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GENETIC DIVERSITY AMONG POPULATIONS OF THE TAMARIX SPECIES USING CDDP MOLECULAR MARKER | ||
| The Iranian Journal of Botany | ||
| دوره 26، شماره 2 - شماره پیاپی 52، اسفند 2020، صفحه 123-133 اصل مقاله (681.95 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22092/ijb.2020.342824.1281 | ||
| نویسندگان | ||
| Mohammad Ali Ghavampour1؛ Sayed abbas Mirjalili* 2؛ Mohammad Jafari3؛ Hossein Azarnivand4؛ Sayed Akbar Javadi5 | ||
| 1Rangeland Sciences, Natural Resources and Environment Faculty, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Associate Professor, Imam Khomeini Higher Education Center, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran | ||
| 3Faculty of Natural Resources, University of Tehran, Tehran, Iran | ||
| 4Faculty of Natural Resources, University of Tehran, Tehran, Iran. | ||
| 5Department of Rangeland Management, Natural Resources and Environment Faculty, Science and Research Branch, Islamic Azad University, Tehran, Iran. | ||
| چکیده | ||
| Tamarix L. is a problematic genus in taxonomy of Tamaricaceae due to the uncertainty of species number, distribution, and ecological conditions in Iran. In this study, we investigated the genetic diversity and taxonomic relationships of 34 individuals from 8 populations including 3 species of Tamarix in Isfahan province, Iran. Ten primers using CDDP molecular marker were used to survey the genetic diversity of the genus. One hundred and twenty-five bands were created from ten primers, of which 102 (80.16%) were polymorphic. Cluster analysis classified individuals into three distinct groups. High gene flow among Tamarix species using PCoA analysis showed high variability among the three Tamarix species, so that samples from different species were grouped together. Molecular analysis of variance showed that intra-population genetic diversity (90%) was greater than inter-population genetic diversity (10%). The highest mean Neiʼs genetic diversity (H) and Shannon diversity index (I) was observed in the Habib Abad population. Data analysis showed that morphological traits and DNA sequencing data in the genus Tamarix were not fully correlated, which could be justified by the large number of hybrids between species and the lack of genetic differentiation between the studied species. | ||
| کلیدواژه‌ها | ||
| Tamaricaceae؛ genetic diversity؛ CDDP marker؛ hybridization؛ Iran | ||
| عنوان مقاله [English] | ||
| تنوع ژنتیکی در میان جوامع گونه‌های گز با استفاده از نشانگر مولکولی CDDP | ||
| نویسندگان [English] | ||
| محمدعلی قوام‌پور1؛ سیدعباس میرجلیلی2؛ محمد جعفری3؛ حسین آذرنیوند4؛ سید اکبر جوادی5 | ||
| 1دانشجوی دکتری علوم مرتع، دانشکده منابع طبیعی و محیط زیست، شاخه علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 2مرکز آموزش عالی امام خمینی | ||
| 3دانشکده منابع طبیعی، دانشگاه تهران، تهران، ایران | ||
| 4دانشکده منابع طبیعی، دانشگاه تهران، تهران، ایران | ||
| 5گروه علوم مرتع، دانشکده منابع طبیعی و محیط زیست، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| چکیده [English] | ||
| سرده Tamarix به دلیل عدم قطعیت تعداد گونه‌ها، پراکنش و اهمیت اکولوژیکی در ایران، یکی از مهم‌ترین موضوعات در رده‌بندی تیره گز است. در این مطالعه، تنوع ژنتیکی و روابط خانوادگی 34 فرد از 8 جمعیت از 3 گونه Tamarix در استان اصفهان مورد بررسی قرار گرفت. ده آغازگر از نشانگر مولکولی CDDP برای بررسی تنوع ژنتیکی این سرده استفاده شد. 125 باند از ده آغازگر ایجاد شد، که از این تعداد 102 (16/80 درصد) چند شکلی بودند. آنالیز خوشه‌ای جمعیت‌ها را به سه گروه مجزا دسته‌بندی کرد. جریان بالای ژنی در میان گونه‌های Tamarix با استفاده از تجزیه و تحلیل PCoA تنوع زیادی در بین سه گونه Tamarix نشان داد، به طوری که نمونه‌های گونه‌های مختلف با هم گروه‌بندی شدند. تجزیه واریانس مولکولی نشان داد که تنوع ژنتیکی بین جمعیت (90٪) بیشتر از تنوع ژنتیکی درون جمعیت (10٪) است. بالاترین میانگین تنوع ژنتیکی نای (H) و شاخص تنوع شانون (I) در جمعیت حبیب‌آباد مشاهده شد. تجزیه و تحلیل داده‌ها نشان داد که صفات ریخت‌شناختی و داده‌های توالی DNA در سرده Tamarix کاملاً با هم ارتباط ندارند، که می‌تواند با وجود تعداد زیاد دورگه بین گونه‌ها و عدم تمایز ژنتیکی بین گونه‌های مورد مطالعه توجیه شود. | ||
| کلیدواژه‌ها [English] | ||
| Tamaricaceae, genetic diversity, CDDP marker, hybridization, Iran | ||
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