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Dekami, Abbas, Sanjarian, Forough, Chaichi, Mehrdad, Hosseini, Bahman, Rahnama, Hassan. (1402). Effect of Plant Growth Regulators and Light on Callus Induction, Antioxidant Enzyme Response and Total Phenol in Nigella arvensis L.. سامانه مدیریت نشریات علمی, 12(1), 47-56. doi: 10.22092/jmpb.2021.354951.1369
Abbas Dekami; Forough Sanjarian; Mehrdad Chaichi; Bahman Hosseini; Hassan Rahnama. "Effect of Plant Growth Regulators and Light on Callus Induction, Antioxidant Enzyme Response and Total Phenol in Nigella arvensis L.". سامانه مدیریت نشریات علمی, 12, 1, 1402, 47-56. doi: 10.22092/jmpb.2021.354951.1369
Dekami, Abbas, Sanjarian, Forough, Chaichi, Mehrdad, Hosseini, Bahman, Rahnama, Hassan. (1402). 'Effect of Plant Growth Regulators and Light on Callus Induction, Antioxidant Enzyme Response and Total Phenol in Nigella arvensis L.', سامانه مدیریت نشریات علمی, 12(1), pp. 47-56. doi: 10.22092/jmpb.2021.354951.1369
Dekami, Abbas, Sanjarian, Forough, Chaichi, Mehrdad, Hosseini, Bahman, Rahnama, Hassan. Effect of Plant Growth Regulators and Light on Callus Induction, Antioxidant Enzyme Response and Total Phenol in Nigella arvensis L.. سامانه مدیریت نشریات علمی, 1402; 12(1): 47-56. doi: 10.22092/jmpb.2021.354951.1369

Effect of Plant Growth Regulators and Light on Callus Induction, Antioxidant Enzyme Response and Total Phenol in Nigella arvensis L.

Journal of Medicinal plants and By-products
دوره 12، شماره 1، خرداد 2023، صفحه 47-56    اصل مقاله (1.17 M)
نوع مقاله: Research Article
شناسه دیجیتال (DOI): 10.22092/jmpb.2021.354951.1369
نویسندگان
Abbas Dekami1، 2؛ Forough Sanjarian* 1؛ Mehrdad Chaichi3؛ Bahman Hosseini2؛ Hassan Rahnama4
1Plant Bio-Product group, Agricultural Biotechnology Institute, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran. Iran
2Department of Horticulture, Faculty of Agriculture, Urmia University, Urmia, Iran
3Department of Seed and Plant Improvement Research, Hamedan, Agricultural and Natural Resources, Research and Education Center, Agricultural Research, Education and Extension Organization, Hamedan, Iran
4Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
چکیده
The tissue culture of Nigella arvensis L. was carried out on an MS medium supplemented with various concentrations of auxins and cytokinins. Media fortified with different concentrations (0.0, 0.5, 1.0 and 1.5 mg/L) of 2, 4-D, NAA or IBA in combination with kinetin or BAP (1.5 mg/L and 2 mg/L) were used to induce and grow callus in present and absent of light. The experimental design was performed as a randomized block factorial design with three replications. Following callus culture, antioxidant enzymes activity and total phenolic contents were evaluated. The maximum callus induction (80.9%) was obtained with 1.0 mg/L 2, 4-D and 1.5 mg/L/ kinetin in the presence of light. Plant growth regulators combination and concentration, as well as photoperiod, affected the activity of antioxidant enzymes. The highest activities of catalase and peroxidase and total phenolic contents were measured in media with BAP combinations. On the contrary, the activity of superoxide dismutase was at the least in all BAP combinations. Overall, light intensified the activities of enzymes, but it had a negative effect on total phenolic contents.
کلیدواژه‌ها
Antioxidant Enzyme؛ Nigella Arvensis؛ Plant Growth Regulators؛ Plant cell Culture؛ Total Phenolic Content
مراجع
  1. Li J.W.H., Vederas J.C. Drug Discovery and Natural Products: End of an Era or an Endless Frontier? Sci. 2009; 325: 161-165.
  2. Mustafa G., Arif R., Atta A., Sharif S., Jamil A. Bioactive Compounds from Medicinal Plants and Their Importance in Drug Discovery in Pakistan. Matrix Science Pharma (MSP). 2017;1: 17-26.
  3. Sudhir S., Kumarappan A., Malakar J., Verma H. Genetic diversity of Nigella sativa from different geographies using RAPD markers. American Life Sci J. 2016;4: 175-180.
  4. Bardideh F., Kahrizi D., Ghobadi M.E. Interaction between the different irrigation regimes on grain weight and Nigella sativa seed essential oil percentage (Nigella sativa L.). Gazi Univertesi Gazi Egitim Fakultesi Tesi Dergisi. 2015;4: 1-11.
  5. Malhotra S.K. Nigella. In Peter, K.V. Handbook of Herbs and Spices (Second edition). Woodhead Publishing. 2012,pp. 391-416.
  6. Ahmad A., Husain A., Mujeeb M., Khan S.A., Najmi A.K., Siddique N.A., Damanhouri Z.A., Anwar F. A review on therapeutic potential of Nigella sativa: A miracle herb. Asian Pacific Tropical Biomedicine J. 2013;3: 337-352
  7. Alemi M., Sabouni F., Sanjarian F., Haghbeen K., Ansari S. Anti-inflammatory Effect of Seeds and Callus of Nigella sativa L. Extracts on Mix Glial Cells with Regard to Their Thymoquinone Content. AAPS Pharm Sci Tech. 2013;14: 160-167.
  8. Rashid M., Sanjarin F., Sabouni F. Thymoquinone effects on cell viability, apoptosis and VEGF-A gene expression level in AGS (CRL-1739) cell line. Anti-Cancer Agents in Medicinal Chem. 2019;19: 820-826.
  9. Ratz-Łyko A., Herman A., Arct J., Pytkowska K. Evaluation of antioxidant and antimicrobial activities of Oenothera biennis, Borago officinalis, and Nigella sativa seedcake extracts. Food Sci and Biotechnology. 2014;23: 1029-1036.
  10. Bıçak K, Gülcemal D, Demirtaş I, Alankuş Ö. Novel saponins from Nigella arvensis var. involucrata. Phytochemistry Letters. 2017; 21:128-133.
  11. Lin D., Xiao M., Zhao J., Li Z., Xing B., Li X., Kong M., Li L., Zhang Q., Liu Y. An Overview of Plant Phenolic Compounds and Their Importance in Human Nutrition and Management of Type 2 Diabetes. Molecules. 2016;21:1374.
  12. Martinez K.B., Mackert J.D., McIntosh M.K. Polyphenols and Intestinal Health A2 - Watson, Ronald Ross. Nutrition and Functional Foods for Healthy Aging. Academic Press. 2017. pp. 191-210.
  13. Gonçalves S., Romano A. Production of plant secondary metabolites by using biotechnological tools. Secondary Metabolites-Sources and Applications. 2018; 81-99.
  14. Ochoa-Villarreal M., Howat S., Hong S., Jang M.O., Jin Y.W., Lee E.K., Loake G.J. Plant cell culture strategies for the production of natural products. BMB Reports. 2016;49:149-158.
  15. Strydhorst S., Hall L., Perrott L. Plant growth regulators: What agronomists need to know. Crops and Soils. 2018;51: 22-26.
  16. Dou H., Niu G., Gu M., Masabni J. Effects of light quality on growth and phytonutrient accumulation of herbs under controlled environments. Horticulturae. 2017; 3:36.
  17. Janků M., Luhová L., Petřivalský M. On the Origin and Fate of Reactive Oxygen Species in Plant Cell Compartments. Antioxidants. 2019; 8: 105.
  18. Mittler R. ROS are good. Trends in Plant Science. 2017;22: 11-19.
  19. Murashige T., Skoog F. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum. 1962;15:473-497.
  20. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry. 1976;72:248-254.
  21. Gholamnezhad J., Sanjarian F., Goltapeh E., Safaie N., Razavi K. Effect of salicylic acid on enzyme activity in wheat in immediate early time after infection with Mycosphaerella graminicola. Scientia Agric Bohemica. 2016; 47: 1-8.
  22. Jahantigh O., Najafi F., Badi H.N., Khavari-Nejad R.A., Sanjarian F. Changes in antioxidant enzymes activities and proline, total phenol and anthocyanine contents in Hyssopus officinalis L. plants under salt stress. Acta Biologica Hungarica. 2016;67: 195-204.
  23. Zhang Y., Schmid Y.R., Luginbühl S., Wang Q., Dittrich P.S., Walde P. Spectrophotometric Quantification of Peroxidase with p-Phenylene-diamine for Analyzing Peroxidase-Encapsulating Lipid Vesicles. Analytical Chem. 2017;89: 5484-5493.
  24. Ainsworth E.A., Gillespie K.M. Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin–Ciocalteu reagent. Nature Protocols. 2007;2: 875-877.
  25. Yasmeen H., Hassnain S. Comparative analysis of different bioactivities of Curcuma longa, Nigella sativa seeds, and Camellia sinensis extracted by four different methods: A green way to reduce oxidative stress. Food Science and Biotechnology. 2016;25: 811-819.
  26. Mulabagal V., Tsay H.S. Plant cell cultures-an alternative and efficient source for the production of biologically important secondary metabolites. Int Appl Sci Eng J. 2004; 2: 29-48.
  27. Rao S.R., Ravishankar G. Plant cell cultures: chemical factories of secondary metabolites. Biotechnology Advances. 2002;20:101-153.
  28. Tripathi L., Tripathi J.N. Role of biotechnology in medicinal plants. Tropical Pharmaceu Res J. 2003;2: 243-253.
  29. Ikeuchi M., Sugimoto K., Iwase A. Plant callus: mechanisms of induction and repression. The Plant Cell. 2013;25: 3159-3173.
  30. Dhar U., Joshi M. Efficient plant regeneration protocol through callus for Saussurea obvallata (DC.) Edgew.(Asteraceae): effect of explant type, age and plant growth regulators. Plant Cell Reports. 2005;24: 195-200.
  31. Prakasha A., Umesha S. Effect of growth hormones in induction of callus, antioxidants, and antibacterial activity in Nerium odorum. Applied Biol & Biotech J. 2018;6: 21-25.
  32. Ibrahim M.M., Arafa N.M., Matter M.A. Effect of some elicitors on chemicals composition for Nigella sativa callus cultures. World Pharm Sci J. 2015;3: 2160-2166.
  33. Chaudhry H., Fatima N., Ahmad I. Establishment of callus and cell suspension cultures of Nigella sativa L. For thymol production. Inter Pharmacy and Pharmaceutical Sci J. 2014; 6;788-794.
  34. Jabeen N., Shawl A., Dar G., Sultan P. Callus induction and organogenesis from explants of Aconitum heterophyllum medicinal plant. Biotechnology. 2006;5: 287-291.
  35. Yu Y., Qin W., Li Y., Zhang C., Wang Y., Yang Z., Ge X., Li F. Red light promotes cotton embryogenic callus formation by influencing endogenous hormones, polyamines and antioxidative enzyme activities. Plant Growth Regulation. 2019;87:187-199.
  36. Shah M., Ullah M.A., Drouet S., Younas M., Tungmunnithum D., Giglioli-Guivarc’h N., Hano C., Abbasi B.H. Interactive Effects of Light and Melatonin on Biosynthesis of Silymarin and Anti-Inflammatory Potential in Callus Cultures of Silybum marianum (L.) Gaertn. Molecules. 2019;24: 1207.
  37. Adil M., Ren X., Jeong B.R. Light elicited growth, antioxidant enzymes activities and production of medicinal compounds in callus culture of Cnidium officinale Makino. Photochemistry and Photobiology B: Biology J. 2019;196: 111509.
  38. Ud Din N., Ali H., Shah S.K., Israr S.F., Ali M.S., Khan R.S., Khan M.A. Relationship of light intensity and quality with callus biomass and antioxidant potential in Ajuga bracteosa. Int Biosci J. 2019;15(1): 506-516.
  39. Martinez M.E., Jorquera L., Poirrier P., Díaz K., Chamy R. Effect of the Carbon Source and Plant Growth Regulators (PGRs) in the Induction and Maintenance of an In Vitro Callus Culture of Taraxacum officinale (L) Weber Ex FH Wigg. Agronomy. 2021;11:1181.
  40. Quamruzzaman M., Manik S.M.N., Shabala S., Zhou M. Improving Performance of Salt-Grown Crops by Exogenous Application of Plant Growth Regulators. Biomolecules. 2021;11: 788.
  41. Shah S. Kinetin improves photosynthetic and antioxidant responses of Nigella sativa to counteract salt stress. Russian Plant Physiology J. 2011;58: 454-459.
  42. Baque M.A., Hahn E.J., Paek K.Y. Growth, secondary metabolite production and antioxidant enzyme response of Morinda citrifolia adventitious root as affected by auxin and cytokinin. Plant Biotechnology Reports. 2010;4:109-116.
  43. Adil M., Ren X., Kang D.I., Jeong B.R. Effect of explant type and plant growth regulators on callus induction, growth and secondary metabolites production in Cnidium officinale Makino. Molecular Biology Reports. 2018; 1-9.
  44. Jain S.C., Pancholi B., Jain R. In-vitro callus propagation and secondary metabolite quantification in Sericostoma pauciflorum. Iranian Pharmaceutical Res J. 2012;11: 1103.
  45. Ali H., Khan M.A., Ullah N., Khan R.S. Impacts of hormonal elicitors and photoperiod regimes on elicitation of bioactive secondary volatiles in cell cultures of Ajuga bracteosa. J Photochemistry and Photobiology B: Biology. 2018;183: 242-250.
  46. Sigaud-Kutner T.C., Neto A.M., Pinto E., Colepicolo P. Diel activities of antioxidant enzymes, photosynthetic pigments and malondialdehyde content in stationary-phase cells of Tetraselmis gracilis (Prasinophyceae). Aquatic botany. 2005;82: 239-249.
  47. Coimbra M.C., Chagas R.C.R., Duarte-Almeida J.M., Castro A.H.F. Influence of plant growth regulators and light on callus induction and bioactive phenolic compounds production in Pyrostegia venusta (Bignoniaceae). Indian Exp Biol J. 2017; 55:584–590.
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