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ارزیابی نشانگرهای مبتنی بر رتروترنسپوزونها در شناسایی مکانهای مرتبط با عملکرد و برخی صفات گیاهی در ژنوتیپهای کتان ایرانی (Linum usitatissimum L.) | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 12، دوره 36، شماره 5 - شماره پیاپی 103، آذر و دی 1399، صفحه 851-866 اصل مقاله (929.51 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2020.342474.2758 | ||
| نویسندگان | ||
| حسین عباسی هولاسو* 1؛ فاطمه رحمانی2؛ مینا حسین زاده3؛ عبدالله حسن زاده قورت تپه4 | ||
| 1دانشجوی فرادکتری، گروه بهنژادی و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه تبریز، تبریز، ایران | ||
| 2دانشیار، گروه زیستشناسی، دانشکده علوم، دانشگاه ارومیه، ارومیه، ایران | ||
| 3دانشآموخته کارشناسی ارشد، گروه زیستشناسی، دانشکده علوم، دانشگاه ارومیه، ارومیه، ایران | ||
| 4استادیار، بخش تحقیقات اصلاح و تهیه نهال و بذر، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان آذربایجانغربی، سازمان تحقیقات، آموزش و ترویج کشاورزی، ارومیه، ایران | ||
| چکیده | ||
| شناسه دیجیتال (DOR): 98.1000/1735-0905.1399.36.851.103.5.1575.41 با توجه به اینکه ارزش اقتصادی یک رقم به صفات مختلف آن بستگی دارد، بنابراین چگونگی اعمال انتخاب برای چندین صفت بهمنظور حصول حداکثر ارزش اقتصادی همواره مورد نظر بهنژادگران بوده است. ازاینرو اطلاع دقیق از رفتار و ارتباط ژنتیکی این صفات به اصلاح ژنوتیپها کمک خواهد نمود. در این تحقیق، بهمنظور شناسایی نشانگرهای مولکولی مرتبط با 21 صفت زراعی، مورفولوژیک و فیزیولوژیک در 45 ژنوتیپ مختلف کتان (Linum usitatissimum L.) براساس مدل خطی مخلوط، از 31 آغازگر مبتنی بر رتروترنسپوزون (12 آغازگر IRAP و 19 آغازگر REMAP) استفاده شد. مطالعه ساختار جمعیت به روش بیزین (Bayesian) نشان داد که دو زیرگروه احتمالی (2K=) در جمعیت مورد مطالعه وجود دارد. با استفاده از مدل خطی مخلوط در مجموع 29 مکان مرتبط با 21 صفت مورد مطالعه (p ≤0.01) شناسایی شد. صفات وزن صد دانه و عملکرد دانه بیشترین تعداد مکان پیوسته را به خود اختصاص دادند. وجود نشانگرهای مشترک در میان برخی صفات بررسی شده مانند ارتباط معنیدار نشانگر LTR1-UBC808-2 با صفات ارتفاع بوته و وزن صد دانه و نشانگر LTR1-UBC807-4 با صفات وزن ساقه اصلی و تعداد کپسول ساقه اصلی میتواند ناشی از آثار پلیوتروپی و یا پیوستگی نواحی ژنومی دخیل در این صفات باشد. باوجوداین، نتایج این مطالعه میتواند با گنجاندن تعداد بیشتری از نشانگرهایی که با صفات مهم اقتصادی مرتبط هستند، تقویت شود. | ||
| کلیدواژهها | ||
| ساختار جمعیت؛ عدم تعادل لینکاژی؛ عملکرد دانه؛ کتان (Linum usitatissimum L.)؛ مدل خطی مخلوط | ||
| عنوان مقاله [English] | ||
| Evaluation of the retrotransposon-based markers in identifying the loci associated with yield and some plant characteristics in Iranian flax (Linum usitatissimum L.) genotypes | ||
| نویسندگان [English] | ||
| H. Abbasi Holasou1؛ F. Rahmani2؛ M. Hosseinzadeh3؛ A. Hassanzadeh Ghorttapeh4 | ||
| 1Postdoctoral student, Department of Plant Breeding and Biotechnology, Faculty of Agricultural Sciences,Tabriz University, Tabriz, Iran | ||
| 2Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran | ||
| 3M.Sc. graduated, Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran | ||
| 4Seed and Plant Improvement Department, West Azerbaijan Agricultural and Natural Resources Research and Education Center Agricultural Research, Education and Extension Organization (AREEO), Urmia, Iran | ||
| چکیده [English] | ||
| Since the economic value of a cultivar depends on its different characteristics, thus procedure of selection for several traits to achieve maximum economic value has always been considered by plant breeders. Therefore, accurate knowledge of the behavior and genetic relationship of these traits will help breeders to improve plant genotypes. In this study, 31 retrotransposon-based primers (12 IRAP and 19 REMAP primers) were used to identify molecular markers associated with 21 agronomic, morphological, and physiological traits in 45 different flax (Linum usitatissimum L.) genotypes based on a mixed linear model (MLM). Analysis of population structure by Bayesian method revealed two possible subgroups (K=2) in the population studied. Using MLM, a total of 29 loci associated with the 21 traits studied were identified (p < /em><0.01). The maximum number of associated loci was identified for 100-seed weight and seed yield traits. The existence of common markers among some traits studied, such as the significant relationship between LTR1-UBC808-2 marker with plant height and 100-seed weight, and LTR1-UBC807-4 with main stem weight and the number of capsules per the main stem can be due to pleiotropic effects or linkage between genomic regions involved in these traits. However, findings of the present study can be further strengthened with the inclusion of more markers putatively associated with economically important traits. | ||
| کلیدواژهها [English] | ||
| Population structure, linkage disequilibrium, seed yield, Flax (Linum usitatissimum L.), mixed linear model | ||
| مراجع | ||
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