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Majid Jameel, Y, Kassem Khalil, Z. (1401). Relation between Serological Findings and Expression of IL-1β and IL-6 genes in Patients Infected with Mycobacterium Tuberculosis in Iraq. سامانه مدیریت نشریات علمی, 77(4), 1497-1502. doi: 10.22092/ari.2022.357620.2073
Y Majid Jameel; Z Kassem Khalil. "Relation between Serological Findings and Expression of IL-1β and IL-6 genes in Patients Infected with Mycobacterium Tuberculosis in Iraq". سامانه مدیریت نشریات علمی, 77, 4, 1401, 1497-1502. doi: 10.22092/ari.2022.357620.2073
Majid Jameel, Y, Kassem Khalil, Z. (1401). 'Relation between Serological Findings and Expression of IL-1β and IL-6 genes in Patients Infected with Mycobacterium Tuberculosis in Iraq', سامانه مدیریت نشریات علمی, 77(4), pp. 1497-1502. doi: 10.22092/ari.2022.357620.2073
Majid Jameel, Y, Kassem Khalil, Z. Relation between Serological Findings and Expression of IL-1β and IL-6 genes in Patients Infected with Mycobacterium Tuberculosis in Iraq. سامانه مدیریت نشریات علمی, 1401; 77(4): 1497-1502. doi: 10.22092/ari.2022.357620.2073

Relation between Serological Findings and Expression of IL-1β and IL-6 genes in Patients Infected with Mycobacterium Tuberculosis in Iraq

Archives of Razi Institute
مقاله 25، دوره 77، شماره 4، مهر و آبان 2022، صفحه 1497-1502    اصل مقاله (497.59 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.357620.2073
نویسندگان
Y Majid Jameel* ؛ Z Kassem Khalil
Department of Optometry, Medical-Technical Institute, Al-Mansour Middle Technical University, Baghdad, Iraq
چکیده
Mycobacterium Tuberculosis (TB) is one of the serious bacterial infections that cause diseases and may lead to death. In this study, 178 individuals were examined for TB infection at Baghdad TB center during the period from 15th January to 1st October 2021. Out of 178 participants, 73 were shown to be positive for TB infection, while 105 showed negative results. According to the results, there was no significant variation between infected males and females with TB in comparison to the control group (P>0.05). The results showed that the mean age of the patients for both males and females was in the range of 2–65 years. Additionally, there were significant differences in patients with TB compared to the control group in terms of the weight loss of 8.82 ± 6.75 Kg, red blood cell (RBC) count (3.43 ± 0.56) × 106/μl, white blood cell (WBC) count (3.12 ± 1.57) × 106/μl, platelet count (1.03 ± 0.56) × 106/μl, and hemoglobin level (6.66 ± 1.34) g/dl. A total of 30 TB patients and 50 normal individuals were genotyped to detect the IL-1β rs 114534 gene. The polymerase chain reaction (PCR) was used for exon amplification in region 5 of the ILB1 gene in the TB patients by using specific primers. The finding showed that there was an amplified product of 249bp located in chromosome 2q13-14. A total of 30 TB patients and 50 normal individuals were also genotyped to detect the IL-6 rs 1800795 gene. The PCR was used for amplification of the IL-6 gene in TB patients by using specific primers. The finding showed that there was an amplified product of 431 bp located in chromosome 7p15-p2. The expression of the ILB1 gene was investigated in TB patients and healthy controls by using qPT-PCR. Results showed that there was a high Ct value for patients and controls with a high Ct value of templates, preoperational to the total ribonucleic acid (RNA) concentration and gene expression. The expression of the IL-6 gene was investigated in TB patients and healthy controls by using qPT-PCR. Our findings revealed a high Ct value for patients and controls with a high Ct value of templates, preoperational to the total RNA concentration and gene expression.
کلیدواژه‌ها
IL-1β؛ IL-6 genes؛ Mycobacterium Tuberculosis
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مراجع
  1. Balaky STJ, Mawlood AH, Shabila NP. Survival analysis of patients with tuberculosis in Erbil, Iraqi Kurdistan region. BMC Infect Dis. 2019;19(1):1-8.
  2. Cao X-J, Li Y-P, Wang J-Y, Zhou J, Guo X-G. MPT64 assays for the rapid detection of Mycobacterium tuberculosis. BMC Infect Dis. 2021;21(1):1-15.
  3. Dunn JJ, Starke JR, Revell PA. Laboratory diagnosis of Mycobacterium tuberculosis infection and disease in children. J Clin Microbiol. 2016;54(6):1434-41.
  4. Gil-Santana L, Cruz LA, Arriaga MB, Miranda PF, Fukutani KF, Silveira-Mattos PS, et al. Tuberculosis-associated anemia is linked to a distinct inflammatory profile that persists after initiation of antitubercular therapy. Sci Rep. 2019;9(1):1-8.
  5. Gong W, Wu X. Differential diagnosis of latent tuberculosis infection and active tuberculosis: A key to a successful tuberculosis control strategy. Front Microbiol. 2021:3126.
  6. Hertz D, Dibbern J, Eggers L, von Borstel L, Schneider BE. Increased male susceptibility to Mycobacterium tuberculosis infection is associated with smaller B cell follicles in the lungs. Sci Rep. 2020;10(1):1-9.
  7. Khan FY. Review of literature on disseminated tuberculosis with emphasis on the focused diagnostic workup. J Fam Community Med. 2019;26(2):83.
  8. Kumar NP, Moideen K, Banurekha VV, Nair D, Babu S. Plasma proinflammatory cytokines are markers of disease severity and bacterial burden in pulmonary tuberculosis. InOpen forum infectious diseases 2019 Jul (Vol. 6, No. 7, p. ofz257). US: Oxford University Press.
  1. Warmelink I, Nick H, van der Werf TS, van Altena R. Weight loss during tuberculosis treatment is an important risk factor for drug-induced hepatotoxicity. Br J Nutr. 2011;105(3):400-8.
  2. Sousa J, Cá B, Maceiras AR, Simões-Costa L, Fonseca KL, Fernandes AI, et al. Mycobacterium tuberculosis associated with severe tuberculosis evades cytosolic surveillance systems and modulates IL-1β production. Nat Commun. 2020;11(1):1-14.
  3. Zhou F, Xu X, Wu S, Cui X, Fan L, Pan W. Protein array identification of protein markers for serodiagnosis of Mycobacterium tuberculosis infection. Sci Rep. 2015;5(1):1-10.
12.          Zhou F, Xu X, Cui X, Pan W. Development and Evaluation of a Fusion Polyprotein Based on HspX and Other Antigen Sequences for the Serodiagnosis of Tuberculosis. Front Microbiol. 2021:4128.

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