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Alwaily, E. R, Abood, M. S, Al Uobody, R. M. (1401). Diagnosis of Oral candidiasis in Patients under 12 Years: 18S rRNA as a Marker of Molecular Characterization of Candida tropicalis. سامانه مدیریت نشریات علمی, 78(1), 475-483. doi: 10.22092/ari.2022.359466.2422
E. R Alwaily; M. S Abood; R. M Al Uobody. "Diagnosis of Oral candidiasis in Patients under 12 Years: 18S rRNA as a Marker of Molecular Characterization of Candida tropicalis". سامانه مدیریت نشریات علمی, 78, 1, 1401, 475-483. doi: 10.22092/ari.2022.359466.2422
Alwaily, E. R, Abood, M. S, Al Uobody, R. M. (1401). 'Diagnosis of Oral candidiasis in Patients under 12 Years: 18S rRNA as a Marker of Molecular Characterization of Candida tropicalis', سامانه مدیریت نشریات علمی, 78(1), pp. 475-483. doi: 10.22092/ari.2022.359466.2422
Alwaily, E. R, Abood, M. S, Al Uobody, R. M. Diagnosis of Oral candidiasis in Patients under 12 Years: 18S rRNA as a Marker of Molecular Characterization of Candida tropicalis. سامانه مدیریت نشریات علمی, 1401; 78(1): 475-483. doi: 10.22092/ari.2022.359466.2422

Diagnosis of Oral candidiasis in Patients under 12 Years: 18S rRNA as a Marker of Molecular Characterization of Candida tropicalis

Archives of Razi Institute
مقاله 59، دوره 78، شماره 1، فروردین و اردیبهشت 2023، صفحه 475-483    اصل مقاله (586.49 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359466.2422
نویسندگان
E. R Alwaily* 1؛ M. S Abood2؛ R. M Al Uobody3
1Microbiology Research Group , College of Pharmacy, Al-Ayen University, Thi-Qar, Iraq
2Department of Biology, College of Education for Pure Science, Thi-Qar University, Thi-Qar 64001, Iraq
3College of Pharmacy, Al-Ayen University, Thi-Qar, 64001, Iraq
چکیده
Candida tropical has been found as the most abundant pathogenic yeast species under the group Candida-non-albicans. Despite this, it is taxonomically related to C. albicans and has many of its pathogenic characteristics. Infection with Candida tropicalis is closely associated with many virulence factors encoded by multiple virulence genes. This study aims to diagnose C. tropicalis based on the presence of 18SrRNA and to detect many virulence genes. C. tropicalis isolates were collected from oral candidiasis patients. Children infected with oral thrush ranging in age from infants to 12 years old provided 150 samples. C. albicans (66.68 %), C. tropicalis (13.21 %), C. krusie (9.43 %), C. parapsilosis (7.55 %), and C. glarata were isolated as C. tropicalis types, according to the findings of the present study (2.83%). The presence of the 18SrRNA gene was confirmed in the isolates. All isolates were positive for cph1 and hwp1, while some were positive for sap1 (78.5%) and plb1 genes (71.4%). Using sequences and phylogenetic trees, it was determined that there was negligible genetic variation between local isolates and global strains. These virulence factor genes play a crucial role in developing infections.
کلیدواژه‌ها
Oral candidiasis؛ sap1؛ hwp1؛ cph1؛ plb1
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مراجع
  1. Hu L, He C, Zhao C, Chen X, Hua H, Yan Z. Characterization of oral candidiasis and the Candida species profile in patients with oral mucosal diseases. Microb Pathog. 2019;134:103575.
  2. Chamorro-Petronacci C, Garcia-Garcia A, Lorenzo-Pouso AI, Gomez-Garcia FJ, Padin-Iruegas ME, Gandara-Vila P, et al. Management options for low-dose methotrexate-induced oral ulcers: A systematic review. Med Oral Patol Oral Cir Bucal. 2019;24(2):e181-e9.
  3. Bensadoun RJ, Patton LL, Lalla RV, Epstein JB. Oropharyngeal candidiasis in head and neck cancer patients treated with radiation: update 2011. Support Care Cancer. 2011;19(6):737-44.
  4. Sardi JCO, Scorzoni L, Bernardi T, Fusco-Almeida AM, Mendes Giannini MJS. Candida species: current epidemiology, pathogenicity, biofilm formation, natural antifungal products and new therapeutic options. J Med Microbiol. 2013;62(Pt 1):10-24.
  5. Zuza-Alves DL, Silva-Rocha WP, Chaves GM. An Update on Candida tropicalis Based on Basic and Clinical Approaches. Front Microbiol. 2017;8:1927.
  6. Chai LY, Denning DW, Warn P. Candida tropicalis in human disease. Crit Rev Microbiol. 2010;36(4):282-98.
  7. Kothavade RJ, Kura MM, Valand AG, Panthaki MH. Candida tropicalis: its prevalence, pathogenicity and increasing resistance to fluconazole. J Med Microbiol. 2010;59(8):873-80.
  8. Gupta V, Abhisheik K, Balasundari S, Devendra NK, Shadab K, Anupama M. Identification of Candida albicans using different culture media and its association in leukoplakia and oral squamous cell carcinoma. J Oral Maxillofac Pathol. 2019;23(1):28.
  9. Fatima A, Bashir G, Wani T, Jan A, Kohli A, Khan MS. Molecular identification of Candida species isolated from cases of neonatal candidemia using polymerase chain reaction-restriction fragment length polymorphism in a tertiary care hospital. Indian J Pathol Microbiol. 2017;60(1):61.
  10. Bryman A, Cramer D. Quantitative data analysis with IBM SPSS (21): A guide for social scientists Rutledge. 2012.
  11. Ambe NF, Longdoh NA, Tebid P, Bobga TP, Nkfusai CN, Ngwa SB, et al. The prevalence, risk factors and antifungal sensitivity pattern of oral candidiasis in HIV/AIDS patients in Kumba District Hospital, South West Region, Cameroon. Pan Afr Med J. 2020;36:23.
  12. Muadcheingka T, Tantivitayakul P. Distribution of Candida albicans and non-albicans Candida species in oral candidiasis patients: Correlation between cell surface hydrophobicity and biofilm forming activities. Arch Oral Biol. 2015;60(6):894-901.
  13. Costa CR, Passos XS, e Souza LK, Lucena Pde A, Fernandes Ode F, Silva Mdo R. Differences in exoenzyme production and adherence ability of Candida spp. isolates from catheter, blood and oral cavity. Rev Inst Med Trop Sao Paulo. 2010;52(3):139-43.
  14. Fricke S, Fricke C, Schimmelpfennig C, Oelkrug C, Schonfelder U, Blatz R, et al. A real-time PCR assay for the differentiation of Candida species. J Appl Microbiol. 2010;109(4):1150-8.
  15. Schabereiter-Gurtner C, Selitsch B, Rotter ML, Hirschl AM, Willinger B. Development of novel real-time PCR assays for detection and differentiation of eleven medically important Aspergillus and Candida species in clinical specimens. J Clin Microbiol. 2007;45(3):906-14.
  16. Zhang J, Hung GC, Nagamine K, Li B, Tsai S, Lo SC. Development of Candida-Specific Real-Time PCR Assays for the Detection and Identification of Eight Medically Important Candida Species. Microbiol Insights. 2016;9:21-8.
  17. Sanita PV, Zago CE, Mima EG, Pavarina AC, Jorge JH, Machado AL, et al. In vitro evaluation of the enzymatic activity profile of non-albicans Candida species isolated from patients with oral candidiasis with or without diabetes. Oral Surg Oral Med Oral Pathol Oral Radiol. 2014;118(1):84-91.
  18. Parra-Ortega B, Cruz-Torres H, Villa-Tanaca L, Hernandez-Rodriguez C. Phylogeny and evolution of the aspartyl protease family from clinically relevant Candida species. Mem Inst Oswaldo Cruz. 2009;104(3):505-12.
  19. Dabiri S, Shams-Ghahfarokhi M, Razzaghi-Abyaneh M. SAP (1-3) gene expression in high proteinase producer Candida species strains isolated from Iranian patients with different candidosis. J Pure Appl Microbiol. 2016;10(3):1891-6.
  20. Zaugg C, Borg-Von Zepelin M, Reichard U, Sanglard D, Monod M. Secreted aspartic proteinase family of Candida tropicalis. Infect Immun. 2001;69(1):405-12.
  21. Das KH, Mangayarkarasi V, Sen M. Genotypic Patterns of Secreted Aspartyl Proteinase Gene in Various Candida Species Isolated from Antenatal Women with Vulvovaginal Candidiasis. J Pure Appl Microbiol. 2019.
  22. Bianchi CM, Bianchi HA, Tadano T, Paula CR, Hoffmann-Santos HD, Leite DP, Jr., et al. Factors Related to Oral Candidiasis in Elderly Users and Non-Users of Removable Dental Prostheses. Rev Inst Med Trop Sao Paulo. 2016;58:17.
  23. Schaller M, Borelli C, Korting HC, Hube B. Hydrolytic enzymes as virulence factors of Candida albicans. Mycoses. 2005;48(6):365-77.
  24. Staniszewska M, Bondaryk M, Piłat J, Siennicka K, Magda U, Kurzątkowski W. Czynniki zjadliwości Candida albicans. Przegl Epidemiol. 2012;66:629-33.
  25. Bassyouni RH, Wegdan AA, Abdelmoneim A, Said W, AboElnaga F. Phospholipase and Aspartyl Proteinase Activities of Candida Species Causing Vulvovaginal Candidiasis in Patients with Type 2 Diabetes Mellitus. J Microbiol Biotechnol. 2015;25(10):1734-41.
  26. Galan-Ladero MA, Blanco MT, Sacristan B, Fernandez-Calderon MC, Perez-Giraldo C, Gomez-Garcia AC. Enzymatic activities of Candida tropicalis isolated from hospitalized patients. Med Mycol. 2010;48(1):207-10.
  27. Jiang C, Li Z, Zhang L, Tian Y, Dong D, Peng Y. Significance of hyphae formation in virulence of Candida tropicalis and transcriptomic analysis of hyphal cells. Microbiol Res. 2016;192:65-72.
  1. Biasoli MS, Tosello ME, Luque AG, Magaro HM. Adherence, colonization and dissemination of Candida dubliniensis and other Candida species. Med Mycol. 2010;48(2):291-7.
  2. Lyon JP, de Resende MA. Correlation between adhesion, enzyme production, and susceptibility to fluconazole in Candida albicans obtained from denture wearers. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2006;102(5):632-8.
  3. Naglik JR, Fostira F, Ruprai J, Staab JF, Challacombe SJ, Sundstrom P. Candida albicans HWP1 gene expression and host antibody responses in colonization and disease. J Med Microbiol. 2006;55(Pt 10):1323-7.
  4. Modrzewska B, Kurnatowski P. Adherence of Candida sp. to host tissues and cells as one of its pathogenicity features. Annals of parasitology. 2015;61(1).
  5. Wan Harun WH, Jamil NA, Jamaludin NH, Nordin MA. Effect of Piper betle and Brucea javanica on the Differential Expression of Hyphal Wall Protein (HWP1) in Non-Candida albicans Candida (NCAC) Species. Evid Based Complement Alternat Med. 2013;2013:397268.
  6. ten Cate JM, Klis FM, Pereira-Cenci T, Crielaard W, de Groot PW. Molecular and cellular mechanisms that lead to Candida biofilm formation. J Dent Res. 2009;88(2):105-15.
  7. de Barros PP, Rossoni RD, Freire F, Ribeiro FC, Lopes L, Junqueira JC, et al. Candida tropicalis affects the virulence profile of Candida albicans: an in vitro and in vivo study. Pathog Dis. 2018;76(2).
  8. Senanayake I, Rathnayaka A, Marasinghe D, Calabon M, Gentekaki E, Lee H, et al. Morphological approaches in studying fungi: Collection, examination, isolation, sporulation and preservation. Mycosphere. 2020;11(1):2678-754
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