| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شمارهها | 3,094 |
| تعداد مقالات | 34,537 |
| تعداد مشاهده مقاله | 49,391,189 |
| تعداد دریافت فایل اصل مقاله | 79,840,482 |
تأثیر باکتریهای محرک رشد بر برخی صفات کمی و فیریولوژیک داغداغان (.Celtis caucasian Wild) تحت تنش سرب | ||
| تحقیقات جنگل و صنوبر ایران | ||
| مقاله 7، دوره 29، شماره 4 - شماره پیاپی 86، دی 1400، صفحه 377-390 اصل مقاله (307.31 K) | ||
| نوع مقاله: علمی- پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijfpr.2022.356936.2032 | ||
| نویسندگان | ||
| فاطمه مرادی1؛ میترا اعلائی* 2؛ مسعود ارغوانی3؛ فهیمه صالحی4 | ||
| 1دانشجوی کارشناسی ارشد گیاهان زینتی، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران | ||
| 2نویسنده مسئول، استادیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران | ||
| 3استادیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران | ||
| 4دانشجوی دکتری اصلاح و بیوتکنولوژی گیاهان باغبانی، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه زنجان، زنجان، ایران | ||
| چکیده | ||
| بهمنظور بررسی تأثیر باکتریهای محرک رشد تحت تنش سرب بر نهالهای داغداغان (Celtis caucasica Willd.)، پژوهش پیشرو بهصورت فاکتوریل در قالب طرح کامل تصادفی در شرایط گلخانه در زنجان اجرا شد. تیمار سرب شامل سه سطح صفر، 50 و 100 میلیگرم بر کیلوگرم و تیمار باکتریایی شامل شاهد (بدون باکتری)، سودوموناس پوتیدا (Pseudomonas putida) و باسیلوس سوبتیلیس (Bacillus subtilis) با دو غلظت 105 و 108 باکتری زنده در میلیلیتر بودند. برخی صفتهای مورفولوژیک و فیزیولوژیک شامل وزنهای تر و خشک ریشه، ارتفاع گیاه، کلروفیل کل، فنل کل، فلاونوئید، فعالیت آنزیمهای پراکسیداز و مالوندیآلدئید، غلظت فسفر در برگ و غلظت سرب در برگ و ریشه اندازهگیری شدند. بهمنظور بررسی تأثیر پارامترهای مذکور از تجزیه واریانس دوطرفه و مقایسه میانگین بهروش دانکن استفاده شد. یافتههای این پژوهش نشان داد که کاربرد دو باکتری مورد نظر سبب بهبود عملکرد داغداغان در شرایط آلودگی با سرب میشود. بهطوریکه اثرات متقابل سرب و باکتری بر وزنهای تر و خشک ریشه، ارتفاع گیاه، کلروفیل کل و فلاونوئید معنیدار بودند. بیشترین فعالیت پراکسیداز در تیمار 100 میلیگرم سرب بر کیلوگرم با غلظت 105 باسیلوس سوبتیلیس بر میلیلیتر مشاهده شد. در تیمار مذکور، ۵۳/۲۶ میلیگرم سرب بر گرم وزن تر ریشه بهدست آمد که بهطور معنیداری نسبت به تیمارهای دیگر بیشتر بود. همچنین، بیشینه جذب سرب توسط برگ (8/16 میلیگرم بر گرم وزن تر) در تیمار باسیلوس سوبتیلیس با غلظت 108 باکتری زنده بر میلیلیتر مشاهده شد. بهطورکلی، داغداغان در محیط آلوده به سرب، گیاهپالایی را بهخوبی انجام داد، اما صفتهای رشدی آن بهشدت کم شد. با کاربرد باکتریهای محرک رشد بهویژه باسیلوس سوبتیلیس میتوان از کاهش شدید رشد آن جلوگیری کرد. | ||
| کلیدواژهها | ||
| باسیلوس سوبتیلیس؛ پراکسیداز؛ سودوموناس پوتیدا؛ فسفر؛ گیاهپالایی | ||
| عنوان مقاله [English] | ||
| The effect of growth-promoting bacteria on some morphologic and physiological traits of Celtis caucasica Willd. under lead stress | ||
| نویسندگان [English] | ||
| F. Moradi1؛ M. Aelaei2؛ M. Arghavani3؛ F. Salehi4 | ||
| 1M.Sc. Student of Ornamental plants, Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran | ||
| 2Corresponding author, Assistant Prof, Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran E-mail: maelaei@znu.ac.ir | ||
| 3Assistant Prof, Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran | ||
| 4Ph.D. Candidate of breeding and biotechnology of horticultural plants, Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran | ||
| چکیده [English] | ||
| In order to investigate the effect of plant growth in soil pollution conditions in hackberry plant (Celtis caucasica Willd.), a test was carried out as a factorial design in a completely random pattern in the research conservatory of the University of Zanjan, Iran. The treatments included three levels of lead (0, 50, 100 mg/kg) and bacterial treatments invcluing Pseudomonas putida and Bacillus subtilis with concentrations of 105 and 108 cfu/ml. The attributes of the evaluation include root fresh and dry weights, plant height, total chlorophyll, total phenol, flavonoids and enzyme activity including peroxidase and malon-di-aldehyde, lead value in root and leaf and phosphorus in leaf. The two-way analysis of variance and compare the comparison with Duncan's method were used in order to investigate the effects of the lead stress and bacteria. The findings of this study showed that the use of bacteria in lead stress improved the growth and function of hackberry plant including root fresh and dry weights, plant height, total chlorophyll and flavonoids. The maximum amount of peroxidase was obtained in the treatment of 100 mg/kg lead and Bacillus subtilis (105 cfu/ml). In this treatment, the amount of lead in the fresh roots was 26.53 mg/g, which was a significant difference compared to the other treatments. Also, the highest amount of lead uptake by leaves (16.8 mg/g Fw) was observed under the treatment of Bacillus subtilis with a concentration of 108 cfu/ml. While hackberry plant performed well in terms of phytoremediation under lead stress, its growth characteristics were significantly diminished, but with the presence of growth-promoting bacteria specially Bacillus subtilis that can prevent severe reduction. | ||
| کلیدواژهها [English] | ||
| Bacillus subtilis, peroxidase, phosphorus, phytoremediation, Pseudomonas putida | ||
| مراجع | ||
|
- Aalipour, H., Nikbakht, A., Etemadi, N., Soleimani, M. and Rejali, F., 2019. Evaluating the effect of cadmium on the decline of Arizona cypress seedlings and the enhancement role of mycorrhizal fungus and plant growth promoting rhizobacteria. Journal of Water and Soil Science (Science and Technology of Agriculture and Natural Resources), 23(2): 417-431 (In Persian). - Ali, M.B., Vajpayee, P., Tripathi, R.D., Rai, U.N., Singh, S.N. and Singh, S.P., 2003. Phytoremediation of lead, nickel, and copper by Salix acmophylla Boiss.: Role of antioxidant enzymes and antioxidant substances. Bulletin of Environmental Contamination and Toxicology, 70: 462-469. - Arnon, D.I., 1996. Copper enzymes in isolated chloroplasts. Polyphenol oxidase in beta vulgaris. Plant Physiology, 24: 1 -15. - Azampoor, S., Pilehvar, B., Shirvany, A., Bayramzadeh, V. and Ahmadi, M., 2013. Nickle phytoremediation by leaves of planted species (Fraxinus rotundifolia, Ulmus densa, Salix alba) (Case study: Kermanshah oil refinery area). Iranian Journal of Forest, 5(2): 141-150 (In Persian). - Canbolat, M.Y., Bilen, S., Çakmakçı, R., Şahin, F. and Aydın, A., 2006. Effect of plant growth-promoting bacteria and soil compaction on barley seedling growth, nutrient uptake, soil properties and rhizosphere microflora. Biology and Fertility of Soils, 42(4): 350-357. - Chang, C.C., Yang, M.H., Wen, H.M. and Chern, J.C., 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis, 10(3): 178-182. - Dehnavi, S., Matinkhah, H. and Nourbakhsh, F., 2014. The role of Hackberry "Celtis caucasica" as nitrogen-fixing trees on understory’s soil properties in reserved area in Ardasteh-Dehaghan in Isfahan. Iranian Journal of Forest and Poplar Research, 21(4): 643-653 (In Persian). - Dell’Amico, E., Cavalca, L. and Andreoni, V., 2008. Improvement of Brassica napus growth under cadmium stress by cadmium-resistant rhizobacteria. Soil Biology and Biochemistry, 40(1): 74-84. - Demir, S., 2004. Influence of arbuscular mycorrhiza on some physiological growth parameters of pepper. Turkish Journal of Biology, 28: 85-90. - Dhindsa, R.S., Plumb-Dhindsa, P. and Thorpe, T.A., 1981. Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. Journal of Experimental Botany, 32(1): 93-101. - Dickinson, N.M. and Pulford, I.D., 2005. Cadmium phytoextraction using short-rotation coppice Salix: the evidence trail. Environment International, 31(4): 609-613. - Fageria, N.K., Moreira, A. and Dos Santos, A.B., 2013. Phosphorus uptake and use efficiency in field crops. Journal of Plant Nutrition, 36: 2013-2022. - Fu, J., Zhou, Q., Liu, J., Liu, W., Wang, T., Zhang, Q. and Jiang, G., 2008. High levels of heavy metals in rice (Oryza sativa L.) from a typical E-waste recycling area in southeast China and its potential risk to human health. Chemosphere, 71: 1269-1275. - Glick, B.R., 2003. Phytoremediation: Synergistic use of plants and bacteria to clean up the environment. Biotechnology Advances, 21(5): 383-393. - Glick, B.R., 2010. Using soil bacteria to facilitate phytoremediation. Biotechnology Advances, 28(3): 367-374. - Gupta, P.K., 2009. Soil, Plant, Water and Fertilizer Analysis. Agrobios, Jodhpur, 366p. - Heath, R.L. and Packer, L., 1968. Photo peroxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Biochem. Biophys, 125(1): 189-98. - Hong, L., Li, M., Luo, J., Cao, X., Qu, L., Gai, Y., ... and Luo, Z.B., 2012. N-fertilization has different effects on the growth, carbon and nitrogen physiology, and wood properties of slow- and fast-growing Populus species. Journal of Experimental Botany, 63(17): 6173-6185. - Khalighi Jamal-Abad, A. and Khara, J., 2008. The effect of arbuscular mycorrhizal fungus Glomus intraradices on some growth and physiological parameters in wheat (CV. Azar2) plants under cadmium toxicity. Iranian Journal of Biology, 21(2): 216-230 (In Persian). - Khan, M.S., Zaidi, A., Wani, P.A. and Over, M., 2009. Role of plant growth promoting rhizobacteria in the remediation of metal contaminated soils. Environmental Chemistry Letters, 7: 1-19. - Khatamsaz, M., 1990. Flora of Iran. No. 4: Ulmaceae. Published by Research Institute of Forests and Rangelands, Tehran, 28p (In Persian). - Kováčik, J., Klejdus, B., Hedbavny, J., Covska, S. and Zoń, J., 2011. Significance of phenols in cadmium and nickel uptake. Journal of Plant Physiology, 168(6): 576-584. - Marinova, D., Ribarova, F. and Atanassova, M., 2005. Total phenolics and total flavonoids in Bulgarian fruits and vegetables. Journal of the University of Chemical Technology and Metallurgy, 40(3): 255-260. - Matyar, F., Kaya, A. and Dinçer, S., 2008. Antibacterial agents and heavy metal resistance in Gram-negative bacteria isolated from seawater, shrimp and sediment in Iskenderun Bay, Turkey. Science of The Total Environment, 407(1): 279-285. - Mishra, V., Gupta, A., Kaur, P., Singh, S., Singh, N., Gehlot, P. and Singh, J., 2016. Synergistic effects of Arbuscular mycorrhizal fungi and plant growth promoting rhizobacteria in bioremediation of iron contaminated soils. International Journal of Phytoremediation, 18(7): 697-703. - Olsen, S.R., Cole, C.V., Watanabe, F.S. and Dean, L.A., 1954. Estimation of available phosphorus insoils by extraction with sodium bicarbonate. USDA Circular. Government. Printing Office. Washington D.C., 939(1): 1-19. - Remacle, J., 1990. The cell wall and metal binding: 83-92. In: Volesky, B. (Ed.). Biosorption of Heavy Metals. CRC Press, Boca Raton, Florida, 408p. - Rostamikia, Y., Tabari Kouchaksaraei, M., Asgharzadeh, A. and Rahmani, A., 2017. Effect of growth promoting rhizobacteria on growth and nutrient elements of common hazelnut (Corylus avellana L.) seedlings in Ardabil Fandoqlou nursery. Iranian Journal of Forest and Poplar Research, 25(1): 116-126 (In Persian). - Salehi, A., Tabari, M. and Shirvani, A., 2014. Survival, growth and Pb concentration of Populus alba (clone 44/9) seedling in Pb-contaminated soil. Iranian Journal of Forest, 6(4): 419-433 (In Persian). - Shen, Z.G., Li, X.D., Wang, C.C., Chen, H.M. and Chua, H., 2002. Lead phytoextraction from contaminated soil with high-biomass plant species. Journal of Environmental Quality, 31(6): 1893-1900. - Vijayaraghavan, K. and Yun, Y.S., 2008. Bacterial biosorbents and biosorption. Biotechnology Advances, 26(3): 266-291. - Walker, M.A. and McKersie, B.D., 1993. Role of the ascorbate-glutathione antioxidant system in chilling resistance of tomato. Journal of Plant Physiology, 141(2): 234-239. - Yang, S., Liang, S., Yi, L., Xu, B., Cao, J., Guo, Y. and Zhou, Y., 2014. Heavy metal accumulation and phytostabilization potential of dominant plant species growing on manganese mine tailings. Frontiers of Environmental Science and Engineering, 8(3), 394-404. - Zare, A.A., Malakouti, M.J., Bahrami, H.A. and Sefidkon, F., 2018. Effect of bio fertilizers on the yield and essential oil composition of Lemon Verbena (Lippia citriodora). Horticultural Plants Nutrition, 1(1): 29-40 (In Persian). | ||
|
آمار تعداد مشاهده مقاله: 657 تعداد دریافت فایل اصل مقاله: 515 |
||