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Antidiabetic Screening, Activity-guided Isolation and Molecular Docking Studies of Flower Extracts of Pongamia pinnata (L.) Pierre | ||
| Journal of Medicinal plants and By-products | ||
| مقاله 11، دوره 10، شماره 1، تیر 2021، صفحه 85-92 اصل مقاله (508.67 K) | ||
| نوع مقاله: Research Article | ||
| شناسه دیجیتال (DOI): 10.22092/jmpb.2020.343368.1222 | ||
| نویسندگان | ||
| Farhat Saghir* 1؛ Khalid Hussain1؛ Muhammad Nawaz Tahir2؛ Syed Atif Raza1؛ Naureen Shehzadi1؛ Sadaf Iftikhar1؛ Ayisha Shaukat1؛ Surriya Naheed1؛ Sidra Siddique1 | ||
| 1Punjab University College of Pharmacy, University of the Punjab, Lahore, Pakistan | ||
| 2Department of Physics, University of Sargodha, Sargodha, Pakistan | ||
| چکیده | ||
| Pongamia pinnata (L.) Pierre (Family: Fabaceae) is a famous traditional medicinal plant, the flowers of which are used for treating diabetes, however, the active constituent(s) is yet unidentified. Therefore, the present study aimed to carry out antidiabetic activity-guided isolation of extracts of flowers. Hexane extract being the most active (EC50, 900 µg/mL) was fractionated by partitioning and the most active hexane fraction (EC50, 570 µg/mL) was subjected to column chromatography which gave three isomers of compound 1 (4-Methoxy-7-phenyl-5H-furo [3,2-g][1] benzopyran-5-one), a furanoflavonoid. All the isomeric forms have equal antidiabetic activity (83.24%) with EC50 at 300 µg/mL. The activity of the isolated compound was found to be higher as compared to standard drug acarbose (43.46%). Molecular docking studies of the compound indicated higher binding energy scores with antidiabetic targets as compared to the standard drug acarbose. The results of the present study indicate that the isolated compound may be developed into an antidiabetic drug. | ||
| کلیدواژهها | ||
| Pongamia pinnata (L.) Pierre؛ diabetes؛ Column chromatography؛ Single-crystal X-ray diffraction (XRD)؛ Molecular docking studies | ||
| مراجع | ||
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