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Miri Mizher Al Muhana, B, Ali, S. A, Sabri Jabbar, A. (1401). Isolation of Pseudomonas aeruginosa from Persistent Bacterial Coinfection of a COVID-19 Patients with Molecular Detection of Antibiotics Resistance Genes. سامانه مدیریت نشریات علمی, 78(1), 397-403. doi: 10.22092/ari.2022.359035.2360
B Miri Mizher Al Muhana; S. A Ali; A Sabri Jabbar. "Isolation of Pseudomonas aeruginosa from Persistent Bacterial Coinfection of a COVID-19 Patients with Molecular Detection of Antibiotics Resistance Genes". سامانه مدیریت نشریات علمی, 78, 1, 1401, 397-403. doi: 10.22092/ari.2022.359035.2360
Miri Mizher Al Muhana, B, Ali, S. A, Sabri Jabbar, A. (1401). 'Isolation of Pseudomonas aeruginosa from Persistent Bacterial Coinfection of a COVID-19 Patients with Molecular Detection of Antibiotics Resistance Genes', سامانه مدیریت نشریات علمی, 78(1), pp. 397-403. doi: 10.22092/ari.2022.359035.2360
Miri Mizher Al Muhana, B, Ali, S. A, Sabri Jabbar, A. Isolation of Pseudomonas aeruginosa from Persistent Bacterial Coinfection of a COVID-19 Patients with Molecular Detection of Antibiotics Resistance Genes. سامانه مدیریت نشریات علمی, 1401; 78(1): 397-403. doi: 10.22092/ari.2022.359035.2360

Isolation of Pseudomonas aeruginosa from Persistent Bacterial Coinfection of a COVID-19 Patients with Molecular Detection of Antibiotics Resistance Genes

Archives of Razi Institute
مقاله 49، دوره 78، شماره 1، فروردین و اردیبهشت 2023، صفحه 397-403    اصل مقاله (555.51 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.359035.2360
نویسندگان
B Miri Mizher Al Muhana* 1؛ S. A Ali2؛ A Sabri Jabbar2
1Veterinary Microbiology Department, College of Veterinary Medicine, Al-Qadisiyah University, Al-Qadisiyah, Iraq
2Clinical Laboratory Sciences Department, College of Pharmacology, Al-Qadisiyah University, Al-Qadisiyah, Iraq
چکیده
Pseudomonas aeruginosa (P. aeruginosa) have a considerable risk to public health in the world, due to its high ability to develop resistance to different classes of antibiotics. It has been discovered as a prevalent coinfection pathogen that causes sickness exacerbation in COVID-19 patients. This study aimed to determine the prevalence of P. aeruginosa from COVID-19 patients in Al Diwaniyah province, Iraq and to identify its genetic resistance pattern. 70 clinical samples were obtained from severe cases of patients (RT-PCR positive for SARS-COV-2 on a nasopharyngeal swab) who attended Al Diwaniyah Academic Hospital. 50 P. aeruginosa bacterial isolates were detected via microscopic examination, routine cultured and biochemical testing, then validated by the VITEK-2 compact system. VITEK reported 30 positive results, which later confirmed through molecular detection using 16s RNA specific for detection and a phylogenetic tree.20 isolates had positive PCR findings and 5 isolates submitted to GenBank with accession numbers OL314557.1, OL314556.1, OL314555.1, OL314554.1, OL314553.1.For antibiotic resistance genes, the number of the isolates containing blaOXA-1 and blaCTX-M 18 (90 percent) and 16 (80 percent) respectively. To study its adaptation in a SARS-CoV-2 infected environment, genomic sequencing investigations were undertaken with phenotypic validation. In conclusion, we demonstrate that multidrug resistant P. aeruginosa play an important role in in vivo colonization in COVID-19 patients and could be one of the causes of death of these patients which indicates the great challenge to clinicians in the facing of this serious disease.
کلیدواژه‌ها
COVID-19؛ Pseudomonas aeruginosa؛ bla OXA-1, bla CTX-M؛ antibiotic resistance؛ coinfection
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مراجع
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