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اثر پرایمینگ بذر در افزایش بنیه بذر، رشد گیاهجه و فعالیت‌های آنزیمی در بذور زوال یافته 4 گونه جنس اسپرس Onobrychis sp | ||
| علوم و فناوری بذر ایران | ||
| مقاله 3، دوره 10، شماره 4 - شماره پیاپی 24، آذر 1400، صفحه 33-55 اصل مقاله (1.31 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijsst.2021.351587.1359 | ||
| نویسندگان | ||
| آزاده کاوندی1؛ علی اشرف جعفری* 2؛ امیر قربانحانی1؛ فرشته شاهبازی اصل3 | ||
| 1پژوهشگر، مؤسسه تحقیقات جنگل ها و مراتع کشور ، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| 2استاد پژوهش موسسه تحقیقات جنگل ها و مراتع کشور | ||
| 3پژوهشگر، مؤسسه تحقیقات ثبت و گواهی بذر و نهال ، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| چکیده | ||
| زوال بذر یکی از عوامل کاهش‌دهنده بنیه و محدودکننده جوانه‌زنی است. به‌منظور بررسی اسموپرایمینگ بر کاهش اثرات زوال بذور در 4 گونه از جنس اسپرس Onobrychis، آزمایش فاکتوریل درقالب طرح کاملا تصادفی در 4 تکرار در گلخانه‌ای موسسه تحقیقات جنگل ها و مراتع کشور به اجرا درآمد. گونه‌های مورد استفاده شامل (O.cristagalli، O.micoxchi، O.sintensii و O.vicifolia) بودند. بذرها در معرض دمای °C41 + و رطوبت 100% در سه بازه زمانی (صفر)، 48 ساعت و 72 ساعت در انکوباتور قرار گرفتند. پس از آزمون پیری‌زودرس ، اثر غلظت‌های صفر و 6/0- و 2/1-) مگاپاسکال پلی‌اتیلن گلایکول بر بازیافت بذرهای زوال یافته مورد بررسی قرار گرفت و صفات مرتبط با جوانه زنی بذر اندازه‌گیری شد. نتایج نشان داد که زوال بذر سبب کاهش صفات جوانه‌زنی در همه گونه‌ها شد. اثر متقابل پرایمینگ در زوال بذر برای اکثر صفات معنی‌دار بود و در گونه O.vicifolia میانگین صفات بذرهای زوال یافته نسبت به سایر گونه‌ها بیشتر بود. نتایج نشان داد که اسموپرایمینگ سبب بهبود میانگین اکثر صفات شد. اثر مثبت غلظت‌های بالاتر اسموپرایمینگ در افزایش طول ریشه و ساقه بذرهای پیر شده چشم‌گیر بود. در ادامه با نمونه‌گیری از گیاهان کشت شده در گلخانه فعالیت آنزیم‌های آنتی‌اکسیدانت کاتالاز و پراکسیداز و دیسموتاز در گونه‌های اسپرس بررسی شد. نتایج نشان داد که زوال بذر سبب کاهش فعالیت آنزیم‌های پراکسیداز و کاتالاز شد. بذرهای زوال یافته که با PEG پرایم شده بودند دارای فعالیت آنزیمی بیشتری بودند. نتیجه‌گیری شد که کاربرد اسموپرایمینگ سبب افزایش ویژگی‌های اندازه‌گیری شده در بذرهای پیر شده اسپرس می‌شوند. | ||
| کلیدواژه‌ها | ||
| پیری‌زودرس؛ جوانه‌زنی؛ اسموپرایمینگ آنزیم‌های آنتی‌اکسیدانت | ||
| عنوان مقاله [English] | ||
| Effect of seed priming on enhancement of seed vigor, seedling growth and enzyme activities in accelerated aged seeds of four Sainfoin species | ||
| نویسندگان [English] | ||
| Azadeh kavandi1؛ Amir Ghorbankhani1؛ Fereshta shahbazi Asl3؛ | ||
| 1Research Expert, Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran | ||
| 3Research Expert, Seed and Plant Certification and Registration Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran | ||
| چکیده [English] | ||
| Seed deterioration is one of the factors reducing seed viability and vigor. In order to investigate the effect of osmopriming on seed vigor enhancement and seedling enzyme activities, a factorial experiment was conducted in a completely randomized design with four replications in greenhouse of Research Institute of Forests and Rangelands, Tehran, Iran in 2015. Experimental factors were four sainfoin species as (Onobrichis cristagalli, O. micoxchi, O. sintensii and O. vicifolia), three levels of deteriorated seeds using accelerated ageing techniques (40°C, 98% of RH for 48 and 72h and control) and three priming treatments including hydropriming (control) and osmopriming (PEG -0.6Mpa and -1.2 Mpa). Data were collected for germination percent, rate of germination, root length, shoot length, vigor index, seedling weight and the variability in the activities of three antioxidant enzymes as superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD). Results showed that seed deterioration decreased germination traits in all of sainfoin species. Priming by seed aging interaction effect was significant for most of traits. The O.vicifolia had higher mean values for germination traits in deteriorated seeds. The result indicated that application of PEG (-0.6Mpa and -1.2 Mpa) had improved seedling growth as root and shoot length in all of the species. Measurement of enzymatic activity showed that the activities of antioxidant enzymes were decreasing in aged seeds of sainfoin. However, application of PEG (-0.6Mpa) had improved enzymatic activity in aged seeds. It was concluded that osmopriming may enhance seed vigor, seedling growth and enzyme activities in deteriorated seed of sainfoin | ||
| کلیدواژه‌ها [English] | ||
| Aging test, Germination, Osmopriming antioxidant enzymes | ||
| مراجع | ||
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