اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,074
تعداد مقالات34,301
تعداد مشاهده مقاله47,138,848
تعداد دریافت فایل اصل مقاله78,044,457
Najd Obaid, F, Al-Dabbagh, K. A, Salam Karim, Y, Abdallah Mahdi, O, Mahdi Rheima, A, Alkadir, O. K. A., Hamad, D. A, Mahmood Shihab, E, Hussin Alwan, N, Alwaily, E. R. (1401). Presence of Escherichia coli O157:H7 in Dairy Farms located in Najaf, Baghdad, Kirkuk, and Erbil, Iraq. سامانه مدیریت نشریات علمی, 77(3), 1269-1273. doi: 10.22092/ari.2022.358832.2317
F Najd Obaid; K. A Al-Dabbagh; Y Salam Karim; O Abdallah Mahdi; A Mahdi Rheima; O. K. A. Alkadir; D. A Hamad; E Mahmood Shihab; N Hussin Alwan; E. R Alwaily. "Presence of Escherichia coli O157:H7 in Dairy Farms located in Najaf, Baghdad, Kirkuk, and Erbil, Iraq". سامانه مدیریت نشریات علمی, 77, 3, 1401, 1269-1273. doi: 10.22092/ari.2022.358832.2317
Najd Obaid, F, Al-Dabbagh, K. A, Salam Karim, Y, Abdallah Mahdi, O, Mahdi Rheima, A, Alkadir, O. K. A., Hamad, D. A, Mahmood Shihab, E, Hussin Alwan, N, Alwaily, E. R. (1401). 'Presence of Escherichia coli O157:H7 in Dairy Farms located in Najaf, Baghdad, Kirkuk, and Erbil, Iraq', سامانه مدیریت نشریات علمی, 77(3), pp. 1269-1273. doi: 10.22092/ari.2022.358832.2317
Najd Obaid, F, Al-Dabbagh, K. A, Salam Karim, Y, Abdallah Mahdi, O, Mahdi Rheima, A, Alkadir, O. K. A., Hamad, D. A, Mahmood Shihab, E, Hussin Alwan, N, Alwaily, E. R. Presence of Escherichia coli O157:H7 in Dairy Farms located in Najaf, Baghdad, Kirkuk, and Erbil, Iraq. سامانه مدیریت نشریات علمی, 1401; 77(3): 1269-1273. doi: 10.22092/ari.2022.358832.2317

Presence of Escherichia coli O157:H7 in Dairy Farms located in Najaf, Baghdad, Kirkuk, and Erbil, Iraq

Archives of Razi Institute
مقاله 45، دوره 77، شماره 3، مرداد و شهریور 2022، صفحه 1269-1273    اصل مقاله (426.56 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.358832.2317
نویسندگان
F Najd Obaid1؛ K. A Al-Dabbagh2؛ Y Salam Karim3؛ O Abdallah Mahdi4؛ A Mahdi Rheima5، 6؛ O. K. A. Alkadir7؛ D. A Hamad8؛ E Mahmood Shihab9؛ N Hussin Alwan10؛ E. R Alwaily* 11
1Altoosi University College, Najaf, Iraq
2Department of Dentistry, Al-Hadba University College, Mosul, Iraq
3Department of Pharmacy, Al-Manara College For Medical Sciences, Maysan, Iraq
4Anesthesia Techniques Department, Al-Mustaqbal University College, Babylon, Iraq
5College of Technical Engineering, The Islamic University, Najaf, Iraq
6Department of Optics Techniques, Dijlah University College, Al-Masafi Street, Al-Dora, Baghdad 00964, Iraq
7Al-Nisour University College, Baghdad, Iraq
8Nursing Department, Hilla University College, Babylon, Iraq
9Al-Esraa University College, Baghdad, Iraq
10Department of Nursing, Al-Zahrawi University College, Karbala, Iraq
11Microbiology Research Group , College of Pharmacy, Al-Ayen University, Thi-Qar, Iraq
چکیده
It has been approved that one of the most dangerous foodborne pathogenic bacteria is E. coli O157:H7, which is responsible for several infection and death cases worldwide. It is well documented that in the developing countries E. coli O157:H7 is considered the main causative pathogen of human gastrointestinal infections. Therefore, the current research was aimed to evaluate the prevalence of E. coli O157:H7 in dairy cattle's milk using a rapid method, in Iraq (Najaf, Baghdad, Kirkuk, and Erbil). Over a period of 6 months (During hot months) samples were obtained and investigated by culturing on selective media (CT-SMAC).  The multiplex PCR (m-PCR) also used for milk sample direct investigation. Using biochemical tests the recorded data showed that, 2 recognized isolates were E. coli, while the recorded data obtained from m-PCR assay revealed that none of the isolated E. coli was toxigenic E.coli O157:H7. The results of m-PCR on the milk samples revealed that 45 milk samples contained at least one of the following genes: O157, H7, stx1, stx2 genes. Also the results of the m-PCR revealed that 2 samples (raw milk) were toxigenic O157:H7 positive. In conclusion, to the best of authors' knowledge, this investigation was the first report on the prevalence of E. coli O157:H7 in the raw milk samples in Iraq. The results showed that the proportion of contaminated milk samples contaminated with E. coli O157:H7 identified in the current survey were similar to that the results of the previously published research from different dairy products across different countries in the Middle East region.
کلیدواژه‌ها
Milk؛ Escherichia coli O157:H7؛ polymerase chain reaction
مراجع
  1. Gilbert C, Winters D, O'Leary A, Slavik M. Development of a triplex PCR assay for the specific detection of Campylobacter jejuni, Salmonella spp., and Escherichia coli O157: H7. Mol Cell Probes. 2003;17(4):135-8.
  2. Li B, Liu H, Wang W. Multiplex real-time PCR assay for detection of Escherichia coli O157:H7 and screening for non-O157 Shiga toxin-producing E. coli. BMC Microb. 2017;17(1):215.
  3. Excler J-L, Saville M, Berkley S, Kim JH. Vaccine development for emerging infectious diseases. Nat Med. 2021;27(4):591-600.
  4. Law D. Virulence factors of Escherichia coli O157 and other Shiga toxin‐producing E. coli. J Appl Microbiol. 2000;88(5):729-45.
  5. Franzin FM, Sircili MP. Locus of enterocyte effacement: a pathogenicity island involved in the virulence of enteropathogenic and enterohemorragic Escherichia coli subjected to a complex network of gene regulation. Biomed Res Int. 2015;2015.
  6. Clarke S, Haigh R, Freestone P, Williams P. Virulence of enteropathogenic Escherichia coli, a global pathogen. Clin Microbiol Rev. 2003;16(3):365-78.
  7. Elliott SJ, Wainwright LA, McDaniel TK, Jarvis KG, Deng Y, Lai LC, et al. The complete sequence of the locus of enterocyte effacement (LEE) from enteropathogenic Escherichia coli E2348/69. Mol Microb. 1998;28(1):1-4.
  8. Hancock DD, Besser TE, Rice DH, Ebel ED, Herriott DE, Carpenter LV. Multiple sources of Escherichia coli O157 in feedlots and dairy farms in the northwestern USA. Prev Vet Med. 1998;35(1):11-9.
  9. Bai J, Shi X, Nagaraja T. A multiplex PCR procedure for the detection of six major virulence genes in Escherichia coli O157: H7. J Microbiol Methods. 2010;82(1):85-9.
  10. Solomakos N, Govaris A, Angelidis AS, Pournaras S, Burriel AR, Kritas SK, et al. Occurrence, virulence genes and antibiotic resistance of Escherichia coli O157 isolated from raw bovine, caprine and ovine milk in Greece. Food Microbiol. 2009;26(8):865-71.
  1. Desmarchelier PM, Bilge SS, Fegan N, Mills L, Vary JC, Jr., Tarr PI. A PCR specific for Escherichia coli O157 based on the rfb locus encoding O157 lipopolysaccharide. J Clin Microbiol. 1998;36(6):1801-4.
  2. Paton JC, Paton AW. Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections. Clin Microbiol Rev. 1998;11(3):450-79.
  3. Karch H, Bielaszewska M, Bitzan M, Schmidt H. Epidemiology and diagnosis of Shiga toxin-producing Escherichia coli infections. Diagn Microbiol Infect Dis. 1999;34(3):229-43.
  4. Meshref AMS. Bacteriological quality and safety of raw cow’s milk and fresh cream. Slov Vet Zb. 2013;50(1):21-30.
  5. Blanco J, Blanco M, Blanco JE, Mora A, Alonso MP, Gonzalez EA, et al. Epidemiology of verocytotoxigenic Escherichia coli (VTEC) in ruminants. Verocytotoxigenic E coli. 2001;113.
  6. Rahimi E, Kazemeini HR, Salajegheh M, editors. Escherichia coli O157: H7/NM prevalence in raw beef, camel, sheep, goat, and water buffalo meat in Fars and Khuzestan provinces, Iran. Veterinary Research Forum; 2012: Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
  7. Byrne C, Erol I, Call J, Kaspar C, Buege D, Hiemke C, et al. Characterization of Escherichia coli O157: H7 from downer and healthy dairy cattle in the upper Midwest region of the United States. Appl Environ Microbiol. 2003;69(8):4683-8.
  8. Elder RO, Keen JE, Siragusa GR, Barkocy-Gallagher GA, Koohmaraie M, Laegreid WW. Correlation of enterohemorrhagic Escherichia coli O157 prevalence in feces, hides, and carcasses of beef cattle during processing. Proc Natl Acad Sc. 2000;97(7):2999-3003.
  9. Cagney C, Crowley H, Duffy G, Sheridan J, O’brien S, Carney E, et al. Prevalence and numbers of Escherichia coli O157: H7 in minced beef and beef burgers from butcher shops and supermarkets in the Republic of Ireland. Food Microbiol. 2004;21(2):203-12.
آمار
تعداد مشاهده مقاله: 1,856
تعداد دریافت فایل اصل مقاله: 2,737


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب