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Enhanced Production of Thebaine in Cell Suspension Cultures of Papaver bracteatum by overexpression of SalAT gene | ||
| Journal of Medicinal plants and By-products | ||
| دوره 10، Special، تیر 2021، صفحه 69-75 اصل مقاله (187.14 K) | ||
| نوع مقاله: Research Article | ||
| شناسه دیجیتال (DOI): 10.22092/jmpb.2020.128516.1158 | ||
| نویسندگان | ||
| Ardeshir Qaderi؛ Nasrin Qavami؛ Sara Ghezelbash؛ Nassim Zarinpanjeh* | ||
| Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran | ||
| چکیده | ||
| The Iranian poppy by the scientific name of Papaver bracteatum Lindl. has been long propounded as a source for thebaine. Thebaine is among secondary metabolites that due to its wide spread and unique medicinal properties play a valuable role in production of herbal medicines and pharmaceutical industries on the whole.The objective of the present study is to investigate the enhanced production of thebaine by over expression of salutaridinol 7-o-acetyltransferase (SalAT) gene, a key gene in thebaine biosynthesis pathway, in Agorobacterium-mediated transformed cell suspension culture of P. bracteatum. In this study, the cDNA of SalAT with P. bracteatum origin was cloned in pBI121 expression vector under CaMV35s promoter. In order to transfer the cloned gene Agrobacterium tumefaciens of LBA4404 strain and hypocotyl–cotyledon explants were used. For recognition of transformed calli obtained, PCR and Southern blot analysis were employed and high performance liquid chromatography analysis (HPLC) was applied for determination of thebaine content. The comparison of callus induction and thebaine content between transformed and non-transformed cell culture was studied on completely randomized design with three replications. According to HPLC analysis which revealed a significant 1.7 fold (0.58 mg/g) enhancement in thebaine content in transformed suspension cell culture comparing to non-transformed cell culture (0.34 mg/g), it can be concluded that the over expression of key genes such as SalAT gene in the cellular suspension culture of P. bracteatum could be a promising potential for mass production of this valuable alkaloid. | ||
تازه های تحقیق | ||
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| کلیدواژهها | ||
| Cell suspension culture؛ Over expression؛ Papaver bracteatum؛ SalAT gene؛ Thebaine | ||
| مراجع | ||
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1. Gorothe T, Lenz R, Kutchan TM. Molecular Characterization of the Salutaridinol 7-O-Acetyltransferase Involved in Morphine Biosynthesis in Opium Poppy Papaver somniferum .J Biol Chem. 2001;276:30717-30723.
2. Kamimura S, Akutsu M, Nishikawa M. Formation of thebaine in the suspension culture of Papaverbracteatum. Agric Biol Chem.1976;40:913-919.
3. Milo J, Levy A, Palevitch D. An alternative raw-the cultivation and breeding of Papaver bracteatum. In: Bernáth J, editor. Poppy: the Genus Papaver, Harwood Academic Publishers, Amesterdam, 2006, pp. 279-289.
4. Tisserat B, Berhow M. Production of Pharmaceuticals from Papaver Cultivars in Vitro. Eng Life Sci. 2009;3:190-196.
5. Ilahi I, GhauriEG.Regeneration in cultures of Papaver bracteatum as influenced by growth hormones and temperature. Plant Cell Tiss. Organ Cult. 1994;38:81-83.
6. Kamo K, Mahlberg PG.Morphinan Alkaloids: Biosynthesis in Plant (Papaver spp.) Tissue Culture, Medicinal and aromatic plants, Springer-Verlag, Berlin, 1988, pp. 251-263.
7. Smetanska I. Production of Secondary Metabolites Using Plant Cell Cultures, Food Biotechnology, Springer- Verlag, Berlin Heidelberg, 2008, pp. 187-228.
8. Berényi S, Csutorás C, Sipos A. Recent Developments in the Chemistry of Thebaine and its Transformation Products as Pharmacological Targets, Current Medicinal Chemistry.Bentham Sci Publishers. 2009;16:3215-3242.
9. Namdeo AG. Plant Cell Elicitation for Production of Secondary Metabolites: A Review, Phcog. Rev. 2007;1:69-79.
10. Sambrook J, Russell DW. Molecular Cloning, A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York. 2001.
11. Larkin PJ, Miller JA, Allen RS, Chitty JA, Gerlach WL, Frick S, Kutchan TM, Fist AJ. Increasing morphinan alkaloid production by over‐expressing codeinone reductase in transgenic Papaver somniferum. Plant Biotechnol J. 2007;5:26-37.
12. Murashige T, Skoog F. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 1962;15:473-497.
13. Cho HY, Lee-Parsons CWT, Yoon SYH, Rhee HS, Park JM. Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures. Biotechnol Lett. 2007;29:2001-2005.
14. Narciso JO, Hattori K. Genotypic differences in morphology and ultra-structures of callus derived from selected rice varieties. Philipp Sci Lett. 2010;3:59-65.
15. Verma P, Ahmad Khan S, Mathur AJ, Ghosh S, Shanker K, Kalra A.Improved sanguinarine production via biotic and abiotic elicitations and precursor feeding in cell suspensions of latex-less variety of Papaver somniferum with their gene expression studies and upscaling in bioreactor, Protoplasma. 2014;251:1359-1371.
16. Bulgakov VP, Tchernoded GK, Mischenko NP, Khodakovskaya MV, Glazunov VP, Radchenko SV, Zvereva EV, Fedoreyev SA, Zhuravlev YN. Effect of salicylic acid, methyl jasmonate, ethephon and cantharidin on anthraquinone production by Rubia cordifolia callus cultures transformed with the rolB and rolC genes. J Biotechnol. 2002;97:213-221.
17. Ramachandra Rao S, Ravishankar GA. Plant cell cultures: chemical factories of secondary metabolites. Biotechnol Adv. 2002;20:101-153.
18. Gadzovska S, Maury S, Delaunay A, Spasenoski M, Joseph C, Hagege D. Jasmonic acid elicitation of Hypericum perforatum L. cell suspensions and effects on the production of phenylpropanoids and naphtodianthrones. Plant Cell Tissue Org. 2007;89:1-13.
19. McCoy ES, O’Connor E, Natural products from plant cell cultures, Progress in Drug Research, Birkhäuser Basel publication, 2008, pp.331-370.
20. Kumar V, Desai D, Shriram V. Enhanced benzophenanthridine alkaloid production and protein expression with combined elicitor in Eschscholtzia californica suspension cultures, Nat. Prod Bioprospect. 2014;4:107-112.
21. KowalczykT, WieczfinskaJ, SkałaE, SliwinskiT, Sitarek P. Transgenesis as a tool for the efficient production of selected secondary metabolites from plant in vitro cultures. Plants. 2020;9:132. doi:10.3390/plants9020132
22. Park SU, Yu M, Facchini PJ. Modulation of berberine bridge enzyme levels in transgenic root cultures of California poppy alters the accumulation of benzophenanthridine alkaloids. Plant Molecular Biology. 2003;51:153-164.
23. Doyle JJ, Doyle JL. Isolation of plant DNA from fresh tissue. Focus. 1990;12:13-15.
24. Suprun AR, Ogneva ZV, Dubrovina AS, Kiselev KV. Effect of spruce PjSTS1a, PjSTS2, or PjSTS3 gene overexpression on stilbene biosynthesis in callus cultures of Vitis amurensis Rupr. Biotechnol. Appl Biochem. 2019;1-7.
25. Zheng J, Zhuang Y, Mao HZ, Jang IC. Overexpression of SrDXS1 and SrKAH enhances steviol glycosidescontent in transgenic Stevia plants. BMC Plant Biol. 2019;19:1-16.
26. Hidalgo D, Martínez-Márquez A, Cusidó R, Bru-Martínez R, Palazón J, Corchete P. Silybum marianum cell cultures stably transformed with Vitis vinifera stilbene synthase accumulate t-resveratrol in the extracellular medium after elicitation with methyl jasmonate or methylated cyclodextrins. Eng Life Sci. 2017;17:686-694.
27. Dong Y, Duan W, He H, Su P, Zhang M, Song G, Fu C, Yu L. Enhancing taxane biosynthesis in cell suspension culture of Taxus chinensis by overexpressing the neutral/alkaline invertase gene. Process Biochem. 2015;50:651-660. | ||
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