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moharrami, M., Ahmadi Niri, E. (2022). Investigation of genotypic diversity of Paenibacillus larvae strains isolated from Iranian honey bee colonies.. , 35(4), 91-97. doi: 10.22092/vj.2022.357516.1935
M moharrami; E Ahmadi Niri. "Investigation of genotypic diversity of Paenibacillus larvae strains isolated from Iranian honey bee colonies.". , 35, 4, 2022, 91-97. doi: 10.22092/vj.2022.357516.1935
moharrami, M., Ahmadi Niri, E. (2022). 'Investigation of genotypic diversity of Paenibacillus larvae strains isolated from Iranian honey bee colonies.', , 35(4), pp. 91-97. doi: 10.22092/vj.2022.357516.1935
moharrami, M., Ahmadi Niri, E. Investigation of genotypic diversity of Paenibacillus larvae strains isolated from Iranian honey bee colonies.. , 2022; 35(4): 91-97. doi: 10.22092/vj.2022.357516.1935

Investigation of genotypic diversity of Paenibacillus larvae strains isolated from Iranian honey bee colonies.

تحقیقات دامپزشکی و فرآورده‌های بیولوژیک
Article 11, Volume 35, Issue 4, December 2022, Pages 91-97    PDF (1.01 M)
Document Type: Full Research Paper
DOI: 10.22092/vj.2022.357516.1935
Authors
M moharrami* 1; E Ahmadi Niri2
1Department of Honey Bee, Silk Worm a Wildlife, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran
2Department of Microbial Biotechnology, Faculty of Basic Sciences, University of Science and Culture, Tehran, Iran
Abstract
American Foulbrood disease is a deadly bacterial disease of honey bee larvae in the world. The disease is caused by gram-positive and spores forming bacteria of Paenibacillus larvae that has significant economic losses. However, no study has been done on the diversity of this disease agent in Iran. In this study, 19 strains of Penibacillus larvae that were isolated from diseased and dead larvae and honey after confirmation by biochemical tests, PCR of 16S rRNA gene and sequencing, were used in ERIC-PCR technique to study genotypic diversity. The results enabled the identification of ERIC I and ERIC II genotypes for the first time in Iran.  14 strains of genotype II (73.7%) and 5 strains showed genotype I (26.3%).
Keywords
Paenibacillus larvae; American Foulbrood; (ERIC-PCR) technique; ERIC genotypes
References

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