| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شمارهها | 3,168 |
| تعداد مقالات | 35,197 |
| تعداد مشاهده مقاله | 50,932,154 |
| تعداد دریافت فایل اصل مقاله | 90,937,540 |
بررسی صفات فیزیولوژیک، درصد و اجزای اسانس دو زیرگونه مرزنجوش (Origanum vulgare subsp. gracile و O. vulgare subsp. hirtum) تحت تنش شوری | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 3، دوره 37، شماره 4 - شماره پیاپی 108، مهر و آبان 1400، صفحه 563-583 اصل مقاله (1.45 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2021.352906.2917 | ||
| نویسندگان | ||
| زهرا ابراهیمی1؛ محمدرضا مرشدلو* 2؛ محمد باقر حسن پور اقدم3 | ||
| 1دانشجوی کارشناسی ارشد گیاهان دارویی، دانشکده کشاورزی، دانشگاه مراغه، مراغه، ایران | ||
| 2استادیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه مراغه، مراغه، ایران | ||
| 3دانشیار، گروه علوم باغبانی، دانشکده کشاورزی، دانشگاه مراغه، مراغه، ایران | ||
| چکیده | ||
| بهمنظور بررسی اثرات سطوح مختلف شوری بر محتوای اسانس و برخی خصوصیات مورفولوژیکی و فیزیولوژیکی دو زیر گونه مرزنجوش بخارایی (Origanum vulgare subsp. gracile (K.Koch) letsw.) و یونانی (O. vulgare subsp. hirtum (Link) letsw.) آزمایشی گلخانهای بهصورت فاکتوریل بر پایه طرح کاملاً تصادفی با 4 تیمار و3 تکرار اجرا شد. تیمار تنش شوری با استفاده از کلرید سدیم در محلول غذایی هوگلند و در چهار سطح شاهد (بدون تنش)، و تنشهای ملایم (شوری 30 میلیمولار)، متوسط (شوری 60 میلیمولار) و شدید (شوری 90 میلیمولار) اعمال گردید. براساس نتایج، تحت تنش شوری شاخصهای رشدی، کاروتنوئید کل و شاخص کلروفیل کاهش یافتند، اما فعالیت آنزیمهای آنتیاکسیدانی و غلظت مالوندیآلدئید و پراکسید هیدروژن در هر دو زیرگونه افزایش پیدا کردند. وزن تر و خشک، کاروتنوئید، پراکسیدهیدروژن و فعالیت آنزیم گایاکولپراکسیداز در مرزنجوش یونانی بیشتر از زیر گونه بخارایی بدست آمد. درصد و اجزای اسانس نیز تحتتأثیر شوری قرار گرفتند. با افزایش شوری، درصد اسانس نسبت به شاهد افزایش یافت و بیشترین مقدار آن (0.8%) در تنش ملایم بدست آمد. 35 ترکیب از دو زیرگونه مرزنجوش شناسایی شد. کارواکرول (72-30%: کارواکرول زیرگونه یونانی بیشتر از بخارایی)، گاما-ترپینن، کارواکرول متیلاتر و پارا-سیمن ترکیبهای غالب هر دو زیرگونه بودند. نتایج این پژوهش برتری مرزنجوش یونانی نسبت به بخارایی را از لحاظ تحمل به تنش شوری و همچنین عملکرد کمّی و کیفی نشان داد. | ||
| کلیدواژهها | ||
| تنش غیرزیستی؛ رنگیزههای فتوسنتزی؛ شاخص مورفولوژیکی؛ شاخص فیزیولوژیکی؛ کارواکرول | ||
| عنوان مقاله [English] | ||
| Study on physiological traits, percentage and essential oil components of two oregano subspecies (Origanum vulgare subsp. gracile (K.Koch) letsw. and O. vulgare subsp. hirtum (Link) letsw.) under salinity stress | ||
| نویسندگان [English] | ||
| Z. Ebrahimi1؛ M.R. Morshedloo2؛ M.B. Hassanpour Aghdam3 | ||
| 1M.Sc student, Department of Horticulture Science, Faculty of Agriculture, University of Maragheh, Maragheh, Iran | ||
| 2Department of Horticulture Science, Faculty of Agriculture, University of Maragheh, Iran | ||
| 3Maragheh of Horticulture Science, Faculty of Agriculture, University of Maragheh, Maragheh, Iran | ||
| چکیده [English] | ||
| To investigate the effects of different salinity levels on the essential oil content, and some morphological and physiological characteristics of two oregano subspecies (Origanum vulgare subsp. gracile (K.Koch) letsw. and O. vulgare subsp. hirtum (Link) letsw.), a factorial greenhouse experiment was conducted in a completely randomized design with four treatments and three replications. The salinity stress treatment was applied using sodium chloride in Hoagland nutrient solution at four levels of control (no stress), and mild (salinity 30 mM), moderate (salinity 60 mM), and severe (salinity 90 mM) stresses. According to the results, under salinity stress the growth indices, total carotenoids, and chlorophyll index decreased, but the activity of antioxidant enzymes and concentration of malondialdehyde and hydrogen peroxide increased in both subspecies. The wet and dry weight, carotenoids, hydrogen peroxide, and guaiacol peroxidase activity were obtained higher in subsp. hirtum than subsp. gracile. The percentage and essential oil composition were also affected by salinity. With increasing the salinity levels, the essential oil percentage was significantly increased compared to the control and its highest amount (0.8%) was observed under mild stress. Thirty five compounds were identified in both oregano subspecies essential oil. Carvacrol (30-72%: subsp. hirtum crarvacrol more than the other subsp.), γ-terpinene, carvacrol methyl ether, and p-cymene were the main components of both subspecies. The results of this study demonstrated the superiority of subsp. hirtum to the other oregano subspecies in terms of the tolerance to salinity stress and also quantitative and qualitative yields. | ||
| کلیدواژهها [English] | ||
| Abiotic stress, photosynthetic pigments, morpho-physiological traits, carvacrol | ||
| مراجع | ||
|
- Adams, R.P., 2001. Identification of Essential Oil Component by Gas Chromatography/Mass Spectroscopy. Illinois: Allureed Publication, 804p. - Afshar, M. and Lanad Moghadam, A.R., 2015. Evaluation the effect of salicylic acid on some quantitative, qualitative and growth on salt stress in basil (Ocimum basilicum L.) plant. Journal of Cell and Molecular Research, 28(5): 36-43. - Akbari, S., Kordi, S. and Ghanbari, F., 2016. Physiological responses of summer savory (Satureja hortensis L.) under salinity stress. International Journal of Agriculture and Crop Sciences, 5(2): 1702-1708. - Alaei, Y., Kazemitabar, S.K., Zaefi Zadeh, M., Najafi Zarini, H. and Kiani, G., 2020. A study on the salinity stress effects on the biochemical traits of seedlings and its relationship with resistance toward sensitive and tolerant flax genotypes. Journal of Applied Biology & Biotechnology, 8(1): 47-52. - Arnon, A.N., 1967. Method of extraction of chlorophyll in the plants. Argonomy Journal, 23: 112-121. - Arvin, P., 2015. Effect of gibberellin on some morphological traits, photosyrthetic pigments content and proline in savoy (Satureja hortensis L.) under salinity stress conditions. Journal of Crop Production Research (Environmental Stresses in Plant Sciences), 7(2): 89-104. - Askary, M., Talebi, S.M., Amini, F. and Bangan, A.D.B., 2017. Effects of iron nanoparticles on Mentha piperita L. under salinity stress. Biologija, 63(1): 65-75. - El-Din, A.A.E., Aziz, E.E., Hendawy, S. and Omer, E.A., 2009. Response of Thymus vulgaris L. to salt stress and Alar (B9) in newly reclaimed soil. Journal of applied Sciences Research, 59(12): 2165-2170. - Baatour, O., Nasri-Ayachi, M.B., Mahmoudi, H., Tarchoun, I., Nassri, N., Zaghdoudi, M., Abidi, W., Kaddour, R., Hamdaoui, G., Marzouk, B. and Lachaal, M., 2012. Salt effect on physiological, biochemical and anatomical structures of two Origanum majorana varieties (Tunisian and Canadian). African Journal of Biotechnology, 11(27): 7109-7118. - Baher, Z.F., Mirza, M., Ghorbanli, M. and Rezaii, M.B., 2002. The influence of water stress on plant height, herbal and essential oil yield and composition in Satureja hortensis L. Flavour and Fragrance Journal, 17(4): 275-277. - Ben Tarrit, M., Msaada, K., Hosni, K., Hammami, M., Kchouk, M.E. and Marzouk, B., 2009. Plant growth essential oil yield and composition of sage (Salvia officinalis L.) fruits cultivated under salt stress conditions. Industrial Crops and Products, 30(3): 333-337. - Bernstein, N., Kravchik, M. and Dudai, N., 2010. Salinity-induced changes in essential oil, pigments and salts accumulation in sweet basil (Ocimum basilicum) in relation to alteration of morphological development. Annals of Applied Biology, 156: 167-177. - Burbott, A.J. and Loomis, W.D., 1969. Evidence for metabolic turnover of monoterpenes in peppermint. Plant Physiology, 44(2), pp.173-179. - Crocoll, C., Asbach, J., Novak, J., Gershenzon, J. and Degenhardt, J., 2010. Terpene synthases of oregano (Origanum vulgare L.) and their roles in the pathway and regulation of terpene biosynthesis. Plant Molecular Biology, 73: 587-603. - Deghani, R., Radjabian, T., Talei, D. and Saboora, A., 2015. Effects of salt stress on the pigment content of medicinal plant lemon balm (Mellissa officinalis L.). 4th National Congress on Medicinal Plants, Tehran, 12-13 May: 552. - Dermiral, T. and Turkan, I., 2005. Comparative lipid peroxidation, antioxidant defense system and proline content in root of two rice cultivars differing in salt tolerance. Environmental and Expreimental Botany, 53(3): 247-257. - El-Esawi, M., Elansary, H., El-shanhorey, N. and Elshikh, M., 2017. Salicylic acid-regulated antioxidant mechanisms and Gene expression enhance Rosemary performance under saline conditions. Frontiers in Physiology, 8(716): 1-14. - Emami Bistgani, Z., Hashemi, M., Dagosta, M., Craker, L., Maggi, F. and Morshedloo, M.R., 2019. Effect of salinity stress on the physiological characteristics, phenolic compounds and antioxidant activity of Thymus vulgaris L. and Thymus daenensis Celak. Industrial Crops and Products, 135: 311-320. - Farsaraei, S., Moghaddam, M. and Ghasemi, A., 2020. Changes in growth and essential oil composition of sweet basil in response of salinity stress and superabsorbents application. Scientia Horticulturae, 271: 109625. - Filho, H.G., Silva, P.C.C., da Silva, M.G., Pereira, M.M., Soares, T.M., Neto, A.D., Vasconcelos, R.S. and Gheyi, H.R., 2018. Growth, production and essential oil content of basil genotypes in hydroponic conditions under salt stress. Journal of Experimental Agriculture International, 25(1): 1-10. - Gengmo, Z., Quanmei, S.H., Yu, H., Shihui, L. and Changhai, W., 2014. The physiological and biochemical responses of a medicinal plant (salvia miltiorrhiza L.) to stress caused by various concentration of NaCl. Plos One, 9(2): 1-6. - Ghorbani, M., Movahhedy, Z., Kheiry, A. and Rostami, M., 2018. Effect of salinty stress on some morpho-physiological traits and quantity and quality of essential oil in peppermint (Mentha piperita L.). Environmental Stresses in Crop Sciences, 11(2): 413-420. - Gohari, G.R. and Bahrami, M.K., 2020. Effects of chitosan as growth elictor on some growth parameters and essential oils yield of Dracocephalium moldavica L. under salinity condition. Journal of Agricultural Science, 30(1): 155-169. - Gupta, B. and Hung, B., 2014. Mechanism of salinity tolerance in plants: physiological, biochemical, and moleculare characterization: a review. International Journal of Genomics, 2014: 701596. - Hendawy, S.F. and Khalid, K.H.A., 2005. Response of sage (Salvia officinalis L.) plants to zink application under different salinity levels. Applied Science and Research, 1: 147-155. - Hojjat nooghi, F. and Mozaffari, V., 2012. Effect of calcium on eliminating the negative effect of salinity in pistachio (pistacia vera L.) seedling. Australian Journal of Crop Science, 6(4): 711-716. - Hussain, K., Majeed, A., Nawaz, K., Khizar Hayat, B. and Nisar, M.F., 2009. Effect of different levels of salinity on growth and ion contents of black seeds (Nigella sativa L.). Current Research Journal of Biological Sciences, 1: 135-138. - Husseini, H., Mousavi-Fard, S., Fatehi, F. and Qaderi, A., 2016. Changes in phytochemical and morpho-physilogical traits of thyme (Thymus vulgaris cv Varico3) under different salinity levels. Journal of Medicinal plants, 16(10): 22-33. - Jahantigh, O., Najafi, F., Naghdi Badi, H.A., Khavari Nejad, R.A. and Sanjarian, F., 2016. Study of some physiological parameters hyssop (Hyssopus officinalis) in the vegetative stage under the influence of salinity. Iranian Journal of Plant Biology, 27(1): 81-94. - Jaleel, C.A., Gopi, R. and Panneerselvam, R., 2008. Growth and photosynthetic pigments responses of two varieties of Catharantus roseus to triadim. Comptes Rendus Biologies, 331(4): 272-277. - Jelali, N., Dhifi, W., Chahed, T. and Marzouk, B., 2011. Salinity effects on growth, essential oil yield and composition and phenolic compounds content of marjoram (Origanum Majorana L.) leaves. Journal of Food Biochemistry, 35(5): 1443-1450. - Khalid, K., Jamil, A. and Teixeirada, S., 2010. Yield, essential oil and pigment content of ethnopharmacol. Scientia Horticulturae, 126(2): 297-305. - Khalvandi, M., Ameriana, M., Pridashti, H., Keramati, S. and Hosseini, J., 2019. Essential oil of peppermint in symbiotic relation ship with Piriformospora indica and methyl jasmonate application under saline condition. Industrial Crops and Products, 127: 195-202. - Merati, M.J., Niknam, V., Hassanpour, H. and Mirmasoumi, M., 2015. Comparative effects of salt stress on growth and antioxidative responses in different organs of pennyroyal (Mentha pulegium L.). Journal of Plant Researches, 28(5): 1097-1106. - Mirmohammady meybodi, A. and Gharyazi, M.B., 2010. The salinity stress of aspects physiological and corrective in crops. Isfahan University of Technology, 288p. - Morshedloo, M.R., Salami, S.A., Nazeri, V., Maggi, F. and Craker, L., 2018. Essential oil profile of oregano (Origanum vulgare L.) populations grown under similar soil and climate conditions. Industrial Crops and Products, 119: 183-190. - Morshedloo, M.R., Ahmadi, H., Pirali Hamadani, M. and Yazdani, D., 2017a. An over review to Origanum vulgare L. and its pharmacological properties. Journal of Medicinal Plants, 17(68): 15-31. - Morshedloo, M.R., Salami, S.A., Nazeri, V. and Craker, L.E., 2017b. Prolonged water stress on growth and constituency of Iranian of Oregano (Origanum vulgare L.). Journal of Medicinally Active Plants, 5(2): 7-19. - Morshedloo, M.R., 2016. Evaluation of Physiological, Phytochemical and Gene Expression Pathway of Iranian Oregano (Origanum vulgare L.) Under Water Deficit Conditions. Ph.D. Dissertation, University of Tehran. - Morshedloo, M.R., Ebadi, A., Maggi, F., Fattahi, R., Yazdani, D. and Jafari, M., 2015. Chemical characterization of the essential oil compositions from Iranian populations of Hypericum perforatum L. Industrial Crops and Products, 76: 565-573. - Mohammadkhani, N. and Heidari, R., 2007. Effects of drought stress on protective enzyme Activities and lipid peroxidation in two maize cultivars. Pakistan Journal of Biological Sciences, 10(21): 3835-3840. - Mohammadizadeh, H.A., Mirzakhani, G., Ghafai, M., Samavatipour, P., Araghi, S.M. and fatehi, M.F., 2014. Effect of Nacl stress on seed germination indices and early scedling growth of cumin (Cuminnum cyminum L.) an important medicinal plant. Agricultural Science Development, 3(2): 161-166. - Mozaffarian, V., 2012. Ibntification of Medicinal and Aromatic Plants of Iran. Farhang Moaser Press, Tehran, Iran, 1350p. - Najafi, F., Khavari-nejad, R.A. and Siahali, M., 2010. The effects of salt stress on certain physidogical parameters in summer savory Satureja hortensis L. Plant Journal of Stress Physology and Biochemistry, 6(1): 13-21. - Olfa, B., Zaghdoudi, M., Bensalem, N. and Ouerghi, Z., 2018. Effects of NaCl on plant growth and antioxdant activites in fenugreek (Trigonella foenum graecum L.). Bioscience Journal, 34(3): 683-696. - Omidbaigi, R., 2011. Production and Processing of Medicinal Plants (Vol. 2). Astan Qods Razavi Publication, 440p. - Oueslati, S., Karry-Bouraouri, N., Attia, H., Rahbi, M., Ksouri, R. and Lachaal, M., 2010. Physiological and antioxidant responses of Mentha pulegium (Pennyroyal) to salt stress. Acta Physiologiae Plantarum, 32: 289-296. - Ozturk, A., Unlukara, A., Ipek, A. and Gurbuz, B., 2004. Effect of salt stress and water deficit on plant growth and essential oil content of lemon balm (Melissa officinalis L.). Pakistan Journal of Botany, 36(4): 787-792. - Roodbari, N., Roodbari, Sh., Ganjali, A., Nejad, F. and Ansarifar, M., 2013. The effect of salinity stress on growth parameters and essential oil percentage of peppermint (Mentha piperita L.). International Journal of Basic and Applied Sciences, 2(1): 294-299. - Said-Al Ahl, H.A.H., Abou-Ellail, M. and Omer, E.A., 2016. Harvest date and genotype influences growth characters and essential oil production and composition of petroselinum crispum plants. Journal of Chemical and Pharmaceutical Research, 2016(85): 992-1003. - Said-Al Ahl, H.A.H. and Omer, E.A., 2011. Medicinal and aromatic plant production under salt stress: a review. Kerld Polonica, 57: 72-87. - Sairam, R.K. and Tyagi, A., 2004. Physiology and molecular biology of salinity stress tolerancein plants. Current Science, 86: 407-421. - Tholl, D., 2015. Biotechnology of isoprenoids: Biosynthesis and biological functions of terpenoids in plants. Advances in Biochemical Engineering/Biotechnology, 148: 63-106. - Vaidyanathan, H., Sivakumar, P., Chakrabarty, R. and Thomas, G., 2003. Scavenging of reactive oxygen species in NaCl-stressed rice (Oriza sativa L.) differential response in salt tolerant and sensitive varieties. Plant Science, 165: 1411-1418. - Valifard, M., Mohsenzadeh, S., Kholdebrain, B. and Rowshan, V., 2014. Effect of salt stress on volatile compoundes of Salvia mirzayanii. South African Journal of Botany, 93: 92-97. - Verpoorte, R., Contin, A. and Memelink, J., 2002. Biotechnology for the production of plant secondary metabolites. Phytochemistry, 1: 13-25. - Weisany, W., Sohrabi, Y., Heidari, Gh., Siosemardeh, A. and Ghassemi-Golezani, K., 2012. Changes in antioxidant enzymes activity and plant performance by salinity stress and zinc applicaton in soybean (Glycine max L.). Plant Omis Journal, 5: 60-67. - Zhang, J.L. and Shi, H., 2013. Physiological and molecular mechanisms of plant salt tolerance. Photosynthesis Research, 116: 1-22. - Zhou, Y., Tang, N., Huang, L., Zhao, Y., Tang, X. and Wang, K., 2018. Effects of salt stress on plant growth, antioxidant capicity, glandular trichome density, and volatile exudates of Schizonepeta tenuifolia Briq. International Journal of Moleculare Sciences, 19(252): 2-15. - Zrig, A., Tounektia, T., Abd Elgawad, H., Hegab, M.M., Oueled Alia, S. and Khemir, H., 2016. Essential oils, amino acids and polyphenols changes in salt- stress Thymus vulgaris exposed ti open field and shade enclosure. Industrial Crops and Products, 91: 223-230. | ||
|
آمار تعداد مشاهده مقاله: 1,703 تعداد دریافت فایل اصل مقاله: 669 |
||