Effect of Grafting Method on In Vitro Micrografting Success of Some Sweet Cherry Cultivars | ||
| نهال و بذر | ||
| Volume 36, Issue 1, April 2020, Pages 129-135 PDF (950.96 K) | ||
| DOI: 10.22092/sppi.2020.122900 | ||
| Authors | ||
| M.E. Naddaf* 1; E. Ganji Moghaddam2; Gh. Rabiei3; A. Mohammad Khani4 | ||
| 1department of horticulture, university of shahrekord | ||
| 2Field and Horticultural Crops Science Research Department, Khorasan Razavi Agricultural and Natural Resources Research and Education Center, Agricultural, Education and Extension Organization, Mashhad, Iran. | ||
| 3Horticultural Science Department, University of Shahr-e-Kord, Shahr-e-Kord, Iran. | ||
| 4, Horticultural Science Department, University of Shahr-e-Kord, Shahr-e-Kord, Iran. | ||
| Abstract | ||
| This study was carried out to optimize the micrografting method in sweet cherry using factorial arrangements in completely randomized design with five replications. Experimental factors included; seven ceherry cultivars; Zard, Siah-e-Mashhad, Dovomras, Bing, Pishras, Takdaneh, Hajyousefi, and four grafting methods; wedge grafting without cover (G1C1), cleft grafting without cover (G2C1), wedge grafting with cover (G1C2), cleft grafting with cover (G2C2). Micrografting with micro-scion was performed and leafy primordia from sweet cherry cultivars grown on Rootstock of “Gisela 6” in vitro conditions. The micrografted plants were cultured on MS medium with 1 mgl-1 BAP, and after three weeks successful micrografted plants in the MS medium were transferred to a pot of perlite-peat moss (1: 1) for acclimation. There was significant difference among cultivars only for shoot length index. The main effect of grafting method and the interaction effect of cultivars × grafting method were significant on all indices, except on the micrografting success rates. In all sweet cherry cultivars, G2C2 grafting method had the highest successful grafting rate (42.9%), number of leaves (4.2 leaves), shoot growth (6.5 cm), and the shortest grafting time (2.2 days). Among the sweet cherry cultivars; cv. “Hajyousefi” had successful grafting rate (36.9%), cv. Siah-e Mashhad had the shortest grafting time (3 days), and cv. Zard had the highest number of leaves (3.3 leaves), and cv. Dovomras had the greatest shoot growth (5.4 cm). | ||
| Keywords | ||
| Sweet cherry; acclimation; leafy primordia; shoot tips; tissue culture | ||
| References | ||
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