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تأثیر قارچکش بنومیل و سیتوکینین بر میزان آلودگی و رشد ریزنمونههای گرهای Vaccinium arctostaphylos .L. در شرایط درون شیشهای | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 10، دوره 38، شماره 1 - شماره پیاپی 111، فروردین 1401، صفحه 150-165 اصل مقاله (843.07 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2022.356225.3070 | ||
| نویسندگان | ||
| مهران نوروزپور* 1؛ ناصر زارع2؛ رسول اصغری زکریا3؛ پریسا شیخ زاده4 | ||
| 1دانشجوی دکترای بیوتکنولوژی، گروه زراعت و اصلاح نباتات، دانشگاه محقق اردبیلی، اردبیل، ایران | ||
| 2دانشیار، گروه زراعت و اصلاح نباتات، دانشگاه محقق اردبیلی، اردبیل، ایران | ||
| 3استاد، گروه زراعت و اصلاح نباتات، دانشگاه محقق اردبیلی، اردبیل، ایران | ||
| 4استادیار، گروه زراعت و اصلاح نباتات، دانشگاه محقق اردبیلی، اردبیل، ایران | ||
| چکیده | ||
| آلودگی میکروبی یکی از چالشهای اصلی و از عوامل تأثیرگذار در پاسخ ریزنمونهها در کشتهای درون شیشهای است، بهویژه در گیاهان چوبی که ریزنمونهها از رویشگاه طبیعی تهیه میگردد. در تحقیق حاضر، جهت بررسی تأثیر بنومیل بر میزان آلودگی و رشد ریزنمونههای گره قرهقاط (Vaccinium arctostaphylos L.)، از محیط کشت MS حاوی غلظتهای مختلف بنومیل (0 تا2000 میلیگرم بر لیتر) استفاده شد. همچنین، جهت بررسی برهمکنش بنومیل، محیط کشت پایه و ترکیب هورمونی بر پاسخ رشدی ریزنمونهها، محیط کشتهای پایه مختلف (MS، AN و WPM) حاوی IBA (0.1 میلیگرم بر لیتر)، سیتوکینین (زآتین، BAP و TDZ در غلظتهای 0، 0.5، 1 و 2 میلیگرم بر لیتر) و بنومیل (صفر و 150 میلیگرم بر لیتر) بررسی شدند. نتایج نشان داد که استفاده از غلظتهای مختلف بنومیل در محیط کشت پایه MS تأثیر معنیداری بر میزان آلودگی ریزنمونهها نداشت، ولی استفاده از بنومیل در غلظتهای پایینتر (400 میلیگرم بر لیتر یا کمتر)، شرایط رشدی مناسبی را فراهم کرد. اثرمتقابل تیمارها تأثیر معنیداری بر درصد زندهمانی و برگدهی ریزنمونهها داشت. در محیط MS، درصد زندهمانی و برگدهی در حضور بنومیل بیشتر از محیط فاقد بنومیل بوده، ولی در محیطهای AN و WPM درصد زندهمانی و برگدهی بهطور معنیداری در حضور بنومیل کاهش پیدا کرد. علاوهبراین، در محیطهای AN و WPM حاوی سیتوکینین، حضور بنومیل باعث کاهش معنیدار درصد برگدهی شد، ولی در محیط MS بسته به نوع و غلظت سیتوکینین تأثیر بنومیل متفاوت بود. | ||
| کلیدواژهها | ||
| بنومیل؛ زآتین؛ کشت بافت گیاهی؛ BAP؛ TDZ | ||
| عنوان مقاله [English] | ||
| Effects of benomyl and cytokinin on in vitro contamination and growth of Vaccinium arctostaphylos L. node explants | ||
| نویسندگان [English] | ||
| m. Noruzpuor1؛ n. Zare2؛ R. Asghari Zakaria3؛ P. Sheikhzade4 | ||
| 1Ph.D. student, of Agricultural Biotechnology, Department of Agronomy and Plant Breeding, University of Mohaghegh Ardabili, Ardabil, Iran | ||
| 2Department of Agronomy and Plant Breeding, University of Mohaghegh Ardabili, Ardabil, Iran | ||
| 3Department of Agronomy and Plant Breeding, University of Mohaghegh Ardabili, Ardabil, Iran | ||
| 4Department of Agronomy and Plant Breeding, University of Mohaghegh Ardabili, Ardabil, Iran | ||
| چکیده [English] | ||
| Microbial contamination is one of the main challenges and influential factors in the response of explants under in vitro cultures, especially in woody plants where the explants are prepared from natural habitats. In the present study, to investigate the effects of benomyl on microbial contamination and growth of Vaccinium arctostaphylos L. node explants, MS containing different concentrations of benomyl (0-2000 mg.L-1) was used. Furthermore, to investigate the interaction of benomyl, basal medium, and hormonal composition on the growth response of explants, different basal media (MS, AN, and WPM) containing IBA (0.1 mg.L-1), cytokinin (Zeatin, BAP, and TDZ, each at concentrations of 0, 0.5, 1, and 2 mg.L-1), and benomyl (0 and 150 mg.L-1) were studied. The results showed that benomyl in MS medium had no significant effect on the explants contamination, but use of lower benomyl concentrations (400 mg.L-1 or less) could provide better growth conditions. Interaction of treatments significantly affected the percentage of survival and explants foliation. MS contained benomyl showed higher percentage of survival and foliation than MS without benomyl, but in AN and WPM media, the percentage of survival and foliation were significantly decreased in the presence of benomyl. Furthermore, in AN and WPM media contained cytokinin, the presence of benomyl significantly reduced the foliation percentage, while in MS medium, the effect of benomyl was different depending on the concentration and cytokinin type. | ||
| کلیدواژهها [English] | ||
| Benomyl, zeatin, plant tissue culture, BAP, TDZ | ||
| مراجع | ||
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