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تنوع سوماکلونال در گیاهک‌های باززایی شده از اندام‌های ساقه، ریشه و لپه لیلکی ایرانی (Gleditschia capsica Desf.) | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| مقاله 9، دوره 29، شماره 2 - شماره پیاپی 58، اسفند 1400، صفحه 268-281 اصل مقاله (875.47 K) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2022.355319.1388 | ||
| نویسندگان | ||
| مجتبی ایمانی راستابی1؛ سید محمد حسینی نصر* 2؛ غلامعلی رنجبر3؛ مصطفی خوشحال سرمست4 | ||
| 1دانش‌آموخته دکتری، گروه علوم و مهندسی جنگل، دانشکده منابع طبیعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، مازندران | ||
| 2نویسنده مسئول مکاتبات، دانشیار، گروه علوم و مهندسی جنگل، دانشکده منابع طبیعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، مازندران | ||
| 3دانشیار، گروه اصلاح نباتات، دانشکده علوم زراعی، دانشگاه علوم کشاورزی و منابع طبیعی ساری، مازندران | ||
| 4استادیار، گروه مهندسی علوم باغبانی و فضای سبز، دانشکده تولیدات گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، گلستان | ||
| چکیده | ||
| حفاظت از ژرمپلاسم گونه های درختی بومی جنگلهای هیرکانی به ویژه لیلکی ایرانی (Gleditschia capsica Desf.)، در اولویت پژوهش قرار دارد. مطالعات مولکولی درختان جنگلی در شرایط درون‌شیشه‌ای به‌ویژه در ایران بسیار اندک است. در این پژوهش، تنوع سوماکلونال در گیاهان باززایی شده از ریزنمونه های مختلف لیلکی ایرانی در شرایط درون‌شیشه‌ای با استفاده از نشانگر مولکولی تکرار توالی ساده (ISSR) بررسی شد. برگ پایه مادری (طبیعی) و ریزنمونه های ساقه، ریشه و لپه به‌عنوان ژنوتیپ‌های مورد مطالعه انتخاب و در محیط کشت MS حاوی 5/0 میلیگرم در لیتر TDZ و 5/0 میلیگرم در لیتر 2,4-D کشت شدند. پس از شش واکشت متوالی، تنوع سوماکلونال گیاهان باززایی شده از این ریزنمونه ها بررسی گردید. مشخصه های درصد چندشکلی (PIC)، تنوع نی و شانون برای بررسی تنوع بین ژنوتیپ ها استفاده شد و بعد با استفاده از الگوریتم UPMGA و تجزیه به مؤلفه های اصلی (PCA) خوشهبندی و تفکیک ژنوتیپ ها انجام شد. با استفاده از 10 آغازگر، درصد چندشکلی بین گیاهان باززایی‌شده به‌میزان 9/87 درصد به‌دست آمد. تجزیه‌وتحلیل واریانس مولکولی (AMOVA)، 16 درصد تنوع بین ژنوتیپ‌ها با پایه مادری را نشان داد. نتایج آنالیز خوشه‌ای بهروش UPGMA، ژنوتیپ‌ها را در سه دسته مجزا گروه‌بندی کرد. بر این اساس ژنوتیپ‌های پایه مادری و ژنوتیپ‌های ساقه در یک گروه قرار گرفتند. پایه مادری بیشترین و کمترین ضریب تشابه را به‌ترتیب با ژنوتیپ‌های ساقه و ریشه داشت. بنابراین پیشنهاد می‌شود برای مطالعات کشت بافت لیلکی ایرانی از ریزنمونه‌های ساقه که تشابه ژنتیکی بالایی با ژنوم مادری دارد استفاده شود. به‌طورکلی نتایج نشان داد که تنوع سوماکلونال در بین گیاهان باززایی‌شده با افزایش تعداد دفعات واکشت افزایش خواهد یافت | ||
| کلیدواژه‌ها | ||
| تنظیم کننده‌های رشد؛ تنوع ژنتیکی؛ درون شیشه‌ای؛ لیلکی ایرانی؛ نشانگر مولکولی | ||
| عنوان مقاله [English] | ||
| Somaclonal diversity in regenerated plants from stem, root and cotyledons of Caspian locust (Gleditschia capsica Desf.) | ||
| نویسندگان [English] | ||
| M. Imani rastabi1؛ S. M. Hosseini nasr2؛ G. A. Ranjbar3؛ M. Khoshhal Sarmast4 | ||
| 1Ph.D. graduated, Department of Forest Science and Engineering, Agricultural Science Faculty, Sari University of Agricultural Sciences and Natural Resources, Mazandaran, I.R. Iran | ||
| 2Corresponding author, Assoc. Prof., Department of Forest Science and Engineering, Agricultural Science Faculty, Sari University of Agricultural Sciences and Natural Resources, Mazandaran, I.R. Iran | ||
| 3Assoc. Prof., Department of Plant Breeding, Agricultural Science Faculty, Sari University of Agricultural Sciences and Natural Resources, Mazandaran, I.R. Iran. | ||
| 4Assist Prof, Department of Horticultural and Green Space Engineering, Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Golestan, I.R. Iran. | ||
| چکیده [English] | ||
| Protecting the germplasm of native tree species of the Caspian hyrcanian forests, especially the Caspian locust (Gleditschia capsica Desf.) is a priority of the research. In this study, somaclonal diversity in regenerated plants from different explants of Caspian locust was investigated using the Inter Simple Sequence Repeat (ISSR) marker under in vitro conditions. Mother leaf (natural) and stem, root and cotyledon explants from the regenerated plants as studied genotypes were selected and they were cultured in MS culture medium containing 0.5 mgL-1 TDZ and 0.5 mgl1 2,4-D. Somaclonal diversity was examined after six subculture inoculations. Polymorphism (PIC), Nei and Shannon diversity traits were used to investigate the diversity between genotypes and then clustering of genotypes was performed using UPMGA algorithm and Principal Component Analysis (PCA). The polymorphism percentage was 87.9% using 10 primers. Molecular Analysis of Variance (AMOVA) showed 16% diversity between genotypes and maternal origin. The results of cluster analysis grouped the genotypes into three distinct categories, which was also confirmed by principal component analysis (PCA). Accordingly, the mother basal and stem genotypes were grouped in a group. The mother basal had the highest and lowest similarity coefficients with stem and root genotypes, respectively. Therefore, it was suggested to use stem explants that have high genetic similarity with the mother genome for studies of Caspian locust tissue culture. In general, the results showed that somaclonal diversity among regenerated plants will increase with increasing number of subcultured. | ||
| کلیدواژه‌ها [English] | ||
| Plant growth regulators (PGRs), genetic diversity, in vitro, Gleditschia capsica Desf, molecular marker | ||
| مراجع | ||
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