| تعداد نشریات | 287 |
| تعداد نشریات سازمان | 85 |
| تعداد شمارهها | 3,159 |
| تعداد مقالات | 35,108 |
| تعداد مشاهده مقاله | 50,527,980 |
| تعداد دریافت فایل اصل مقاله | 90,477,181 |
ارزیابی فنوتیپی، بیماریزایی و مولکولی جدایههای استرپتومایسس بیمارگر سیبزمینی در خراسان رضوی | ||
| آفات و بیماری های گیاهی | ||
| دوره 88، شماره 2 - شماره پیاپی 111، اسفند 1399، صفحه 227-239 اصل مقاله (649.98 K) | ||
| نوع مقاله: بیماریشناسی گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2020.127992.1306 | ||
| نویسندگان | ||
| فریده بی سیار؛ ساره بقائی راوری* ؛ عصمت مهدیخانی مقدم | ||
| گروه گیاه پزشکی، دانشکده کشاورزی، دانشگاه فردوسی مشهد، مشهد، ایران | ||
| چکیده | ||
| اسکب معمولی یکی از بیماریهای مهم باکتریایی سیبزمینی است که تولید بهینه این محصول اقتصادی را بهطورفراگیر محدود میسازد. متعاقب نمونه برداری سالهای 1396 و 1397، پرگنههای پودری با شکلشناسی جنس استرپتومایسس خالصسازی و از نظر ویژگی های فنوتپی، بیماریزایی و ردیابی ژنهای مستقر روی جزیره بیماریزایی شامل txtAB،nec1 و tomA ارزیابی شدند. شکلشناسی پرگنه جدایهها روی محیط عصاره مخمر-مالت-آگار متنوع و جدایههای بیمارگر علایم عدمجوانهزنی، نکروز وکوتولگی را روی گیاهچههای تربچه نشان داده و از نظر فنوتیپی به شش گروه تفکیک شدند. از 20 جدایه بیمارگر روی تربچه، 11 جدایه قادر به ایجاد نکروز ویا فرورفتگی در ورقههای سیبزمینی و هشت جدایه علایم کلروز و یا نکروز را روی برگهای توتون پسازگذشت 10 روز ایجاد نمودند. نتایج بیماریزایی در شرایط گلخانه، بهصورت زخمهای سطحی و یا برجسته با شاخص شدت لکه بین یک تا چهار مشخص شد. قطعه 700 جفتبازی nec1، در شش جدایه از مجموع هشت جدایه منتخب ردیابی شد. درحالیکه ژنهای txt AB و tom A بهترتیب در جدایههای JR-12 و TM-29 تکثیر شدند. براساس نتایج توالییابی 16SrRNA، جدایه TM-29 بهعنوان Streptomyces bottropensis و جدایه JR-12 بهعنوان Streptomyces sp. مشخص شد. بهنظر میرسد جدایههای خراسان رضوی، دارای فاکتور بیماریزایی بهغیر از تاکستومین A هستند که برای تأیید آن، انجام آزمایشات تکمیلی ضرورت دارد. | ||
| کلیدواژهها | ||
| استرپتومایسس؛ بیماریزایی؛ پرآزاری؛ شناسایی مولکولی | ||
| عنوان مقاله [English] | ||
| Phenotypic, pathogenicity and molecular evaluation of potato pathogenic Streptomyces strains in Razavi-Khorasan | ||
| نویسندگان [English] | ||
| Farideh Bisayar؛ Sareh Baghaee-Ravari؛ Esmat Mahdikhani-Moghaddam | ||
| Department of Crop Protection, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran | ||
| چکیده [English] | ||
| Common scab is one of the most important bacterial diseases of potato which restricts the optimal production of this economical crop. Following sampling in 2018-2019, the powdery colonies with Streptomyces morphology were purified and evaluated based on phenotypic features, pathogenicity and detecting the genes on Pathogenicity Island including txtAB, nec1 and tomA. The colony morphology of the strains on malt yeaste extract agar was variable and pathogenic strains showed symptoms such as no germination, dwarfing and necrosis on radish seedlings which were divided into six phenotypic groups. 11 out of 20 pathogenic strains on radish, were capable of causing necrosis or pitting on potato slices and eight of them showed chlorosis or necrosis on tobacco leaves after 10 days. The results of pathogenicity including superficial or raised lesions with the lesion severity index of 1-4 were recorded in greenhouse conditions. The 700 bp fragment was detected in six out of eight selected strains. While, txtAB and tomA were amplified in JR-12 and TM-29, respectively. Based on 16rSRNA sequencing results, JR-12 and TM-29 strains were characterized as Streptomyces bottropensis and Streptomyces sp < em>. Respectively.It seems that the Razavi-Khorasan strains have a virulence factor other than Thaxtomine A, which needs further experiments to confirm. | ||
| کلیدواژهها [English] | ||
| Molecular characterization, pathogenicity, Streptomyces, virulence | ||
| مراجع | ||
|
AITTAMAA, M., SOMERVUO, P., LAAKSO, I., AUVINEN, P. and J. VALKONEN. 2010. Microarray-based comparison of genetic differences between strains of Streptomyces turgidiscabies with focus on the pathogenicity island. Molecular Plant Pathology, 11: 733-746 AUSUBLE, F., BRENT, F.M., KINGESTON, R.E., MOOR, D.D., SMITH, J.A., SEIDEMAN, J.G., and K. STRUH. 1992. Current Protocol in Molecular Biology. Greene. Wiley Interscience. New York, USA. BIGNELL, D. R. D., FYANS, J. K., and CHENG, Z. 2014. Phytotoxins produced by plant pathogenic Streptomyces species. Journal of Applied Microbiology, 116: 223-235. BOUCHEK-MECHICHE, K., GARDAN, L., NORMAND, P. and B. JOUAN. 2000. DNA relatedness among strains of Streptomyces pathogenic to potato in France: description of three new species, S. europaeiscabiei spp. nov. and S. stelliscabiei spp. nov. associated with common scab, and S. reticuliscabiei spp. nov. associated with netted scab. International Journal of Systematic Evolutionary Microbiology, 50: 91-99. BUKHALID, R. A., CHUNG, S. Y. and R. LORIA. 1998. nec1, a gene conferring a necrogenic phenotype, is conserved in plant pathogenic Streptomyces spp. and linked to a transposase pseudogene. Molecular Plant-Microbe Interaction, 11: 960-967. BULHALID, R. A., TAKEUCHI, T., LABEDA, D. and R. LORIA. 2002. Horizontal transfer of the plant virulence gene, nec1, and flanking sequences among genetically distinct Streptomyces strains in the diastatochromogenes cluster. Applied and Environmental Microbiology, 68: 738-744. BULHALID, R. A.and R. LORIA. 1997. Cloning and expression of a gene from Streptomyces scabies encoding a putative pathogenicity factor. Journal of Bacteriology, 179: 7776-7783. CAO, Z., KHODAKARAMIAN, G.H., ARAKAWA, K. and H. KISHANI. 2012. Isolation of borrelidin as a phytotoxic compound from a potato pathogenic Streptomyces strain. Bioscience, Biotechnology, and Biochemistry, 76: 353-357. CULLEN, D.W. and A.K. LEES. 2007. Detection of the nec1 virulence gene and its correlationwith pathogenicity in Streptomyces species on potato tubers and in soil using conventional and real-time PCR. Journal of Applied Microbiology 102, 1082–94 DEES, M., SOMERVUO, P., LYSOE, E., AITTAMAA, M. and J. Valkpnen. 2012. Species identification and microarray-based comparative genome analysis of Streptomyces species isolated from potato scab lesions in Norway. Molecular Plant Pathology, 13: 174-186. EINI, O., KHODAKARAMIAN, G.H. and H. RAHIMIAN. 2003. Phenotypic features and host range of the strains of Streptomyces inducing potato scab in Iran. Iranian Journal of Plant Pathology, 39: 85-101. ERRAKHI, R., DAUPHIN, A., MEIMOUN, P., LEHNER, A., REBOUTIER, D., VATSA, P., BRIAND, J., MADIONA, K., RONA, J. P., BARAKATE, M., WENDEHENNE, D., BEAULIEU, C. and F. BOUTEAU. 2008. An early Ca2+ influx is a prerequisite to thaxtomin A-induced cell death in Arabidopsis thaliana cells. Journal of Experimental Botany, 59: 4259-4270. FADAEE-TEHRANI, A.A. and A. JAFARI. 2016. Phenotypic diversity of potato common scab strains from Chaharmahal-Bakhtiyari province. Journal of Plant Protection. 30:31-41. FLORES-GONZALES, R., VELASCO, I. and F. MONTES. 2008. Detection and characterization of Streptomyces causing potato common scab in Western Europe. Plant Pathology, 57: 162–169.
FYANS, J. k., BOWN, L. and D. R. D. BIGNELL. 2016. Isolation and Characterization of Plant-Pathogenic Streptomyces species Associated with Common Scab-Infected Potato Tubers in Newfoundland. Phytopathology, 106: 123-131. HAO, J., MENG, Q., YIN, J. and W. KIRK. 2009. Characterization of new Streptomyces strain, SD3024, that causes potato common scab. Plant Disease, 93: 1329-1334. HASSANI, S. and S. M. TAGHAVI. 2014. Phenotype and genotype diversity of Iranian streptomyces isolates that cause potato common scab. Journal of Plant Pathology, 96: 467-476. HEALY, F.G., WACH, M., KRASNOFF, S. B., GIBSON, D. M. and R. LORIA. 2000. The txtAB genes of the plant pathogen Streptomyces acidiscabies encode a peptide synthetase required for phytotoxin thaxtomin a production and pathogenicity. Molecular Microbiology, 38: 794-804. JOSHI, M., RONG, X., MOLL, S., KERS, J., FRANCO, C. and R. LORIA. 2007. Streptomyces turgidiscabies secretes a novel virulence protein, Nec1, which facilitates infection. Molecular Plant Microbe Interact, 20: 599-608. KHODAKARAMIAN, G.H., EINI, O. AND H. RAHIMIAN. 2003. Protein electrophoretic and fatty acid patterns of the strains of Streptomyces causing potato scab in Iran. Iranian Journal of Agricultural Science 34: 837-845. KRES, J.A., CAMERON, K.D., JOSHI, M.V., BUKHALID, R.A., MORELLO, J.E., WACH, M.J., GIBSON D.M. and R. LORIA. 2005. A large, mobile pathogenicity island confers plant pathogenicity on Streptomyces species. Molecular Microbiology 55: 1025–1033. LAMBERT, D. and R. Loria1989. Streptomyces scabies SP-Nov, nomrev. International Journal of Systematic and Evolutionary Microbiology, 39: 387-392. LAPAZ, M. I., HUGUET-TAPIA, J. C., VERDIER, E., LORIA, R. and M.J. PIANZZOLA. 2017. Genotypic and phenotypic characterization of Streptomyces species causing potato common scab in Uruguay. Plant Disease, 101: 1362-1372. LEIMINGER, J., FRANK, M., WENK, C., POSCHENRIEDER, G., KELLERMANN, A. and A. SCHWARZFISCHER.2013. Distribution and characterization of Streptomyces species causing potato common scab in Germany. Plant Pathology, 62: 611-623. LORIA, R., BUKHAID, R.A., CREATH, R.A., LEINER, R.H., OLIVIER, M. and J.C. STEFFENS. 1995. Differential production of thaxtomins by pathogenic Streptomyces species in vitro. Phytopathology 85:537–41 LORIA, R., BUKHAID, R.A., FRY, B. A. and R. R. King. 1997. Plant pathogenicity in the genus Streptomyces. Plant Disease, 81: 836-846. LORIA, R., COOMBS, J., YOSHIDA, M., KERS, J. A. and R. A. BUKHAID .2003. A paucity of bacterial root diseases: Streptomyces succeeds where others fail. Physiological and Molecular Plant Pathology, 62: 65-72. LORIA, R., KERS, J. and M. JOSHI. 2006. Evolution of plant pathogenicity in Streptomyces. Annual Review of Phytopathology, 44: 469-487. MIYAJOMA, K., TANAKA, F., TAKEUCHI, T. and S. KUNINAGA. 1998. Streptomyces turgidiscabies sp. nov. International Journal of Systematic Bacteriology, 48: 495-502. PANKOVA, L., SEDLAKOVA, V. and V. KREJZAR. 2012. The occurrence of plant pathogenic Streptomyces spp. in potato-growing regions in central Europe. American Journal of Potato Research, 89: 207-215. PARK, D., KIM, J., KWON, S., WILSON, C., YU, Y., HUR, J. and C. LIM. 2003. Streptomyces luridiscabiei sp. nov., Streptomyces puniciscabiei sp. nov and Streptomyces niveiscabiei sp. nov., which cause potato common scab disease in Korea. International Journal of Systematic and Evolutionary Microbiology, 53: 2049-2054. SARWAR, A., LATIF, Z. and C. CABALEIRO. 2018. First report of Streptomyces bottropensis causing potato common scab in Galicia, Spain. Plant Disease. doi:10.1094/PDIS-11-17-1803-PDN SCHAAD, N.W., JONES, J.B. and W. CHUN. 2001. Laboratory Guide for Identification of Plant Pathogenic Bacteria. 3nd ed. APS Press. USA. SCHEIBLE, W.W., FRY, B., KOCHEVENKO, A., SCHINDELASH, D., ZIMMERLI, L., SOMRVILLE, S., LORIA, R. and C. R. SOMERVILLE. 2003. An Arabidopsis mutant resistant to thaxtomin a, a cellulose synthesis inhibitor from Streptomyces species. The Plant Cell, 15: 1781-1794. SEIPKE, R. F. and R. LORIA. 2008. Streptomyces scabies 87-22 possesses a functional tomatinase. Journal of Bacteriology, 190: 7684-7692. SHIRLING, E.B. and D. GOTTLIEB. 1966. Methods for characterization of Streptomyces species. International Journal of Systematic Bacteriology 16, 313–40. SUSLOW, T.V., SCHROTH, M. and M. ISAKA. 1982. Application of a rapid method for gram differentiation of plant pathogenic and saprophytic bacteria without staining. Phytopathology 72: 917-918. TAGHAVI, S.M.and M.M. FAGHIHI. 2006. Distribution and identification of Streptomyces species, the causal agent of potato common scab in Fars province. Iranian Journal of Plant Pathology, 42: 85-116. TAMURA, K., PETERSON, D., STECHER, G., NEI, M. and S. KUMAR. 2011. MEGA5: Molecular evolutioary genetics analysis using maximum liklihood, evolutionary distance, and maximum parsimony methods. Molecular Biological Evolutionary, 28: 2731–2739. Tegg, R. S., Melian, L., Wilson, C. R. and S. SHABALA. 2005. Plant cell growth and ion flux responses to the Streptomycete phytotoxin thaxtomin a: calcium and hydrogen flux patterns revealed by the non-invasive MIFE technique. Plant Cell Physiology, 46: 638-648. THOMPSON, J.D., GIBSON, T.J., PLEWNIAK, F. and D.G. HIGGINS. 1997. The CLUSTALX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 24:4876–4882. WANNER, L. 2004. Field isolates of Streptomyces differ in pathogenicity and virulence on radish. Plant Disease, 88: 1363-1371. WANNER, L. 2007. High proportions of nonpathogenic Streptomyces are associated with common scab-resistant potato lines and less severe disease. Canadian Journal of Microbiology, 53: 1062-1075. WANNER, L. 2009. A patchwork of Streptomyces species isolated from potato common scab lesions in North America. American Journal of Potato Research, 86: 247-264. WANNER, L. A. 2006. A survey of genetic variation in Streptomyces isolates causing potato common scab in the United States. Phytopathology, 96: 1363-1371. WANNER, L. A., KIRK, W. W. and X. S. QU. 2013. Field efficacy of nonpathogenic Streptomyces species against potato common scab. Journal of Applied Microbiology, 116: 123-133. WEISBURG, W. G., BARNS, S. M., PELLETIER, D. and D. LANE. 1991. 16S Ribosomal DNA Amplification for Phylogenetic Study. Journal of Bacteriology, 173: 697-703. ZHANG, H., ZHOU, W., ZHUANG, Y., LIANG, X. and T. LIU. 2013. Draft genome sequence of Streptomyces bottropensis ATCC 25435, a bottromycin-producing actinomycete. Genome Announcement, 1(2):e00019-13. doi:10.1128/genomeA.00019-13. ZHOU, B., ZHANG, M.S. and X.K. MA. 2016. First Report of Streptomyces bottropensis causing potato common Scab in Hebei province, China. Plant Disease. doi:10.1094/PDIS-05-16-0671-PDN.
| ||
|
آمار تعداد مشاهده مقاله: 556 تعداد دریافت فایل اصل مقاله: 467 |
||