| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شماره‌ها | 3,262 |
| تعداد مقالات | 36,060 |
| تعداد مشاهده مقاله | 55,194,532 |
| تعداد دریافت فایل اصل مقاله | 94,191,201 |
تجزیه ژنتیکی برخی صفات کمّی آویشن دنایی (Thymus daenensis Celak) در شرایط تنش شوری از طریق آزمون پلی‌کراس | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 5، دوره 38، شماره 4 - شماره پیاپی 114، دی 1401، صفحه 564-580 اصل مقاله (684.23 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2022.359229.3200 | ||
| نویسندگان | ||
| امیرعلی شایان1؛ مجید شکرپور* 2؛ وحیده ناظری3؛ مصباح بابالار3؛ علی اشرف مهرابی4 | ||
| 1دانشجوی دکترا، گروه علوم باغبانی پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران | ||
| 2دانشیار، گروه علوم باغبانی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران | ||
| 3استاد، گروه علوم باغبانی پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران | ||
| 4دانشیار، بخش زیست‌فناوری مؤسسه تحقیقات جنگلها و مراتع کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| چکیده | ||
| توسعه کشت آویشن دنایی (Thymus daenensis Celak) سازگار به شرایط محیطی خاص از طریق اصلاح جمعیت، نیازمند ایجاد یک خزانه ژنتیکی غنی از ژنوتیپ‌های برتر و بهره‌برداری از این تنوع برای شناسایی ژنوتیپ‌های با ترکیب‌پذیری بالا می‌باشد. در این پژوهش، به‌منظور مطالعه تنوع ژنتیکی و میزان ترکیب‌پذیری اکوتیپ‌های مختلف آویشن دنایی در شرایط شوری، آزمایشی به‌صورت فاکتوریل در قالب طرح بلوک‌های کامل تصادفی با سه تکرار در شرایط گلخانه‌ای اجرا شد. فاکتورها شامل تنش شوری (0 و 90 میلی‌مولار نمک NaCl) و اکوتیپ (12 خانواده ناتنی حاصل از تلاقی پلی‌کراس بین 12 اکوتیپ آویشن دنایی) بودند. شوری سبب کاهش معنی‌دار همه صفات (بجز مقادیر پرولین و کاروتنوئید) نسبت به شاهد گردید. اکوتیپ‌ها از نظر طول و عرض برگ، طول شاخه‌های جانبی، وزن خشک اندام‌های هوایی، میزان اسانس و پرولین اختلاف معنی‌دار داشتند. درصد اسانس و عرض برگ دارای بیشترین ضریب تنوع فنوتیپی و ژنوتیپی بودند. وراثت‌پذیری عمومی صفات مورد مطالعه از 4.61% (طول برگ) تا 81.07% (عرض برگ) متغیر بود. بیشترین میزان وراثت‌پذیری به عرض برگ، میزان اسانس و وزن خشک اندام‌های هوایی اختصاص داشت. با توجه به میزان بالای ضریب تنوع ژنوتیپی و فنوتیپی، وراثت‌پذیری و پیشرفت ژنتیکی میزان اسانس و وزن خشک اندام‌های هوایی، از این صفات می‌توان به‌عنوان مهم‌ترین معیارهای انتخاب والدین در برنامه‌های اصلاحی آویشن دنایی نام برد. نتایج نشان داد که اکوتیپ‌های ملایر2، زاغه، اراک و ایلام دارای ترکیب‌پذیری بالایی از نظر صفت میزان اسانس بودند. با توجه به تنوع ژنتیکی کافی مشاهده شده میان اکوتیپ‌های مطالعه شده و ترکیب‌پذیری مناسب آنها، راهبرد اصلاح جمعیت با تولید ارقام ساختگی (ترکیبی) را می‌توان برای به‌نژادی این گونه در شرایط نرمال (بدون تنش) و شوری توصیه نمود. | ||
| کلیدواژه‌ها | ||
| آویشن دنایی (Thymus daenensis Celak)؛ تنش شوری؛ ترکیب‌پذیری عمومی؛ خانواده ناتنی؛ رقم سنتتیک | ||
| عنوان مقاله [English] | ||
| Genetic analysis of some quantitative traits in Thymus daenensis Celak under salinity stress using Polycross test | ||
| نویسندگان [English] | ||
| A. Shayan1؛ M. Shokrpour2؛ V. Nazeri3؛ M. Babalar3؛ A. Mehrabi4 | ||
| 1Ph.D. student, Department of Horticulture, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran | ||
| 2, Department of Horticulture, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran | ||
| 3Department of Horticulture, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran | ||
| 4Plant Molecular Breeding, Department of Plant Biotechnology, Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran | ||
| چکیده [English] | ||
| Cultivation development of adaptable Thymus daenensis Celak to specific environmental conditions through population improvement requires the creation of a genetic pool rich of superior genotypes and exploitation of this diversity to identify genotypes with high combinability. In this research, to study the genetic diversity and combinability of different T. daenensis ecotypes under salinity, a factorial greenhouse experiment was conducted in a randomized complete block design with three replications. The factors included salinity stress (0 and 90 mM NaCl) and ecotype (12 half-sib families derived from a polycross between 12 T. daenensis ecotypes). Salinity decreased all the measured traits (except for proline and carotenoids content) significantly compared to the control. The ecotypes differed significantly from each other in terms of length and leaf width, length of lateral branches, aerial parts dry weight, essential oil content, and proline content. Essential oil content and leaf width obtained the highest phenotypic and genotypic coefficient of variation. General heritability of the studied traits varied from 4.61% (leaf length) to 81.07% (leaf width). The highest general heritability was assigned to leaf width, essential oil content, and aerial parts dry weight. Regarding the higher genotypic and phenotypic coefficient of variation, heritability, and genetic advance of the traits including essential oil content and aerial parts dry weight, these traits could be considered as the most important criteria for the parents selection in the breeding programs of T. daenensis. The results showed that the ecotypes Malayer2, Zagheh, Arak, and Ilam had high combinability in terms of essential oil content. Considering the sufficient genetic diversity observed between the studied ecotypes along with their adequate combinability, it could be recommended to breed T. daenensis through the strategy of improving the population by producing synthetic cultivars for normal (non-stress) and salinity conditions. | ||
| کلیدواژه‌ها [English] | ||
| Thymus daenensis Celak, salinity, general combining analysis, half-sib family, synthetic cultivar | ||
| مراجع | ||
|
- Acosta-Motos, J.R., Hernández, J.A., Álvarez, S., Barba-Espín, G. and Sánchez-Blanco, M.J., 2017. Long-term resistance mechanisms and irrigation critical threshold showed by Eugenia myrtifolia plants in response to saline reclaimed water and relief capacity. Plant Physiology and Biochemistry, 111: 244-256. - Aastveit, A.H. and Aastveit, K., 1990. Theory and application of open-pollination and polycross in forage grass breeding. Theoretical and Applied Genetics, 79: 618-624. - Ashraf, M., 1994. Breeding for salinity tolerance in plants. Critical Reviews in Plant Sciences. 13: 17-42. - Aziz, E., Al-Amier, H. and Craker, L., 2008. Influence of salt stress on growth and essential oil production in peppermint, pennyroyal, and apple mint. Journal of Herbs, Spices, & Medicinal Plants, 14: 77-87. - Babaie, K., Jabbari, R., Amini Dehaghi, M. and Modares Sanav, A., 2010. Effect of saline stress on morphological, physiologic and chemical characteristics of Thyme (Thymus vulgaris L.). Agronomy Journal (Pajouhesh & Sazandegi), 86: 71-79. - Babalar, M., Askari, M., Naderi, R., Kafi, M., Dani, H., Ahmadi, A., Zolfaghari, M., Salehi, F., Godarzi, M., Panahi, H., Shalahi, GH., Rezaii, H. and Tadbiri, M., 2010. Zeoponic and phosphoneus nutrition of plants in Iranian Apetites. Final Research Project (N0. 84179). Iran National Science Foundation (INSF). - Baher, Z., Mirza, M., Ghorbanli, M. and Rezaii, M., 2002. The influence of water stress on plant height, herbal and essential oil yield and composition in Satureja hortensis L. Flavour and Fragrance Journal, 17: 275-277. - Bates, L.S., Waldern, R.P. and Tear, I.D., 1973. Rapid determination of free proline for water stress studies. Plant and Soil, 39: 205-207. - Bernath, J., 2002. Strategies and recent achievements in selection of medicinal and aromatic plants. Acta Horticulture, 576: 115-128. - Bernstein, N., Kravchik, M. and Dudai, N., 2010. Salinity‐induced changes in essential oil, pigments and salts accumulation in sweet basil (Ocimum basilicum) in relation to alterations of morphological development. Annals of Applied Biology, 156: 167-177. - Charles, D., Joly, R. and Simon, J., 1990. Effect of osmotic stress on the essential oil content and composition of peppermint. Phytochemical Analysis, 29: 2837-2840. - Conner, J. and Hartl, D., 2004. A primer of ecological genetics. Sinauer Associates, Sunderland, 304p. - Cristie, B.R. and hattuk, V., 1992. The diallel cross: design, analysis, and use for plant breeders: 9-36. In: Janick, J., (Ed.). Plant Breeding Review. John Wiley & Sons, 402p. - Emami Bistgani, Z., Hashemi, M., DaCostab, M., Crakerb, L., Maggic, F. and Morshedloo, M., 2019. Effect of salinity stress on the physiological characteristics, phenolic compounds and antioxidant activity of Thymus vulgaris L. and Thymus daenensis Celak. Industrial Crops and Products, 135: 311-320. - Ezz El-Din, A., Aziz, E., Hendawy, S. and Omer, E., 2009. Response of Thymus vulgaris L. to salt stress and alar (B9) in newly reclaimed soil. Journal Applied Science Research, 5(12): 2165-2170. - Falconer, D.S. and Mackay, T., 1996. Introduction to Quantitative Genetics. Longman, London, 464p. - FAO., 2021. Global Soil Partnership. Soil Salinity. Available at http://www.fao.org/global-soil-partnership/areas-of-work/soil-salinity/en/ - Ghasemi Pirbalouti, A., Hashemi, M. and Taherian Ghahfarokhi, F., 2013. Essential oil and chemical compositions of wild and cultivated Thymus daenensis Celak and Thymus vulgaris L. Industrial crops and products, 48: 43-48. - Hassiotisa, C.N., Tarantilis, P.A., Daferera, D. and Polissiou, M.G., 2010. Etherio, a new variety of Lavandula angustifolia with improved essential oil production and composition from natural selected genotypes. Industrial Crops and Products, 32: 77-82. - Hendawy, S. and Khalid, Kh., 2005. Response of sage (Salvia officinalis L.) plants to zinc application under different salinity levels. Journal of Applied Sciences Research, 1: 147-155. - Heuberger, H., 2010. Cultivation and breeding of Chinese medicinal plant in Germany. Planta Medica. 76(17): 1956-1962. - Hornok, L., 1996. Essential oil of Matricaria chamomilla affected by irrigation regime and nitrogen in in two cultivars. Journal of American Society of Horticultural Science, 13: 169-175. - Hosseini, H., Mousavi-Fard, S., Fatehi, F. and Qaderi, A., 2015. Changes in phytochemical and morpho-physilogical traits of thyme (Thymus vulgaris CV Varico 3) under different salinity levels. Iranian Journal of Medicinal Plants, 16(61): 22-33. - Iapichino, G., Arnone, C., Bertolini, M. and Roxas, U., 2006. Propagation of three Thymus species by stem cuttings. Acta Horticalture, 723: 411-414. - Immanuel, S.C., Pothiraj, N., Thiyagarajan, K., Bharathi, M. and Rabindran, R., 2011. Genetic parameters of variability, correlation and path coefficient studies for grain yield and other yield attributes among rice blast disease resistant genotypes of rice (Oryza sativa L.). African Journal of Biotechnology, 10(17): 3322 3334. - Jaleel, C., Gopi, R., Sankar, B., Manivannan, P., Kishorekumar, A. and Sridharan, R., 2007. Studies on germination, seedling vigour, lipid peroxidation and proline metabolism in Catharanthus roseus seedlings under salt stress. South African Journal Botany, 73: 190-195. - Jaimand, K. and Rezaee, B., 2004. Investigation on chemical constituents of essential oils from Achillea eriophora DC by Distillation methods. Iranian Journal of Medicinal and Aromatic Plants Research, 20(1): 89-98. - Jamzad, Z., 2010. Thymus and Satureja species of Iran. Institute Research Forests and Rangeland, (In Persian), 176p. - Khoshsokhan, F., Babalar, M., Fatahi, M. and Poormeidani, A., 2014. Assessment of genetic diversity of some wild populations of Thymus Kotschyanus using RAPD molecular markers. Cercetari Agronomice in Moldova., 3: 71-81. - Lothrop, J.E., Atkins, R.E. and Smith, O.S, 1985. Variability for yield and yield components in IAPIR grain sorghum random mating population II. Correlations, estimated gain from selection, and correlated responses to selection. Crop Science, 25: 240-244. - Mohammadi, M., Tabrizi, L., Shokrpour, M., Hadian, J. and Schulz, H., 2019. Study on general combining ability of morphological and phytochemical traits in different populations of Thymus daenensis in polycross test. Iranian Journal of Horticultural Science, 51(2): 295-305. - Mohammadi, M., Tabrizi, L., Shokrpour, M., Hadian, J. and Schulz, H., 2020. Morphological and phytochemical screening of some Thymus ecotypes (Thymus spp.) native to Iran in order to select elite genotypes. Journal of Applied Botany and Food Quality, 93: 186-196. - Monirifar, H., 2010. Half-sib progeny test for selection of best parents for development of a synthetic variety of alfalfa. Iranian Journal of Crop Sciences, 12: 66-75. - Morales, R., 2002. The History, Botany and Taxonomy of the Genus Thymus. CRC Press, 43p. - Mousa, N.A., Siaguru, P., Wiryowidagdo, S. and Wagih, M., 2007. Evaluation and selection of elite clonal genotypes of the sweet crop licorice (Glycyrrhiza glabra) in a new environment. Sugar Tech, 9: 83-94. - Munns, R. and Gilliham, M., 2015. Salinity tolerance of crops-what is the cost? New Phytologist, 208(3): 668-673. - Nguyen, H.T. and Sleeper, A., 1983. Theory and application of half-sib matings in forage breeding. Theoretical and Applied Genetics, 64: 187-196. - Ozturk, A., Unlukara, A., Ipek, A. and Gurbuz, B., 2004. Effects of salt stress and water deficit on plant growth and essential oil content of lemon balm (Melissa officinalis L.). Pakistan Journal Botany, 36: 787-792. - Pharmacopoeia, B., 1988. Bernan Press. London: HMSO, 2: 137-138. - Rahdari, P., Tavakoli, S. and Hosseini, S., 2012. Studying of salinity stress effect on germination, proline, sugar, protein, lipid and chlorophyll content in purslane (Portulaca oleracea L.) leaves. Journal of Stress Physiology & Biochemistry, 8(1): 182-193. - Rahimi, M., Aharizad, S. and Mohebalipour, N., 2017. Evaluation of genetic diversity in populations of lemon balm (Melissa officinalis L.) in terms of agronomic traits, essential oil and citral concentration. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 25(2): 271-288. - Rahimmalek, M., Bahreininejad, B., Khorrami, M. and Tabatabaei, S., 2009. Genetic variability and geographic differentiation in Thymus daenensis subsp. daenensis, an endangered medicinal plant, as revealed by inter-simple sequence repeat (ISSR) markers. Biochemistry Genetic, 47: 831-842. - Riaz, P., Surendra, S. and Harting, H., 2007. Effect of irrigation intervals and splitted nitrogen on carvone content of Anethum graveolense L. grown in semi-arid region. Journal of Horticulture Environment Biotechnology, 41(4): 25-30. - Rosielle, A.A. and Hamblin, J., 1981. Theoretical aspects of selection for yield in stress and non-stress environment. Crop science, 21(6): 943-946. - Rustaiee, A., Yavari, A., Nazeri, V., Shokrpour, M., Sefidkon, F. and Rasouli, M., 2013. Genetic diversity and chemical polymorphism of some Thymus species. Chemistry and Biodiversity, 10(6): 1088-1098. - Salamati, M. and Yousefi, M., 2014. Evaluation of variation for yield and morphological traits in Dracocephalum moldavica L. genotypes. Journal of Plant Research, 27(1): 91-99. - Santos, C., 2004. Regulation of chlorophyll biosynthesis and degradation by salt stress in sunflower leaves. Scienctica Horticulture, 103: 93-99. - Siringam, K., Juntawong, N., Cha-um, S. and Kirdmanee, C., 2011. Salt stress induced ion accumulation, ion homeostasis, membrane injury and sugar contents in salt-sensitive rice (Oryza sativa L. spp. indica) roots under isoosmotic conditions. African Journal of Biotechnology, 10(8): 1340-1346. - Stansfield, W.D., 1991. Theory and Problems in Genetics. McGraw-Hill, New York, USA, 392p. - Sultana, N., Ikeda, T. and Itoh, R., 1999. Effect of NaCl salinity on photosynthesis and dry matter accumulation in developing rice grains. Environmental and Experimental Botany, 42: 211-220. - Talle, B., Darvish, F., Mohammadi, A., Abbaszadeh, B. amd Rohami, M., 2012. Assessment of relationship between effective traits on yield and compounds of essential oil and morphological traits of lemon balm (Melissa officinalis L.) accessions using path analysis and canonical correlation. Journal of Basic and Applied Scientific Research, 2(4): 3719-3723. - Thompson, J., 2002. Population structure and the spatial dynamics of genetic polymorphism in thyme: 45-74. In: Stahl-Biskup, E. and Saez, F., (Eds.). Thyme: The Genus Thymus. CRC Press, 352p. - Tohidi, B., Rahimmalek, M. and Arzani, A., 2018. Variations in chemical composition and bioactive compounds of Thymus kotschyanus Boiss. & Hohen populations originated from different collection sites. Journal Essential Oil Bearing Plants, 21(5): - Udagawa, Y., Ito, T., Tognoni, F., Nukaya, A. and Maruo, T., 1995. Some responses of dill (Anethum graveolens) and thyme (Thymus vulgaris), grown in hydroponics to the concentration of nutrient solution. Acta Horticulture, 396: 203-210. - Wricke, G. and Weber, W.E., 1986. Quantitative Genetic and Selection in Plant Breeding. Walter De Gruyter. New York, 421p. - Yavari, A., Shokrpour, M., Tabrizi L. and Hadian, J., 2015. Analysis of morphological variation and general combining ability in half sib families of Echinacea purpurea L. Iranian Journal of Horticultural Science, 47(4): 617-630. (in Farsi) | ||
|
آمار تعداد مشاهده مقاله: 1,708 تعداد دریافت فایل اصل مقاله: 582 |
||