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Ebnetorab, S.M.A., Ahari, Hamed, Kakoolaki, Shapour. (1399). Isolation, biochemical and molecular detection of Bacillus subtilis and Bacillus licheniformis from the digestive system of rainbow trout (Oncorhynchus mykiss) and its inhibitory effect on Aeromonas hydrophila. سامانه مدیریت نشریات علمی, 19(6), 2824-2845. doi: 10.22092/ijfs.2020.122770
S.M.A. Ebnetorab; Hamed Ahari; Shapour Kakoolaki. "Isolation, biochemical and molecular detection of Bacillus subtilis and Bacillus licheniformis from the digestive system of rainbow trout (Oncorhynchus mykiss) and its inhibitory effect on Aeromonas hydrophila". سامانه مدیریت نشریات علمی, 19, 6, 1399, 2824-2845. doi: 10.22092/ijfs.2020.122770
Ebnetorab, S.M.A., Ahari, Hamed, Kakoolaki, Shapour. (1399). 'Isolation, biochemical and molecular detection of Bacillus subtilis and Bacillus licheniformis from the digestive system of rainbow trout (Oncorhynchus mykiss) and its inhibitory effect on Aeromonas hydrophila', سامانه مدیریت نشریات علمی, 19(6), pp. 2824-2845. doi: 10.22092/ijfs.2020.122770
Ebnetorab, S.M.A., Ahari, Hamed, Kakoolaki, Shapour. Isolation, biochemical and molecular detection of Bacillus subtilis and Bacillus licheniformis from the digestive system of rainbow trout (Oncorhynchus mykiss) and its inhibitory effect on Aeromonas hydrophila. سامانه مدیریت نشریات علمی, 1399; 19(6): 2824-2845. doi: 10.22092/ijfs.2020.122770

Isolation, biochemical and molecular detection of Bacillus subtilis and Bacillus licheniformis from the digestive system of rainbow trout (Oncorhynchus mykiss) and its inhibitory effect on Aeromonas hydrophila

Iranian Journal of Fisheries Sciences
دوره 19، شماره 6، بهمن 2020، صفحه 2824-2845    اصل مقاله (874.27 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22092/ijfs.2020.122770
نویسندگان
S.M.A. Ebnetorab* 1؛ Hamed Ahari2؛ Shapour Kakoolaki* 3
1Department of Food Science and Technology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2IAU
3IFSRI
چکیده
Our study was focused on characterization of dominantBacillus bacteria isolated from the digestive tract of Oncorhynchus mykiss. After 8 weeks, the fish fed with probiotic-free diet were dissected under strictly controlled conditions. Fish intestines were eviscerated and its content diluted to 10−4 using Ringer solution. Two isolated bacteria were Gram-positive, catalase-positive, and oxidase-negative, based on the biochemical tests. A mixed of the two strains was the most efficient isolates determined inO. mykiss. Molecular characterization using PCR-Ribotyping was carried out using sequencing the 16S rRNA gene of both strains, which isolated two bacteria, Bacillus subtilis Ahari.H1 and B. licheniformis Ahari. H2. The isolated bacteria could tolerate 0.3% bile salt and non-hemolytic in nature. They also showed resistance to pH at 2.5 and 4 for acid test and 2.3 and 7 for gastric juice, pepsin and trypsin. It is concluded that the efficiency of the isolated probiotics can be candidates as probiotic in fish culture. It was unveiled that these strains can successfully diminish Aeromonas hydrophila pathogenicity in rainbow trout.
کلیدواژه‌ها
Rainbow trout؛ Probiotic؛ Bacillus subtilis Ahari.H1؛ Bacillus licheniformis Ahari.H2؛ PCR
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