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Sami Awayid, H, Qassim Mohammad, S. (1401). Prevalence and Antibiotic Resistance Pattern of Methicillin-Resistant Staphylococcus aureus Isolated from Iraqi Hospitals. سامانه مدیریت نشریات علمی, 77(3), 1147-1156. doi: 10.22092/ari.2022.357391.2031
H Sami Awayid; S Qassim Mohammad. "Prevalence and Antibiotic Resistance Pattern of Methicillin-Resistant Staphylococcus aureus Isolated from Iraqi Hospitals". سامانه مدیریت نشریات علمی, 77, 3, 1401, 1147-1156. doi: 10.22092/ari.2022.357391.2031
Sami Awayid, H, Qassim Mohammad, S. (1401). 'Prevalence and Antibiotic Resistance Pattern of Methicillin-Resistant Staphylococcus aureus Isolated from Iraqi Hospitals', سامانه مدیریت نشریات علمی, 77(3), pp. 1147-1156. doi: 10.22092/ari.2022.357391.2031
Sami Awayid, H, Qassim Mohammad, S. Prevalence and Antibiotic Resistance Pattern of Methicillin-Resistant Staphylococcus aureus Isolated from Iraqi Hospitals. سامانه مدیریت نشریات علمی, 1401; 77(3): 1147-1156. doi: 10.22092/ari.2022.357391.2031

Prevalence and Antibiotic Resistance Pattern of Methicillin-Resistant Staphylococcus aureus Isolated from Iraqi Hospitals

Archives of Razi Institute
مقاله 28، دوره 77، شماره 3، مرداد و شهریور 2022، صفحه 1147-1156    اصل مقاله (563.64 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2022.357391.2031
نویسندگان
H Sami Awayid* 1؛ S Qassim Mohammad2
1Institute of Technical, Middle Technical University, Suwaira, Iraq
2Institute of Technical, Middle Technical University, Baqubah, Iraq
چکیده
Methicillin-resistant Staphylococcus aureus (MRSA) bacteria are often multi-drug resistant, resulting in a high rate of treatment failure. This study aimed to identify the antibiotics resistance profile and molecular characteristics of MRSA strains isolated from patients' samples, including skin, wounds, and burns, which are the most common infections, and collected from hospitals. The samples included 34 MRSA isolates gathered from January 2020 to September 2020. All isolates were tested using the Kirby-Bauer method to determine MRSA susceptibility against antibiotics using the minimum inhibitory concentration protocol and the E-test. The polymerase chain reaction was used for the detection of antibiotic resistance genes, including tetracycline, erythromycin, linezolid, gentamicin, rifampicin, ciprofloxacin, quinupristin-dalfopristin, clindamycin, and mecA. Staphylococcal Cassette Chromosome mec (SCCmec) was determined by multilocus sequence typing of all isolates; accordingly, the findings indicated that the sensitivity of linezolid, quinupristin-dalfopristin, rifampin, daptomycin, and vancomycin differed. Moreover, multidrug resistance of MRSA was shown to be more than 90% for penicillin and 91.1% for erythromycin. It was revealed that SCCmec III was resistant to at least four to five different antibiotics. ST585 (2.9%), ST240 (8.8%), ST45 (14.7%), ST22 (17.6%), and ST239 (higher rate) were the five sequence types found in STs (55.8%). Finally, it was indicated that the emergence of MRSA in these Iraqi hospitals highlighted further research to better understand how the infection might be effectively controlled.
کلیدواژه‌ها
MRSA؛ MLST؛ resistance gene؛ SCCmec
مراجع
  1. Grema HA, Geidam YA, Gadzama GB, Ameh JA, Suleiman A. sMethicillin resistant Staphylococcus aureus (MRSA): a review. Adv Anim Vet Sci. 2015;3(2):79-98.
  2. Monecke S, Coombs G, Shore AC, Coleman DC, Akpaka P, Borg M, et al. A field guide to pandemic, epidemic and sporadic clones of methicillin-resistant Staphylococcus aureus. PloS one. 2011;6(4):17936.
  3. Naves KSC, Trindade NVd, Gontijo Filho PP. Methicillin-resistant Staphylococcus aureus bloodstream infection: risk factors and clinical outcome in non-intensive-care units. Rev Soc Bras Med Trop. 2012;45:189-93.
  4. Jarvis WR, Jarvis AA, Chinn RY. National prevalence of methicillin-resistant Staphylococcus aureus in inpatients at United States health care facilities, 2010. Am J Infect Control. 2012;40(3):194-200.
  5. Graveland H, Duim B, Van Duijkeren E, Heederik D, Wagenaar JA. Livestock-associated methicillin-resistant Staphylococcus aureus in animals and humans. Int J Med Microbiol. 2011;301(8):630-4.
  6. Sydnor ER, Perl TM. Hospital epidemiology and infection control in acute-care settings. Clin Microbiol Rev. 2011;24(1):141-73.
  7. Negrini TdC, Duque C, de Oliveira ACM, Hebling J, Spolidorio L, Spolidorio D. Staphylococcus aureus contamination in a pediatric dental clinic. Int J Clin Pediatr Dent. 2009;34(1):13-8.
  8. Fasihi Y, Kiaei S, Kalantar-Neyestanaki D. Characterization of SCCmec and spa types of methicillin-resistant Staphylococcus aureus isolates from health-care and community-acquired infections in Kerman, Iran. J Epidemiol Glob Health. 2017;7(4):263-7.
  9. Shekarabi M, Hajikhani B, Salimi Chirani A, Fazeli M, Goudarzi M. Molecular characterization of vancomycin-resistant Staphylococcus aureus strains isolated from clinical samples: A three year study in Tehran, Iran. PloS one. 2017;12(8):0183607.
  10. Goudarzi M, Bahramian M, Tabrizi MS, Udo EE, Figueiredo AMS, Fazeli M, et al. Genetic diversity of methicillin resistant Staphylococcus aureus strains isolated from burn patients in Iran: ST239-SCCmec III/t037 emerges as the major clone. Microb Pathog. 2017;105:1-7.
  11. Kumar V, Ankola AV, Sankeshwari R, Jalihal S, Deepak V, Jois HS. Assessment of the antimicrobial efficacy of hydroalcoholic fruit extract of cranberry against Socransky complexes and predominant cariogenic, mycotic and endodontic climax communities of the oral cavity: An extensive in-vitro study. J Oral Maxillofac Pathol. 2019;23(3):407-411.
  12. Wayne P. Clinical and laboratory standards institute. Performance standards for antimicrobial susceptibility testing. 2011.
  13. Azimian A, Havaei SA, Fazeli H, Naderi M, Ghazvini K, Samiee SM, et al. Genetic characterization of a vancomycin-resistant Staphylococcus aureus isolate from the respiratory tract of a patient in a university hospital in northeastern Iran. J clin M 2012;50(11):3581-5.
  14. Lin J, Zhang T, Bai C, Liang J, Ye J, Yao Z. School environmental contamination of methicillin-sensitive Staphylococcus aureus as an independent risk factor for nasal colonization in schoolchildren: An observational, cross-sectional study. PloS one. 2018;13(11):0208183.
  15. Kouhsari E, Hosseini M, Ahmadi A, Khadivar P, Rahimi S, Amini A. Molecular Characterizations, Virulence Determinants and Antimicrobial Resistance Profiles of Methicillin-Resistant Staphylococcus aureus (MRSA) in the North of Iran. 2020.
  16. Boye K, Bartels M, Andersen I, Møller J, Westh H. A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I–V. Clin Microbiol and Infect. 2007;13(7):725-7.
  17. Enright MC, Day NP, Davies CE, Peacock SJ, Spratt BG. Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus. J clin 2000;38(3):1008-15.
  18. Schwaber MJ, Wright SB, Carmeli Y, Venkataraman L, DeGirolami PC, Gramatikova A, et al. Clinical Significance of Varying Degrees of Vancomycin Susceptilibity in Methicillin-Resistant Staphylococcus aureus Bacteremia. Emerg Infect Dis. 2003;9(6):657.
  1. Sachdev D, Amladi S, Nataraj G, Baveja S, Kharkar V, Mahajan S, et al. An outbreak of methicillin-resistant Staphylococcus aureus (MRSA) infection in dermatology indoor patients. Indian J Dermatol Venereol Leprol. 2003;69(6).
  2. Becker K, Eiff C. Staphylococcus , Micrococcus , and other catalase-positive cocci. 12011. p. 308-30.
  3. Ajayi A, Bibi O, Oniha M, Atolagbe O, Anosike S, Onibokun E. Studies on Staphylococcus aureus isolated from pimples. Pak J Biol Sci. 2017;20(7):350-4.
  4. Micheel V, Hogan B, Köller T, Warnke P, Crusius S, Hinz R, et al. Screening agars for MRSA: evaluation of a stepwise diagnostic approach with two different selective agars for the screening for methicillin-resistant Staphylococcus aureus (MRSA). Mil Med Res. 2015;2(1):1-7.
  5. Hraishawi RM. Phenotypic detection and vancomycin MICs for methicillin resistance Staphylococcus aureus isolated from nebulizer. EurAsian J BioSci. 2020;14(1).
  6. Hedin Gr, Fang H. Evaluation of two new chromogenic media, CHROMagar MRSA and S. aureus ID, for identifying Staphylococcus aureus and screening methicillin-resistant S. aureus. J Clin M 2005;43(8):4242-4.
  7. Kali A, Stephen S, Umadevi S. Laboratory evaluation of phenotypic detection methods of methicillin-resistant Staphylococcus aureus. Biomed J. 2014;37(6):411-4.
  8. Anand K, Agrawal P, Kumar S, Kapila K. Comparison of cefoxitin disc diffusion test, oxacillin screen agar, and PCR for mecA gene for detection of MRSA. Indian J Med Microbiol. 2009;27(1):27-9.
  9. Adaleti R, Nakipoglu Y, Karahan ZC, Tasdemir C, Kaya F. Comparison of polymerase chain reaction and conventional methods in detecting methicillin-resistant Staphylococcus aureus. J Infect Dev Ctries. 2008;2(01):046-50.
  10. Yıldız Ö, Çoban AY, Şener AG, Coşkuner SA, Bayramoğlu G, Güdücüoğlu H, et al. Antimicrobial susceptibility and resistance mechanisms of methicillin resistant Staphylococcus aureus isolated from 12 Hospitals in Turkey. Ann Clin Microbiol Antimicrob. 2014;13(1):1-6.
  11. Duran N, Ozer B, Duran GG, Onlen Y, Demir C. Antibiotic resistance genes & susceptibility patterns in staphylococci. Indian J Med Res. 2012;135(3):389.
  12. Prunier A-L, Malbruny B, Tandé D, Picard B, Leclercq R. Clinical isolates of Staphylococcus aureus with ribosomal mutations conferring resistance to macrolides. Antimicrob Agents Chemother. 2002;46(9):3054-6.
  13. Achek R, Hotzel H, Cantekin Z, Nabi I, Hamdi TM, Neubauer H, et al. Emerging of antimicrobial resistance in staphylococci isolated from clinical and food samples in Algeria. BMC Res Notes. 2018;11(1):1-7.
  14. Dormanesh B, Siroosbakhat S, Darian EK, Afsharkhas L. Methicillin-resistant Staphylococcus aureus isolated from various types of hospital infections in pediatrics: Panton-Valentine leukocidin, staphylococcal chromosomal cassette mec SCCmec phenotypes and antibiotic resistance properties. Jundishapur J Microbiol. 2015;8(11).
  15. Campanile F, Bongiorno D, Borbone S, Stefani S. Hospital-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) in Italy. Ann Clin Microbiol Antimicrob. 2009;8(1):1-10.
  16. Mahmoudi S, Mamishi S, Mohammadi M, Banar M, Ashtiani MTH, Mahzari M, et al. Phenotypic and genotypic determinants of mupirocin resistance among Staphylococcus aureus isolates recovered from clinical samples of children: an Iranian hospital-based study. Infect Drug Resist. 2019;12:137.
  17. Jung M-Y, Chung J-Y, Lee H-Y, Park J, Lee D-Y, Yang J-M. Antibiotic susceptibility of Staphylococcus aureus in atopic dermatitis: current prevalence of methicillin-resistant Staphylococcus aureus in Korea and treatment strategies. Ann Dermatol. 2015; 27(4):398-403.
  1. Goudarzi M, Seyedjavadi SS, Nasiri MJ, Goudarzi H, Nia RS, Dabiri H. Molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from patients with bacteremia based on MLST, SCCmec, spa, and agr locus types analysis. Microb Pathog. 2017;104:328-35.
  2. Mahdiyoun SM, Kazemian H, Ahanjan M, Houri H, Goudarzi M. Frequency of aminoglycoside-resistance genes in methicillin-resistant Staphylococcus aureus (MRSA) isolates from hospitalized patients. Jundishapur J Microbiol. 2016;9(8).
  3. Shahsavan S, Jabalameli L, Maleknejad P, Aligholi M, Imaneini H, Jabalameli F, et al. Molecular analysis and antimicrobial susceptibility of methicillin resistant Staphylococcus aureus in one of the hospitals of Tehran University of Medical Sciences: high prevalence of sequence type 239 (ST239) clone. Acta Microbiol Immunol Hung. 2011;58(1):31-9.
  4. Wang W-Y, Chiueh T-S, Sun J-R, Tsao S-M, Lu J-J. Molecular typing and phenotype characterization of methicillin-resistant Staphylococcus aureus isolates from blood in Taiwan. PloS One. 2012;7(1): 30394.
  5. Harris SR, Feil EJ, Holden MT, Quail MA, Nickerson EK, Chantratita N, et al. Evolution of MRSA during hospital transmission and intercontinental spread. Science. 2010;327(5964):469-74.
  6. Ohadian Moghadam S, Pourmand MR, Mahmoudi M, Sadighian H. Molecular characterization of methicillin-resistant Staphylococcus aureus: characterization of major clones and emergence of epidemic clones of sequence type (ST) 36 and ST 121 in Tehran, Iran. FEMS Microbiol Lett. 2015;362(8).
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