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Abbas Marhoon, I, Majid Al-Musawi, M. (1402). Evaluation of the Efficacy of Sodium Chloride Nanoparticles on the Vitality of Leishmania Major (in vitro). سامانه مدیریت نشریات علمی, 78(2), 627-632. doi: 10.22092/ari.2022.359469.2425
I Abbas Marhoon; M Majid Al-Musawi. "Evaluation of the Efficacy of Sodium Chloride Nanoparticles on the Vitality of Leishmania Major (in vitro)". سامانه مدیریت نشریات علمی, 78, 2, 1402, 627-632. doi: 10.22092/ari.2022.359469.2425
Abbas Marhoon, I, Majid Al-Musawi, M. (1402). 'Evaluation of the Efficacy of Sodium Chloride Nanoparticles on the Vitality of Leishmania Major (in vitro)', سامانه مدیریت نشریات علمی, 78(2), pp. 627-632. doi: 10.22092/ari.2022.359469.2425
Abbas Marhoon, I, Majid Al-Musawi, M. Evaluation of the Efficacy of Sodium Chloride Nanoparticles on the Vitality of Leishmania Major (in vitro). سامانه مدیریت نشریات علمی, 1402; 78(2): 627-632. doi: 10.22092/ari.2022.359469.2425

Evaluation of the Efficacy of Sodium Chloride Nanoparticles on the Vitality of Leishmania Major (in vitro)

Archives of Razi Institute
مقاله 16، دوره 78، شماره 2، خرداد و تیر 2023، صفحه 627-632    اصل مقاله (446.07 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359469.2425
نویسندگان
I Abbas Marhoon* 1؛ M Majid Al-Musawi2
1Department of Biology, College of Education, University of Al-Qadisiyah, Al-Qadisiyah, Iraq
2Department of Biology, Faculty of Education for Girls, University of Kufa, Kufa, Iraq
چکیده
Leishmaniasis is one of the most important zoonotic diseases transmitted to humans by sand flies (Phlebotomus spp). Leishmania major promastigote causes Cutaneous Leishmaniasis in humans. The study aimed to investigate the effectiveness of Sodium Chloride nanoparticles (NaCl NPs) on the vitality of Leishmania major promastigote compared with the standard dose of Pentostam under laboratory conditions. Various concentrations of 2, 4, 6, and 8 μg/ml of the NaCl NPs were prepared. These concentrations were tested in vitro on L. major growth by the culture of the parasite in the cell culture microplate. After the fourth day, a different concentration of NaCl NPs was added with three replicates for each concentration. Later, the numbers of promastigotes were counted daily using a Haemocytometer stained by Trypan blue solution stain duration of the study, which continued for four days. The results showed that the Growth Index (GI) rate of L. major promastigote was decreased with increasing NaCl NPs concentration. The Growth Index rates were 1.32×106, 1.31×106, 0.95×106, and 0.78×106for the mentioned concentrations. These values were compared with the rate of the Pentostam group and control group, which were 1.09×106 and 3.43×106, respectively. The results revealed that the highest inhibition percentage was 92% for 8 μg/ml NaCl NPs after 96 hours, Pentostam group and control group, which were %86 and %0.00 for inhibition promastigote, respectively in the same period. The statistical analysis revealed a significant difference among concentrations at P≤0.05 compared with the Pentostam and control groups. The current study concluded that the NaCl NPs have an excellent biological effect in inhibiting L. major promastigote growth in vitro. These promising results paved the way for employing NaCl NPs to treat human cutaneous leishmaniasis.
کلیدواژه‌ها
Cutaneous leishmaniasis؛ Leishmania major؛ NaCl Nanoparticles؛ Pentostam
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مراجع
  1. Postigo JA. Leishmaniasis in the World Health Organization Eastern Mediterranean Region. Int J Antimicrob Agents. 2010;36(1):62-5.
  2. WHO. The World health report: 2004: changing history: World Health Organization; 2004 .
  3. WHO. Control of the leishmaniases: report of a meeting of the WHO Expert Committee on the Control of Leishmaniases Geneva: World Health Organization; 2010.
  4. Yanik M, Gurel MS, Simsek Z, Kati M. The psychological impact of cutaneous leishmaniasis. Clin Exp Dermatol. 2004;29(5):464-7.
  5. AlSamarai AM, AlObaidi HS. Cutaneous leishmaniasis in Iraq. J Infect Dev Ctries. 2009;3(02):123-9.
  6. Berman JD. Human leishmaniasis: clinical, diagnostic, and chemotherapeutic developments in the last 10 years. Clin Infect Dis. 1997;24(4):684-703.
  7. Chang K-P, Fong D, Bray R. Biology of Leishmania and leishmaniasis. Leishmaniasis. 1985:1-30.
  8. Killick-Kendrick R. The life-cycle of Leishmania in the sandfly with special reference to the form infective to the vertebrate host. Ann Parasitol Hum Comp. 1990;65(1):37-42.
  9. Croft SL. Monitoring drug resistance in leishmaniasis. Trop Med Int Health. 2001;6(11):899-905.
  10. AL-Khan H. Effect of aqueous extracts of Capparis spinosa and Citrullus colocynthis plants on growth and metabolism of Leishmania major promastigotes in vitro. Iraq: College of Science, Mosul University. 2001.
  11. Saporito L, Giammanco GM, De Grazia S, Colomba C. Visceral leishmaniasis: host-parasite interactions and clinical presentation in the immunocompetent and in the immunocompromised host. Int J Infect Dis. 2013;17(8):572-6.
  12. Alamri ZA. Experimental Study on the Efficacy of Syzygium Aromaticum Against Cutaneous Leishmaniasis in Comparison with Pentostam. J med Pharm Sci. 2020;4(2):84-58.
  13. Desjeux P, Ghosh RS, Dhalaria P, Strub-Wourgaft N, Zijlstra EE. Report of the post kala-azar dermal leishmaniasis (PKDL) consortium meeting, New Delhi, India, 27–29 June 2012. Parasit Vectors. 2013;6(1):196.
  14. Igbineweka O, Aghedo F, Idusuyi O, Hussain N. Evaluating the efficacy of topical silver nitrate and intramuscular antimonial drugs in the treatment of cutaneous leishmaniasis in sokoto, Nigeria. African J Clin Exp Microbiol. 2012;13(2):90-7.
  15. al-Bashir NT, Rassam MB, al-Rawi BM. Axenic cultivation of amastigotes of Leishmania donovani and Leishmania major and their infectivity. Ann Trop Med Parasitol. 1992;86(5):487-502.
  16. Jebali A, Kazemi B. Nano-based antileishmanial agents: a toxicological study on nanoparticles for future treatment of cutaneous leishmaniasis. Toxicol In Vitro. 2013;27(6):1896-904.
  17. El-On J, Messer G. Leishmania major: antileishmanial activity of methylbenzethonium chloride. Am J Trop Med Hyg. 1986;35(6):11.
  18. Croft SL, Coombs GH. Leishmaniasis--current chemotherapy and recent advances in the search for novel drugs. Trends Parasitol. 2003;19(11):502-8.
  19. Frezard F, Demicheli C, Ribeiro RR. Pentavalent antimonials: new perspectives for old drugs. Molecules. 2009;14(7):2317-36.
  20. Ansari MJ, Jasim SA, Taban TZ, Bokov DO, Shalaby MN, Al-Gazally ME, et al. Anticancer drug-loading capacity of green synthesized porous magnetic iron nanocarrier and cytotoxic effects against human cancer cell line. J Clust Sci. 2023;34(1):467-77.
  21. Catala A. Lipid peroxidation of membrane phospholipids generates hydroxy-alkenals and oxidized phospholipids active in physiological and/or pathological conditions. Chem Phys Lipids. 2009;157(1):1-11.
  22. Delavari M, Dalimi A, Ghaffarifar F, Sadraei J. In vitro study on cytotoxic effects of ZnO nanoparticles on promastigote and amastigote forms of Leishmania major (MRHO/IR/75/ER). Iran J Parasitol. 2014;9(1):6.
  23. Hafsan H, Bokov D, Abdelbasset WK, Kadhim MM, Suksatan W, Majdi HS, et al. Dietary Dracocephalum kotschyi essential oil improved growth, haematology, immunity and resistance to Aeromonas hydrophila in rainbow trout (Oncorhynchus mykiss). Aquac Res. 2022;53(8):3164-75.
  24. Huldani H, Abdalkareem Jasim S, Olegovich Bokov D, Abdelbasset WK, Nader Shalaby M, Thangavelu L, et al. Application of extracellular vesicles derived from mesenchymal stem cells as potential therapeutic tools in autoimmune and rheumatic diseases. Int Immunopharmacol. 2022;106:108634.
  1. Najim RA, Sharquie KE, Farjou IB. Zinc sulphate in the treatment of cutaneous leishmaniasis: an in vitro and animal study. Mem Inst Oswaldo Cruz. 1998;93(6):831-7.
  2. Palmieri B, Sblendorio V. Oxidative stress tests: overview on reliability and use. Eur Rev Med Pharmacol Sci. 2007;11(6):383-99.
  3. Torabi N, Mohebali M, Shahverdi AR, Rezayat SM, Edrissian GH, Esmaeili J, et al. Nanogold for the treatment of zoonotic cutaneous leishmaniasis caused by Leishmania major (MRHO/IR/75/ER): an animal trial with methanol extract of Eucalyptus camaldulensis. JPHS. 2011.
  4. Zadeh FA, Bokov DO, Salahdin OD, Abdelbasset WK, Jawad MA, Kadhim MM, et al. Cytotoxicity evaluation of environmentally friendly synthesis Copper/Zinc bimetallic nanoparticles on MCF-7 cancer cells. Rend Lincei Sci Fis Nat. 2022;33(2):441-7.
  5. Zainab I, Mohammed M, Qasim T. Hormonal profile of men during infertility. Biochem Cell Arch. 2021;21(1):2895-8.
  6. Zhang L, Gu FX, Chan JM, Wang AZ, Langer RS, Farokhzad OC. Nanoparticles in medicine: therapeutic applications and developments. Clin Pharmacol Ther. 2008;83(5):761-9.
 

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