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Rashid Mahmood, A, Mansour Hussein, N. (1400). Study of Antibiotic Resistant Genes in Pseudomonas aeroginosa Isolated from Burns and Wounds. سامانه مدیریت نشریات علمی, 77(1), 403-411. doi: 10.22092/ari.2021.356681.1893
A Rashid Mahmood; N Mansour Hussein. "Study of Antibiotic Resistant Genes in Pseudomonas aeroginosa Isolated from Burns and Wounds". سامانه مدیریت نشریات علمی, 77, 1, 1400, 403-411. doi: 10.22092/ari.2021.356681.1893
Rashid Mahmood, A, Mansour Hussein, N. (1400). 'Study of Antibiotic Resistant Genes in Pseudomonas aeroginosa Isolated from Burns and Wounds', سامانه مدیریت نشریات علمی, 77(1), pp. 403-411. doi: 10.22092/ari.2021.356681.1893
Rashid Mahmood, A, Mansour Hussein, N. Study of Antibiotic Resistant Genes in Pseudomonas aeroginosa Isolated from Burns and Wounds. سامانه مدیریت نشریات علمی, 1400; 77(1): 403-411. doi: 10.22092/ari.2021.356681.1893

Study of Antibiotic Resistant Genes in Pseudomonas aeroginosa Isolated from Burns and Wounds

Archives of Razi Institute
مقاله 50، دوره 77، شماره 1، فروردین و اردیبهشت 2022، صفحه 403-411    اصل مقاله (621.29 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.356681.1893
نویسندگان
A Rashid Mahmood* ؛ N Mansour Hussein
Microbiology Department, School of Medicine, University of Kirkuk, Kirkuk, Iraq
چکیده
Pseudomonas aeruginosa (P. aeruginosa) is frequently associated with infections with high mortality rates. The intrinsically high resistance to many antibiotics and multidrug resistance in the hospital setting is considered to be among the reasons for high pathogenicity of P. aeruginosa. In this study, a total of 200 wound and burn swabs were collected from patients. The collected specimens were examined for P. aeruginosa through biochemical and antibacterial sensitivity tests performed in the Microbiology Laboratory in College of Medicine, University of Kirkuk, Kirkuk, Iraq. The polymerase chain reaction was then used to detect mexA, mexB, mexR, and oprD genes. In total, 31 isolates of P. aeruginosa were collected from 200 patients with wounds and burns. Most cases were isolated from 23 (74.19%) and 8 (25.80%) wound and burn swabs, respectively. Antibiotic sensitivity was tested on all isolates against 17 antimicrobial agents. The obtained results revealed a high resistance rate to gentamicin, trimethoprim, amikacin, and amoxicillin, and a low resistance rate was observed to ceftazidime, tobramycin, levofloxacin, cotrimoxazole, ciprofloxacin, and aztreonam. Regarding antibiotic resistance, mexB, mexR, and oprD genes were observed in three isolates, in which mexB and mexR were detected in two isolates, and only one isolate carried mexA gene.
کلیدواژه‌ها
Molecular diagnosis؛ Multidrug resistance؛ Pseudomonas aeruginosa
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مراجع
  1. Rhomberg PR, Fritsche TR, Sader HS, Jones RN. Antimicrobial susceptibility pattern comparisons among intensive care unit and general ward Gram-negative isolates from the Meropenem Yearly Susceptibility Test Information Collection Program (USA). Diagn Microbiol Infect Dis. 2006;56(1):57-62.
  2. Pryor KO, Fahey III TJ, Lien CA, Goldstein PA. Surgical site infection and the routine use of perioperative hyperoxia in a general surgical population: a randomized controlled trial. Jama. 2004;291(1):79-87.
  3. Roy R, Kataria V, Dhand M, Mahawal B, Samwal P. A surveillance study of bacterial flora associated with mobile phones in a tertiary care hospital. Int J Biomed Adv Res. 2013;4(1):56-8.
  4. America IDSo. The 10×'20 Initiative: pursuing a global commitment to develop 10 new antibacterial drugs by 2020. Clin Infect Dis. 2010;50(8):1081-3.
  5. Rice LB. Progress and challenges in implementing the research on ESKAPE pathogens. Infect Control Hosp Epidemiol. 2010;31(S1):7-10.
  6. Fernández L, Gooderham WJ, Bains M, McPhee JB, Wiegand I, Hancock RE. Adaptive resistance to the “last hope” antibiotics polymyxin B and colistin in Pseudomonas aeruginosa is mediated by the novel two-component regulatory system ParR-ParS. Antimicrob Agents Chemother. 2010;54(8):3372-82.
  7. Lescat M, Poirel L, Tinguely C, Nordmann P. A resazurin reduction-based assay for rapid detection of polymyxin resistance in Acinetobacter baumannii and Pseudomonas aeruginosa. J Clin Microbiol. 2019;57(3):e01563-18.
  8. Bălăşoiu M, Bălăşoiu A, Mănescu R, Avramescu C, Ionete O. Pseudomonas aeruginosa resistance phenotypes and phenotypic highlighting methods. Curr Health Sci J. 2014;40(2):85.
  9. Zeng Z-R, Wang W-P, Huang M, Shi L-N, Wang Y, Shao H-F. Mechanisms of carbapenem resistance in cephalosporin-susceptible Pseudomonas aeruginosa in China. Diagn Microbiol Infect Dis. 2014;78(3):268-70.
  10. Chaudhary M, Payasi A. Rising antimicrobial resistance of Pseudomonas aeruginosa isolated from clinical specimens in India. J Proteomics Bioinform. 2013;6(1):005-9.
  11. Lambert P. Mechanisms of antibiotic resistance in Pseudomonas aeruginosa. J R Soc Med. 2002;95(41):22.
  12. Winn Washington C, Allen SD, Janda WM, Koneman EW, Procop GW, Schreckenberger PC, et al. Koneman's Color Atlas and Textbook of Diagnostic Microbiolgy: Lippincott, Williams & Wilkins; 2006.
  13. Banerjee S, Batabyal K, Joardar S, Isore D, Dey S, Samanta I, et al. Detection and characterization of pathogenic Pseudomonas aeruginosa from bovine subclinical mastitis in West Bengal, India. Vet World. 2017;10(7):738.
  14. Hudzicki J. Kirby-Bauer disk diffusion susceptibility test protocol. 2009.
  15. Wayne P. Clinical and laboratory standards institute. Performance standards for antimicrobial susceptibility testing. 2011.
  16. Strateva T, Ouzounova-Raykova V, Markova B, Todorova A, Marteva-Proevska Y, Mitov I. Problematic clinical isolates of Pseudomonas aeruginosa from the university hospitals in Sofia, Bulgaria: current status of antimicrobial resistance and prevailing resistance mechanisms. J Med Microbiol. 2007;56(7):956-63.
  17. Ullah W, Qasim M, Rahman H, Khan S, ur Rehman Z, Ali N, et al. CTX-M-15 and OXA-10 beta lactamases in multi drug resistant Pseudomonas aeruginosa: first report from Pakistan. Microb Pathog. 2017;105:240-4.
  18. Kanj SS, Kanafani ZA, editors. Current concepts in antimicrobial therapy against resistant gram-negative organisms: extended-spectrum β-lactamase–producing enterobacteriaceae, carbapenem-resistant enterobacteriaceae, and multidrug-resistant Pseudomonas aeruginosa. Mayo Clinic Proceedings; 2011: Elsevier.
  19. Shahzad MN, Ahmed N, H KI, B MA, F W. Bacterial profile of burn wound infections in burn patients. Ann Pak Inst Med Sci. 2012;8(1):54-7.
  20. Feretzakis G, Loupelis E, Sakagianni A, Skarmoutsou N, Michelidou S, Velentza A, et al. A 2-year single-centre audit on antibiotic resistance of Pseudomonas aeruginosa, Acinetobacter baumannii and Klebsiella pneumoniae strains from an intensive care unit and other wards in a general public hospital in Greece. Antibiotics. 2019;8(2):62.
  21. Sambrano H, Castillo JC, Ramos CW, de Mayorga B, Chen O, Durán O, et al. Prevalence of antibiotic resistance and virulent factors in nosocomial clinical isolates of Pseudomonas aeruginosa from Panamá. Braz J Infect Dis. 2021;25.
  22. Yokoyama K, Doi Y, Yamane K, Kurokawa H, Shibata N, Shibayama K, et al. Acquisition of 16S rRNA methylase gene in Pseudomonas aeruginosa. lancet. 2003;362(9399):1888-93.
  23. Rosanova MT, Stamboulian D, Lede R. Risk factors for mortality in burn children. Braz J Infect Dis. 2014;18:144-9.
  24. Ugwuanyi FC, Ajayi A, Ojo DA, Adeleye AI, Smith SI. Evaluation of efflux pump activity and biofilm formation in multidrug resistant clinical isolates of Pseudomonas aeruginosa isolated from a Federal Medical Center in Nigeria. Ann Clin Microbiol Antimicrob. 2021;20(1):1-7.
  25. Agnihotri N, Gupta V, Joshi R. Aerobic bacterial isolates from burn wound infections and their antibiograms—a five-year study. Burns. 2004;30(3):241-3.
  26. Nouér SA, Nucci M, de-Oliveira MP, Pellegrino FLPC, Moreira BM. Risk factors for acquisition of multidrug-resistant Pseudomonas aeruginosa producing SPM metallo-β-lactamase. Antimicrob Agents Chemother. 2005;49(9):3663-7.
  27. Ekrami A, Kalantar E. Bacterial infections in burn patients at a burn hospital in Iran. Indian J Med Res. 2007;126(6):541.
  28. Bukharie H, Mowafi H. Antimicrobial susceptibility pattern of Pseudomonas aeruginosa and antibiotic use in King Fahad Hospital of the University in Khobar, Saudi Arabia. Sci J King Faisal Univ. 2010;11(1):183-90.
  29. Aminizadeh Z, Kashi MS. Prevalence of multi-drug resistance and pandrug resistance among multiple gram-negative species: experience in one teaching hospital, Tehran, Iran. Int Res J Microbiol. 2011;2(3):90-5.
  30. Aghazadeh M, Hojabri Z, Mahdian R, Nahaei MR, Rahmati M, Hojabri T, et al. Role of efflux pumps: MexAB-OprM and MexXY (-OprA), AmpC cephalosporinase and OprD porin in non-metallo-β-lactamase producing Pseudomonas aeruginosa isolated from cystic fibrosis and burn patients. Infect Genet Evol. 2014;24:187-92.
  31. Livermore DM. Multiple mechanisms of antimicrobial resistance in Pseudomonas aeruginosa: our worst nightmare? Clin Infect Dis. 2002;34(5):634-40.
  32. Pasca MR, Dalla Valle C, De Jesus Lopes Ribeiro AL, Buroni S, Papaleo MC, Bazzini S, et al. Evaluation of fluoroquinolone resistance mechanisms in Pseudomonas aeruginosa multidrug resistance clinical isolates. Microb Drug Resist. 2012;18(1):23-32.
  33. Kishk RM, Abdalla MO, Hashish AA, Nemr NA, El Nahhas N, Alkahtani S, et al. Efflux MexAB-mediated resistance in P. aeruginosa isolated from patients with healthcare associated infections. Pathogens. 2020;9(6):471.
  34. Ferrer-Espada R, Shahrour H, Pitts B, Stewart PS, Sánchez-Gómez S, Martínez-de-Tejada G. A permeability-increasing drug synergizes with bacterial efflux pump inhibitors and restores susceptibility to antibiotics in multi-drug resistant Pseudomonas aeruginosa strains. Sci Rep. 2019;9(1):1-12.
  35. Tian Z-X, Yi X-X, Cho A, O’Gara F, Wang Y-P. CpxR activates MexAB-OprM efflux pump expression and enhances antibiotic resistance in both laboratory and clinical nalB-type isolates of Pseudomonas aeruginosa. PLoS pathog. 2016;12(10):e1005932.
  1. Siriyong T, Srimanote P, Chusri S, Yingyongnarongkul B-e, Suaisom C, Tipmanee V, et al. Conessine as a novel inhibitor of multidrug efflux pump systems in Pseudomonas aeruginosa. BMC Complement Altern Med. 2017;17(1):1-7.
  2. Fernández L, Hancock RE. Adaptive and mutational resistance: role of porins and efflux pumps in drug resistance. Clin Microbiol Rev. 2012;25(4):661-81.
  3. Tamber S, Maier E, Benz R, Hancock RE. Characterization of OpdH, a Pseudomonas aeruginosa porin involved in the uptake of tricarboxylates. J Bacteriol. 2007;189(3):929-39.
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