اخبار و اعلانات

 آمار

تعداد نشریات286
تعداد نشریات سازمان84
تعداد شماره‌ها3,019
تعداد مقالات33,734
تعداد مشاهده مقاله44,973,849
تعداد دریافت فایل اصل مقاله46,449,550
Mohammed Ibrahim, O, Bara Allawe, A, Ali Kadhim, H. (1401). Isolation and Molecular Detection of Feline Infectious Peritonitis Virus. سامانه مدیریت نشریات علمی, 77(5), 1709-1714. doi: 10.22092/ari.2022.357997.2135
O Mohammed Ibrahim; A Bara Allawe; H Ali Kadhim. "Isolation and Molecular Detection of Feline Infectious Peritonitis Virus". سامانه مدیریت نشریات علمی, 77, 5, 1401, 1709-1714. doi: 10.22092/ari.2022.357997.2135
Mohammed Ibrahim, O, Bara Allawe, A, Ali Kadhim, H. (1401). 'Isolation and Molecular Detection of Feline Infectious Peritonitis Virus', سامانه مدیریت نشریات علمی, 77(5), pp. 1709-1714. doi: 10.22092/ari.2022.357997.2135
Mohammed Ibrahim, O, Bara Allawe, A, Ali Kadhim, H. Isolation and Molecular Detection of Feline Infectious Peritonitis Virus. سامانه مدیریت نشریات علمی, 1401; 77(5): 1709-1714. doi: 10.22092/ari.2022.357997.2135

Isolation and Molecular Detection of Feline Infectious Peritonitis Virus

Archives of Razi Institute
مقاله 24، دوره 77، شماره 5، آذر و دی 2022، صفحه 1709-1714    اصل مقاله (856.75 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.357997.2135
نویسندگان
O Mohammed Ibrahim* 1؛ A Bara Allawe1؛ H Ali Kadhim2
1Department of Microbiology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq
2Department of Surgery and Obstetrics, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq
چکیده
Feline coronavirus (FCoV) is an enveloped single-stranded RNA virus, affecting wild and domestic cats. Feline infectious peritonitis viruses (FIPV) variants of FCoV cause fatal peritonitis affecting approximately 5% of FCoV infected animals. The present study aimed to detect and isolate the feline infectious peritonitis virus for the first time in Iraq. In this study, 50 samples (fecal swab and peritoneal fluid) were collected from suspected pet cats from different areas of Baghdad, Iraq. The very suitable age was under two years old.  Real-time reverse transcription-polymerase chain reaction (real-time RT-PCR) was used to detect Feline infectious peritonitis in infected collected samples by the amplification of spike protein (S). The result of real-time RT-PCR revealed that out of 50 samples from suspected cats, 10 samples were positive for FIPV. Moreover, 10 positive samples by real-time RT-PCR were used for the isolation of the virus in chicken embryo fibroblast cell culture. Subsequently, the isolated virus was detected by real-time RT-PCR and then by conventional RT-PCR, followed by electrophoresis.
کلیدواژه‌ها
Cell culture؛ Feline coronavirus؛ Feline infectious peritonitis virus؛ Real-time RT-PCR
مراجع
  1. Sykes JE. Feline coronavirus infection. Canine and feline infectious diseases. 2014:195.
  2. AL-Mutar HA. The evolution of Genetic Molecular Map and phylogenetic tree of Coronavirus (COVID-19). Iraqi J Vet Sci. 2020;44(2):56-70.
  3. Payne S. Family coronaviridae. Viruses. 2017:149.
  4. Dye C, Siddell SG. Genomic RNA sequence of Feline coronavirus strain FIPV WSU-79/1146. J Gen Virol. 2005;86(Pt 8):2249.
  5. Delaplace M, Huet H, Gambino A, Le Poder S. Feline Coronavirus Antivirals: A Review. Pathogens. 2021;10(9):1150.
  6. Hartmann K. Feline infectious peritonitis. Vet Clin North Am Small Anim Pract. 2005;35(1):39-79.
  7. Sharif S, Arshad SS, Hair-Bejo M, Omar AR, Zeenathul NA, Alazawy A. Diagnostic methods for feline coronavirus: a review. Vet Med Int. 2010;2010.
  8. Pedersen NC. A review of feline infectious peritonitis virus infection: 1963–2008. J Feline Med Surg. 2009;11(4):225-58.
  9. Diaz JV, Poma R. Diagnosis and clinical signs of feline infectious peritonitis in the central nervous system. Can Vet J. 2009;50(10):1091.
  10. Herrewegh A, De Groot R, Cepica A, Egberink HF, Horzinek MC, Rottier P. Detection of feline coronavirus RNA in feces, tissues, and body fluids of naturally infected cats by reverse transcriptase PCR. J Clin Microbiol. 1995;33(3):684-9.
  11. Addie D, Schaap I, Nicolson L, Jarrett O. Persistence and transmission of natural type I feline coronavirus infection. J Gen Virol. 2003;84(10):2735-44.
  1. Horsburgh BC, Brierley I, Brown TDK. Analysis of a 9.6 kb sequence from the 3′ end of canine coronavirus genomic RNA. J Gen Virol. 1992;73(11):2849-62.
  2. Jacobse-Geels H, Horzinek M. Expression of feline infectious peritonitis coronavirus antigens on the surface of feline macrophage-like cells. J Gen Virol. 1983;64(9):1859-66.
  3. Pedersen N. An overview of feline enteric coronavirus and infectious peritonitis virus infections. Feline Pract. 1995;23:7-20.
  4. Pedersen N, Evermann J, McKeirnan A, Ott R. Pathogenicity studies of feline coronavirus isolates 79-1146 and 79-1683. Am J Vet Res. 1984;45(12):2580-5.
  5. Evermann JF, Heeney JL, McKeirnan AJ, O'Brien SJ. Comparative features of a coronavirus isolated from a cheetah with feline infectious peritonitis. Virus Res. 1989;13(1):15-27.
  6. Ksiazek TG, Erdman D, Goldsmith CS, Zaki SR, Peret T, Emery S, et al. A novel coronavirus associated with severe acute respiratory syndrome. N Engl J Med. 2003;348(20):1953-66.
  1. Woods RD, Wesley RD. Cultivation techniques for animal coronaviruses: emphasis on feline infectious peritonitis virus, canine coronavirus, transmissible gastroenteritis virus, and porcine hemagglutinating encephalomyelitis virus. J Tissue Cult Methods. 1988;11(2):95-100.
  2. Bell E, Toribio J, White J, Malik R, Norris J. Seroprevalence study of feline coronavirus in owned and feral cats in Sydney, Australia. Aust Vet J. 2006;84(3):74-81.
  3. Lin C-N, Su B-L, Wang C-H, Hsieh M-W, Chueh T-J, Chueh L-L. Genetic diversity and correlation with feline infectious peritonitis of feline coronavirus type I and II: a 5-year study in Taiwan. Vet Microbiol. 2009;136(3-4):233-9.
  4. Shiba N, Maeda K, Kato H, Mochizuki M, Iwata H. Differentiation of feline coronavirus type I and II infections by virus neutralization test. Vet Microbiol. 2007;124(3-4):348-52.
آمار
تعداد مشاهده مقاله: 1,984
تعداد دریافت فایل اصل مقاله: 1,781


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب