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Abid Fazaa ALmiyah, S. (1401). Detection of AcrA and AcrB Efflux Pumps in Multidrug-Resistant Klebsiella pneumonia that Isolated from Wounds Infection Patients in Al-Diwaniyah Province. سامانه مدیریت نشریات علمی, 78(1), 269-276. doi: 10.22092/ari.2022.358956.2342
S Abid Fazaa ALmiyah. "Detection of AcrA and AcrB Efflux Pumps in Multidrug-Resistant Klebsiella pneumonia that Isolated from Wounds Infection Patients in Al-Diwaniyah Province". سامانه مدیریت نشریات علمی, 78, 1, 1401, 269-276. doi: 10.22092/ari.2022.358956.2342
Abid Fazaa ALmiyah, S. (1401). 'Detection of AcrA and AcrB Efflux Pumps in Multidrug-Resistant Klebsiella pneumonia that Isolated from Wounds Infection Patients in Al-Diwaniyah Province', سامانه مدیریت نشریات علمی, 78(1), pp. 269-276. doi: 10.22092/ari.2022.358956.2342
Abid Fazaa ALmiyah, S. Detection of AcrA and AcrB Efflux Pumps in Multidrug-Resistant Klebsiella pneumonia that Isolated from Wounds Infection Patients in Al-Diwaniyah Province. سامانه مدیریت نشریات علمی, 1401; 78(1): 269-276. doi: 10.22092/ari.2022.358956.2342

Detection of AcrA and AcrB Efflux Pumps in Multidrug-Resistant Klebsiella pneumonia that Isolated from Wounds Infection Patients in Al-Diwaniyah Province

Archives of Razi Institute
مقاله 35، دوره 78، شماره 1، فروردین و اردیبهشت 2023، صفحه 269-276    اصل مقاله (507.87 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.358956.2342
نویسنده
S Abid Fazaa ALmiyah*
Biology Department, Collage of Science, AL-Qadisiyah University, Ad Diwaniyah, Iraq
چکیده
Many infections produced by multidrug-resistant (MDR) Klebsiella pneumoniae are the main cause of death and treatment restrictions worldwide. In K. pneumoniae, the efflux pump system is dangerous in drug resistance. Therefore, this study was designed to investigate the involvement of the AcrA and AcrB efflux pumps in antibiotic resistance in Klebsiella pneumoniae isolated from wound patients. During June 2021-February 2022, 87 clinical isolates of Klebsiella pneumonia bacteria were obtained from wound samples patients consulted to the hospitals in AL-Diwaniyah province, Iraq. The disc diffusion method performed an antibiotic susceptibility test after microbiological/biochemical identification. The polymerase chain reaction (PCR) technique was used to examine efflux genes' prevalence (acrA and acrB). The results showed that resistance to Carbenicillin 72 (82.7%), Erythromycin 66 (75.8%), Rifampin 58 (66.6%), Ceftazidime 52 (59.7%), Cefotaxime 44 (50.5%), Novobiocin 38 (43.6%), Tetracycline 32 (36.7%), Ciprofloxacin 22 (25.2%), Gentamicin 16 (18.3%), Nitrofurantoin 6 (10.3%) in Klebsiella pneumoniae isolates. The PCR procedure revealed that the occurrence of the acrA and acrB genes is 55 (100%) and 55 (100%), respectively. The findings of this investigation show that the AcrA and AcrB efflux pumps play a crucial character in antibiotic resistance in multidrug-resistant Klebsiella pneumoniae bacterial isolates. As a result of the unintentional transmission of antimicrobial resistance genes, precise detection of resistance genes using molecular approaches is required to switch the extent of resistant strains.
کلیدواژه‌ها
Efflux pumps؛ AcrA؛ AcrB؛ Multidrug resistance؛ Klebsiella pneumoniae
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مراجع
  1. Ranjbar R, Kelishadrokhi AF, Chehelgerdi M. Molecular characterization, serotypes and phenotypic and genotypic evaluation of antibiotic resistance of the Klebsiella pneumoniae strains isolated from different types of hospital-acquired infections. Infect Drug Resist. 2019;12:603.
  2. Fayez MS, Hakim TA, Agwa MM, Abdelmoteleb M, Aly RG, Montaser NN, et al. Topically applied bacteriophage to control multi-drug resistant klebsiella pneumoniae infected wound in a rat model. Antibiotics. 2021;10(9):1048.
  3. Torabi LR, Naghavi NS, Doudi M, Monajemi R. Efficacious antibacterial potency of novel bacteriophages against ESBL-producing Klebsiella pneumoniae isolated from burn wound infections. Iran J Microbiol. 2021;13(5):678.
  4. Srinivasan VB, Singh BB, Priyadarshi N, Chauhan NK, Rajamohan G. Role of novel multidrug efflux pump involved in drug resistance in Klebsiella pneumoniae. PloS One. 2014;9(5):e96288.
  5. Ferreira RL, da Silva B, Rezende GS, Nakamura-Silva R, Pitondo-Silva A, Campanini EB, et al. High prevalence of multidrug-resistant Klebsiella pneumoniae harboring several virulence and β-lactamase encoding genes in a Brazilian intensive care unit. Front Microbiol. 2019;9:3198.
  6. Kareem SM, Al-Kadmy IM, Kazaal SS, Ali ANM, Aziz SN, Makharita RR, et al. Detection of gyra and parc mutations and prevalence of plasmid-mediated quinolone resistance genes in Klebsiella pneumoniae. Infect Drug Resist 2021;14:555.
  7. Lagha R, Abdallah FB, ALKhammash AA, Amor N, Hassan MM, Mabrouk I, et al. Molecular characterization of multidrug resistant Klebsiella pneumoniae clinical isolates recovered from King Abdulaziz Specialist Hospital at Taif City, Saudi Arabia. J Infect Public Health. 2021;14(1):143-51.
  8. Li J, Huang Z-Y, Yu T, Tao X-Y, Hu Y-M, Wang H-C, et al. Isolation and characterization of a sequence type 25 carbapenem-resistant hypervirulent Klebsiella pneumoniae from the mid-south region of China. BMC Microbiol. 2019;19(1):1-10.
  9. Maleki D, Jahromy SH, Karizi SZ, Eslami P. The prevalence of acrA and acrB genes among multiple-drug resistant uropathogenic Escherichia coli isolated from patients with UTI in Milad Hospital, Tehran. Avicenna J Clin Microbiol Infect. 2016;4(1):39785-.
  10. Manohar P, Tamhankar AJ, Lundborg CS, Nachimuthu R. Therapeutic characterization and efficacy of bacteriophage cocktails infecting Escherichia coli, Klebsiella pneumoniae, and Enterobacter species. Front Microbiol. 2019;10:574.
  11. Martin RM, Bachman MA. Colonization, infection, and the accessory genome of Klebsiella pneumoniae. Front Cell Infect Microbiol. 2018;8:4.
  12. Padilla E, Llobet E, Doménech-Sánchez A, Martínez-Martínez L, Bengoechea JA, Albertí S. Klebsiella pneumoniae AcrAB efflux pump contributes to antimicrobial resistance and virulence. Antimicrob Agents Chemother. 2010;54(1):177-83.
  13. Jabar RMA, Hassoon AH. The expression of efflux pump AcrAB in MDR Klebsiella pneumoniae isolated from Iraqi patients. J Pharm Sci Res. 2019;11(2):423-8.
  14. Hinthong W, Pumipuntu N, Santajit S, Kulpeanprasit S, Buranasinsup S, Sookrung N, et al. Detection and drug resistance profile of Escherichia coli from subclinical mastitis cows and water supply in dairy farms in Saraburi Province, Thailand. PeerJ. 2017;5:e3431.
  15. Herran OH. Investigate resistance genes of antibacterials of the isolated bacteria from some clinical injuries in Diwaniyah. Iraq: University of Qadisiyah; 2014.
  16. Yousefi Mashouf R, Alijani P, Saidijam M, Alikhani MY, Rashidi H. Study of antibiotic resistance pattern and phenotypic detection of ESBLs in Klebsiella pneumoniae strains isolated from clinical samples and determination of minimum inhibitory concentrations of imipenem and ceftazidim antibiotics. Avicenna J Med Biotechnol. 2014;20(4):295-302.
  17. Abdu A, Kachallah M, Bolus DY. Antibiotic susceptibility patterns of Uropathogenic Escherichia coli among patients with urinary tract infections in a tertiary care hospital in Maiduguri, North Eastern, Nigeria. J Biosci Biotechnol Discov. 2018;3:14-24.
  18. Tajbakhsh E, Ahmadi P, Abedpour-Dehkordi E, Arbab-Soleimani N, Khamesipour F. Biofilm formation, antimicrobial susceptibility, serogroups and virulence genes of uropathogenic E. coli isolated from clinical samples in Iran. Antimicrob Resist Infect Control. 2016;5(1):1-8.
  19. Suresh M, Nithya N, Jayasree P, PR MK. Detection And Prevalence Of Efflux Pump-Mediated Drug Resistance In Clinical Isolates Of Multidrug-Resistant Gram-Negative Bacteria From North Kerala, India. Asian J Pharm Clin Res. 2016:324-7.
  20. Paltansing S. Antimicrobial resistance in Enterobacteriaceae: characterization and detection: Leiden University; 2015.
  21. Zaman SB, Hussain MA, Nye R, Mehta V, Mamun KT, Hossain N. A review on antibiotic resistance: alarm bells are ringing. Cureus. 2017;9(6).
  22. Kapoor G, Saigal S, Elongavan A. Action and resistance mechanisms of antibiotics: A guide for clinicians. J Anaesthesiol Clin Pharmacol. 2017;33(3):300.
  23. Kibret M, Abera B. Antimicrobial susceptibility patterns of E. coli from clinical sources in northeast Ethiopia. Afr Health Sci. 2011;11:40-5.
  24. Kafilzadeh F, Farsimadan F. Investigating multidrug efflux pumps in relation to the antibiotic resistance pattern in Escherichia coli strains from patients in Iran. Biomed Res. 2016;27(4):1130-5.
  1. Ogawa W, Onishi M, Ni R, Tsuchiya T, Kuroda T. Functional study of the novel multidrug efflux pump KexD from Klebsiella pneumoniae. Gene. 2012;498(2):177-82.
  2. Blair JM, Piddock LJ. Structure, function and inhibition of RND efflux pumps in Gram-negative bacteria: an update. Curr Opin Microbiol. 2009;12(5):512-9.
  3. Maina D, Makau P, Nyerere A, Revathi G. Antimicrobial resistance patterns in extended-spectrum β-lactamase producing Escherichia coli and Klebsiella pneumoniae isolates in a private tertiary hospital, Kenya. Microbiol Discov. 2013;1(5):1.
  4. Munoz-Davila MJ. Role of old antibiotics in the era of antibiotic resistance. Highlighted nitrofurantoin for the treatment of lower urinary tract infections. Antibiotics. 2014;3(1):39-48.
  5. Tsai Y-K, Fung C-P, Lin J-C, Chen J-H, Chang F-Y, Chen T-L, et al. Klebsiella pneumoniae outer membrane porins OmpK35 and OmpK36 play roles in both antimicrobial resistance and virulence. Antimicrob Agents Chemother. 2011;55(4):1485-93.
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