| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شمارهها | 3,186 |
| تعداد مقالات | 35,332 |
| تعداد مشاهده مقاله | 51,394,570 |
| تعداد دریافت فایل اصل مقاله | 91,299,861 |
القأ مقاومت به بیماری پژمردگی فوزاریومی گوجهفرنگی از طریق افزایش در تولید آنزیمهای آنتیاکسیدانی و ژنهای مرتبط با بیماریزایی با استفاده از متیل جاسمونات | ||
| آفات و بیماری های گیاهی | ||
| دوره 88، شماره 2 - شماره پیاپی 111، اسفند 1399، صفحه 275-284 اصل مقاله (638.19 K) | ||
| نوع مقاله: بیماریشناسی گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2020.342199.1332 | ||
| نویسندگان | ||
| سید کاظم صباغ* 1؛ مسعود گلستانی اسفند آبادی2؛ محمد رضا سرافراز اردکانی3؛ بنیامین کاظم پور4؛ مرضیه طاهری5 | ||
| 1Department of Biology, Faculty of استاد دانشگاه یزد Science, Yazd University, Safaieh, 89195-742 Yazd | ||
| 2گروه زراعت دانشگاه پیام نور یزد | ||
| 3گروه زیست شناسی دانشکده علوم دانشگاه یزد | ||
| 4دانشجوی کارشناسی ارشد بیوتکنولوژی کشاورزی دانشکده منابع طبیعی و کویر شناسی دانشگاه یزد | ||
| 5کارشناس آزمایشگاه گروه زیست شناسی دانشکده علوم دانشگاه یزد | ||
| چکیده | ||
| د در این تحقیق امکان القای مقاومت سیستمیک به بیماری پژمردگی فوزاریومی ناشی از Fusarium oxysporum fsp. lycopersisi با استفاده از متیل جاسمونات در دو رقم گوجهفرنگی ترمه و کاپیتان بررسی شد. نهالهای گوجه فرنگی با سه غلظت 2/0، 1/0 و صفر در مرحله 2-4 برگی با روش اسپری دستی تیمار و در مرحله 6 برگی با استفاده از عامل بیمارگر، بیماریزایی صورت گرفت. سطح بیان سه ژن npr1، pds و eds1، فعالیت آنزیمهای کاتالاز، پراکسیداز و پلی فنل اکسیداز و میزان فنل کل بهترتیب با استفاده از روش بررسی بیان ژن در زمان واقعی و طیف سنجی انجام گردید. نتایج این تحقیق نشان داد که فعالیت آنزیم ها در ارقام و بازههای زمانی متغییر میباشد. بیشترین میزان فعالیت آنزیم ها در رقم ترمه تحت تنش بیماری مشاهده شد. بیشترین و کمترین سطح بیان بهترتیب برای ژنهای npr1 و pds اتفاق افتاد. نتایج حاصل از این تحقیق حاکی از آن است که متیل جاسمونات قادر به القا مقاومت در گوجه فرنگی بر علیه بیماری پژمردگی فوزاریومی از طریق تغییر در فعالیتهای بیوشیمیایی و مولکولی میباشد. | ||
| کلیدواژهها | ||
| بیان ژن؛ فاکتورهای نسخه برداری؛ متیل جاسمونات؛ مقاومت اکتسابی | ||
| عنوان مقاله [English] | ||
| Induced resistance to fusarium tomato wilting disease throught increase of antioxidant enzymes and pathogenesis –related genes using MethylJasmonat | ||
| نویسندگان [English] | ||
| masoud Golesrtani2؛ Mohammad Rezaa Sarafraz ardakani3؛ benyamin Kazempour4؛ marziyeh Tahei5؛ | ||
| 2Peyamnoor University of Yazd | ||
| 3Department of Biology, Faculty of Science, Yazd University | ||
| 43- MSc student of Biotechnology, Department of Biotechnology, Faculty of Natural Resources of Desert , Yazd niversity | ||
| 51- Department of Biology, Faculty of Science, Yazd University | ||
| چکیده [English] | ||
| In this research, possibility of induced systemic resistance in tow tomato cultivars Termeh and Capitan was assayed by exogenous application of methyl jasmonat (MeJA). Tomato plants were treated with methyl jasmonat at concentration of 0, 0.1 and 0.2 mM by spraying at 2-4 leaf stage and then were inoculated withpathogen agent at 6- leaf stages. Gene expression level of npr1, pds and eds1 genes and catalase, peroxidase, polyphenol oxidase activity and total phenol continent was investigated using qRT-PCR and spectrophotometery methods, repectively. The results of this research showed that enzyme activity was variable in tested cultivars and interval times after inoculation. The highest enzyme activity was observed in Termeh cultivar under disease stress. The highest and lowest expression level was occured in npr1 and pds genes, respectively. These results indicated that methyl jasmonat is able to iduces resistance in tomato against wilting disease throught change on bio-chemical and molecular activities. | ||
| کلیدواژهها [English] | ||
| Acquired resistance, gene expression, methyl jasmonat, transcription factors | ||
| مراجع | ||
|
BABU, A. N., JOGAIAH, S., ITO, S.I., NAGARAJ, A. and TRAN, L. 2015. Improvement of growth, fruit weight and early blight disease protection of tomato plants by rhizosphere bacteria is correlated with their beneficial traits and induced biosynthesis of antioxidant peroxidase and polyphenol oxidase. Plant Science, 231(1): 62-73. BELA, K., HORVÁTH, E., GALLÉ, Á., SZABADOS, L., TARI, I. and CSISZÁR, J. 2015. Plant glutathione peroxidases: emerging role of the antioxidant enzymes in plant development and stress responses. Journal of Plant Physiology, 176: 192-201. BOLOK YAZDI, H.R., SABBAGH, S.K., MAZAHERI, M., SALARI, M. and MOSHTAGHIOUN, S.M. 2018. Virus-induced gene silencing for functional analysis of eds1 gene in tomato infected with Ralstonia solanacearum. Zemdirbyste-Agriculture, 105(4): 357-362. DESMOND, O.J., EDGAR, C.I., MANNERS, J., MACLEAN, D.J., SCHENK, P. M. and KAZAN, K. 2005. Methyl jasmonate induced gene expression in wheat delays symptom development by the crown rot pathogen Fusarium pseudograminearum. Physiological and Molecular Plant Pathology, 67 (3-5): 171-179. DRIDI, W. and BORDENAVE, N. 2020. Pine Bark Phenolic Extracts, Current Uses, and Potential Food Applications: A Review. Current Pharmaceutical Design, 26(4): 1866-1879. EL-KHALLAL, S. M. 2007. Induction and modulation of resistance in tomato plants against Fusarium wilt disease by bioagent fungi (arbuscular mycorrhiza) and/or hormonal elicitors (jasmonic acid & salicylic acid): 2-changes in the antioxidant enzymes, phenolic compounds and pathogen related-proteins. Australia Journal of Basic Applied Science, 1: 717-732. JAITI, F., VERDEIL, J. L. and HADRAMI, I. 2009. Effect of jasmonic acid on the induction of polyphenoloxidase and peroxidase activities in
KIM, H., FONSECA, J.M., CHOI, J.-H. and KUBOTA, C. 2007. Effect of methyl jasmonate on phenolic compounds and carotenoids of romaine lettuce (Lactuca sativa L.). Journal of Agricultural and Food Chemistry, 55: 10366-10372. KUMAR, N. and GOEL, N. 2019. Phenolic acids: Natural versatile molecules with promising therapeutic applications. Biotechnology Reports, 24: e00370. LECOMTE, C., ALABOUVETTE, C., EDEL-HERMANN, V., ROBERT, F. and STEINBERG, C. 2016. Biological control of ornamental plant diseases caused by Fusarium oxysporum: a review. Biological Control, 101: 17-30. LUO, J., RAN, W., HU, J., YANG, X., XU, Y. and SHEN, Q. 2010. Application of bio-organic fertilizer significantly affected fungal diversity of soils. Soil Science Society of America Journal, 74(6): 2039-2048. MURKOWSKI, A. 2001. Heat stress and spermidine: effect on chlorophyll fluorescence in tomato plants. Biologia Plantarum, 44(3): 53-57. OROZCO-CÁRDENAS, M., NARVÁEZ-VÁSQUEZ, J. and RYAN, C.A. 2001. Hydrogen peroxide acts as a second messenger for the induction of defense genes in tomato plants in response to wounding, systemin, and methyl jasmonate. The Plant Cell, 13(1): 179-191. ORZAEZ, D., MIRABEL, S., WIELAND, W.H. and GRANELL, A. 2006. Agroinjection of tomato fruits. A tool for rapid functional analysis of transgenes directly in fruit. Plant physiology, 140(1): 3-11. REN, L., LOU, Y., SAKAMOTO, K., INUBUSHI, K., AMEMIYA, Y. and SHEN, Q. 2010. Effects of arbuscular mycorrhizal colonization on microbial community in rhizosphere soil and Fusarium wilt disease in tomato. Communications in Soil Science and Plant Analysis, 41: 1399-1410. SABBAGH, S., KERMANIZADEH, B., GHOLAMALIZADEH, A. and SIROUSMEHR, A. 2016. Effects of fertilizer treatments on components, performance components and induce resistance to wheat scab disease. Iranian Journal of Filed Crop Science, 47(1): 77-85. (In Persian with English summary). SABBAGH, S., POORABDOLLAH, A., SIROUSMEHR, A. and GHOLAMALIZADE, A. 2017. Bio-fertilizers and Systemic Acquired Resistance in Fusarium Infected Wheat. Journal of Agricultural Science and Technology, 19: 453-464. SABBAGH, S., ROUDINI, M. and PANJEHKEH, N. 2017. Systemic resistance induced by Trichoderma harzianum and Glomus mossea on cucumber damping-off disease caused by Phytophthora melonis. Archives of Phytopathology and Plant Protection, 50(7-8): 375-388. SABBAGH, S., SABBAGH, E., ABKHOO, J. and ZINATI FAKHRABAD, F. 2016. The Effect of Salicylic Acid to Induce Systemic Resistance in Cucumber Plant to Damping- off Disease Caused by Pythium aphanidermatum Appled Researches in Plant Protection, 5(2): 27-43. (In Persian with English summary). SABBAGH, S.K. and VALIZADEH, SH. 2016. Effect of bio-fertilizers on greenhouse cucumber resistant to damping-off disease caused by Pythium aphanidermatum and increase of yield component. Biological Control of Pests and Plant Diseases, 5(1): 111-122. (In Persian with English summary). SMITH, J.L., DE MORAES, C.M. and MESCHER, M.C. 2009. Jasmonate‐and salicylate‐mediated plant defense responses to insect herbivores, pathogens and parasitic plants. Pest Management Science: formerly Pesticide Science, 65(5): 497-503. SOARES, A., SOUZA, T., JACINTO, T. and MACHADO, O. 2010. Effect of methyl jasmonate on antioxidative enzyme activities and on the contents of ROS and H2O2 in Ricinus communis leaves. Brazilian Journal of Plant Physiology, 22(3): 151-158. SULLIVAN, M.L. 2015. Beyond brown: polyphenol oxidases as enzymes of plant specialized metabolism. Frontiers in Plant Science, 5(11): 783. TALAAT, N., SHAWKY, B. and IBRAHIM, A. 2015. Alleviation of drought-induced oxidative stress in maize (Zea mays L.) plants by dual application of 24-epibrassinolide and spermine. Environmental and Experimental Botany, 113(6): 47-58. TARANTO, F., PASQUALONE, A., MANGINI, G., TRIPODI, P., MIAZZI, M. and PAVAN, S. 2017. Polyphenol oxidases in crops: biochemical, physiological and genetic aspects. International Journal of Molecular Sciences, 18(2): 377. VANITHA, S. and UMESHA S. 2008. Variations in defense related enzyme activities in tomato during the infection with bacterial wilt pathogen. Journal of Plant Interactions 3 (1) : 245-253. WANG, K., JIANG, S., PU, T., FAN, L., SU, F. and YE, M. 2019. Antifungal activity of phenolic monoterpenes and structure-related compounds against plant pathogenic fungi. Natural Product Research, 33: (12) 1423-1430. WIERMER, M. 2005. Molecular and spatial characterisation of Arabidopsis EDS1 defence regulatory complexes. Universität zu Köln. ZAMBOUNIS, A., KALAMAKI, M., TANI, E., PAPLOMATAS, E. and TSAFTARIS, A. 2012. Expression Analysis of Defense-Related Genes in Cotton (Gossypium hirsutum) after Fusarium oxysporum f. sp. vasinfectum Infection and Following Chemical Elicitation using a Salicylic Acid Analog and Methyl Jasmonate. Plant Molecular Biology Reporter, 30(3): 225-234. ZEHRA, A., MEENA, M., DUBEY, M., AMIR, M. and UPADHYAY, R. 2017. Synergistic effects of plant defense elicitors and Trichoderma harzianum on enhanced induction of antioxidant defense system in tomato against Fusarium wilt disease. Botanical Studies, 58(1): 1-14.
| ||
|
آمار تعداد مشاهده مقاله: 689 تعداد دریافت فایل اصل مقاله: 578 |
||