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Abed, S. M, Jabar Jasim, H, Mijbas Mohammed Alomari, M, Hamoodi Hussein, G. (1401). Comparison between Antimicrobial and Antibiofilm Activity of Exopolysaccharides (EPS) Extracted from Lactobacillus reuteri and Streptococcus mitis against Oral Bacteria. سامانه مدیریت نشریات علمی, 77(6), 2215-2221. doi: 10.22092/ari.2022.358341.2203
S. M Abed; H Jabar Jasim; M Mijbas Mohammed Alomari; G Hamoodi Hussein. "Comparison between Antimicrobial and Antibiofilm Activity of Exopolysaccharides (EPS) Extracted from Lactobacillus reuteri and Streptococcus mitis against Oral Bacteria". سامانه مدیریت نشریات علمی, 77, 6, 1401, 2215-2221. doi: 10.22092/ari.2022.358341.2203
Abed, S. M, Jabar Jasim, H, Mijbas Mohammed Alomari, M, Hamoodi Hussein, G. (1401). 'Comparison between Antimicrobial and Antibiofilm Activity of Exopolysaccharides (EPS) Extracted from Lactobacillus reuteri and Streptococcus mitis against Oral Bacteria', سامانه مدیریت نشریات علمی, 77(6), pp. 2215-2221. doi: 10.22092/ari.2022.358341.2203
Abed, S. M, Jabar Jasim, H, Mijbas Mohammed Alomari, M, Hamoodi Hussein, G. Comparison between Antimicrobial and Antibiofilm Activity of Exopolysaccharides (EPS) Extracted from Lactobacillus reuteri and Streptococcus mitis against Oral Bacteria. سامانه مدیریت نشریات علمی, 1401; 77(6): 2215-2221. doi: 10.22092/ari.2022.358341.2203

Comparison between Antimicrobial and Antibiofilm Activity of Exopolysaccharides (EPS) Extracted from Lactobacillus reuteri and Streptococcus mitis against Oral Bacteria

Archives of Razi Institute
مقاله 25، دوره 77، شماره 6، بهمن و اسفند 2022، صفحه 2215-2221    اصل مقاله (496.53 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.358341.2203
نویسندگان
S. M Abed1؛ H Jabar Jasim* 2؛ M Mijbas Mohammed Alomari2؛ G Hamoodi Hussein1
1Department of Medical Laboratories, College of Medical and Health Techniques, Sawa University, Samawah, Iraq
2College of Veterinary Medicine, Al-Muthanna University, Samawah, Iraq
چکیده
Due to the increased resistance to antibiotics and chemical biocides, the use of bacterial exopolysaccharides has been considered. The objective of the current study was to investigate the strength of the antibacterial and antibiofilm activity of EPS extracted from Lactobacillus reuteri and Streptococcus mitis because previous studies showed there were structural differences between EPS, during this study, EPS extracted from Lactobacillus reuteri and Streptococcus miti by ethanol precipitation method and estimated antibacterial and antibiofilm activity against several Oral Bacteria (Staphylococcus aureus, Staphylococcus hominis, Acinetobacter baumannii, Raoultella ornithinolytica, Streptococcus thoraltensis in different concentration as (100,150,200,250,300 mg/ml ), the results showed carbohydrates rate in extracted  EPS from L. reuteri  and S. mitis were recorded was 85, 80 % respectively.The concentration 100 and 150 mg/ml for EPS from L. reuteri  and S. mitis, there was no inhibitory effect, except in S. aureus (1.1±0.10)  and S. hominis (1.0±0.10) at 100 mg/ml, 3.1±2.01, 2.1±0.54 mg/ml respectively at 150 mg/ml concentration but no significant differences (P≤0.05). However, the antibacterial effect of that EPSs started at the concentration of 200 and upwards, where different results were recorded between the concentrations of both EPSs against all bacteria isolated (P≤0.05), On the other hand, the effect of EPS from L. reuteri  and S. mitis was clear against the formation of biofilm compared with the control, worth mentioning that EPS from L. reuteri was more effective compared with EPS from S. mitis in all isolates (P≤0.05) except for S. thoraltensis where it was noted that the EPS from S. mitis is more effective than EPS from L. reuteri. Through the results obtained in this study, it was noted that the difference in the structural nature of EPS has an important role in its effectiveness as an antibacterial and anti-biofilm formation and, as it was found that the EPS from L. reuteri showed more effectiveness than EPS from S. mitis and thus the mechanism of preventing and inhibiting bacteria depending of the structural nature of EPS.
کلیدواژه‌ها
EPS؛ Lactobacillus reuteri؛ Streptococcus Mitis؛ Antibacterial؛ Antibiofilm
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مراجع
  1. Chen Z, Shi J, Yang X, Liu Y, Nan B, Wang Z. Isolation of exopolysaccharide-producing bacteria and yeasts from Tibetan kefir and characterisation of the exopolysaccharides. Int J Dairy Technol. 2016;69:n/a-n/a.
  2. Silva LA, Lopes Neto JHP, Cardarelli HR. Exopolysaccharides produced by Lactobacillus plantarum: technological properties, biological activity, and potential application in the food industry. Ann Microbiol. 2019;69(4):321-8.
  3. Kanmani P, Lim ST. Synthesis and structural characterization of silver nanoparticles using bacterial exopolysaccharide and its antimicrobial activity against food and multidrug resistant pathogens. Process Biochem. 2013;48(7):1099-106.
  4. Djokovic V, Krsmanovic R, Bozanic DK, McPherson M, Van Tendeloo G, Nair PS, et al. Adsorption of sulfur onto a surface of silver nanoparticles stabilized with sago starch biopolymer. Colloids Surf B Biointerfaces. 2009;73(1):30-5.
  5. Sanalibaba P, Cakmak GA. Exopolysaccharides production by lactic acid bacteria. Appl Microbiol Open Access. 2016;2.
  6. Surayot U, Wang J, Seesuriyachan P, Kuntiya A, Tabarsa M, Lee Y, et al. Exopolysaccharides from lactic acid bacteria: structural analysis, molecular weight effect on immunomodulation. Int J Biol Macromol. 2014;68:233-40.
  7. Wang K, Niu M, Song D, Song X, Zhao J, Wu Y, et al. Preparation, partial characterization and biological activity of exopolysaccharides produced from Lactobacillus fermentum S1. J Biosci Bioeng. 2020;129(2):206-14.
  1. Panghal A, Janghu S, Virkar K, Gat Y, Kumar V, Chhikara N. Potential non-dairy probiotic products – A healthy approach. Food Biosci. 2018;21:80-9.
  2. Ciszek-Lenda M. Biological functions of exopolysaccharides from probiotic bacteria. Cent Eur J Immunol. 2011;36:51-5.
  3. London LE, Kumar AH, Wall R, Casey PG, O'Sullivan O, Shanahan F, et al. Exopolysaccharide-producing probiotic Lactobacilli reduce serum cholesterol and modify enteric microbiota in ApoE-deficient mice. J Nutr. 2014;144(12):1956-62.
  4. Torino MI, Font de Valdez G, Mozzi F. Biopolymers from lactic acid bacteria. Novel applications in foods and beverages. Front Microbiol. 2015;6:834.
  5. Lynch KM, Coffey A, Arendt EK. Exopolysaccharide producing lactic acid bacteria: Their techno-functional role and potential application in gluten-free bread products. Food Res Int. 2018;110:52-61.
  6. Daba GM, Elnahas MO, Elkhateeb WA. Contributions of exopolysaccharides from lactic acid bacteria as biotechnological tools in food, pharmaceutical, and medical applications. Int J Biol Macromol. 2021;173:79-89.
  7. Bajpai VK, Majumder R, Rather I, Kim K. Extraction, isolation and purification of exopolysaccharide from lactic acid bacteria using ethanol precipitation method. Bangladesh J Pharmacol. 2016;11:573.
  8. Wiegand I, Hilpert K, Hancock RE. Agar and broth dilution methods to determine the minimal inhibitory concentration (MIC) of antimicrobial substances. Nat Protoc. 2008;3(2):163-75.
  9. Tetz GV, Artemenko NK, Tetz VV. Effect of DNase and antibiotics on biofilm characteristics. Antimicrob Agents Chemother. 2009;53(3):1204-9.
  10. Hanan MM, Saida MA. Purification And Characterization Of Exopolysaccharides (Eps) Extracted From Saccharomyces Cerevisiae. Egypt J Exp Biol. 2013;9(2): 249-58.
  11. Dilna SV, Surya H, Aswathy RG, Varsha KK, Sakthikumar DN, Pandey A, et al. Characterization of an exopolysaccharide with potential health-benefit properties from a probiotic Lactobacillus plantarum RJF4. LWT-Food Sci Technol. 2015;64(2):1179-86.
  12. Welman AD, Maddox IS. Fermentation performance of an exopolysaccharide-producing strain of Lactobacillus delbrueckii subsp. bulgaricus. J Ind Microbiol Biotechnol. 2003;30(11):661-8.
  1. Abdalla AK, Ayyash MM, Olaimat AN, Osaili TM, Al-Nabulsi AA, Shah NP, et al. Exopolysaccharides as Antimicrobial Agents: Mechanism and Spectrum of Activity. Front Microbiol. 2021;12:664395.
  2. Nguyen PT, Nguyen TT, Bui DC, Hong PT, Hoang QK, Nguyen HT. Exopolysaccharide production by lactic acid bacteria: the manipulation of environmental stresses for industrial applications. AIMS Microbiol. 2020;6(4):451-69.
  3. Zhang J, Cao Y, Wang J, Guo X, Zheng Y, Zhao W, et al. Physicochemical characteristics and bioactivities of the exopolysaccharide and its sulphated polymer from Streptococcus thermophilus GST-6. Carbohydr Polym. 2016;146:368-75.
  4. Riaz Rajoka MS, Wu Y, Mehwish HM, Bansal M, Zhao L. Lactobacillus exopolysaccharides: New perspectives on engineering strategies, physiochemical functions, and immunomodulatory effects on host health. Trends Food Sci Technol. 2020;103:36-48.
  5. Wu MH, Pan TM, Wu YJ, Chang SJ, Chang MS, Hu CY. Exopolysaccharide activities from probiotic bifidobacterium: Immunomodulatory effects (on J774A.1 macrophages) and antimicrobial properties. Int J Food Microbiol. 2010;144(1):104-10.
  6. Rani RP, Anandharaj M, David Ravindran A. Characterization of a novel exopolysaccharide produced by Lactobacillus gasseri FR4 and demonstration of its in vitro biological properties. Int J Biol Macromol. 2018;109:772-83.
  7. Ayyash M, Abu-Jdayil B, Itsaranuwat P, Galiwango E, Tamiello-Rosa C, Abdullah H, et al. Characterization, bioactivities, and rheological properties of exopolysaccharide produced by novel probiotic Lactobacillus plantarum C70 isolated from camel milk. Int J Biol Macromol. 2020;144:938-46.
  8. Sivasankar P, Seedevi P, Poongodi S, Sivakumar M, Murugan T, Sivakumar L, et al. Characterization, antimicrobial and antioxidant property of exopolysaccharide mediated silver nanoparticles synthesized by Streptomyces violaceus MM72. Carbohydr Polym. 2018;181:752-9.
  9. Medrano M, Hamet MF, Abraham AG, Perez PF. Kefiran protects Caco-2 cells from cytopathic effects induced by Bacillus cereus infection. Antonie Van Leeuwenhoek. 2009;96(4):505-13.
  1. Salachna P, Mizielinska M, Sobol M. Exopolysaccharide Gellan Gum and Derived Oligo-Gellan Enhance Growth and Antimicrobial Activity in Eucomis Plants. Polymers (Basel). 2018;10(3).
  2. Zhou Y, Cui Y, Qu X. Exopolysaccharides of lactic acid bacteria: Structure, bioactivity and associations: A review. Carbohydr Polym. 2019;207:317-32.
  3. Juraskova D, Ribeiro SC, Silva CCG. Exopolysaccharides Produced by Lactic Acid Bacteria: From Biosynthesis to Health-Promoting Properties. Foods. 2022;11(2).
  4. Liu Z, Zhang Z, Qiu L, Zhang F, Xu X, Wei H, et al. Characterization and bioactivities of the exopolysaccharide from a probiotic strain of Lactobacillus plantarum WLPL04. J Dairy Sci. 2017;100(9):6895-905.
  5. Xu X, Peng Q, Zhang Y, Tian D, Zhang P, Huang Y, et al. Antibacterial potential of a novel Lactobacillus casei strain isolated from Chinese northeast sauerkraut and the antibiofilm activity of its exopolysaccharides. Food Funct. 2020;11(5):4697-706.
  6. Pan D, Mei X. Antioxidant activity of an exopolysaccharide purified from Lactococcus lactis subsp. lactis 12. Carbohydr Polym. 2010;80(3):908-14.
  7. Mahdhi A, Leban N, Chakroun I, Chaouch MA, Hafsa J, Fdhila K, et al. Extracellular polysaccharide derived from potential probiotic strain with antioxidant and antibacterial activities as a prebiotic agent to control pathogenic bacterial biofilm formation. Microb Pathog. 2017;109:214-20.
  8. Kim Y, Oh S, Kim SH. Released exopolysaccharide (r-EPS) produced from probiotic bacteria reduce biofilm formation of enterohemorrhagic Escherichia coli O157:H7. Biochem Biophys Res Commun. 2009;379(2):324-9.
  9. James KM, MacDonald KW, Chanyi RM, Cadieux PA, Burton JP. Inhibition of Candida albicans biofilm formation and modulation of gene expression by probiotic cells and supernatant. J Med Microbiol. 2016;65(4):328-36.
  10. Jiang G, Gan L, Li X, He J, Zhang S, Chen J, et al. Characterization of Structural and Physicochemical Properties of an Exopolysaccharide Produced by Enterococcus sp. F2 From Fermented Soya Beans. Front Microbiol. 2021;12:744007.
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