اخبار و اعلانات

 آمار

تعداد نشریات286
تعداد نشریات سازمان84
تعداد شماره‌ها3,029
تعداد مقالات33,889
تعداد مشاهده مقاله45,265,085
تعداد دریافت فایل اصل مقاله61,018,573
Zaki Mahdi, N. (1400). Investigation of the Lethal Effect of Purified Arginine Deiminase Purified from Lactobacillus plantarum p5 on Murine Mammary Adenocarcinoma and Vero cell Lines. سامانه مدیریت نشریات علمی, 77(1), 241-247. doi: 10.22092/ari.2021.356587.1873
N Zaki Mahdi. "Investigation of the Lethal Effect of Purified Arginine Deiminase Purified from Lactobacillus plantarum p5 on Murine Mammary Adenocarcinoma and Vero cell Lines". سامانه مدیریت نشریات علمی, 77, 1, 1400, 241-247. doi: 10.22092/ari.2021.356587.1873
Zaki Mahdi, N. (1400). 'Investigation of the Lethal Effect of Purified Arginine Deiminase Purified from Lactobacillus plantarum p5 on Murine Mammary Adenocarcinoma and Vero cell Lines', سامانه مدیریت نشریات علمی, 77(1), pp. 241-247. doi: 10.22092/ari.2021.356587.1873
Zaki Mahdi, N. Investigation of the Lethal Effect of Purified Arginine Deiminase Purified from Lactobacillus plantarum p5 on Murine Mammary Adenocarcinoma and Vero cell Lines. سامانه مدیریت نشریات علمی, 1400; 77(1): 241-247. doi: 10.22092/ari.2021.356587.1873

Investigation of the Lethal Effect of Purified Arginine Deiminase Purified from Lactobacillus plantarum p5 on Murine Mammary Adenocarcinoma and Vero cell Lines

Archives of Razi Institute
مقاله 33، دوره 77، شماره 1، فروردین و اردیبهشت 2022، صفحه 241-247    اصل مقاله (619.29 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.356587.1873
نویسنده
N Zaki Mahdi*
Department of Biology, College of Sciences, Mustansiriyah University, Baghdad, Iraq
چکیده
Cancer is one of the most serious diseases facing humanity, so we urgently need to find a cure which is rarely harmful to the patient as much as possible. It has been approved that arginine deiminase (ADI) can hydrolyze the plasma arginine to citrulline. This hydrolysis activity and reduction in the amount of inter cellular argenine suppress lipopolysaccharide-induced nitric oxide (NO) synthesis. On the other hand arginine depletion, arresting the cell cycle at G1 phase, Therefore, ADI has considered as a powerful anticancer agents. The aim of the current study was to investigate the lethal effects of ADI purified from Lactobacillus plantarum p5 strain on murine mammary adenocarcinoma and vero cell lines. Anti-proliferative activity of ADI against murine mammary adenocarcinoma )AMN3) cell line was evaluated after different incubation time (3, 6, 24, 48 and 72 h) of exposure to 1 µg/mL of ADI compared with Vero (none cancer cell line ) transformed cell line with same conditions. Autophagy process in cancer cells was recognized after three hours of incubation with ADI which was clearly observed in AMN3 cell line under inverted microscope. First stages of programmed cell death (apoptosis) pathway was only observed in AMN3 cells after 24 h of incubation with ADI and this process continued with the time until they reached to last stages of apoptosis after 72 h of incubation. The results of the current study showed that the AMN3 cell line was auxotrophic for arginine because it couldn't produce it in the presence of enzyme which had a robust activity to kill these cancer cells, but Vero none cancer cell line was survive in the presence of ADI because it had the ability to produce arginine.
کلیدواژه‌ها
AMN3؛ Arginine Deiminase؛ Autophagy؛ Lactobacillus plantarum p5؛ Vero
سایر فایل های مرتبط با مقاله
مراجع
  1. Mehrpour M, Esclatine A, Beau I, Codogno P. Autophagy in health and disease. 1. Regulation and significance of autophagy: an overview. Am J Physiol Cell Physiol. 2010;298(4):C776-85.
  2. Karanasios E, Stapleton E, Walker SA, Manifava M, Ktistakis NT. Live cell imaging of early autophagy events: omegasomes and beyond. J Vis Exp. 2013(77).
  3. Klionsky DJ. Autophagy revisited: a conversation with Christian de Duve. Autophagy. 2008;4(6):740-3.
  4. Vrancken G, Rimaux T, Wouters D, Leroy F, De Vuyst L. The arginine deiminase pathway of Lactobacillus fermentum IMDO 130101 responds to growth under stress conditions of both temperature and salt. Food Microbiol. 2009;26(7):720-7.
  5. Qiu F, Huang J, Sui M. Targeting arginine metabolism pathway to treat arginine-dependent cancers. Cancer Lett. 2015;364(1):1-7.
  6. Tsai HJ, Jiang SS, Hung WC, Borthakur G, Lin SF, Pemmaraju N, et al. A Phase II Study of Arginine Deiminase (ADI-PEG20) in Relapsed/Refractory or Poor-Risk Acute Myeloid Leukemia Patients. Sci Rep. 2017;7(1):11253.
  7. Mahdi N, Al-Tahan S, Yaseen N. Cytotoxicity and Apoptosis Effect of Purified Arginine Deiminase(ADI) Originating From Enterococcus Faecium M1 on Rhabdomyosarcoma(RD) Cancer Cell Line and Rat Embryo Fibroblast (REF) normal cell line. Iraqi J Microbiol. 2015;14(2):12-29.
  8. Mahdi N, Al-Tahan S, Yaseen N. Cytotoxicity and apoptosis of glioblastoma multiforme cell line by arginine deiminase purified from a higher productive isolation Enterococcus faecium M1. World J Pharm Sci. 2016;4(8):98-106.
  9. Mah LY, Ryan KM. Autophagy and cancer. Cold Spring Harb Perspect Biol. 2012;4(1):a008821.
  10. Yee KS, Wilkinson S, James J, Ryan KM, Vousden KH. PUMA- and Bax-induced autophagy contributes to apoptosis. Cell Death Differ. 2009;16(8):1135-45.
  1. Park IS, Kang SW, Shin YJ, Chae KY, Park MO, Kim MY, et al. Arginine deiminase: a potential inhibitor of angiogenesis and tumour growth. Br J Cancer. 2003;89(5):907-14.
  2. Xiong L, Teng JL, Botelho MG, Lo RC, Lau SK, Woo PC. Arginine Metabolism in Bacterial Pathogenesis and Cancer Therapy. Int J Mol Sci. 2016;17(3):363.
  3. Dhyea R, Abdul jalill R. Antagonistic effect between Citrullus colocynthis ex-tract and TiO2 nanoparticles in anticancer combina-tion therapy. 2016;9:195-204.
  4. Kahraman H, Karaderi C. The importance of arginine deiminase. Arch Chem and Chem Engin. 2019;1(1):854-70.
  5. Freshney R. New York: John Wiley and Sons Inc. 2005.
  6. Riess C, Shokraie F, Classen CF, Kreikemeyer B, Fiedler T, Junghanss C, et al. Arginine-Depleting Enzymes - An Increasingly Recognized Treatment Strategy for Therapy-Refractory Malignancies. Cell Physiol Biochem. 2018;51(2):854-70.
  7. Rybstein MD, Bravo-San Pedro JM, Kroemer G, Galluzzi L. The autophagic network and cancer. Nat Cell Biol. 2018;20(3):243-51.
  8. Zam W. Arginine enzymatic deprivation and diet restriction for cancer treatment. Braz J Pharm Sci. 2017;53.
آمار
تعداد مشاهده مقاله: 1,306
تعداد دریافت فایل اصل مقاله: 1,926


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب