| Foot-and - mouth disease (FMD) is a highly contagious viral disease affecting cloven-hoofed animals. The particular virus that causes FMD disease is called FMD virus that is a member of the Aphthovirus genus in the Picornaviridae family. FMD virus has a single strain positive RNA genome with a length of 8500 nt with one ORF which are trapped in an icosahedral capsid protein. This virus genome doesn't have proofreading property which tends to highly mutagenesis. It has seven serotypes, such as, O, A, ASIA, SAT1, SAT2 and C and a lot of subtypes. Iran is an endemic region of foot-and - mouth disease. Vaccination of susceptible animals with inactivated whole-virus vaccine is the only way to control the epidemic in many developing countries .Today, conventionally attenuated and killed virus vaccines are being used worldwide. In Iran, animals have been vaccinated every 105 days with an inactivated FMD vaccine. Although commercially available FMD vaccines are effective, they provide short-term immunity requiring regular boosters. A new FMD vaccine is needed to improve immunization, safety and long-term immune responses. A synthetic peptide vaccine is one of the safe and important vaccines. Peptide vaccine has low immunogenicity and applying strong adjuvant is necessary. nanoliposomes as new adjuvants could be used to improve the immune response. In the current study, Nano liposomal carrier as an adjuvant containing two immunodominant synthetic FMDV peptides was selected. The efficacy of the vaccines was evaluated in guinea pigs. Indirect ELISA was used to detect FMDV-specific IgG in the serum of vaccinated guinea pigs. |
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