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Ridha Abbas Al-Fahham, H, Raoof Kareem, K. (1401). Molecular Study of Urease ureR Gene of Proteus mirabilis Isolated from Urinary Tract Infections, Najaf, Iraq. سامانه مدیریت نشریات علمی, 77(3), 1257-1260. doi: 10.22092/ari.2022.357465.2042
H Ridha Abbas Al-Fahham; K Raoof Kareem. "Molecular Study of Urease ureR Gene of Proteus mirabilis Isolated from Urinary Tract Infections, Najaf, Iraq". سامانه مدیریت نشریات علمی, 77, 3, 1401, 1257-1260. doi: 10.22092/ari.2022.357465.2042
Ridha Abbas Al-Fahham, H, Raoof Kareem, K. (1401). 'Molecular Study of Urease ureR Gene of Proteus mirabilis Isolated from Urinary Tract Infections, Najaf, Iraq', سامانه مدیریت نشریات علمی, 77(3), pp. 1257-1260. doi: 10.22092/ari.2022.357465.2042
Ridha Abbas Al-Fahham, H, Raoof Kareem, K. Molecular Study of Urease ureR Gene of Proteus mirabilis Isolated from Urinary Tract Infections, Najaf, Iraq. سامانه مدیریت نشریات علمی, 1401; 77(3): 1257-1260. doi: 10.22092/ari.2022.357465.2042

Molecular Study of Urease ureR Gene of Proteus mirabilis Isolated from Urinary Tract Infections, Najaf, Iraq

Archives of Razi Institute
مقاله 43، دوره 77، شماره 3، مرداد و شهریور 2022، صفحه 1257-1260    اصل مقاله (400.75 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.357465.2042
نویسندگان
H Ridha Abbas Al-Fahham* ؛ K Raoof Kareem
Department of Medical Microbiology, Faculty of Pharmacy, Jabir Ibn Hayyan Medical University, Najaf, Iraq
چکیده
Proteus mirabilis is considered one of the causative pathogens that leads to complicated urinary tract infection (UTI); moreover, it produces urease. Urease plays a key role as a virulence factor for P. mirabilis. UreR, a member of the AraC/XylS family of transcriptional regulators, positively activates the expression of the ure gene cluster in the presence of urea. Therefore, this study was designed to investigate the contribution of ureR to urease activity and virulence in urinary tract infections. A total of 74 clinical samples were collected from August to December 2020. The urine samples were taken from individuals with parasitic infections in their urinary tracts. After cultivating the samples on the MacConkey agar, the initial identification was performed based on traditional methods with the automated VITEK-2 compact method. Bacterial isolates were inoculated by stabbing and streaking into a slant of urease agar, which were then incubated at 37°C for 24-48 h. The polymerase chain reaction technique was used to detect the P. mirabilis ureR gene. The results of biochemical studies were utilized to confirm the identification of P. mirabilis isolates that had previously been made. All isolates had the same oxidase-negative, catalase-positive, oxidase-negative, and catalase-positive properties. They were motile, methyl red, and uric acid, catalase, citrate, and urease positive. The results of investigating the expression of the ureR gene in 15 isolates of P. mirabilis suggested that only 14 (93.3%) of the isolates produced ureR gene products using unique primers.
کلیدواژه‌ها
Proteus mirabilis؛ ureR gene؛ PCR
مراجع
  1. Abbott SL. Klebsiella, enterobacter, citrobacter, serratia, plesiomonas, and Other Enterobacteriaceae. Manual of Clinical Microbiology, 10th Edition: American Society of Microbiology; 2011. p. 639-57.
  2. Al-Duliami AA, Nauman NG, Hasan A-RS, Al-Azawi ZH. Virulence factors of Proteus Mirabilis isolated from patients otitis media in Baquba and it’s peripheries. Diyala J Med. 2011;1(1):69-75.
  3. Ali HH, Yousif MG. Detection of some virulence factors genes of Proteus mirablis that isolated from urinary tract infection. Int J Adv Res. 2015;3(1):156-63.
  4. Bergsten G, Wullt B, Svanborg C. Escherichia coli, fimbriae, bacterial persistence and host response induction in the human urinary tract. Int J Med Microbiol. 2005;295(6):487-502.
  5. Burall LS, Harro JM, Li X, Lockatell CV, Himpsl SD, Hebel JR, et al. Proteus mirabilis genes that contribute to pathogenesis of urinary tract infection: identification of 25 signature-tagged mutants attenuated at least 100-fold. Infect Immun. 2004;72(5):2922-38.
  6. Mobley H, Island MD, Hausinger RP. Molecular biology of microbial ureases. Microbiol Rev. 1995;59(3):451-80.
  7. Dattelbaum JD, Lockatell CV, Johnson DE, Mobley HLT. UreR, the transcriptional activator of the Proteus mirabilis urease gene cluster, is required for urease activity and virulence in experimental urinary tract infections. Infect Immun. 2003;71(2):1026-30.
  8. Heimer SR, Mobley HL. Interaction of Proteus mirabilis urease apoenzyme and accessory proteins identified with yeast two-hybrid technology. J Bacteriol. 2001;183(4):1423-33.
  9. Al-Muhanna SG, Banoon SR, Al-Kraety IAA. Molecular Detection Of Integron Class 1 Gene In Proteus Mirabilis Isolated From Diabetic Foot Infections. Plant Arch. 2020;20(1):3101-7.
  10. Al-Kraety IAA, Al-Muhanna, SG, Banoon, SR. Molecular Exploring Of Plasmid-Mediated Ampc Beta Lactamase Gene In Clinical Isolates Of Proteus Mirabilis. Rev Bionatura J. 2021;6(3).
  1. D'Orazio SE, Thomas V, Collins CM. Activation of transcription at divergent urea‐dependent promoters by the urease gene regulator UreR. Mol Microbiol. 1996;21(3):643-55.
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