-encoded dUTPase) in EBV Associated Gastric Carcinoma and their Association with Viral Loads". سامانه مدیریت نشریات علمی, 78, 2, 1402, 643-649. doi: 10.22092/ari.2022.359408.2415
Soltani, M, Nejati, A, Marashi, S. M, Nili, F, Yaseri, M, Mokhtari Azad, T. (1402). 'Diverse Expression Patterns of EBV Oncogenes (LMP2A, EBV-Encoded microRNA, and EBV-encoded dUTPase) in EBV Associated Gastric Carcinoma and their Association with Viral Loads', سامانه مدیریت نشریات علمی, 78(2), pp. 643-649. doi: 10.22092/ari.2022.359408.2415
Soltani, M, Nejati, A, Marashi, S. M, Nili, F, Yaseri, M, Mokhtari Azad, T. Diverse Expression Patterns of EBV Oncogenes (LMP2A, EBV-Encoded microRNA, and EBV-encoded dUTPase) in EBV Associated Gastric Carcinoma and their Association with Viral Loads. سامانه مدیریت نشریات علمی, 1402; 78(2): 643-649. doi: 10.22092/ari.2022.359408.2415

Diverse Expression Patterns of EBV Oncogenes (LMP2A, EBV-Encoded microRNA, and EBV-encoded dUTPase) in EBV Associated Gastric Carcinoma and their Association with Viral Loads

Archives of Razi Institute
مقاله 18، دوره 78، شماره 2، خرداد و تیر 2023، صفحه 643-649    اصل مقاله (537.75 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359408.2415
نویسندگان
M Soltani1؛ A Nejati1؛ S. M Marashi1؛ F Nili2؛ M Yaseri3؛ T Mokhtari Azad* 1
1Virology Department, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
2Department of Pathology, Imam Khomeini hospital complex, Tehran University of Medical Sciences, Tehran, Iran
3Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
چکیده
The chromogenic in situ hybridization (CISH) test is the gold standard for detecting Epstein-Barr virus (EBV)-associated gastric carcinoma (GC). Real-time (RT) PCR method is also a sensitive test that can detect the viral load in samples. As such, three EBV oncogenes were investigated in this study. RNA extraction and cDNA synthesis were performed on GC tissues of nine patients, who were previously confirmed to have EBVGC subtype. In addition, 44 patients that had positive RT-PCR but negative CISH results were also included as the control group. TaqMan RT-PCR analysis was performed to determine the expression of EBV-encoded microRNAs, and the expression of EBV-encoded dUTPase, as well as LMP2A, was analyzed by SYBR Green RT-PCR. EBV-encoded microRNAs and LMP2A were identified in 2 out of 9 (22%) EBVGC subtypes. In addition, EBV-encoded dUTPase was detected in 4 out of 9 (44.5%) EBVGC subtypes. EBV-encoded dUTPase was also expressed in a sample of the control group. The expression of LMP2A, EBV-encoded microRNAs, and EBV-encoded dUTPase viral oncogenes in patients with high EBV viral loads indicates that these expressions correlate with viral loads. Our findings indicate that the EBV-encoded dUTPase gene may have a role in EBVGC patients’ non-response to treatment and might be considered a Biomarker-targeted therapy.
کلیدواژه‌ها
DUTPase؛ Epstein-Barr virus؛ Gastric carcinoma؛ LMP2A؛ MicroRNA
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مراجع
  1. Farrell PJ. Epstein–Barr virus and cancer. Annu Rev Pathol: Mech Dis. 2019;14:29-53.
  2. Zur Hausen H. The search for infectious causes of human cancers: where and why. Virology. 2009;392(1):1-10.
  3. Network CGAR. Comprehensive molecular characterization of gastric adenocarcinoma. Nature. 2014;513(7517):202.
  4. Luo B, Wang Y, Wang X-F, Liang H, Yan L-P, Huang B-H, et al. Expression of Epstein-Barr virus genes in EBV-associated gastric carcinomas. World J Gastroenterol. 2005;11(5):629.
  5. Murphy G, Pfeiffer R, Camargo MC, Rabkin CS. Meta-analysis shows that prevalence of Epstein–Barr virus-positive gastric cancer differs based on sex and anatomic location. Gastroenterology. 2009;137(3):824-33.
  6. Glaser R, Litsky ML, Padgett DA, Baiocchi RA, Yang EV, Chen M, et al. EBV-encoded dUTPase induces immune dysregulation: Implications for the pathophysiology of EBV-associated disease. Virology. 2006;346(1):205-18.
  7. Sommer P, Kremmer E, Bier S, König S, Zalud P, Zeppezauer M, et al. Cloning and expression of the Epstein—Barr virus-encoded dUTPase: patients with acute, reactivated or chronic virus infection develop antibodies against the enzyme. J Gen Virol. 1996;77(11):2795-805.
  1. Williams MV, Cox B, Ariza ME. Herpesviruses dUTPases: a new family of pathogen-associated molecular pattern (PAMP) proteins with implications for human disease. Pathogens. 2017;6(1):2.
  2. Pal AD, Basak NP, Banerjee AS, Banerjee S. Epstein–Barr virus latent membrane protein-2A alters mitochondrial dynamics promoting cellular migration mediated by Notch signaling pathway. Carcinogenesis. 2014;35(7):1592-601.
  3. Hino R, Uozaki H, Inoue Y, Shintani Y, Ushiku T, Sakatani T, et al. Survival advantage of EBV-associated gastric carcinoma: survivin up-regulation by viral latent membrane protein 2A. Cancer Res. 2008;68(5):1427-35.
  4. Portis T, Longnecker R. Epstein–Barr virus (EBV) LMP2A mediates B-lymphocyte survival through constitutive activation of the Ras/PI3K/Akt pathway. Oncogene. 2004;23(53):8619-28.
  5. Navari M, Etebari M, Ibrahimi M, Leoncini L, Piccaluga PP. Pathobiologic roles of epstein–barr virus-encoded microRNAs in human lymphomas. Int J Mol Sci. 2018;19(4):1168.
  6. Allum W, Meyer H, Garofalo A, Schuhmacher J, Demanzoni G, Degiuli M, et al. Gastric cancer in Europe: European union network of excellence (EUNE) for gastric cancer steering group. 2008.
  7. Chen X-Z, Chen H, Castro FA, Hu J-K, Brenner H. Epstein–Barr virus infection and gastric cancer: a systematic review. Medicine. 2015;94(20).
  8. Vértessy BG, Tóth J. Keeping uracil out of DNA: physiological role, structure and catalytic mechanism of dUTPases. Acc Chem Res. 2009;42(1):97-106.
  9. Fleischmann J, Kremmer E, Müller S, Sommer P, Kirchner T, Niedobitek G, et al. Expression of deoxyuridine triphosphatase (dUTPase) in colorectal tumours. Int J Cancer. 1999;84(6):614-7.
  10. Kawahara A, Akagi Y, Hattori S, Mizobe T, Shirouzu K, Ono M, et al. Higher expression of deoxyuridine triphosphatase (dUTPase) may predict the metastasis potential of colorectal cancer. J Clin Pathol. 2009;62(4):364-9.
  11. Wilson PM, Fazzone W, LaBonte MJ, Lenz H-J, Ladner RD. Regulation of human dUTPase gene expression and p53-mediated transcriptional repression in response to oxaliplatin-induced DNA damage. Nucleic Acids Res. 2009;37(1):78-95.
  1. Yang J, Liu Z, Zeng B, Hu G, Gan R. Epstein–Barr virus-associated gastric cancer: A distinct subtype. Cancer Lett. 2020;495:191-9.
  2. Zheng X, Wang J, Wei L, Peng Q, Gao Y, Fu Y, et al. Epstein-Barr virus microRNA miR-BART5-3p inhibits p53 expression. J Virol. 2018;92(23).
  3. De Re V, Caggiari L, De Zorzi M, Fanotto V, Miolo G, Puglisi F, et al. Epstein-Barr virus BART microRNAs in EBV-associated Hodgkin lymphoma and gastric cancer. Infect Agents Cancer. 2020;15(1):1-9.
  4. Yang Y-C, Liem A, Lambert PF, Sugden B. Dissecting the regulation of EBV's BART miRNAs in carcinomas. Virology. 2017;505:148-54.
  1. Hooykaas MJ, Kruse E, Wiertz EJ, Lebbink RJ. Comprehensive profiling of functional Epstein-Barr virus miRNA expression in human cell lines. BMC Genom. 2016;17(1):1-13.
  2. Shinozaki-Ushiku A, Kunita A, Isogai M, Hibiya T, Ushiku T, Takada K, et al. Profiling of virus-encoded microRNAs in Epstein-Barr virus-associated gastric carcinoma and their roles in gastric carcinogenesis. J Virol. 2015;89(10):5581-91.
  3. Tsai CY, Liu YY, Liu KH, Hsu JT, Chen TC, Chiu CT, et al. Comprehensive profiling of virus microRNAs of Epstein–Barr virus‐associated gastric carcinoma: highlighting the interactions of ebv‐Bart9 and host tumor cells. J Gastroenterol Hepatol. 2017;32(1):82-91.
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