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اثر نوع پیوند بر موفقیت ریزپیوندی درون شیشه ای برخی ارقام گیلاس | ||
| نهال و بذر | ||
| دوره 36، شماره 1، فروردین 1399، صفحه 129-135 اصل مقاله (950.96 K) | ||
| شناسه دیجیتال (DOI): 10.22092/sppi.2020.122900 | ||
| نویسندگان | ||
| محمد اسماعیل نداف* 1؛ ابراهیم گنجی مقدم2؛ غلامرضا ربیعی3؛ عبدالرحمن محمدخانی3 | ||
| 1دانشگاه شهرکرد دانشکده کشاورزی گروه باغبانی | ||
| 2بخش تحقیقات علوم زراعی و باغی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان خراسان رضوی، سازمان تحقیقات، آموزش و ترویج کشاورزی، مشهد، ایران. | ||
| 3گروه علوم باغبانی، دانشگاه شهرکرد، شهرکرد، ایران. | ||
| چکیده | ||
| This study was carried out to optimize the micrografting method in sweet cherry using factorial arrangements in completely randomized design with five replications. Experimental factors included; seven ceherry cultivars; Zard, Siah-e-Mashhad, Dovomras, Bing, Pishras, Takdaneh, Hajyousefi, and four grafting methods; wedge grafting without cover (G1C1), cleft grafting without cover (G2C1), wedge grafting with cover (G1C2), cleft grafting with cover (G2C2). Micrografting with micro-scion was performed and leafy primordia from sweet cherry cultivars grown on Rootstock of “Gisela 6” in vitro conditions. The micrografted plants were cultured on MS medium with 1 mgl-1 BAP, and after three weeks successful micrografted plants in the MS medium were transferred to a pot of perlite-peat moss (1: 1) for acclimation. There was significant difference among cultivars only for shoot length index. The main effect of grafting method and the interaction effect of cultivars × grafting method were significant on all indices, except on the micrografting success rates. In all sweet cherry cultivars, G2C2 grafting method had the highest successful grafting rate (42.9%), number of leaves (4.2 leaves), shoot growth (6.5 cm), and the shortest grafting time (2.2 days). Among the sweet cherry cultivars; cv. “Hajyousefi” had successful grafting rate (36.9%), cv. Siah-e Mashhad had the shortest grafting time (3 days), and cv. Zard had the highest number of leaves (3.3 leaves), and cv. Dovomras had the greatest shoot growth (5.4 cm). | ||
| کلیدواژهها | ||
| Sweet cherry؛ acclimation؛ leafy primordia؛ shoot tips؛ tissue culture | ||
| عنوان مقاله [English] | ||
| Effect of Grafting Method on In Vitro Micrografting Success of Some Sweet Cherry Cultivars | ||
| نویسندگان [English] | ||
| M.E. Naddaf1؛ E. Ganji Moghaddam2؛ Gh. Rabiei3؛ A. Mohammad Khani3 | ||
| 1department of horticulture, university of shahrekord | ||
| 2Field and Horticultural Crops Science Research Department, Khorasan Razavi Agricultural and Natural Resources Research and Education Center, Agricultural, Education and Extension Organization, Mashhad, Iran. | ||
| 3Horticultural Science Department, University of Shahr-e-Kord, Shahr-e-Kord, Iran. | ||
| چکیده [English] | ||
| This study was carried out to optimize the micrografting method in sweet cherry using factorial arrangements in completely randomized design with five replications. Experimental factors included; seven ceherry cultivars; Zard, Siah-e-Mashhad, Dovomras, Bing, Pishras, Takdaneh, Hajyousefi, and four grafting methods; wedge grafting without cover (G1C1), cleft grafting without cover (G2C1), wedge grafting with cover (G1C2), cleft grafting with cover (G2C2). Micrografting with micro-scion was performed and leafy primordia from sweet cherry cultivars grown on Rootstock of “Gisela 6” in vitro conditions. The micrografted plants were cultured on MS medium with 1 mgl-1 BAP, and after three weeks successful micrografted plants in the MS medium were transferred to a pot of perlite-peat moss (1: 1) for acclimation. There was significant difference among cultivars only for shoot length index. The main effect of grafting method and the interaction effect of cultivars × grafting method were significant on all indices, except on the micrografting success rates. In all sweet cherry cultivars, G2C2 grafting method had the highest successful grafting rate (42.9%), number of leaves (4.2 leaves), shoot growth (6.5 cm), and the shortest grafting time (2.2 days). Among the sweet cherry cultivars; cv. “Hajyousefi” had successful grafting rate (36.9%), cv. Siah-e Mashhad had the shortest grafting time (3 days), and cv. Zard had the highest number of leaves (3.3 leaves), and cv. Dovomras had the greatest shoot growth (5.4 cm). | ||
| کلیدواژهها [English] | ||
| Sweet cherry, acclimation, leafy primordia, shoot tips, tissue culture | ||
| مراجع | ||
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Amiri, M. 2006. In vitro technique to study the shoot tip grafting of sweet cherry (Prunus avium L.) var. Seeyahe Mashad. Journal of Food, Agriculture & Environment 41: 151-154.
Daneshvar Hossini, A., Ganji Moghadam, E., and Anahid, S. 2010. Effects of media cultures and plant growth regulators in micropropagation of ‘Gisela 6’ rootstock. Annals of Biology Research 1 (2): 135-141.
Deogratias, J., Lutz, A., and Dosba, F. 1986. In vitro micrografting of shoot tips from juvenile and adult Prunus avium L. and Prunus persica L. Batsch to produce virus-free plants. Acta Horticulturae 193: 139–45.
FAO. 2018. Http: //faostat.fao.org/faostat. Ganji Moghddam, E., and Bouzari, N. 2007. Practical and applied guide of sweet cherry; planting, husbandry and harvest. Agricultural Research, Education and Extensions Press. Mashhad, Iran. 344 pp. (in Persian).
Huang, S., and Millikan, D. F. 1980. In vitro micrografting of apple shoot tips. Horticulture Science 15: 741-743.
Hussain, G., Wani, M., Mir, M., Rather, Z., and Bhat, K. 2014. Micrografting for fruit crop improvement. African Journal of Biotechnology 13 (25): 2474-2483.
Martinez, J., Hugard, J., and Jonard, R. 1979. Different graft combinations with apices of peach, apricot and myrobolan realized in vitro. Academy of Sciences 288: 759-762.
Murashige, T., and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco cultures. Physiology of Plant 15: 437-497.
Navarro, L. 1988. Application of shoot-tip grafting in vitro to woody species. Acta Horticulturae 227: 43–55.
Navarro, L., Roistacher, C., and Murashige, T. 1975. Improvement of shoot-tip grafting in vitro for virus free citrus. Journal of the American Society for Horticultural Sciences 100: 471–479.
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