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اثر پرایمینگ بذر در افزایش بنیه بذر، رشد گیاهجه و فعالیت‌های آنزیمی در بذور زوال یافته 4 گونه جنس اسپرس Onobrychis sp | ||
| علوم و فناوری بذر ایران | ||
| مقاله 3، دوره 10، شماره 4 - شماره پیاپی 24، آذر 1400، صفحه 33-55 اصل مقاله (1.31 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijsst.2021.351587.1359 | ||
| نویسندگان | ||
| آزاده کاوندی1؛ علی اشرف جعفری* 2؛ امیر قربانحانی1؛ فرشته شاهبازی اصل3 | ||
| 1پژوهشگر، مؤسسه تحقیقات جنگل ها و مراتع کشور ، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| 2استاد پژوهش موسسه تحقیقات جنگل ها و مراتع کشور | ||
| 3پژوهشگر، مؤسسه تحقیقات ثبت و گواهی بذر و نهال ، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| چکیده | ||
| زوال بذر یکی از عوامل کاهش‌دهنده بنیه و محدودکننده جوانه‌زنی است. به‌منظور بررسی اسموپرایمینگ بر کاهش اثرات زوال بذور در 4 گونه از جنس اسپرس Onobrychis، آزمایش فاکتوریل درقالب طرح کاملا تصادفی در 4 تکرار در گلخانه‌ای موسسه تحقیقات جنگل ها و مراتع کشور به اجرا درآمد. گونه‌های مورد استفاده شامل (O.cristagalli، O.micoxchi، O.sintensii و O.vicifolia) بودند. بذرها در معرض دمای °C41 + و رطوبت 100% در سه بازه زمانی (صفر)، 48 ساعت و 72 ساعت در انکوباتور قرار گرفتند. پس از آزمون پیری‌زودرس ، اثر غلظت‌های صفر و 6/0- و 2/1-) مگاپاسکال پلی‌اتیلن گلایکول بر بازیافت بذرهای زوال یافته مورد بررسی قرار گرفت و صفات مرتبط با جوانه زنی بذر اندازه‌گیری شد. نتایج نشان داد که زوال بذر سبب کاهش صفات جوانه‌زنی در همه گونه‌ها شد. اثر متقابل پرایمینگ در زوال بذر برای اکثر صفات معنی‌دار بود و در گونه O.vicifolia میانگین صفات بذرهای زوال یافته نسبت به سایر گونه‌ها بیشتر بود. نتایج نشان داد که اسموپرایمینگ سبب بهبود میانگین اکثر صفات شد. اثر مثبت غلظت‌های بالاتر اسموپرایمینگ در افزایش طول ریشه و ساقه بذرهای پیر شده چشم‌گیر بود. در ادامه با نمونه‌گیری از گیاهان کشت شده در گلخانه فعالیت آنزیم‌های آنتی‌اکسیدانت کاتالاز و پراکسیداز و دیسموتاز در گونه‌های اسپرس بررسی شد. نتایج نشان داد که زوال بذر سبب کاهش فعالیت آنزیم‌های پراکسیداز و کاتالاز شد. بذرهای زوال یافته که با PEG پرایم شده بودند دارای فعالیت آنزیمی بیشتری بودند. نتیجه‌گیری شد که کاربرد اسموپرایمینگ سبب افزایش ویژگی‌های اندازه‌گیری شده در بذرهای پیر شده اسپرس می‌شوند. | ||
| کلیدواژه‌ها | ||
| پیری‌زودرس؛ جوانه‌زنی؛ اسموپرایمینگ آنزیم‌های آنتی‌اکسیدانت | ||
| عنوان مقاله [English] | ||
| Effect of seed priming on enhancement of seed vigor, seedling growth and enzyme activities in accelerated aged seeds of four Sainfoin species | ||
| نویسندگان [English] | ||
| Azadeh kavandi1؛ Amir Ghorbankhani1؛ Fereshta shahbazi Asl3؛ | ||
| 1Research Expert, Research Institute of Forests and Rangelands, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran | ||
| 3Research Expert, Seed and Plant Certification and Registration Institute, Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran | ||
| چکیده [English] | ||
| Seed deterioration is one of the factors reducing seed viability and vigor. In order to investigate the effect of osmopriming on seed vigor enhancement and seedling enzyme activities, a factorial experiment was conducted in a completely randomized design with four replications in greenhouse of Research Institute of Forests and Rangelands, Tehran, Iran in 2015. Experimental factors were four sainfoin species as (Onobrichis cristagalli, O. micoxchi, O. sintensii and O. vicifolia), three levels of deteriorated seeds using accelerated ageing techniques (40°C, 98% of RH for 48 and 72h and control) and three priming treatments including hydropriming (control) and osmopriming (PEG -0.6Mpa and -1.2 Mpa). Data were collected for germination percent, rate of germination, root length, shoot length, vigor index, seedling weight and the variability in the activities of three antioxidant enzymes as superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD). Results showed that seed deterioration decreased germination traits in all of sainfoin species. Priming by seed aging interaction effect was significant for most of traits. The O.vicifolia had higher mean values for germination traits in deteriorated seeds. The result indicated that application of PEG (-0.6Mpa and -1.2 Mpa) had improved seedling growth as root and shoot length in all of the species. Measurement of enzymatic activity showed that the activities of antioxidant enzymes were decreasing in aged seeds of sainfoin. However, application of PEG (-0.6Mpa) had improved enzymatic activity in aged seeds. It was concluded that osmopriming may enhance seed vigor, seedling growth and enzyme activities in deteriorated seed of sainfoin | ||
| کلیدواژه‌ها [English] | ||
| Aging test, Germination, Osmopriming antioxidant enzymes | ||
| مراجع | ||
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Abdul Baki, A.A., and J.D. Anderson. 1973. Relationship between decarboxylation of glutamic acid and vigor in soybean seed. Crop Sci. 13: 227–232. Aebi H. 1984. Catalase in vitro. Methods Enzymol. 105:121–126. Amooaghaie, R. 2011. The effect of hydro and osmopriming on alfalfa seed germination and antioxidant defenses under salt stress. Afr. J. Biotechnol. 10: 6269-6275 Artola, A., G. Carrillo-Castaneda, and G.D.L. Santos. 2003. Hydropriming: A Strategy to increase Lotus corniculatus L. seed vigor. Seed Sci. Technol. 31(2): 455-463. Ashkani, JH., Pakniyat, Y. Emam, M. Assad, and M. Bahrani. 2007. The evaluation and relationships of some physiological traits in spring safflower (Carthamus tinctorius L.). J. Agric. Sci. 9(4), 267-277. Ashraf, M., and M.R. Foolad. 2005. Pre-sowing seed treatment: A shotgun approach to improve germination, plant growth and crop yield under saline and non-saline conditions. Adv. Agron. 88: 223-271. Rastegar, Z., M. Sedghi, and S. Khomari. 2011. Effects of Accelerated aging on soybean seed germination indexes at laboratory condition. Notulae Sci. Biol. 3(3):126-129. Azadi, M. S., S. A Tabatabaei, E.Younesi, M. R. Rostami, and M. Mombeni. 2013. Hormone priming improve germination characteristics and enzyme activity of sorghum Seeds (Sorghum bicolor L.) under accelerated aging. Cercetari Agronomice in Moldova. 3(155): 49-56. Azooz, M.M. 2009. Salt stress mitigation by seed priming with salicylic acid in two faba bean genotypes differing in salt tolerance. Int. J. Agric. Biol. 11:343-350. Baladi, S., H. Balouchi, A. Moradi, and M. Movahedi Dehnavi. 2016. The Effect of different temperatures and moisture during storage period on germination indices of Linum usitatissimum L. Iranian J. Seed Sci.Tech. 5(1): 107-122. (In Persian, with English Abstract) Baghaie-Nia, M., M. M. Majidi, and A. Mirlohi. 2010. Effects of induced mutation on general combining ability and association of traits in Sainfoin (Onobrychis viciifolia Scop.). Iranian J. Rang. For. Plant Breed Genet. Res. 18 (2): 181-198 (In Persian) Bailly, C., H. El-Maarouf-Bouteau, and F. Corbineau 2008. From intracellular signaling network to cell death: the dual role of reactive oxygen species in seed physiology. C.R. Biol. 331:806-814. Bam, R.k., F. K. Kumaga, K. Ofori, and E.A. Asiedu. 2006. Germination vigor and dehydrogenate activity of naturally aged Rice (Oryza Sativa L.) Seed soaked in potassium and phosphorus Salts. Asian J. Plant Sci. 5:948-955. Basra, S.M.A., N. Ahmad, M.M. Khan, N. Iqbal, and M.A. Cheema. 2003. Assessment of cotton seed deterioration during accelerated ageing. Seed Sci. Technol.31:531-540. Bernal, L., A. Camacho, and A. Caballo. 2000. Effect of seed aging in the enzymic antioxidant system of maize cultivars. PP. 151-160. In: M. Black, K.J. Bradford, and J. Vazqez-Ramos. Seed biology: advances and applications (eds). CABI publishing, UK. Bittebcourt, M.L.C., D.C.F.S. Dais., L.A.S. Dias, and E.F. Araujo. 2004. Effect of priming on asparagus seed germination and vigor under water and temperature stress. Seed Sci. Technol. 32: 607-616. Blokhina, O., E. Virolainen, and K.V. Fagerstedt. 2003. Antioxidants, oxidative damage and oxygen deprivation, stress: A review. Ann. Bot. 91: 179-194. Burguieres, E., P. McCu, Y. Kwon, and K. Shetty. 2007. Effect of vitamin C and folic acid on seed vigor response and phenolic-linked antioxidant activity. Bioresour. Technol. 98: 1393-1404. Cheraghi, F., S. Mahmoodi, M. Jami, and S. Parsa. 2012. Seed germination and growth improvement in Heracleum persicum Dest. by osmopriming. J. Herbal Drugs. 2(4): 229-238. Eisvand, H. R., R.Tavakol-Afshari, F. Sharifzadeh, and H. Maddah Arefi. 2010. How hormonal priming of aged and non-aged seeds of brome grass affects seedling physiological characters. J. New. Seed. 11:52-64. Farooq, M., S.M.A. Basra, R. Tabassum, and I. Afzal. 2006. Enhancing the performance of direct seeded, fine rice by seed priming. Plant Prod. Sci. 9: 446-456. Freitas, R.A, D.C.F.S Dias, G.A. Oliveira, L.A.S Dias, and I.C. José. 2006. Physiological and biochemical changes in naturally and artificially aged cotton seeds. Seed Sci. Technol. 34:253-264. Ghanavati, F., and H. Amirabadizadeh. 2012. Eco-geographical distribution of perennial species of onobrychis in Khorasan-e-Razavi Province. Seed Plant. Prod. 28(1): 19-34. (In Persian) Giannopolitis, CN, and SK. Ries 1977. Superoxide dismutase. I. Occurrence in higher plants, J. Plant Physiol. 59: 309–314. Giri, G. S., and W. F. Schillinger. 2003. Seed priming winter wheat for germination, emergence and yield. Crop Sci. 43: 2135-2141. Goel, A. K., and I.S. Sheoran. 2003. Changes in oxidative stress enzyme during artificial aging in cotton (Gossypium hirsutum L.) SED J. Plant Physiol. 160: 1093-1100. Jie, L. L. Ong She, O. Dong Mei, L. Fang, and W. Hua En. 2002. Effect of PEG on germination and active oxygen metabolism in wild rye (Elymus chinesis) seed. Acta Prataculture Sinica. 11:59-64. Kalpana, R., and K.V. Madhava-Rao. 1996. Lipid changes during accelerated ageing of seeds of pigeon pea (Cajanus cajan (L.) Millsp) cultivars. Seed Sci. Technol. 24: 475-483 Khan, A.A. 1992. Preplant physiological seed conditioning. Hortic. Rev. 14: 131-181. MacDonald, M. B. 1999. Seed deterioration: pHysiology, repair and assessment. Seed Sci. Technol. 27:177-237. MacDonald, M.B. 2000. Seed Priming. Pp 287-325. In: M. Black, and J.D. Bewley(eds). Seed technology and its biological basis. Sheffield Academic Press, Sheffield, UK. Maguire, J. D. 1962. Speed of germination, aid in selection and evaluation for seedling emergence and vigour. Crop Sci. 2: 176-177. Makkizadeh Tafti. M, R., Tavakol Afshari, N. Majnoon Hosseini, H.A. Naghdi Badi, and A. Mehdizadeh. 2006. Effect of osmopriming on seed germination of borage (Borago officinalis L.). Iranian J. Med. Arom. Plant. 22(3): 216-222 (In Persian) Matsushima, K.I., and J.I. Sakagami. 2013. Effects of seed hydropriming on germination and seedling vigor during emergence of rice under different soil moisture conditions. Am. J. Plant Sci. 4(8): 1584-1593. Marshal, A.H., and D.N. Lewis. 2004. Influence of seed storage conditions on seedling emergence, seedling growth and dry matter production of temperate forage grasses. Seed Sci. Technol. 32(2): 493- 501. Michel, B.E., and M.R. Kaufmann. 1973. The osmotic potential of polyethylene glycol 6000. Plant Physiol. 51: 914-916. Mozaffari, J., and M. R. Abbasi. 2005. Forage plants genetic resources in the national plant gene bank of Iran. The first National Conference of Forage Plants, Karaj, Iran. Oskouei, B., and S. Sheidaei. 2013. Study of different kinds of seed packaging and storage durations on seed vigor of canola using electrical conductivity test. Int. J. Agric. Crop Sci. 5 (5): 538-543. Pukacka, S., and E. Ratajczak. 2005. Production and scavenging of reaction oxygen species in Fagus sylvatica seeds during storage at varied temperature and humidity. J. Plant Physiol. 162: 873-885. Rassam, G., S., Rahban, M. Mojtabaii, and A. Badri. 2015. Effect of seed aging on germination and seedling growth of sunflower (Helianthus annuus) Cultivars. Iranian J. Seed Res. 1(2): 115-123. (In Persian) Schimtz, N., J.H. Xia, and A.R. Kermode. 2001. Dormancy of yellow cedar seeds is terminated by gibberellic acid in combination with fluridone or with osmotic priming and moist chilling. Seed Sci. Technol. 29: 331 -346. Simic, B., R. Popoviae, A. Sudaric, V. Rozman, I. Kalinovic, and J. Cosie. 2007. Influence of storage condition on seed oil content of maize, soybean and Sunflower. Agric. Conspectus. Sci. 72(3):211-213. Shannon, L M., E. Kay, and J. Y. Lew. 1966. Peroxidase isozymes from horseradish roots. I. Isolation and physical properties. J. Biol. Chem. 241(9):2166-2172. Sharp, R.E. 2002. Interaction with ethylene: changing views on therole of abscisic acid in root and shoot growth responses to water stress. Plant Cell Environ. 25: 211-222. Sheidaei, S., H. Heidari Sharisabad, A. Hamidi, G. Noormohammadi, and A. Moghaddam. 2016. Effect of storage condition, initial seed moisture content and germination on soybean seed deterioration. Iranian J. Seed Res. 2(2): 31-47 (In Persian) Tammela, P., P.S Vaananen, I. Lakso, A. Hopia, H. Voureia, and M. Nygrem. 2005. Tocopherolas, tocotrienols and fatty acids as indicators of natural aging in Pinus sylvestris Seeds. Scand. J. For. Res. 20:378-384. Tian, X., S.Song, and Y. Lei. 2008. Cell death and reactive oxygen species metabolism during accelerated ageing of soybean axes. Russ. J. Plant Physiol. 55: 33-40 Varier, A., A. Kuriakose, and M. Dadlani. 2010. The sub cellular basis of seed priming. Curr. Sci. 99 (4): 450-456. Walters, C., D. Ballesteros, and V.A. Vertucci. 2010. Structural mechanics of Seed deterioration: Standing the test of time. Plant Sci. 179: 565-573. Yang XH, Y.G. Wang, Y.W. X, H.F. Wang, and J.H. Ma. 2009. Effect of seed priming on the physiological characteristics of soybean seedling under water stress. Chinese J Eco-Agric. 17:1191-1195. Yoti, H., and C.P. Malik. 2013. Seed deterioration: A Review. Int. J. Life Sci. Biotechnol pharma Res. 2(3): 374-385. Zarnoushe Farahani. M., A.A. Jafari, and M.A. Alizadeh. 2019. Effect of Osmo priming, hormonal priming and hydropriming on the enhancement of aged seed germination and seedling growth of tansey (Tanacetum polycephalum). Iranian J. Seed Sci. Res. 6(2): 257-267. (In Persian) | ||
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