اخبار و اعلانات

 آمار

تعداد نشریات285
تعداد نشریات سازمان83
تعداد شماره‌ها3,086
تعداد مقالات34,442
تعداد مشاهده مقاله48,913,380
تعداد دریافت فایل اصل مقاله79,352,352
Pavlova, T. V, Pilkevich, N. B, Bessmertnyi, D. V, Pavlov, I. A, Atiakshin, D. V, Pavlova, L. A. (1400). Evaluation of New Immunohistochemical Approaches for the Study of Kidney Tumors in Geriatric. سامانه مدیریت نشریات علمی, 76(4), 1107-1113. doi: 10.22092/ari.2021.355858.1731
T. V Pavlova; N. B Pilkevich; D. V Bessmertnyi; I. A Pavlov; D. V Atiakshin; L. A Pavlova. "Evaluation of New Immunohistochemical Approaches for the Study of Kidney Tumors in Geriatric". سامانه مدیریت نشریات علمی, 76, 4, 1400, 1107-1113. doi: 10.22092/ari.2021.355858.1731
Pavlova, T. V, Pilkevich, N. B, Bessmertnyi, D. V, Pavlov, I. A, Atiakshin, D. V, Pavlova, L. A. (1400). 'Evaluation of New Immunohistochemical Approaches for the Study of Kidney Tumors in Geriatric', سامانه مدیریت نشریات علمی, 76(4), pp. 1107-1113. doi: 10.22092/ari.2021.355858.1731
Pavlova, T. V, Pilkevich, N. B, Bessmertnyi, D. V, Pavlov, I. A, Atiakshin, D. V, Pavlova, L. A. Evaluation of New Immunohistochemical Approaches for the Study of Kidney Tumors in Geriatric. سامانه مدیریت نشریات علمی, 1400; 76(4): 1107-1113. doi: 10.22092/ari.2021.355858.1731

Evaluation of New Immunohistochemical Approaches for the Study of Kidney Tumors in Geriatric

Archives of Razi Institute
مقاله 41، دوره 76، شماره 4، آذر و دی 2021، صفحه 1107-1113    اصل مقاله (563.79 K)
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22092/ari.2021.355858.1731
نویسندگان
T. V Pavlova* 1؛ N. B Pilkevich1؛ D. V Bessmertnyi1؛ I. A Pavlov2؛ D. V Atiakshin3؛ L. A Pavlova1
1Department of Pathology, Belgorod State University, Belgorod, Russian Federation
2Urological Department, Belgorod Cancer Clinic, Belgorod, Russian Federation
3Voronezh State Medical Academy Named after N. N. Burdenko, 394036, Voronezh, Russian Federation
چکیده
Kidney malignancies are among the most deadly genitourinary tumors. It is more common in males and is often seen in people aged 60-70 years old. The incidence rate of kidney cancer seems to be increasing. One reason for this may be the fact that imaging techniques, such as computed tomography scans are more commonly used. These tests may lead to the accidental detection of more kidney cancers. Fortunately, kidney cancer is often detected in the early stages, when the tumor is small and confined to the kidney. The objective of this study was the development of new diagnostic immunohistochemical methods. Clinical examination material of 134 people, including 94 (70%) males and 40 (30%) females, were used in this study. Immunohistochemical staining of tryptase was carried out in compliance with the requirements using Anti-Mast Cell Tryptase antibodies. Goat anti-mouse antibodies #AS-M1-HRP were used as secondary antibodies, visualized with ImmPACTTM DAB Peroxidase Substrate Kit (#SK-4105) according to the instructions of the manufacturer. The nuclei were counterstained with Mayer's hematoxylin, and the sections were embedded in a permanent mounting medium. The immunohistochemical study showed an increase in both tryptase- and chymase-positive mast cells in the renal parenchyma, compared with the control group. The number of mast cells with tryptase expression directly in the tumor was significantly less than the peritumoral localization. A similar pattern was observed for chymase-positive mast cells as the content of the tumor was more than 10times higher than the intratumoral arrangement. The histological and immunological characteristics did not differ in different age groups. The immunohistochemical method of research in the diagnosis of renal tumors plays an important diagnostic and prognostic value. It can assist pathologists in difficult and ambiguous cases to correctly diagnose renal tumors. This will make it possible to prescribe the correct treatment and predict the course of malignant tumor growth in patients.
کلیدواژه‌ها
Kidneys؛ Metastases؛ Oncourology؛ Trace elements
سایر فایل های مرتبط با مقاله
مراجع
  1. top-10-deadliest-diseases [Available from: https://www.healthline.com/health/top-10-deadliest-diseases.
  2. Baumgarten M, Gehr TW, D. C. Diseases of the Kidney 99. Family Med. 2017.
  3. Di Carlo A. Evaluation of neutrophil gelatinase-associated lipocalin (NGAL), matrix metalloproteinase‑9 (MMP‑9) and their complex MMP‑9/NGAL in sera and urine of patients with kidney tumors. Oncol Lett. 2013;5(5):1677-81.
  4. Green T, Odum J, Nash JA, Foster JR. Perchloroethylene-induced rat kidney tumors: an investigation of the mechanisms involved and their relevance to humans. Toxicol Appl Pharmacol. 1990;103(1):77-89.
  5. Chudzinski-Tavassi AM, Morais KLP, de Souza JG, Chammas R. Role of Immune System in Kidney Cancer. Evolving Trends in Kidney Cancer: Intech Open; 2018. p. 291-303.
  6. Drake CG, Stein MN. The immunobiology of kidney cancer. J Clin Oncol. 2018;36(36):3547-52.
  7. McCarthy CE, White JM, Viola NT, Gibson HM. In vivo Imaging Technologies to Monitor the Immune System. Front Immunol. 2020;11:1067.
  8. Mcdougal WS, Kursh ED, Persky L. Spontaneous rupture of the kidney with perirenal hematoma. J Urol. 1975;114(2):181-4.
  9. Rafferty KA. Spontaneous kidney tumors in the frog: rate of occurrence in isolated adults. Science. 1963;141(3582):720-1.
  10. Pavlova T, Pavlov I, Pilkevich N, Chaplyginа M. New approaches in the diagnosis of kidney cancer. Drug Invent Today. 2019;11(9):2094-9.
  11. Raaschou‐Nielsen O, Pedersen M, Stafoggia M, Weinmayr G, Andersen ZJ, Galassi C, et al. Outdoor air pollution and risk for kidney parenchyma cancer in 14 European cohorts. Int J Cancer 2017;140(7):1528-37.
  12. Dugué PA, Bassett JK, Joo JE, Jung CH, Ming Wong E, Moreno‐Betancur M, et al. DNA methylation‐based biological aging and cancer risk and survival: Pooled analysis of seven prospective studies. Int J Cancer. 2018;142(8):1611-9.
  13. Atiakshin D, E B. Mast cells of jejunum in Mongolian gerbils after space flight. J Anat Histopathol. 2014;3:15-27.
  14. Buchwalow IB, Böcker W. Immunohistochemistry. Basics Methods. 2010;1:1-149.
  15. Anker P, Mulcahy H, Chen XQ, Stroun M. Detection of circulating tumour DNA in the blood (plasma/serum) of cancer patients. Cancer Metastasis Rev. 1999;18(1):65-73.
  16. Presti Jr JC, Moch H, Reuter VE, Huynh D, Waldman FM. Comparative genomic hybridization for genetic analysis of renal oncocytomas. Genes Chromosomes Cancer. 1996;17(4):199-204.
  17. Silk MT, Mikkilineni N, Silk TC, Zabor EC, Ostrovnaya I, Hakimi AA, et al. Prospective evaluation of unprocessed core needle biopsy DNA and RNA yield from lung, liver, and kidney tumors: Implications for cancer genomics. Anal Cell Pathol. 2018;2018.
  18. Merino MJ, Torres-Cabala C, Pinto P, Linehan WM. The morphologic spectrum of kidney tumors in hereditary leiomyomatosis and renal cell carcinoma (HLRCC) syndrome. Am J Surg Pathol. 2007;31(10):1578-85.
  19. Motzer RJ, Bander NH, Nanus DM. Renal-cell carcinoma. N Engl J Med. 1996;335(12):865-75.
  20. Atiakshin D, Buchwalow I, Samoilova V, Tiemann Tryptase as a polyfunctional component of mast cells. Histochem Cell Biol. 2018;149(5):461-77.
  21. Atiakshin D, Buchwalow I, Tiemann M. Mast cell chymase: morphofunctional characteristics. Histochem Cell Biol. 2019;152(4):253-69.
  22. Atiakshin D, Samoilova V, Buchwalow I, Boecker W, Tiemann M. Characterization of mast cell populations using different methods for their identification. Histochem Cell Biol. 2017;147(6):683-94.
  23. Buchwalow I, Boecker W, Tiemann M. The contribution of Paul Ehrlich to histochemistry: a tribute on the occasion of the centenary of his death. Virchows Archiv. 2015;466(1):111-6.
  24. Buchwalow I, Samoilova V, Boecker W, Tiemann M. Non-specific binding of antibodies in immunohistochemistry: fallacies and facts. Sci Rep. 2011;1(1):1-6.
آمار
تعداد مشاهده مقاله: 1,504
تعداد دریافت فایل اصل مقاله: 2,467


counter
سامانه مدیریت نشریات علمی. طراحی و پیاده سازی از سیناوب