اخبار و اعلانات

 آمار

تعداد نشریات284
تعداد نشریات سازمان82
تعداد شماره‌ها3,081
تعداد مقالات34,377
تعداد مشاهده مقاله48,004,770
تعداد دریافت فایل اصل مقاله78,922,500
احمدی, لیلی, Kazem, کاظم, بهارلوئی, ژیلا, داودی, محمد حسین. (1401). اثر متابولیت‌های حاصل از تجزیه میکروبی پر مرغ بر رشد کاهو. سامانه مدیریت نشریات علمی, 10(2), 229-241. doi: 10.22092/sbj.2022.355784.223
لیلی احمدی; کاظم Kazem; ژیلا بهارلوئی; محمد حسین داودی. "اثر متابولیت‌های حاصل از تجزیه میکروبی پر مرغ بر رشد کاهو". سامانه مدیریت نشریات علمی, 10, 2, 1401, 229-241. doi: 10.22092/sbj.2022.355784.223
احمدی, لیلی, Kazem, کاظم, بهارلوئی, ژیلا, داودی, محمد حسین. (1401). 'اثر متابولیت‌های حاصل از تجزیه میکروبی پر مرغ بر رشد کاهو', سامانه مدیریت نشریات علمی, 10(2), pp. 229-241. doi: 10.22092/sbj.2022.355784.223
احمدی, لیلی, Kazem, کاظم, بهارلوئی, ژیلا, داودی, محمد حسین. اثر متابولیت‌های حاصل از تجزیه میکروبی پر مرغ بر رشد کاهو. سامانه مدیریت نشریات علمی, 1401; 10(2): 229-241. doi: 10.22092/sbj.2022.355784.223

اثر متابولیت‌های حاصل از تجزیه میکروبی پر مرغ بر رشد کاهو

زیست شناسی خاک
دوره 10، شماره 2، مهر 1401، صفحه 229-241    اصل مقاله (519.72 K)
نوع مقاله: مقاله پژوهشی
شناسه دیجیتال (DOI): 10.22092/sbj.2022.355784.223
نویسندگان
لیلی احمدی1؛ کاظم Kazem* 2؛ ژیلا بهارلوئی3؛ محمد حسین داودی4
1دانشجوی گروه خاکشناسی، دانشگاه آزاد اسلامی، واحد اصفهان ( خوراسگان
2استاد مؤسسه تحقیقات خاک و آب، سازمان تحقیقات، آموزش و ترویج کشاورزی
3استادیار گروه خاکشناسی، دانشگاه آزاد اسلامی، واحد اصفهان ( خوراسگان)
4استادیار مؤسسه تحقیقات خاک و آب، سازمان تحقیقات، آموزش و ترویج کشاورزی
چکیده
در این مطالعه برای تولید کود با مواد طبیعی، از باکتری‌های تولیدکننده آنزیم کراتیناز به‌منظور تجزیه پر مرغ که متشکل از پروتئین کراتین است استفاده گردید. تعداد 29 نمونه خاک از عمق صفر تا سی سانتی متری مزارع کشاورزی جمع‌آوری شد و با پر مرغ مخلوط گردید. پس از سه هفته از خاک‌های مخلوط با پر ۳۱ جدایه که قادر به رشد بر روی محیط کشت اختصاصی پودر پر جامد (Feather Meal Agar, FMA) بودند جداسازی شدند. ایزوله­ها به محیط مایع حاوی پر انتقال داده شدند و توان تجزیه پر توسط آنها بررسی گردید. تأثیر محلول حاصل از تجزیه پر بر رشد کاهو در قالب طرح کاملاً تصادفی در گلخانه بررسی شد. تیمارهای آزمایش شامل محلول‌پاشی با سه محلول حاصل از تجزیه پر مرغ (gh1، b1، c11) و محلول‌پاشی با آب مقطر (شاهد) بودند. نتایج نشان داد تعداد هشت جدایه با تولید آنزیم کراتیناز قادر به تجزیه کامل پر در طول هفت روز بودند. بالاترین فعالیت آنزیم کراتیناز با تجزیه کامل پر معادل U/ml ۵۶/۸ مربوط به جدایه Bacillus methylotrophicus gh1 بود. همچنین حداکثر غلظت اسیدآمینه آزاد، در محیط کشت جدایه Bacillus siamensis c11 معادل μg/ml 1065 مشاهده شد. سه محلول حاصل از تجزیه پر تأثیر معنی‌داری در سطح یک درصد بر روی وزن ‌تر کاهو، وزن خشک کاهو و وزن‌ تر ریشه داشتند. در تیمارهای Bacillus methylotrophicus gh1، b1 Bacillus velezensis و Bacillus siamensis c11 وزن‌ تر کاهو به ترتیب 8/28%، 1/26% و 1/14% و وزن خشک کاهو به­ترتیب 7/25%، 9/19% و 2/۱۵% نسبت به تیمار شاهد افزایش نشان دادند. یافته‌ها نشان داد با استفاده از باکتری‌های تولیدکننده آنزیم کراتیناز می توان تجزیه پر را افزایش داده و از محصول حاصل به‌عنوان محرک رشد کاهو استفاده نمود.
کلیدواژه‌ها
اسیدآمینه آزاد؛ خاک؛ کراتین؛ محرک رشد
عنوان مقاله [English]
Effect of metabolites obtained from microbial degradation of chicken feathers on lettuce growth
نویسندگان [English]
Leili Ahmadi1؛ kazam Khavazi2؛ Jila Baharlouei3؛ Mohammad Hossein Davoodi4
1PhD student, Department of Soil Science, Islamic Azad University, Isfahan (Khorasgan) Branch
2Professor, Soil and Water Research Institute, Agricultural Research Education and Extension Organization
3Assistant Professor, Department of Soil Science, Islamic Azad University, Isfahan (Khorasgan) Branch
4Assistant Professor, Soil and Water Research Institute, Agricultural Research Education and Extension Organization
چکیده [English]
In this study, keratinase-producing bacteria were used to produce natural fertilizer from chicken feathers. Twenty-nine soil samples were collected from 0-30 cm depth of agricultural fields and mixed with chicken feathers. After three weeks, 31 isolates were isolated from the soils mixed with feathers, which were able to grow on the special culture medium of Feather Meal Agar (FMA). The isolates were transferred to a liquid medium containing feathers and their ability to degrade the feathers was investigated. A completely randomized design was used to examine the effect of the solution created by the degradation of chicken feathers on lettuce growth. The experimental treatments included the foliar application of three solutions obtained from the degradation of chicken feathers (gh1, b1, c11), and control (distilled water). The results showed eight isolates were able to completely decompose the feathers in seven days by producing keratinase enzyme. The highest activity of the keratinase enzyme (with the ability to fully degrade the feather: 8.56 U / ml) was related to that of Bacillus methylotrophic strain gh1. Also, the maximum concentration of free amino acid (1065 μg / ml) was observed in the growth medium of Bacillus siamensis strain c11. The three solutions obtained from feather degradation had a significant effect (p < 0.01) on lettuce fresh weight, lettuce dry weight, and fresh root weight. The results showed that the lettuce fresh weight increased by 28.8%, 26.1%, and 14.1%, using Bacillus methylotrophicus (gh1), Bacillus velezensis (b1), and Bacillus siamensis (c11) respectively, compared to the control. In addition, the lettuce dry weight increased by 25.7%, 19.9%, and 15.2%, with the aid of gh1, b1, and c11 treatments, respectively, compared to the control. The results showed that using keratinase-producing bacteria, feather degradation could be increased, and the obtained product can be used as a growth stimulant for lettuce.
کلیدواژه‌ها [English]
Free amino acid, Growth stimulant, Keratin, Soil
مراجع
  1. امامی، ع. 1375. روش‌های تجزیه گیاه. موسسه تحقیقات آب ‌و خاک. نشریه شماره 8-90.
  2. Adelere, I.A. and Lateef, A. 2016. Keratinases: emerging trends in production and applications as novel multifunctional biocatalysts. Kuwait Journal of Science 43.
  3. Amoozgar, A., Mohammadi, A. and Sabzalian, M.R. Impact of light-emitting diode irradiation on photosynthesis, phytochemical composition and mineral element content of lettuce cv. Grizzly. Photosynthetica 55:85-95.
  4. Anbu, P., Gopinath, S.C.B., Hilda, A., Lakshmipriya, T. and Annadurai, G. 2007. Optimization of extra cellular keratinase production by poultry farm isolate Scopulariopsis brevicaulis. Bioresource Technology 98:1298-1303.
  5. Ardyati, T. 2019, December. Screening of Keratinolytic Fungi for Biodegradation Agent of Keratin from Chicken Feather Waste. In IOP Conference Series: Earth and Environmental Science (Vol. 391, No. 1, p. 012027). IOP Publishing.
  6. Arkhipchenko, I.A., Salkinoja-Salonen, M.S., Karyakina, J.N. and Tsitko, I. 2005. Study of three fertilizers produced from farm waste. Applied Soil Ecology 30:126-132.
  7. Bach, E., Daroit, D.J., Corrêa, A.P.F. and Brandelli, A. 2011. Production and properties of keratinolytic proteases from three novel Gram-negative feather-degrading bacteria isolated from Brazilian soils. Biodegradation 22:1191-1201.
  8. Bhange, K., Chaturvedi, V. and Bhatt, R. 2016. Ameliorating effects of chicken feathers in plant growth promotion activity by a keratinolytic strain of Bacillus subtilis Bioresources and Bioprocessing 3:1-10.
  9. Bhari, R., Kaur, M. and Singh, R.S. 2021. Chicken feather waste hydrolysate as a superior biofertilizer in agroindustry. Current Microbiology 1-19.
  10. Bose, A., Pathan, S., Pathak, K. and Keharia, H. 2014. Keratinolytic protease production by Bacillus amyloliquefaciens 6B using feather meal as substrate and application of feather hydrolysate as organic nitrogen input for agricultural soil. Waste and Biomass Valorization 5:595-605.
  11. Brandelli, A., Daroit, D.J. and Riffel, A. 2010. Biochemical features of microbial keratinases and their production and applications. Applied Microbiology and Biotechnology 85:1735-1750.
  12. Brandelli, A., 2008. Bacterial keratinases: useful enzymes for bioprocessing agroindustrial wastes and beyond. Food and Bioprocess Technology 1:105-116.
  13. Cao, Z.J., Lu, D., Luo, L.S., Deng, Y.X., Bian, Y.G., Zhang, X.Q. and Zhou, M.H. 2012. Composition analysis and application of degradation products of whole feathers through a large scale of fermentation. Environmental Science and Pollution Research 19:2690-2696.
  14. Checa‐Moreno, R., Manzano, E., Mirón, G. and Capitán‐Vallvey, L.F. 2008. Revisitation of the phenylisothiocyanate‐derivatives procedure for amino acid determination by HPLC‐ Journal of Separation Science 31: 3817-3828.
  15. Deydier, E., Guilet, R., Sarda, S. and Sharrock, P. 2005. Physical and chemical characterisation of crude meat and bone meal combustion residue:“waste or raw material?”. Journal of Hazardous Materials 121:141-148.
  16. 2012. Lettuce and chicory production quantity. Available online at http://faosta3.fao.org/ home/index.html≠download .Accessed 2 October 2016.
  17. González-Castro, M.J., López-Hernández, J., Simal-Lozano, J. and Oruña-Concha, M.J. 1997. Determination of amino acids in green beans by derivatization with phenylisothiocianate and high-performance liquid chromatography with ultraviolet detection. Journal of Chromatographic Science 35:181-185.
  18. Gupta, R., Sharma, R. and Beg, Q.K. 2013. Revisiting microbial keratinases: next generation proteases for sustainable biotechnology. Critical Reviews in Biotechnology 33:216-228.
  19. Gupta, R. and Ramnani, P. 2006. Microbial keratinases and their prospective applications: an overview. Applied Microbiology and Biotechnology 70:21-33.
  20. Gurav, R., Nalavade, V., Aware, C., Vyavahare, G., Bhatia, S.K., Yang, Y.H., Bapat, V. and Jadhav, J. 2020. Microbial degradation of poultry feather biomass in a constructed bioreactor and application of hydrolysate as bioenhancer to vegetable crops. Environmental Science and Pollution Research 27:2027-2035.
  21. Gurav, R.G. and Jadhav, J.P. 2013. A novel source of biofertilizer from feather biomass for banana cultivation. Environmental Science and Pollution Research 20:4532-4539.
  22. Herzog, B., Overy, D.P., Haltli, B. and Kerr, R.G. 2016. Discovery of keratinases using bacteria isolated from marine environments. Systematic and Applied Microbiology 39:49-57.
  23. Hua-Jing, W.A.N.G., Liang-Huan, W.U., Min-Yan, W.A.N.G., Yuan-Hong, Z.H.U., Qin-Nan, T.A.O. and Zhang, F.S. 2007. Effects of amino acids replacing nitrate on growth, nitrate accumulation, and macroelement concentrations in pak-choi (Brassica chinensis L). Pedosphere 17:595-600.
  24. Jain, R., Jain, A., Rawat, N., Nair, M. and Gumashta, R. 2016. Feather hydrolysate from Streptomyces sampsonii GS 1322: A potential low cost soil amendment. Journal of Bioscience and Bioengineering 121:672-677.
  25. Jayalakshmi, T., Krishnamoorthy, P., Kumar, G.R., Sivamani, P. and Lakshmi, C.A. 2012. Application of pure keratinase on keratinous fibers to identify the keratinolytic activity. Journal Chem Pharm Research 4:3229-3233.
  26. Jayathilakan, K., Sultana, K., Radhakrishna, K. and Bawa, A.S. 2012. Utilization of byproducts and waste materials from meat, poultry and fish processing industries: a review. Journal of Food Science and Technology 49:278-293.
  27. Jiang, H., Dong, H., Zhang, G., Yu, B., Chapman, L.R. and Fields, M.W. 2006. Microbial diversity in water and sediment of Lake Chaka, an athalassohaline lake in northwestern China. Applied and Environmental Microbiology 72:3832-3845.
  28. Kim, J.M., Choi, Y.M. and Suh, H.J. 2005. Preparation of feather digests as fertilizer with Bacillus pumilis KHS-1. Journal of Microbiology and Biotechnology 15:472-476.
  29. Kshetri, P., Roy, S.S., Sharma, S.K., Singh, T.S., Ansari, M.A., Prakash, N. and Ngachan, S.V. 2019. Transforming chicken feather waste into feather protein hydrolysate using a newly isolated multifaceted keratinolytic bacterium Chryseobacterium sediminis RCM-SSR-7. Waste and Biomass Valorization 10:1-11.
  30. Liu, X.Q., Chen, H.Y., Ni, Q.X. and Kyu, S.L. 2008. Evaluation of the role of mixed amino acids in nitrate uptake and assimilation in leafy radish by using 15N-labeled nitrate. Agricultural Sciences in China 7:1196-1202.
  31. Mobini, M., Khoshgoftarmanesh, A.H. and Ghasemi, S. 2014. The effect of partial replacement of nitrate with arginine, histidine, and a mixture of amino acids extracted from blood powder on yield and nitrate accumulation in onion bulb. Scientia Horticulturae 176:232-237.
  32. Park, G.T. and Son, H.J. 2009. Keratinolytic activity of Bacillus megaterium F7-1, a feather-degrading mesophilic bacterium. Microbiological Research 164:478-485.
  33. Qiu, J., Wilkens, C., Barrett, K. and Meyer, A.S. 2020. Microbial enzymes catalyzing keratin degradation: Classification, structure, function. Biotechnology Advances, 44, p.107607.
  34. Rai, S.K. and Mukherjee, A.K. 2015. Optimization for production of liquid nitrogen fertilizer from the degradation of chicken feather by iron-oxide (Fe3O4) magnetic nanoparticles coupled β-keratinase. Biocatalysis and Agricultural Biotechnology 4:632-644.
  35. Ramnani, P., Singh, R. and Gupta, R. 2005. Keratinolytic potential of Bacillus licheniformis RG1: structural and biochemical mechanism of feather degradation. Canadian Journal of Microbiology 51:191-196.
  36. Reddy, N., Jiang, Q., Jin, E., Shi, Z., Hou, X. and Yang, Y. 2013. Bio-thermoplastics from grafted chicken feathers for potential biomedical applications. Colloids and Surfaces B: Biointerfaces 110:51-58.
  37. Reddy, N. and Yang, Y. 2005. Biofibers from agricultural byproducts for industrial applications. Trends in Biotechnology 23:22-27.
  38. Samuolienė, G., Urbonavičiūtė, A., Duchovskis, P., Bliznikas, Z., Vitta, P. and Žukauskas, A. 2009. Decrease in nitrate concentration in leafy vegetables under a solid-state illuminator. Hort Science 44:1857-1860.
  39. Singh, R., Kumar, M., Mittal, A. and Mehta, P.K. 2017. Microbial metabolites in nutrition, healthcare and agriculture. 3 Biotech, 7:1-14.
  40. Srivastava, A., Sharma, A. and Suneetha, V. 2011. Feather waste biodegradation as a source of amino acids. European Journal of Experimental Biology 1:56-63.
  41. Sun, Z., Li, X., Liu, K., Chi, X. and Liu, L. 2021. Optimization for Production of a Plant Growth Promoting Agent from the Degradation of Chicken Feather Using Keratinase Producing Novel Isolate Bacillus pumilus Waste and Biomass Valorization 12:1943-1954.
  42. Tiwary, E. and Gupta, R. 2012. Rapid conversion of chicken feather to feather meal using dimeric keratinase from Bacillus licheniformis ER-15. J Bioprocess Biotech 2: 123.
  43. Van Zinderen Bakker, E.M. 1986. Development of hydroponic systems and a look into the future. Section III. In Proceedings 7th Annual Conference on Hydroponics. Hydroponic Society of America, Concord, CA (pp. 73-74).
  44. Vasileva-Tonkova, E., Gousterova, A. and Neshev, G. 2009. Ecologically safe method for improved feather wastes biodegradation. International Biodeterioration and Biodegradation 63:1008-1012.
  45. Verma, A., Singh, H., Anwar, M.S., Kumar, S., Ansari, M.W. and Agrawal, S. 2016. Production of thermostable organic solvent tolerant keratinolytic protease from Thermoactinomyces RM4: IAA production and plant growth promotion. Frontiers in Microbiology 7:1189.
  46. Vernieri, P., Borghesi, E., Tognoni, F., Serra, G., Ferrante, A. and Piagessi, A. 2006, October. Use of biostimulants for reducing nutrient solution concentration in floating system. In III International Symposium on Models for Plant Growth, Environmental Control and Farm Management in Protected Cultivation 718:477-484.
  47. Williams, C.M., Richter, C.S., MacKenzie Jr, J.M. and Shih, J.C. 1990. Isolation, identification, and characterization of a feather-degrading bacterium. Applied and Environmental Microbiology 56:1509-1515.
آمار
تعداد مشاهده مقاله: 642
تعداد دریافت فایل اصل مقاله: 422


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب