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اثر متابولیتها و آنتیاکسیدانها بر تحمل به یخزدگی ژنوتیپهای عدس در شرایط کنترل شده | ||
| نهال و بذر | ||
| دوره 36، شماره 3، آذر 1399، صفحه 273-299 اصل مقاله (1.24 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/sppi.2020.123483 | ||
| نویسندگان | ||
| جعفر نباتی* 1؛ احمد نظامی2؛ سیده محبوبه میرمیران3؛ سید سعید حجت1؛ محمد کافی4 | ||
| 1پژوهشکده علوم گیاهی، دانشگاه فردوسی مشهد، مشهد، ایران. | ||
| 2دانشکده کشاورزی و پژوهشکده علوم گیاهی، دانشگاه فردوسی مشهد، مشهد، ایران. | ||
| 3گروه کشاورزی دانشگاه پیام نور، تهران ایران. | ||
| 4گروه آگروتکنولوژی و پژوهشکده علوم گیاهی، دانشگاه فردوسی مشهد، مشهد، ایران. | ||
| چکیده | ||
| این مطالعه جهت بهگزینی تحمل به یخزدگی (13-، 15- و 18- درجهسانتیگراد) 40 ژنوتیپ عدس بهصورت فاکتوریل در قالب طرح کاملاً تصادفی تحت شرایط کنترلشده در سال 1397 در دانشگاه فردوسی مشهد انجام شد. بین ژنوتیپهای موردمطالعه ازنظر درصد بقا بعد از تنش یخزدگی و محتوای کلروفیل a، کاروتنوئیدها، آنتوسیانین، کربوهیدراتهای محلول، پرولین، مالوندیآلدئید، فنل، پروتئین، DPPH، آسکورباتپراکسیداز و پراکسیداز قبلاز تنش یخزدگی تفاوت معنیداری وجود داشت. مدل رگرسیونی نشان داد که کلروفیل b بیشترین تأثیر مثبت و پروتئین و مالوندیآلدئید بیشترین تأثیر منفی را بر درصدبقا داشتند. تجزیه خوشهای، ژنوتیپها را به پنج گروه تفکیک کرد. برای بیشتر صفات مورد مطالعه ژنوتیپهای گروه چهارم و پنجم نسبت به میانگین کل و ژنوتیپهای سایر گروهها برتری داشتند. در گروه چهارم محتوای آنتوسیانین، پروتئین، مالوندیآلدئید، فنل، پرولین، آسکورباتپراکسیداز و پراکسیداز نسبت به میانگین کل و سایر گروهها برتری داشت. درصدبقا، محتوای کلروفیلb، کاروتنوئیدها، کل رنگدانهها و DPPH گروه پنجم نسبت به سایر گروهها برتر بودند. تجزیه به مؤلفههای اصلی نشان داد که ژنوتیپهای MLC469، MLC458، MLC409، MLC74، MLC84،MLC169 ، MLC394، MLC95، MLC17، MLC163 و MLC303 ازنظر ظرفیت آنتیاکسیدانی و متابولیتها و ژنوتیپهای MLC70، MLC410، MLC47، MLC31، MLC91، MLC8، MLC286، MLC407 وMLC472 ، MLC61، MLC83 و MLC334 ازنظر ظرفیت رنگدانههای فتوسنتزی از وضعیت مطلوبی برخوردار هستند. بهطورکلی، بهنظر میرسد این شاخصها قبل از وقوع تنش سرما اهمیت زیادی در پیشبینی تأثیر سرما بر درصد بقا ژنوتیپهای عدس دارند. | ||
| کلیدواژهها | ||
| عدس؛ درصد بقا؛ پرولین؛ رنگدانه؛ آنتوسیانین | ||
| عنوان مقاله [English] | ||
| Effect of Metabolites and Antioxidants on Freezing Tolerance of Lentil Genotypes Under Controlled Conditions | ||
| نویسندگان [English] | ||
| J. Nabati1؛ A. Nezami2؛ S. M. Mirmiran3؛ S. S. Hojjat1؛ M. Kafi4 | ||
| 1Research Center for Plant Sciences, Ferdowsi University of Mashhad, Iran. | ||
| 2Faculty of Agriculture and Research Center for Plant Sciences, Ferdowsi University of Mashhad, Iran. | ||
| 3Department of Agronomy, Payam-e-Noor University, Tehran, Iran. | ||
| 4Agrotechnolgy Group and Research Center for Plant Sciences, Ferdowsi University of Mashhad, Mashhad, Iran. | ||
| چکیده [English] | ||
| To study freezing tolerance (-13, -15 and -18°C) of 40 lentil (Lens culinaris Medik.) genotypes, a factorial experiment in completely randomized design was carried out at the Research Center of Plant Science, Ferdowsi University of Mashhad, Mashhad, Iran, in 2017-2018. Significant differences observed between genotypes for survival percentage after freezing stress and chlorophyll a, carotenoids, anthocyanins, soluble carbohydrates, proline, malondialdehyde (MDA), phenol, protein, DPPH, ascorbate peroxidase and peroxidase before freezing stress. Stepwise regression analysis showed that chlorophyll b had the most positive effect and protein, and MDA had the most negative effect on survival percentage. Cluster analysis grouped genotypes into five clusters. For most of the studied traits, genotypes in clusters 4 and 5 were greater than average of all genotypes as well as genotypes in other clusters. In clusters 4, anthocyanins, protein, MDA, phenol, proline, ascorbate peroxidase and peroxidase contents were higher than other clusters. Survival percentage, chlorophyll b, carotenoids, total pigments, and DPPH of cluster 5 were higher than the other clusters. Principal component analysis showed that MLC469, MLC458, MLC409, MLC74, MLC84, MLC169, MLC394, MLC95, MLC17, MLC163 and MLC303 for antioxidant capacity and metabolites, and MLC70, MLC410, MLC47, MLC31, MLC91, MLC8, MLC286, MLC407, MLC472, MLC61, MLC83 and MLC334 for photosynthetic pigment capacity were more suitable than the other genotypes. It can be concluded that these attributes are very important in predicting, before cold stress, the effect of cold stress on survival percentage of lentil genotypes. | ||
| کلیدواژهها [English] | ||
| Lentil, pigment, proline, regression model, survival percentage, anthocyanin | ||
| مراجع | ||
|
Abe, N., Murata, T., and Hirota, A. 1998. Novel 1,1-diphenyl-2-picryhy- drazyl- radical scavengers, bisorbicillin and demethyltrichodimerol, from a fungus. Bioscience, Biotechnology, Biochemistry 62 (4): 61-662.
Ahmed, N. U., Park, J. I., Jung, H. J., Hur, Y., and Nou, I. S. 2015. Anthocyanin biosynthesis for cold and freezing stress tolerance and desirable color in Brassica rapa. Functional and Integrative Genomics 15 (4): 383-394.
Apostolova, P., Yordanova, R., and Popova, L. 2008. Response of antioxidative defense system to low temperature stress in two wheat cultivars. General and Applied Plant Physiology 34 (3-4): 281-294.
Atici, O., and Nalbantoglu, B. 2003. Antifreeze proteins in higher plants. Phytochemistry 64: 1187-1196.
Awasthi, R., Bhandari, K., and Nayyar, H. 2015. Temperature stress and redox homeostasis in agricultural crops. Frontiers in Environmental Science 3 (11): 1-24.
Bagheri, A. R. Nezami, A., and Hojjat, S. S. 2004. Evaluation of cold tolerance in lentil for fall planting in the highlands of Iran. Final report of research project. Ferdowsi University of Mashhad. (in Persian). 187 pp.
Balamurugan, S., Ann, J. S., Varghese, I. P., Murugan. Sh. M., Harish, M. Ch., Kumar, S. R., and Sathishkumar, R. 2018. Heterologous expression of Lolium perenne antifreeze protein confers chilling tolerance in tomato. Journal of Integrative Agriculture 17 (5): 1128–1136.
Barrios, A., Aparicio, T., J. Rodriguez, M., Perez de la Vega, M., and Caminero, C. 2016. Winter sowing of adapted lines as a potential yield increase strategy in lentil (Lens culinaris Medik.). Spanish Journal of Agricultural Research 14 (2): 1-8.
Bates, L. S., Waldren, R. P., and Teare, I. D. 1973. Rapid determination of free proline for water-stress studies. Plant and Soil 39 (1): 205-207.
Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72 (1-2): 248-254.
Caverzan, A., Casassola, A., and Patusssi Brammer, S. 2016. Antioxidant responses of wheat plants under stress. Genetics and Molecular Biology 39 (1): 1-6.
Chang, C. C., Yang, M. H., Wen, H. M., and Chern, J. C. 2002. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food and Drug Analysis 10 (3): 178-182.
Davey, M. W., Stals, E., Panis, B., Keulemans, J., and Swennen, R. I. 2005. High throughput of malondialdehyde in plant. Analytical Biochemistry 347: 201-207.
Dere, S., Gines, T., and Sivaci, R. 1998. Spectrophotometric determination of chlorophyll - a, b and total carotenoid contents of some algae species using different solvents. Turkish Journal of Botany 22 (1): 13-17.
Di Ferdinando, M., Brunetti, C., Fini, A., and Tattini, M. 2012. Flavonoids as antioxidants in Plants under abiotic stresses. pp. 159–179. In: Ahmad, P., Prasad, M. N. V. (Eds.) Abiotic stress responses in plants: metabolism, productivity and sustainability. Springer, New York.
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F. 1951. A colorimetric method for the determination of sugars. Nature 168 (4265): 167-167.
Duman, J. G., and Wisniewski, M. J. 2014. The use of antifreeze proteins for frost protection in sensitive crop plants. Environmental and Experimental Botany 106: 60–69.
FAO. 2017. Crop production statistics. Food and Agriculture Organization of United National, Rome, Italy (http://www.fao.org/faostat).
Gholami Rezvani, N., Nezami, A., Kafi, M., and Nabati, J. 2019. Evaluation of lentil (Lens culinaris) genotypes for autumn sowing in cold temperate regions under field conditions. Journal of Plant Production 11 (4): 142-147. (in Persian).
Gill, S. S., and Tuteja, N. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiology and Biochemistry 48: 909-930.
Gusta, L. V., and Wisniewski, M. 2013. Understanding plant cold hardiness: an opinion. Physiologia Planarum 147: 4–14.
Heath, R. L., and Packer, L. 1968. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics 125 (1): 189-198.
Hincha, D. K., and Zuther, E. 2020. Introduction: plant cold acclimation and winter survival. Methods in Molecular Biology 2156: 1-7.
Hojjat, S. S., and Galstyan, M. H. 2014. Study of economic-ecological results of cold resistance sort of the Lentil world collection under highlands of Islamic Republic of Iran. International Journal of Agriculture and Crop Science 7 (14): 1364-1370.
Hojjat, S. S., Bagheri, A. R., and Nezami, A. 2007. Evaluation of lentil germplasm for cold tolerance for fall planting in highlands of Iran. Journal of Agriculture Science 1: 19-31.
Homer, A., Sahin, M., and Kucukozdemir, U. 2016. Evaluation of pea (Pisum sativum L.) germplasm for winter hardiness in central Anatolia, Turkey, using field and controlled environment. Czech Journal of Genetics and Plant Breeding 52 (2): 55–63.
Janmohammadi, M., Enayati, V., and Sabaghnia, N. 2012. Impact of cold acclimation, de-acclimation and re-acclimation on carbohydrate content and antioxidant enzyme activities in spring and winter wheat. Icelandic Agricultural Sciences 25: 3-11.
Johnson, G. N. 2005. Cyclic electron transport in C3 plants: fact or artefact? Journal of Experimental Botany 56: 407–416.
Khan, M. I., Iqbal, N., Masood, A., Per, T. S., and Khan, N. A. 2013. Salicylic acid alleviates adverse effects of heat stress on photosynthesis through changes in proline production and ethylene formation. Plant Signaling and Behavior 8 (11), e26374, DOI: 10. 4161/psb26374.
Kim, S., Lee, S., and Lee, I. 2012. Alteration of phytotoxicity and oxidant stress potential by metal oxide nanoparticles in Cucumis sativus. Water, Air, and Soil Pollution 223: 2799-2806.
Ma, Y., Kuang, L., He, X., Bai, W., Ding, Y., Zhang, Z., Zhao, Y., and Chai, Z. 2010. Effects of rare earth oxide nanoparticles on root elongation of plants. Chemosphere 78: 273-279.
Miura, K., and Furumoto, T. 2013. Cold signaling and cold response in plants. International Journal of Molecular Science 14 (3): 5312–5337.
Muthuramalingam, M., Matros, A., Scheibe, R., Mock, H. P., and Dietz, K. J. 2013. The hydrogen peroxide-sensitive proteome of the chloroplast in vitro and in vivo. Frontiers in Plant Science 4: 54.
Oakley, C. G., Savage, L., Lotz, S., Larson, G. R., Thomashow, M. F., Kramer, D. M., and Schemske, D. W. 2018. Genetic basis of photosynthetic responses to cold in two locally adapted populations of Arabidopsis thaliana. Journal of Experimental Botany 69: 699–709.
Paredes, M., and Quiles, M. J., 2015. The effects of cold stress on photosynthesis in Hibiscus plants. PLoS One 10 (9): e0137472, DOI: 10.1371/journal. Pone. 0137472.
Potapovich, A. I., and Kostyuk, V. A. 2003. Comparative study of antioxidant properties and cytoprotective activity of flavonoids. Biochemistry (Moscow) 68 (5): 514–519.
Pradedova, E. V., Isheeva, O., and Salyaev, R. 2011. Classification of the antioxidant defense system as the ground for reasonable organization of experimental studies of the oxidative stress in plants. Russian Journal of Plant Physiology 58: 210-217.
Rahaie, M., Xue, G. P., and Schenk, P. M. 2013. The role of transcription factors in wheat under different abiotic stresses. pp. 367-385. In: Vahdati, K., and Leslie, C. (eds.) Abiotic stress - plant responses and applications in agriculture.
InTech, Rijeka, Croatia. Saibo, N. J. M., Lourenço, T., and Oliveira, M. M. 2009. Transcription factors and regulation of photosynthetic and related metabolism under environmental stresses. Annals of Botany 103: 609–623.
Schulz, E., Tohge, T., Zuther, E., R. Fernie, A., and K. Hincha, D. 2016. Flavonoids are determinants of freezing tolerance and cold acclimation in Arabidopsis thaliana. Scientific Reports: 1-10.
Sharma, P., Jha, A. B., Dubey, R. S., and Pessarakli, M. 2012. Reactive oxygen species, oxidative damage, and antioxidative defense mechanism in plants under stressful conditions. Journal of Botany: 1-26.
Singleton, V. L., and Rossi, J. A. 1965. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American Journal of Enology and Viticulture 16 (3): 144-158.
Soliman, M. H., Alayafi, A. A. M., El Kelish, A. A., and Abu-Elsaoud, A. 2018. Acetylsalicylic acid enhance tolerance of Phaseolus vulgaris L. to chilling stress, improving photosynthesis, antioxidants and expression of cold stress responsive genes. Botanical Studies 59: 6.
Sreenivasulu, N., Ramanjulu, S., Ramachandra-Kini, K., Prakash, H., Shekar-Shetty, H., Savithri, H., and Sudhakar, C. 1999. Total peroxidase activity and peroxidase isoforms as modified by salt stress in two cultivars of fox-tail millet with differential salt tolerance. Plant Science 141 (1): 1-9.
Thavarajah, D. 2017. Lentils (Lens culinaris L.): Linking whole foods for better human health. Nova Sciences Publishers. 229 pp.
Valizadeh Kamran, R., Toorchi, M., Moghadam, M., and Mohammadi, H. 2015. The effect of cold stress on H2O2 and MDA contents in barely genotypes. Journal of Biodiversity and Environmental Sciences 7 (3): 66-75.
Vitmas, P., Kosova, K., and Musilova, J. 2019. Relationship between dehydrin accumulation and winter survival in winter wheat and barley grown in the field. Frontiers in Plant Science 10: 7. DOI: 10.338q/fpls.2019.0007.
Wanger, G. J. 1979. Content and vacuole/ extra vacuole distribution of neutral sugars, free amino acids, and anthocyanin's in protoplast. Plant Physiology 64: 88-93.
Winfield, M. O., Lu, C, Wilson, L. D., Coghill, J. A., and Edwards, K. J. 2010. Plant responses to cold: transcriptome analysis of wheat. Plant Biotechnology Journal 8: 749-771.
Wisniewski, M., Glenn, D. M., and Fuller, M. P. 2002. Use of a hydrophobic particle film as a barrier to extrinsic ice nucleation in tomato plants. Journal of the American Society for Horticultural Science 127 (3): 358-364.
Yamaguchi, K., Mori, H., and Nishimura, M. 1995. A novel isoenzyme of ascorbate peroxidase localized on glyoxysomal and leaf peroxisomal membranes in pumpkin. Plant & Cell Physiology 36 (6): 1157-62.
Zhang, K. M., Yu, H. J., Shi, K., Zhou, Y. H., Yu, J. Q., and Xia, X. J. 2010. Photoprotective roles of anthocyanins in Begonia semperflorens. Plant Science 179 (3), 202-208.
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