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jalali, G., Lakzian, A., Astaraei, A., Mazhari, M. (2022). Comparison of HRM and DGGE methods to study the composition of soil bacterial communities indifferent land uses. , 10(1), 49-64. doi: 10.22092/sbj.2022.354084.215
ghobad jalali; Amir Lakzian; Alireza Astaraei; Mahboobe Mazhari. "Comparison of HRM and DGGE methods to study the composition of soil bacterial communities indifferent land uses". , 10, 1, 2022, 49-64. doi: 10.22092/sbj.2022.354084.215
jalali, G., Lakzian, A., Astaraei, A., Mazhari, M. (2022). 'Comparison of HRM and DGGE methods to study the composition of soil bacterial communities indifferent land uses', , 10(1), pp. 49-64. doi: 10.22092/sbj.2022.354084.215
jalali, G., Lakzian, A., Astaraei, A., Mazhari, M. Comparison of HRM and DGGE methods to study the composition of soil bacterial communities indifferent land uses. , 2022; 10(1): 49-64. doi: 10.22092/sbj.2022.354084.215

Comparison of HRM and DGGE methods to study the composition of soil bacterial communities indifferent land uses

زیست شناسی خاک
Article 4, Volume 10, Issue 1, March 2022, Pages 49-64    PDF (584.05 K)
Document Type: Research Paper
DOI: 10.22092/sbj.2022.354084.215
Authors
ghobad jalali* 1; Amir Lakzian2; Alireza Astaraei3; Mahboobe Mazhari4
1Assistant Professor, Department of Soil Science, University of Jiroft
2Professor, Department of Soil Science, Ferdowsi University of Mashhad
3Associate Professor, Department of Soil Science, Ferdowsi University of Mashhad
4Assistant Professor, Faculty of Agriculture and Natural Resources, Karaj Islamic Azad University
Abstract
Land use change is one of the most important factors influencing soil microbial communities which play a pivotal role in most biogeochemical and ecological processes. In order to determine the effect of land use on the composition of soil bacterial communities, High Resolution Melt (HRM) analysis and Denaturing Gradient Gel Electrophoresis (DGGE) were used in three different land uses (orchard land, farmland and shrub land) in Jiroft plain, Iran. In the case of HRM analysis, a real-time PCR device equipped with this technique was used. DGGE technique via DCodeTM Universal Mutation Detection System was conducted to evaluate the composition of soil bacterial communities. The results of HRM as well as the ordination results based on the presence or absence of DGGE bands in soil samples using non-metric multidimensional scaling (NMDS) method showed that the land-use change from shrub land to agriculture (orchard and farm) had a significant effect on the soil bacterial community composition. According to the results of both methods, the soil bacterial community composition in agricultural lands (orchard and farm) was more similar than shrub land. Since HRM analysis is less expensive, easier and has less bias compared to DGGE technique, it is recommended to compare the composition of soil bacterial communities in different samples.
Keywords
Genomic DNA from soil; Soil bacterial 16S rDNA; Real-time PCR; Non-metric multidimensional scaling (NMDS)
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