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اثر مراحل مختلف فنولوژی بر پروفایل شیمیایی و خواص آنتی‌اکسیدانی، ضد باکتریایی و ضد التهابی عصاره‌های ریشه و اندام‌های هوایی Artemisia absinthium L. | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 4، دوره 38، شماره 3 - شماره پیاپی 113، مهر 1401، صفحه 390-408 اصل مقاله (1 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2022.358105.3148 | ||
| نویسندگان | ||
| محمود براتی1؛ مجید شریفی راد* 2؛ سعیده سعیدی3 | ||
| 1گروه مرتع و آبخیزداری دانشگاه زابل | ||
| 2گروه مرتع و آبخیزداری، دانشکده آب و خاک، دانشگاه زابل | ||
| 3دانشگاه زابل | ||
| چکیده | ||
| برای بررسی پروفایل شیمیایی و خواص آنتی‌اکسیدانی، ضد باکتریایی و ضد التهابی ریشه و اندام‌های هوایی گونه مرتعی- دارویی افسنطین (Artemisia absinthium L.)، نمونهبرداری از ریشه و اندام‌های هوایی گیاه در سه مرحله فنولوژیک (رشد رویشی، گلدهی و بذردهی) انجام شد. میزان فنل و فلاونوئید، فعالیت زیستی عصاره اتانولی در مراحل مختلف فنولوژی، فعالیت آنتی‌اکسیدانی، قدرت احیاءکنندگی آهن (FRAP)، فعالیت ضد باکتریایی، حداقل غلظت مهارکنندگی (MIC) و حداقل غلظت کشندگی (MBC) و فعالیت ضد التهابی بررسی شدند. بیشترین میزان فنل کل (2 ± 4/86 میلی‌گرم برابر اسید گالیک بر گرم وزن خشک گیاه) و فلاونوئید کل (3/1 ± 36.2 میلی‌گرم برابر کوئرستین بر گرم وزن خشک گیاه) در ریشه و در مرحله گلدهی گیاه وجود داشت. بررسی فعالیت آنتی‌اکسیدانی عصاره‌ها نشان داد که مرحله گلدهی گیاه از فعالیت آنتی‌اکسیدانی بالاتری برخوردار بود. حداقل مقدار IC50 (15.4 میکروگرم بر میلی‌لیتر) مربوط به عصاره ریشه در مرحله گلدهی گیاه بود و بالاترین میزان IC50 (271.65 میکروگرم بر میلی‌لیتر) برای عصاره اندام‌های هوایی در مرحله رویشی بود. در بررسی فعالیت آنتی‌باکتریایی عصاره‌ها، عصاره ریشه مربوط به مرحله گلدهی گیاه از بالاترین فعالیت ضد باکتریایی برخوردار بود و بیشترین قطر هاله عدم رشد (22 میلی‌متر) برای این عصاره در برابر باکتری استافیلوکوکوس اورئوس مشاهده گردید. حداقل غلظت مهار (18.7 میلی‌گرم بر میلی‌لیتر) و حداقل غلظت کشندگی (37.5 میلی‌گرم بر میلی‌لیتر) این عصاره در برابر باکتری‌های استافیلوکوکوس اورئوس و باسیلوس سرئوس ثبت شد. باکتری‌های ویبریوکلرا (حداقل غلظت مهار 37.5 میلی‌گرم بر میلی‌لیتر و حداقل غلظت کشندگی 75 میلی‌گرم بر میلی‌لیتر) و اشریشیاکلی (حداقل غلظت کشندگی 75 میلی‌گرم بر میلی‌لیتر و حداقل غلظت کشندگی 150 میلی‌گرم بر میلی‌لیتر) در برابر عصاره مذکور حساسیت کمتری نشان دادند. همچنین نتایج نشان داد که گونه مورد مطالعه در مرحله گلدهی دارای بیشترین فعالیت ضد التهابی است و عصاره بدست آمده از ریشه گیاه در تمام مراحل فنولوژی از فعالیت ضد التهابی بالاتری نسبت به اندام‌های هوایی گیاه برخوردار است. براساس این نتایج، گیاه افسنطین و به‌طور خاص مرحله گلدهی آن دارای فعالیت آنتی‌اکسیدانی، ضد باکتریایی و ضد التهابی مناسبی است و می‌توان آن را به‌عنوان جایگزینی مناسب برای آنتی‌اکسیدان‌ها و آنتی‌بیوتیک‌ها و ضد التهاب‌های مصنوعی مد نظر قرار داد. | ||
| کلیدواژه‌ها | ||
| ترکیب‌های شیمیایی؛ فعالیت آنتی‌اکسیدانی؛ فعالیت ضد باکتریایی؛ فعالیت ضد التهابی؛ مرحله فنولوژی؛ Artemisia absinthium L | ||
| عنوان مقاله [English] | ||
| Effects of different phenological stages on chemical profile, antioxidant, antibacterial, and anti-inflammatory activities of Artemisia absinthium L. root and aerial parts extracts | ||
| نویسندگان [English] | ||
| Mahmood Barati1؛ Majid Sharifi-Rad2؛ Saeede saeedi3 | ||
| 1Department of Range and watershed management, university of zabol | ||
| 2Department of Range and Watershed Management, University of Zabol | ||
| 3university of Zabol | ||
| چکیده [English] | ||
| Due to the importance of rangeland medicinal plants, the present study was conducted to assess the chemical profile and antioxidant, antibacterial, and anti-inflammatory activities of rangeland-medicinal plant Artemisia absinthium L. root and aerial parts extracts at different phenological stages (vegetative, flowering, and seeding). Folin-Ciocalteu and aluminum chloride colorimetric methods were used for determination of total phenol and flavonoids contents, respectively. The ethanol extract biological activities were investigated at different phenological stages: the antioxidant activity by DPPH free radical scavenging activity and ferric reducing antioxidant power (FRAP) methods; the antibacterial activity by disk diffusion, minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods; and the anti-inflammatory activity using human red blood cell stabilization. The results showed that the highest amount of total phenol (86.4 ± 2 mg gallic acid equivalents (GAE)/g dry weight) and total flavonoids (36.2 ± 1.3 mg quercetin equivalents (QE)/g dry weight) was recorded for the root at flowering stage. Also, the root extract at flowering stage had the highest antioxidant (IC50 = 15.4 μg.mL-1) and antibacterial activities. The largest inhibition zone (22 mm) was observed for this extract against Staphylococcus aureus. This extract MIC and MBC were recorded 18.7 mg.mL-1 and 37.5 mg.mL-1 against S. aureus and Bacillus cereus, respectively. The bacteria Vibrio cholerae and Escherichia coli showed less sensitivity to the mentioned extract. Also, the results showed that the flowering stage had the highest anti-inflammatory activity compared to the other phenological stages. The root extract at the all phenological stages showed higher anti-inflammatory activity than the aerial parts. Overall, it could be concluded that A. absinthium, particularly at flowering stage, can be considered as a suitable alternative source for synthetic antioxidants, antibiotics, and anti-inflammatory agents. | ||
| کلیدواژه‌ها [English] | ||
| Phytochemical compounds, antioxidant activity, antibacterial activity, anti-inflammatory activity, phenological stages, Artemisia absinthium L | ||
| مراجع | ||
|
- Ahamad, J., 2019. A pharmacognostic review on Artemisia absinthium. International Research Journal of Pharmacy, 10(1): 25-31. - Aryal, S., Baniya, M.K., Danekhu, K., Kunwar, P., Gurung, R. and Koirala, N., 2019. Total phenolic content, flavonoid content and antioxidant potential of wild vegetables from Western Nepal. Plants, 8: 96. - Bag, A., Kumar Bhattacharyya, S., Kumar Pal, N. and Ranjan Chattopadhyay, R., 2013. Anti-inflammatory, anti-lipid peroxidative, antioxidant and membrane stabilizing activities of hydroalcoholic extract of Terminalia chebula fruits. Pharmaceutical Biology, 51: 1515-1520. - Batiha, G.E.S., Olatunde, A., El-Mleeh, A., Hetta, H.F., Al-Rejaie, S., Alghamdi, S., Zahoor, M., Magdy Beshbishy, A., Murata, T., Zaragoza-Bastida, A. and Rivero-Perez, N., 2020. Bioactive compounds, pharmacological actions, and pharmacokinetics of wormwood (Artemisia absinthium). Antibiotics, 9(6): 353. - Benzie, I.F. and Strain, J.J. 1996. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry, 239(1): 70-76. - Bhat, R.R., Rehman, M.U., Shabir, A., Rahman Mir, M.U., Ahmad, A., Khan, R., Masoodi, M.H., Madkhali, H. and Ganaie, M.A., 2019. Chemical composition and biological uses of Artemisia absinthium (wormwood). Plant and Human Health, 3: 37-63. - Chang, C., Yang, M., Wen, H. and Chern, J., 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10: 178-182. - Das, K., Tiwari, R.K.S. and Shrivastava, D.K. 2010. Techniques for evaluation of medicinal plant products as antimicrobial agent: Current methods and future trends. Journal of Medicinal Plants Research, 4(2): 104-111. - Emami, S.A., Rabe, S.Z.T., Ahi, A. and Mahmoudi, M., 2012. Inhibitory activity of eleven Artemisia species from Iran against Leishmania major parasites. Iranian Journal of Basic Medical Sciences, 15(2): 807. - Farghadan, M., Ghafoori, H., Vakhshiteh, F., Fazeli, S.A.S., Farzaneh, P. and Kokhaei, P., 2016. The Effect of Artemisia fragrans Willd: essential oil on inducible nitric oxide synthase gene expression and nitric oxide production in lipopolysaccharide-stimulated murine macrophage cell line. Iranian Journal of Allergy, Asthma and Immunology, - Feduraev, P., Chupakhina, G., Maslennikov, P., Tacenko, N. and Skrypnik, L., 2019. Variation in phenolic compounds content and antioxidant activity of different plant organs from Rumex crispus L. and Rumex obtusifolius L. at different growth stages. Antioxidants, 8(7): 237. - Ghasemzadeh, A., Jaafar, H.Z., Ashkani, S., Rahmat, A., Juraimi, A.S., Puteh, A. and Muda Mohamed, M.T., 2016. Variation in secondary metabolite production as well as antioxidant and antibacterial activities of Zingiber zerumbet (L.) at different stages of growth. BMC Complementary and Alternative Medicine, 16(1): 1-10. - Greten, F.R. and Grivennikov, S.I., 2019. Inflammation and cancer: triggers, mechanisms, and consequences. Immunity, 51(1): 27-41. - Hbika, A., Daoudi, N.E., Bouyanzer, A., Bouhrim, M., Mohti, H., Loukili, E.H., Mechchate, H., Al-Salahi, R., Nasr, F.A., Bnouham, M. and Zaid, A., 2022. Artemisia absinthium L. aqueous and ethyl acetate extracts: antioxidant effect and potential activity in vitro and in vivo against pancreatic α-amylase and intestinal α-glucosidase. Pharmaceutics, 14(3): 481. - Heimer, L. and Alheid, G.F., 1991. Piecing together the puzzle of basal forebrain anatomy. Advancesin Experimental Medicine and Biology, 295: 1-42. - Ivanov, M., Gašić, U., Stojković, D., Kostić, M., Mišić, D. and Soković, M., 2021. New Evidence for Artemisia absinthium L. application in gastrointestinal ailments: ethnopharmacology, antimicrobial capacity, cytotoxicity, and phenolic profile. Evidence-Based Complementary and Alternative Medicine, 2021: 321-336. - Jardim, M.C. 2005. Role of glutamate ionotropic and benzodiazepine receptors in the ventromedial hypothalamic nucleus on anxiety. Pharmacology Biochemistry and Behavior, 82(1): 182-189. - Ji, H.F., Li, X.J. and Zhang, H.Y., 2009. Natural products and drug discovery: can thousands of years of ancient medical knowledge lead us to new and powerful drug combinations in the fight against cancer and dementia? EMBO reports, 10(3): 194-200. - Jiang, C., Zhou, S., Liu, L., Toshmatov, Z., Huang, L., Shi, K., Zhang, C. and Shao, H., 2021. Evaluation of the phytotoxic effect of the essential oil from Artemisia absinthium. Ecotoxicology and Environmental Safety, 226: 112856. - Jimoh, M.O., Afolayan, A.J. and Lewu, F.B., 2019. Antioxidant and phytochemical activities of Amaranthus caudatus L. harvested from different soils at various growth stages. Scientific Reports, 9: 12965. - Joshi, B. and Lekhak, S., 2009: Antibacterial property of different medicinal plants. Journal of Science Engineering and Technology, 5: 143-150. - Kabera, J.N., Semana, E., Mussa, A.R. and He, X., 2014. Plant secondary metabolites: biosynthesis, classification, function and pharmacological properties. Journal of Pharmacy and Pharmacology, 2(7): 377-392. - Kennedy, D.O. and Wightman, E.L., 2011. Herbal extracts and phytochemicals: plant secondary metabolites and the enhancement of human brain function. Advances in Nutrition, 2(1): 32-50. - Kordali, S., Kotan, R., Mavi, A., Cakir, A., Ala, A. and Yildirim, A., 2005. Determination of the chemical composition and antioxidant activity of the essential oil of Artemisia dracunculus and of the antifungal and antibacterial activities of Turkish Artemisia absinthium, A. dracunculus, Artemisia santonicum, and Artemisia spicigera essential oils. Journal of Agricultural and Food Chemistry, 53(24): 9452-9458. - Maina, S., Ryu, D.H., Bakari, G., Misinzo, G., Nho, C.W. and Kim, H.Y., 2021. Variation in phenolic compounds and antioxidant activity of various organs of African Cabbage (Cleome gynandra L.) accessions at different growth stages. Antioxidants, 10(12): 1952. - Mazutti, M., Mossi, A.J., Cansian, R.L., Corazza, M.L., Dariva, C. and Oliveira, J.V., 2008. Chemical profile and antimicrobial activity of Boldo (Peumus boldus Molina) extracts obtained by compressed carbon dioxide extraction. Brazilian Journal of Chemical Engineering, 25(2): 427-434. - Mckeegan, K.S., Borges Walmsley, M.I. and Walmsley, A.R., 2002. Microbial and viral drug resistance mechanisms. Trends in Microbiology,10(10): S8-S14. - Meda, A., Lamien, C.E., Romito, M., Millogo, J. and Nacoulma, O.G., 2005. Determination of the total phenolic, flavonoid and pralin contents in Burkina Fasan honey, as well as their scavenging activity. Food Chemistry, 91: 571-577. - Mohammadi, A., Sani, T.A., Ameri, A.A., Imani, M., Golmakani, E. and Kamali, H., 2015. Seasonal variation in the chemical composition, antioxidant activity, and total phenolic content of Artemisia absinthium essential oils. Pharmacognosy Research, 7(4): 329. - Mohammed, H.A., 2022. Phytochemical analysis, antioxidant potential, and cytotoxicity evaluation of traditionally used Artemisia absinthium L. (Wormwood) growing in the central region of Saudi Arabia. Plants, 11(8): 1028. - Mravčáková, D., Sobczak-Filipiak, M., Váradyová, Z., Kucková, K., Čobanová, K., Maršík, P., Tauchen, J., Vadlejch, J., Mickiewicz, M., Kaba, J. and Várady, M., 2021. Effect of Artemisia absinthium and Malva sylvestris on antioxidant parameters and abomasal histopathology in lambs experimentally infected with Haemonchus contortus. Animals, 11(2): 462. - Nayan, R.B. and Shukla, V.J., 2011., Antibacterial and antifungal activities from leaf extracts of Cassia fistula L.: An ethnomedicinal plant. Journal of Advanced Pharmaceutical Technology and Research, 2(2): 104-109. - Ncube, B. and Van Staden, J., 2015. Tilting plant metabolism for improved metabolite biosynthesis and enhanced human benefit. Molecules, 20: 12698-12731. - Nunes, C.D.R., Barreto Arantes, M., Menezes de Faria Pereira, S., Leandro da Cruz, L., de Souza Passos, M., Pereira de Moraes, L., Vieira, I.J.C. and Barros de Oliveira, D., 2020. Plants as sources of anti-inflammatory agents. Molecules, 25(16): 3726. - Oke, F., Aslim, B., Ozturk, S. and Altundag, S., 2009. Essential oil composition, antimicrobial and antioxidant activities of Satureja cuneifolia Ten. Food Chemistry, 112(4): 874-879. - Okoh, O., Sadimenko, A. and Afolayan, A., 2010. Comparative evaluation of the antibacterial activities of the essential oils of Rosmarinus officinalis L. obtained by hydrodistillation and solvent free microwave extraction methods. Food Chemistry, 120: 308-312. - Omer, B., Krebs, S., Omer, H. and Noor, T.O., 2007. Steroid-sparing effect of wormwood (Artemisia absinthium) in Crohn's disease: a double-blind placebo-controlled study. Phytomedicine, 14(2-3): 87-95. - Padosch, S.A., Lachenmeier, D.W. and Kröner, L.U., 2006. Absinthism: a fictitious 19th century syndrome with present impact. Substance Abuse Treatment, Prevention, and Policy, 1: 14. - Ranković, B.R., Kosanić, M.M. and Stanojković, T.P., 2011. Antioxidant, antimicrobial and anticancer activity of the lichens Cladonia furcata, Lecanora atra and Lecanora muralis. BMC Complementary and Alternative Medicine, 11(1): 1-8. - Ribeiro, D.A., de Macêdo, D.G., Boligon, A.A., Menezes, I.R.A., de Almeida Souza, M.M. and da Costa, J.G.M., 2019. Influence of seasonality on the phenolic composition of Secondatia floribunda A. DC (Apocynaceae) during its phenological cycle. Acta Physiologiae Plantarum, 41(12): 1-16. - Riipi, M., Ossipov, V., Lempa, K., Haukioja, E., Koricheva, J., Ossipova, S. and Pihlaja, K., 2002. Seasonal changes in birch leaf chemistry: Are there trade-offs between leaf growth and accumulation of phenolics? Oecologia, 130: 380-390. - Rock, K.L. and Kono, H., 2008. The inflammatory response to cell death. Annual Review of Pathology: Mechanisms of Disease, 3: 99-126. - Routray, W. and Orsat, V., 2014. Variation of phenolic profile and antioxidant activity of North American highbush blueberry leaves with variation of time of harvest and cultivar. Industrial Crops and Products, 62: 147-155. - Rustaiyan, A. and Masoudi, S., 2011. Chemical constituents and biological activities of Iranian Artemisia species. Phytochemistry Letters, 4(4): 440-447. - Samanta, A., Das, G. and Das, S.K., 2011 Roles of flavonoids in plants. Carbon, 100: 12-35. - Sen, A. and Batra, A., 2012. Evaluation of antimicrobial activity of different solvent extracts of medicinal plant: Melia azedarach L. International Journal of Current Pharmaceutical Research, - Shailesh, G., Seema, K. and Dwivedi, S., 2011. - Sofowora, A., Ogunbodede, E. and Onayade, A., 2013. The role and place of medicinal plants in the strategies for disease prevention. African Journal of Traditional, Complementary and Alternative Medicines, 10(5): 210-229. - Sultan, M.H., Zuwaiel, A.A., Moni, S.S., Alshahrani, S., Alqahtani, S.S., Madkhali, O. and Elmobark, M.E., 2020. Bioactive principles and potentiality of hot methanolic extract of the leaves from Artemisia absinthium L. “in vitro cytotoxicity against human MCF-7 breast cancer cells, antibacterial study and wound healing activity”. Current Pharmaceutical Biotechnology, 21(15): 1711-1721. - Szopa, A., Pajor, J., Klin, P., Rzepiela, A., Elansary, H.O., Al-Mana, F.A., Mattar, M.A. and Ekiert, H., 2020. Artemisia absinthium L. -Importance in the history of medicine, the latest advances in phytochemistry and therapeutical, cosmetological and culinary uses. Plants, 9(9): 1063. - Tungmunnithum, D., Thongboonyou, A., Pholboon, A. and Yangsabai, A., 2018. Flavonoids and other phenolic compounds from medicinal plants for pharmaceutical and medical aspects: An overview. Medicines, 5: 93. - Vane, J.R. and Botting, R.M. 1995. New insights into the mode of action of anti-inflammatory drugs. Inflammation Research, 44(1): 1-10. - Wang, J., 2018. Neutrophils in tissue injury and repair. Cell and Tissue Research, 371(3): 531-539. - Weli, A.M., Al-Salmi, S., Al Hoqani, H. and Hossain, M.A., 2018. Biological and phytochemical studies of different leaves extracts of Pteropyrum scoparium. Beni-Suef University Journal of Basic and Applied Sciences, 7(4): 481-486. - Yao, X.H., Zhang, Z.B., Song, P., Hao, J.Y., Zhang, D.Y. and Zhang, Y.F., 2016. Different harvest seasons modify bioactive compounds and antioxidant activities of Pyrola incarnata. Industrial Crops and Products, 94: 405-412. - Yineger, H., Kelbessa, E., Bekele, T. and Lulekal, E., 2007. Ethnoveterinary medicinal plants at bale mountains national park, Ethiopia. Journal of Ethnopharmacology, 112(1): 55-70. | ||
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