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Dekami, Abbas, Sanjarian, Forough, Chaichi, Mehrdad, Hosseini, Bahman, Rahnama, Hassan. (1402). Effect of Plant Growth Regulators and Light on Callus Induction, Antioxidant Enzyme Response and Total Phenol in Nigella arvensis L.. سامانه مدیریت نشریات علمی, 12(1), 47-56. doi: 10.22092/jmpb.2021.354951.1369
Abbas Dekami; Forough Sanjarian; Mehrdad Chaichi; Bahman Hosseini; Hassan Rahnama. "Effect of Plant Growth Regulators and Light on Callus Induction, Antioxidant Enzyme Response and Total Phenol in Nigella arvensis L.". سامانه مدیریت نشریات علمی, 12, 1, 1402, 47-56. doi: 10.22092/jmpb.2021.354951.1369
Dekami, Abbas, Sanjarian, Forough, Chaichi, Mehrdad, Hosseini, Bahman, Rahnama, Hassan. (1402). 'Effect of Plant Growth Regulators and Light on Callus Induction, Antioxidant Enzyme Response and Total Phenol in Nigella arvensis L.', سامانه مدیریت نشریات علمی, 12(1), pp. 47-56. doi: 10.22092/jmpb.2021.354951.1369
Dekami, Abbas, Sanjarian, Forough, Chaichi, Mehrdad, Hosseini, Bahman, Rahnama, Hassan. Effect of Plant Growth Regulators and Light on Callus Induction, Antioxidant Enzyme Response and Total Phenol in Nigella arvensis L.. سامانه مدیریت نشریات علمی, 1402; 12(1): 47-56. doi: 10.22092/jmpb.2021.354951.1369

Effect of Plant Growth Regulators and Light on Callus Induction, Antioxidant Enzyme Response and Total Phenol in Nigella arvensis L.

Journal of Medicinal plants and By-products
دوره 12، شماره 1، خرداد 2023، صفحه 47-56    اصل مقاله (1.17 M)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22092/jmpb.2021.354951.1369
نویسندگان
Abbas Dekami1، 2؛ Forough Sanjarian* 1؛ Mehrdad Chaichi3؛ Bahman Hosseini2؛ Hassan Rahnama4
1Plant Bio-Product group, Agricultural Biotechnology Institute, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran. Iran
2Department of Horticulture, Faculty of Agriculture, Urmia University, Urmia, Iran
3Department of Seed and Plant Improvement Research, Hamedan, Agricultural and Natural Resources, Research and Education Center, Agricultural Research, Education and Extension Organization, Hamedan, Iran
4Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran
چکیده
The tissue culture of Nigella arvensis L. was carried out on an MS medium supplemented with various concentrations of auxins and cytokinins. Media fortified with different concentrations (0.0, 0.5, 1.0 and 1.5 mg/L) of 2, 4-D, NAA or IBA in combination with kinetin or BAP (1.5 mg/L and 2 mg/L) were used to induce and grow callus in present and absent of light. The experimental design was performed as a randomized block factorial design with three replications. Following callus culture, antioxidant enzymes activity and total phenolic contents were evaluated. The maximum callus induction (80.9%) was obtained with 1.0 mg/L 2, 4-D and 1.5 mg/L/ kinetin in the presence of light. Plant growth regulators combination and concentration, as well as photoperiod, affected the activity of antioxidant enzymes. The highest activities of catalase and peroxidase and total phenolic contents were measured in media with BAP combinations. On the contrary, the activity of superoxide dismutase was at the least in all BAP combinations. Overall, light intensified the activities of enzymes, but it had a negative effect on total phenolic contents.
کلیدواژه‌ها
Antioxidant Enzyme؛ Nigella Arvensis؛ Plant Growth Regulators؛ Plant cell Culture؛ Total Phenolic Content
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