An overview of genome editing methods | ||
| علوم و فنون دامپروری | ||
| Volume 10, Issue 41, March 2022, Pages 13-28 PDF (1.52 M) | ||
| DOI: 10.22092/aasrj.2021.354669.1236 | ||
| Authors | ||
| Hamed Asadollahi* 1; Hossein Emrani2 | ||
| 1Department of Animal Science, Faculty of Agriculture, Isfahan University of Technology, Isfahan, Iran | ||
| 2Animal Science Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), 31585 Karaj, Iran | ||
| Abstract | ||
| With the advent of gene editing methods, including ZFN, TALEN and CRISPR-Cas9 technologies, the genomes of different species can be easily edited. Of these methods, are used in inactivation of specific genes in livestock to improve animal production traits and increase productivity, Improving various human diseases, reduce the transmission of common human and animal diseases, Inactivation of genes involved in various infections, resistance to pathogens, and finally modification and treatment of complex genetic disorders. To achieve the capabilities of these editing mechanisms, some limitations need to be improved, such as specific targeting, targeted nuclease enzyme transfer, donor DNA sequencing, and DNA repair mechanisms. In fact, transmission quality, identification of specific targets, better understanding of DNA editing mechanisms, and identification of ways to enhance the mechanism of matched recombination repair are the main focus of genomic editing systems. These advances have provided countless opportunities to help us understand the complex functions of genes and disease mechanisms, identify different pathogenic mutations, and modify agricultural and livestock products. | ||
| Keywords | ||
| Genome editing; ZFN; TALEN; CRISPR-Cas9 | ||
| References | ||
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