اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,073
تعداد مقالات34,300
تعداد مشاهده مقاله47,109,500
تعداد دریافت فایل اصل مقاله78,017,581
Hayder Hamad, M, Eidan Hadi, M, Ajam, I. K. (1401). Comparison between Polymerase Chain Reaction and Blood Culture for Diagnosis of Neonatal Sepsis. سامانه مدیریت نشریات علمی, 78(1), 221-226. doi: 10.22092/ari.2022.358608.2259
M Hayder Hamad; M Eidan Hadi; I. K Ajam. "Comparison between Polymerase Chain Reaction and Blood Culture for Diagnosis of Neonatal Sepsis". سامانه مدیریت نشریات علمی, 78, 1, 1401, 221-226. doi: 10.22092/ari.2022.358608.2259
Hayder Hamad, M, Eidan Hadi, M, Ajam, I. K. (1401). 'Comparison between Polymerase Chain Reaction and Blood Culture for Diagnosis of Neonatal Sepsis', سامانه مدیریت نشریات علمی, 78(1), pp. 221-226. doi: 10.22092/ari.2022.358608.2259
Hayder Hamad, M, Eidan Hadi, M, Ajam, I. K. Comparison between Polymerase Chain Reaction and Blood Culture for Diagnosis of Neonatal Sepsis. سامانه مدیریت نشریات علمی, 1401; 78(1): 221-226. doi: 10.22092/ari.2022.358608.2259

Comparison between Polymerase Chain Reaction and Blood Culture for Diagnosis of Neonatal Sepsis

Archives of Razi Institute
مقاله 29، دوره 78، شماره 1، فروردین و اردیبهشت 2023، صفحه 221-226    اصل مقاله (534.85 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.358608.2259
نویسندگان
M Hayder Hamad* 1؛ M Eidan Hadi1؛ I. K Ajam2
1Al Mustaqbal University College, Babylon, Iraq
2Islamic University, Babylon, Iraq
چکیده
Neonatal sepsis can be defined as any systemic bacterial infection confirmed by a positive blood culture in the first month of life. This study evaluated the polymerase chain reaction as the diagnostic approach to identify neonatal sepsis instead of blood culture.   In this study, 85 blood specimens were collected from 85 patients with suspected septicemia; ages ranged between 1 to 28 days from both sexes (53 males and 32 females) from November 2014 to March 2015. From each neonate, a minimum of 1–3 ml of blood was collected by standard sterile procedures, 2 ml for blood culture, while 1 ml was used for DNA extraction. A minimum of 2 ml of blood is taken through venipuncture and injected into two or more "blood bottles" with specific media for aerobic and anaerobic organisms. The blood is collected using an aseptic technique. The recorded data showed that the bacterial culture was positive in 7.06% of patients versus 92.9%, revealing a negative bacterial culture. The most common types of bacteria isolated were three isolates of Klebsiella spp. (50.0%), followed by one isolate of Staphylococcus aureus (16.67%), one E. coli (16.67%) isolate, and one Enterobacter spp. (16.67%) isolate. Finally, molecular detection for bacterial sepsis was done using specific primers (16 sRNA, rpoB and its). It was found that 16 sRNA genes were present in 20% of samples, and rpoB gene was present in (18.8%). While its gene used for the detection of fungi revealed negative results in all samples.
کلیدواژه‌ها
Neonatal sepsis؛ 16 sRNA؛ PCR؛ Septicemia
سایر فایل های مرتبط با مقاله
مراجع
  1. Fanaroff AA, Stoll BJ, Wright LL, Carlo WA, Ehrenkranz RA, Stark AR, et al. Trends in neonatal morbidity and mortality for very low birthweight infants. Am J Obstet Gynecol. 2007;196(2):147.
  2. Ottolini MC, Lundgren K, Mirkinson LJ, Cason S, Ottolini MG. Utility of complete blood count and blood culture screening to diagnose neonatal sepsis in the asymptomatic at risk newborn. Pediatr Infect Dis J. 2003;22(5):430-4.
  3. Shah AJ, Mulla SA, Revdiwala SB. Neonatal sepsis: high antibiotic resistance of the bacterial pathogens in a neonatal intensive care unit of a tertiary care hospital. J Clin Neonatol. 2012;1(2):72.
  4. Mutlu M, Aslan Y, Saygin B, Yilmaz G, Bayramoğlu G, Köksal I. Neonatal Sepsis Caused by Gram-negative Bacteria in a Neonatal Intensive Care Unit: A Six Years Analysis 新生兒深切治療部中由革蘭氏陰性細菌所導致的醫院內感染性膿毒癥: 一個持續六年的分析. HK J Paediatr (new series). 2011;16(4):253-7.
  5. Baltimore RS, Huie SM, Meek JI, Schuchat A, O'Brien KL. Early-onset neonatal sepsis in the era of group B streptococcal prevention. Pediatrics. 2001;108(5):1094-8.
  6. Verani JR, McGee L, Schrag SJ. Prevention of perinatal group B streptococcal disease: revised guidelines from CDC, 2010. 2010.
  7. Murty D, Gyaneshwari M. Blood cultures in paediatric patients: A study of clinical impact. Indian J Med Microbiol. 2007;25(3):220-4.
  8. Ishibashi M, Takemura Y, Ishida H, Watanabe K, Kawai T. C-reactive protein kinetics in newborns: application of a high-sensitivity analytic method in its determination. Clin Chem. 2002;48(7):1103-6.
  9. Aurangzeb B, Hameed A. Neonatal sepsis in hospital-born babies: bacterial isolates and antibiotic susceptibility patterns. J Coll Physicians Surg Pak. 2003;13:629-32.
  10. Agnihotri N, Kaistha N, Gupta V. Antimicrobial susceptibility of isolates from neonatal septicemia. Jpn J Infect Dis. 2004;57(6):273-5.
  11. Haque S, Jahan N, Mannan M, Hasan M, Begum M, Rob S, et al. Identification of bacterial isolates in neonatal sepsis and their antimicrobial susceptibility. Mymensingh Med J. 2014;23(4):709-14.
  1. Ahmed AS, Chowdhury MA, Hoque M, Darmstadt GL. Clinical and bacteriological profile of neonatal septicemia in tertiary level pediatric hospital in Bangladesh. Indian Pediatr. 2002;39(11):1034-9.
  2. Nathoo K, Mason P, Gwanzura L, Kowo H, Mubaiwa L. Severe Klebsiella infection as a cause of mortality in neonates in Harare, Zimbabwe: evidence from postmortem blood cultures. Pediatr Infect Dis J. 1993;12(10):840-4.
  3. Aftab R, Iqbal I. Bacteriological agents of neonatal sepsis in NICU at Nishtar Hospital Multan. J Coll Physicians Surg Pak. 2006;16(3):216-9.
  4. Kellogg JA, Ferrentino FL, Goodstein MH, Liss J, Shapiro SL, Bankert DA. Frequency of low level bacteremia in infants from birth to two months of age. Pediatr Infect Dis J. 1997;16(4):381-5.
  5. Ahmed Z, Ghafoor T, Waqar T, Ali S, Aziz S, Mahmud S. Diagnostic value of C-reactive protein and haematological parameters in neonatal sepsis. J Coll Physicians Surg Pak. 2005;15(3):152-6.
  6. Mancini N, Carletti S, Ghidoli N, Cichero P, Burioni R, Clementi M. The era of molecular and other non-culture-based methods in diagnosis of sepsis. Clin Microbiol Rev. 2010;23(1):235-51.
  7. Elamreen FA. Neonatal sepsis due to multidrug-resistant Klebsiella terrigena in the neonatal intensive care unit in Gaza, Palestine. Crit Care. 2007;11(4):1-.
  8. Chiesa C, Panero A, Osborn JF, Simonetti AF, Pacifico L. Diagnosis of neonatal sepsis: a clinical and laboratory challenge. Clin Chem. 2004;50(2):279-87.
  1. Kapoor L, Randhawa V, Deb M. Microbiological profile of neonatal septicemia in a pediatric care hospital in Delhi. J Commun Dis. 2005;37(3):227-32.
  2. Afroza S. Neonatal sepsis--a global problem: an overview. Mymensingh Med J. 2006;15(1):108-14.
  3. Shang S, Chen G, Wu Y, Du L, Zhao Z. Rapid diagnosis of bacterial sepsis with PCR amplification and microarray hybridization in 16S rRNA gene. Pediatr Res. 2005;58(1):143-8.
  4. Schuchat A, Whitney CG, Zangwill K. Prevention of perinatal group B streptococcal disease: a public health perspective. 1996.
  5. Chen Y-C, Eisner JD, Kattar MM, Rassoulian-Barrett SL, Lafe K, Bui U, et al. Polymorphic internal transcribed spacer region 1 DNA sequences identify medically important yeasts. J Clin Microbiol. 2001;39(11):4042-51.
  6. Bindayna KM, Jamsheer A, Farid E, Botta GA. Neonatal Sepsis 1991–2001: Prevalent bacterial agents and antimicrobial susceptibilities in Bahrain. Med Princ Pract. 2006;15(2):131-6.
  7. Brown DR, Kutler D, Rai B, Chan T, Cohen M. Bacterial concentration and blood volume required for a positive blood culture. J Perinatol. 1995;15(2):157-9.
  8. Ahmad S, Khan Z, Mustafa AS, Khan ZU. Seminested PCR for diagnosis of candidemia: comparison with culture, antigen detection, and biochemical methods for species identification. J Clin Microbiol. 2002;40(7):2483-9.
  9. Fazzeli H, Arabestani MR, Esfahani BN, Khorvash F, Pourshafie MR, Moghim S, et al. Development of PCR-based method for detection of Enterobacteriaceae in septicemia. J Res Med Sci. 2012;17(7):671.
آمار
تعداد مشاهده مقاله: 3,161
تعداد دریافت فایل اصل مقاله: 6,538


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب