اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,073
تعداد مقالات34,300
تعداد مشاهده مقاله47,097,441
تعداد دریافت فایل اصل مقاله78,005,558
Saleh Ahmed, S, Ghanim Abdulwahhab, I, Salman Alagely, H. (1401). Genetic Detection of Genes Encodes Some Enzymes in Entamoebahistolytica in Diarrhea Children in Iraq. سامانه مدیریت نشریات علمی, 77(6), 2201-2206. doi: 10.22092/ari.2022.359133.2375
S Saleh Ahmed; I Ghanim Abdulwahhab; H Salman Alagely. "Genetic Detection of Genes Encodes Some Enzymes in Entamoebahistolytica in Diarrhea Children in Iraq". سامانه مدیریت نشریات علمی, 77, 6, 1401, 2201-2206. doi: 10.22092/ari.2022.359133.2375
Saleh Ahmed, S, Ghanim Abdulwahhab, I, Salman Alagely, H. (1401). 'Genetic Detection of Genes Encodes Some Enzymes in Entamoebahistolytica in Diarrhea Children in Iraq', سامانه مدیریت نشریات علمی, 77(6), pp. 2201-2206. doi: 10.22092/ari.2022.359133.2375
Saleh Ahmed, S, Ghanim Abdulwahhab, I, Salman Alagely, H. Genetic Detection of Genes Encodes Some Enzymes in Entamoebahistolytica in Diarrhea Children in Iraq. سامانه مدیریت نشریات علمی, 1401; 77(6): 2201-2206. doi: 10.22092/ari.2022.359133.2375

Genetic Detection of Genes Encodes Some Enzymes in Entamoebahistolytica in Diarrhea Children in Iraq

Archives of Razi Institute
مقاله 23، دوره 77، شماره 6، بهمن و اسفند 2022، صفحه 2201-2206    اصل مقاله (484.94 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359133.2375
نویسندگان
S Saleh Ahmed1؛ I Ghanim Abdulwahhab* 1؛ H Salman Alagely2
1College of Education, University of Tikrit, Tikrit, Iraq
2Biotechnology Research Center, Al Nahrain University, Baghdad, Iraq
چکیده
Entamoebahistolytica is a protozoan, an anaerobic intestinal parasite that causes about 50 million infections and a mortality rate of more than 100,000 worldwide. For diagnosis, two hundred samples of children with diarrhea signs were evaluated using staining and polymerase chain reaction techniques. The current study recorded 11 positive cases of E. histolytica, which were diagnosed by polymerase chain reaction (PCR) out of a total of 51 positive cases diagnosed microscopically for pediatric children arriving at Tikrit General Hospital in Tikrit city and the nearby areas. The percentage of positive cases reached 21.57% for the PCR assay, as significant differences appeared compared to the microscopic examination. The results showed that the parasite infection rates differed between males (54.9%) and females (45.1%). The percentage of infected numbers in the age group less than one year was about 43.1%, while the percentage of disease control and prevention programs f infected people in the age group 1-2 years was (31.4%). The results showed that the percentage of infected age group between (2-3) years was 15.7%. The recorded data showed that 5.9% and 3.9% of the participants were infected in the age group of3-4 and over four years old, respectively. The genes encoded in Cysteine proteinase five and Phospholipase were diagnosed using the PCR technique. The concordance with the current study isolate and 90% match globally. In conclusion, the methods of detection of E. histolytica appeared differences in positive results for this parasite.
کلیدواژه‌ها
Entamoebahistolytica؛ Cysteine proteinase 5؛ Phospholipase
سایر فایل های مرتبط با مقاله
مراجع
  1. Kadir MA, Daoud IS, Al-Bayati Z. Differentiation between Entamoeba histolytica and E. dispar using enzyme-linke immunosorbent assay and wet mount method. Tirkit J Pharm Sci. 2013;9:122-9.
  2. Rostamighalehjaghi S, Jamali R, Rezaie S, Babaei Z, Hooshyar H, Rezaeian M. Evaluation of a Single PCR Assays on Cp5 Gene for Differentiation of Entamoeba histolytica and E. dispar. Iran J Public Health. 2010;39(4):64.
  3. Kim H-J, Park S-H, Lee T-H, Nahm B-H, Kim Y-R, Kim H-Y. Microarray detection of food-borne pathogens using specific probes prepared by comparative genomics. Biosens Bioelectron. 2008;24(2):238-46.
  4. Bensoussan E, Nasereddin A, Jonas F, Schnur LF, Jaffe CL. Comparison of PCR assays for diagnosis of cutaneous leishmaniasis. J Clin Microbiol. 2006;44(4):1435-9.
  1. Al-Difaie RSS, Al-Kaeebi SRA. PCR conventional for detecting AP and PLA virulence Use factors of Entamoeba histolytica in patients stool samples in Al-Qadisiyah Province. Wasit J Sci Med. 2016: 8(4): 102- 110.
  2. Brown TA. Mutation, repair and recombination. Genomes 2nd edition: Wiley-Liss; 2002.
  3. Shakir OM, Abdulwahhab IG. Study of the Effect of Klebsiella pneumoniae Antigens on the Histological Structure of the Kidney in Laboratory Rabbits Infected with Entamoeba histolytica. Tikrit J Agric Sci.2019;18(4):139-45.
  4. Mozer S, Abdulwahhab IG, AL-Azaawie AF. Diagnosis Of Contamination Of Vegetables And Fruits With Cryptosporidium Spp Using Nested-PCR Technique. Nat Volatiles Essent Oils. 2021:223-32.
  5. Pham Duc P, Nguyen-Viet H, Hattendorf J, Zinsstag J, Dac Cam P, Odermatt P. Risk factors for Entamoeba histolytica infection in an agricultural community in Hanam province, Vietnam. Parasit Vector. 2011;4(1):1-9.
  6. Green MR, Sambrook J. Nested polymerase chain reaction (PCR). Cold Spring Harbor Protocols. 2019;2019(2):095182.
  7. Nari G, JL CV, JL BR. Amebic liver abscess. Three years experience. Rev Esp Enferm Dig. 2008;100(5):268-72.
  8. Mozer S, Abdulwahhab IG, AL-Azaawie AF. Extraction of the DNA of Giardia lamblia isolated from vegetables and fruits in a simplified way and its diagnosis using Nested-PCR. J Parasit Dis. 2022:1-5.
  9. Al-Damerchi ATN, Al-Ebrahimi HN. Detection of major virulence factor of Entamoeba histolytica by using PCR technique. Al-Qadisiyah Med J. 2016;12(21):36-45.
  10. Tanyuksel M, Petri Jr WA. Laboratory diagnosis of amebiasis. Clin Microbiol Rev. 2003;16(4):713-29.
آمار
تعداد مشاهده مقاله: 14,381
تعداد دریافت فایل اصل مقاله: 56,969


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب