| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شمارهها | 3,168 |
| تعداد مقالات | 35,197 |
| تعداد مشاهده مقاله | 50,944,034 |
| تعداد دریافت فایل اصل مقاله | 90,949,382 |
ارزیابی نشانگرهای مبتنی بر رتروترنسپوزونها در شناسایی مکانهای مرتبط با عملکرد و برخی صفات گیاهی در ژنوتیپهای کتان ایرانی (Linum usitatissimum L.) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 12، دوره 36، شماره 5 - شماره پیاپی 103، آذر و دی 1399، صفحه 851-866 اصل مقاله (929.51 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2020.342474.2758 | ||
| نویسندگان | ||
| حسین عباسی هولاسو* 1؛ فاطمه رحمانی2؛ مینا حسین زاده3؛ عبدالله حسن زاده قورت تپه4 | ||
| 1دانشجوی فرادکتری، گروه بهنژادی و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران | ||
| 2دانشیار، گروه زیستشناسی، دانشکده علوم، دانشگاه ارومیه، ارومیه، ایران | ||
| 3دانشآموخته کارشناسی ارشد، گروه زیستشناسی، دانشکده علوم، دانشگاه ارومیه، ارومیه، ایران | ||
| 4استادیار، بخش تحقیقات اصلاح و تهیه نهال و بذر، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان آذربایجانغربی، سازمان تحقیقات، آموزش و ترویج کشاورزی، ارومیه، ایران | ||
| چکیده | ||
| شناسه دیجیتال (DOR): 98.1000/1735-0905.1399.36.851.103.5.1575.41 با توجه به اینکه ارزش اقتصادی یک رقم به صفات مختلف آن بستگی دارد، بنابراین چگونگی اعمال انتخاب برای چندین صفت بهمنظور حصول حداکثر ارزش اقتصادی همواره مورد نظر بهنژادگران بوده است. ازاینرو اطلاع دقیق از رفتار و ارتباط ژنتیکی این صفات به اصلاح ژنوتیپها کمک خواهد نمود. در این تحقیق، بهمنظور شناسایی نشانگرهای مولکولی مرتبط با 21 صفت زراعی، مورفولوژیک و فیزیولوژیک در 45 ژنوتیپ مختلف کتان (Linum usitatissimum L.) براساس مدل خطی مخلوط، از 31 آغازگر مبتنی بر رتروترنسپوزون (12 آغازگر IRAP و 19 آغازگر REMAP) استفاده شد. مطالعه ساختار جمعیت به روش بیزین (Bayesian) نشان داد که دو زیرگروه احتمالی (2K=) در جمعیت مورد مطالعه وجود دارد. با استفاده از مدل خطی مخلوط در مجموع 29 مکان مرتبط با 21 صفت مورد مطالعه (p ≤0.01) شناسایی شد. صفات وزن صد دانه و عملکرد دانه بیشترین تعداد مکان پیوسته را به خود اختصاص دادند. وجود نشانگرهای مشترک در میان برخی صفات بررسی شده مانند ارتباط معنیدار نشانگر LTR1-UBC808-2 با صفات ارتفاع بوته و وزن صد دانه و نشانگر LTR1-UBC807-4 با صفات وزن ساقه اصلی و تعداد کپسول ساقه اصلی میتواند ناشی از آثار پلیوتروپی و یا پیوستگی نواحی ژنومی دخیل در این صفات باشد. باوجوداین، نتایج این مطالعه میتواند با گنجاندن تعداد بیشتری از نشانگرهایی که با صفات مهم اقتصادی مرتبط هستند، تقویت شود. | ||
| کلیدواژهها | ||
| ساختار جمعیت؛ عدم تعادل لینکاژی؛ عملکرد دانه؛ کتان (Linum usitatissimum L.)؛ مدل خطی مخلوط | ||
| عنوان مقاله [English] | ||
| Evaluation of the retrotransposon-based markers in identifying the loci associated with yield and some plant characteristics in Iranian flax (Linum usitatissimum L.) genotypes | ||
| نویسندگان [English] | ||
| H. Abbasi Holasou1؛ F. Rahmani2؛ M. Hosseinzadeh3؛ A. Hassanzadeh Ghorttapeh4 | ||
| 1Postdoctoral student, Department of Plant Breeding and Biotechnology, Faculty of Agricultural Sciences,Tabriz University, Tabriz, Iran | ||
| 2Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran | ||
| 3M.Sc. graduated, Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran | ||
| 4Seed and Plant Improvement Department, West Azerbaijan Agricultural and Natural Resources Research and Education Center Agricultural Research, Education and Extension Organization (AREEO), Urmia, Iran | ||
| چکیده [English] | ||
| Since the economic value of a cultivar depends on its different characteristics, thus procedure of selection for several traits to achieve maximum economic value has always been considered by plant breeders. Therefore, accurate knowledge of the behavior and genetic relationship of these traits will help breeders to improve plant genotypes. In this study, 31 retrotransposon-based primers (12 IRAP and 19 REMAP primers) were used to identify molecular markers associated with 21 agronomic, morphological, and physiological traits in 45 different flax (Linum usitatissimum L.) genotypes based on a mixed linear model (MLM). Analysis of population structure by Bayesian method revealed two possible subgroups (K=2) in the population studied. Using MLM, a total of 29 loci associated with the 21 traits studied were identified (p < /em><0.01). The maximum number of associated loci was identified for 100-seed weight and seed yield traits. The existence of common markers among some traits studied, such as the significant relationship between LTR1-UBC808-2 marker with plant height and 100-seed weight, and LTR1-UBC807-4 with main stem weight and the number of capsules per the main stem can be due to pleiotropic effects or linkage between genomic regions involved in these traits. However, findings of the present study can be further strengthened with the inclusion of more markers putatively associated with economically important traits. | ||
| کلیدواژهها [English] | ||
| Population structure, linkage disequilibrium, seed yield, Flax (Linum usitatissimum L.), mixed linear model | ||
| مراجع | ||
|
- Asgarinia, P., Cliutier, S., Duguid, S., Rashid, Kh., Mirlohi, A.F., Banik, M. and Saeidi, Gh., 2013. Mapping quantitative trait loci for powdery mildew resistance in flax (Linum usitatissimum L.). Crop science, 53: 2462-2472. - Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., Struhl, K., Albright, L.M., Coen, D.M. and Varki, A.A., 1995. Current protocols in molecular biology. John Wiley and Sons, New York, 750p. - Bradbury, P.J., Zhang, Z., Kroon, D.E., Casstevens, T.M., Ramdoss, Y. and Buckler, E.S., 2007. TASSEL: software for association mapping of complex traits in diverse samples. Bioinformatics, 23: 2633-2635. - Breseghello, F. and Sorrells, M.E., 2006. Association mapping of kernel size and milling quality in wheat (Triticum aestivum L.) cultivars. Genetics, 172: 1165-1177. - Choudhary, Sh.B., Sharma, H.K., Kumar, A.A., Chowdhaury, I., Maruthi, R.T. and Kak, A., 2017. Genetic diversity spectrum and marker trait association for agronomic traits in global accessions of Linum usitatissimum L. Industrial Crops and Products, 108: 604-615. - Cloutier, S., Ragupathy, R., Miranda, E., Radovanovic, N., Reimer, E., Walichnowski, A., Ward, K., Rowland, G., Duguid, S. and Banik, M., 2012. Integrated consensus genetic and physical maps of flax (Linum usitatissimum L.). Theoretical and Applied Genetics, 125: 1783-1795. - Earl, D.A., Louie, K.D., Bardeleben, C., Swift, C.C. and Jacobs, D.K., 2010. Rangewide microsatellite phylogeography of the endangered tidewater goby, Eucyclogobius newberryi (Teleostei: Gobiidae), a genetically subdivided coastal fish with limited marine dispersal. Conservation Genetics, 11: 103-114. - Evanno, G., Regnaut, S. and Goudet, J., 2005. Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Molecular Ecology, 14: 2611-2620. - Gupta, P.K., Rustgi, S. and Kulwal, P.L., 2005. Linkage disequilibrium and association studies in higher plants: Present status and future prospects. Plant Molecular Biology, 57: 461-485. - He, L., Xiao, J., Rashid, Kh.Y., Yao, Zh., Li, P., Jia, G., Wang, X., Cloutier, S. and You, F.M., 2019. Genome-wide association studies for pasmo resistance in flax (Linum usitatissimum L.). Frontiers in Plant Science, 9: 1982. - Hedrick, P.W., 1987. Gametic disequilibrium measures: proceed with caution. Genetics, 117: 331-341. - Hegary, S., Geleta, M., Bryngelsson, T., Asanaliev, A., Garkava-Gustavsson, L., Persson Hovmalm, H. and Ortiz, R., 2014. Genetic diversity analysis in Phaseolus vulgaris L. using morphological traits. Genetic Resources and Crop Evolution, 61: 555-566. - Jia, G. and Booker, H.M., 2018. Optimal models in the yield analysis of new flax cultivars. Canadian Journal of Plant Sciences, 98: 897-907. - Mackay, I.J., Bansep-Basler, P., Barber, T., Bentley, A.R., Cockram, J., Gosman, N., Greenland, A.J., Horsnell, R., Howells, R., Osullivan, D.M., Rose, G.A. and Howell, P.J., 2014. An eight-parent multiparent advanced generation inter-cross population for winter-sown wheat: creation, properties, and validation. G3-Genes Genomes Genetics, 4(9): 1603-1610. - Moose, S.P. and Mumm, R.H., 2008. Molecular plant breeding as the foundation for 21st century crop improvement. Plant Physiology, 147: 969-977. - Pritchard, J.K., Stephanes, M., Rosenberg, N.A. and Donnelly, P., 2000. Association mapping in structured populations. American Journal of Human Genetics, 67: 170-181. - Ragupathy, R., Rathinavelu, R. and Cloutier, S., 2011. Physical mapping and BAC-end sequence analysis provide initial insights into the flax (Linum usitatissimum L.) genome. BMC Genomics, 12(1): 217. - Rostok, N., Ramsay, L., MacKenzie, K., Cardle, L. and Bhat, P.R., 2006. Recent history of artificial outcrossing facilitates whole-genome association mapping in elite inbred crop varieties. Proceedings of the National Academy of Sciences of the United States of America, 103: 18656-18661. - Russo, M.A., Ficco, D.B.M., Marone, D., De Vita, P., Vallega, V., Pasam, R.K., Sharma, R., Malosetti, M., van Eeuwijk, F.A. and Haseneyer, G., 2012. Genome-wide association studies for agronomical traits in a worldwide spring barley collection. BMC Plant Biology, 12: 16. - Sharma, H.K., Sarkar, M., Choudhary, S.B., Kumar, A.A., Maruthi, R.T., Mitra, J. and Karmakar, P.G., 2016. Diversity analysis based on agro-morphological traits and microsatellite based markers in global germplasm collections of roselle (Hibiscus sabdariffa L.). Industrial Crops and Products, 89: 303-315. - Smykal, P., Bacova-Kerteszova, N., Kalendar, R., Corander, J., Schlman, A.H. and Pavelek, M., 2011. Genetic diversity of cultivated flax (Linum usitatissimum L.) germplasm assessed by retrotransposon-based markers. Theoretical and Applied Genetics, 122: 1385-1397. - Soto-Cerda, B.J., Diederichsen, A., Ragupathy, R. and Cloutier, S., 2013. Genetic characterization of a core collection of flax (Linum usitatissimum L.) suitable for association mapping studies and evidence of divergent selection between fiber and linseed types. BMC Plant Biology, 13: 78. - Soto-Cerda, B.J., Diederichsen, A., Duguid, S., Booker, H., Rowland, G. and Cloutier, S., 2014a. The potential of pale flax as a source of useful genetic variation for cultivated flax revealed through molecular diversity and association analysis. Molecular Breeding, 34(4): 2091-2107. - Soto-Cerda, B.J., Duguid, S., Booker, H., Rowland, G., Diederichsen, A. and Cloutier. S., 2014b. Association mapping of seed quality traits using the Canadian flax (Linum usitassimum L.) core collection. Theoretical and Applied Genetics, 127(4): 881-896. - Soto-Cerda, B.J., Duguid, S., Booker, H., Rowland, G., Diederichsen, A. and Cloutier, S., 2014c. Genomic regions underlying agronomic traits in linseed (Linum usitatissimum L.) as revealed by association mapping. Journal of Integrative Plant Biology, 56: 75-87. - Xie, D., Dai, Zh., Yang, Z., Tang, Q., Sun, J., Yang, X., Song, X., Lu, Y., Zhao, D., Zhang, L. and Su, J., 2018. Genomic variations and association study of agronomic traits in flax. BMC Genomics, 19: 512. - Yan, J., Shan, T., Warburton, M., Buckler, E., Mcmullen, M. and Crouch, J., 2009. Genetic characterization and linkage disequilibrium estimation of a global maize collection using SNP markers. PLoS One, 4: 8451. - You, F.M., Jia, G., Xiao, J., Duguid, S.D., Rashid, K.Y., Booker, H.M. and Cloutier, S., 2017. Genetic variability of 27 traits in a core collection of flax (Linum usitatissimum L.). Frontiers in Plant Science, 8: 1636. - Yu, J., Pressoir, G., Briggs, W.H., Vroh, B.I., Yamasaki, M. and Doebley, J.F., 2006. A unified mixed-model method for association mapping that accounts for multiple levels of relatedness. National Genetics, 38: 203-208. - Zhang, Q., Wu, C., Ren, F., Li, Y. and Zhang, C., 2012. Association analysis of important agronomical traits of maize inbred lines with SSRs. Australian Journal of Crop Science, 6(6): 1131-1138. - Zhu, C.M., Gore, E., Buckler, S. and Yu, J., 2008. Status and prospects of association mapping in plants. The Plant Genome, 1: 5-20. | ||
|
آمار تعداد مشاهده مقاله: 571 تعداد دریافت فایل اصل مقاله: 525 |
||