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بررسی اثر روشهای مختلف ضدعفونی برای پینه زایی و شاخساره زایی موفق گز شاهی (Tamarix aphylla) | ||
| تحقیقات ژنتیک و اصلاح گیاهان مرتعی و جنگلی ایران | ||
| دوره 28، شماره 1 - شماره پیاپی 55، شهریور 1399، صفحه 115-131 اصل مقاله (1.06 M) | ||
| نوع مقاله: مقاله علمی - پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijrfpbgr.2020.126989.1347 | ||
| نویسندگان | ||
| پروین کریمی1؛ مینا تقی زاده* 2؛ موسی سلگی3؛ علی خدیوی3 | ||
| 1دانشآموخته کارشناسی ارشد، گروه علوم و مهندسی باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه اراک | ||
| 2استادیار، گروه علوم و مهندسی باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه اراک | ||
| 3دانشیار، گروه علوم و مهندسی باغبانی، دانشکده کشاورزی و منابع طبیعی، دانشگاه اراک | ||
| چکیده | ||
| گز شاهی (Tamarix aphylla) بهعنوان بادشکن، سایهبان و گیاه زینتی در باغها و فضای سبز کاربرد دارد. پدیده قهوهای شدن، عامل بازدارنده مهمی در مراحل مختلف ازجمله گندزدایی و باززایی گز شاهی میباشد. برای بهینه کردن شرایط گندزدایی و کنترل شرایط قهوهای شدن ریزنمونهها، آزمایشهایی با مواد گندزدا و ائوجینول برای گندزدایی ریزنمونههای ساقه فصل جاری و یکساله اجرا گردید. در ادامه القای پینه در محیط کشت دارای دو نوع اکسین 2,4-D و NAA بررسی شد. در آزمایش سوم نیز بهینهسازی محیط القای شاخساره در ریزنمونههای فصل جاری و یکساله بررسی گردید. بهترین تیمار گندزدایی برای کنترل آلودگیهای قارچی، باکتریایی و ترشح فنل از ریزنمونههای فصل جاری، استفاده از ترکیب 60 ثانیه اتانول همراه 10 دقیقه گندزدایی هیپوکلریت سدیم 20 درصد بود. محلولپاشی پایه مادری با قارچکش تیوفانات متیل نیز توانست به مقدار تقریبا 50 درصد آلودگی قارچی را نسبت به شاهد کاهش دهد. کاربرد 01/0 درصد ائوجینول در محیط کشت به صورت تزریقی سبب کاهش آلودگیها و ترشح فنل از ریزنمونه شد. غلظت یک میلیگرم در لیتر 2,4-D بیشترین میزان زندهمانی و القای پینه را در کمترین زمان ایجاد نمود. القای شاخساره در محیط کشت دارای دو میلیگرم در لیتر BA و 5/0 میلیگرم در لیتر IBA در ریزنمونههای حاصل از شاخه سال جاری و یکساله انجام شد. بنابراین استفاده از مواد گیاهی جوانتر، محلولپاشی پایه مادری با قارچکش، استفاده از ائوجنول 01/0 درصد در محیط کشت میتواند با کاهش پدیده قهوهای شدن و کنترل مناسب آلودگیها، ریزازدیادی درختچه گز شاهی را با استفاده از تنظیم کنندههای رشد مناسب فراهم کند. | ||
| کلیدواژهها | ||
| گز شاهی (Tamarix aphylla)؛ قهوهای شدن؛ گندزدایی؛ ائوجینول؛ قارچکش؛ ریزازدیادی | ||
| عنوان مقاله [English] | ||
| Investigation of the effect of various sterilization procedures for successful callus and shoot proliferation of Tamarix aphylla | ||
| نویسندگان [English] | ||
| Parvin Karimi1؛ Mina Taghizadeh2؛ Mousa Solgi3؛ Ali Khadivi3 | ||
| 1M.Sc. Graduated, Department of Horticultural Science and Engineering, Faculty of Agricultural and Natural Resources, Arak University, Arak, I.R. Iran | ||
| 2Assist. Prof., Department of Horticultural Science and Engineering, Faculty of Agricultural and Natural Resources, Arak University, Arak, I.R. Iran. | ||
| 3Assoc. Prof. Department of Horticultural Science and Engineering, Faculty of Agricultural and Natural Resources, Arak University, Arak, I.R. Iran. | ||
| چکیده [English] | ||
| Tamarix aphylla is used as a windbreaker, shade tree and ornamental plant in gardens and urban landscape. The browning phenomenon is an important inhibitory factor in various stages such as disinfection and regeneration of Tamarix aphylla. To optimize the disinfection conditions and control the browning process of the explants, experiments were carried out with disinfectants and eugenol compound to disinfect the current and annual stem explants. Then, callus induction was studied in a medium complemented by two types of auxins (2,4-D and NAA). In the third experiment, the optimization of shoot induction medium was investigated on current and one year old shoots explants. The best disinfection treatment to control fungal and bacterial contamination and phenol secretion from the current explants was obtained using a 60-second ethanol application combined with 10 minutes of 20% sodium hypochlorite disinfection. Spraying of donor plants with thiophanate methyl fungicide was also able to reduce fungal contamination by almost 50% compared to the control. Application of 0.01% eugenol in the medium by injection reduced the contamination and phenol secretion from the explants. The highest survival and callus induction was acquired at the concentration of 1 mgL-1 2, 4-D, in the shortest time. Shoot induction in the explants of current and one year old shoots was obtained in the culture medium containing 2 mgL-1 BA and 0.5 mgL-1 IBA In conclusion, the application of younger plant materials, spraying of donor plant with fungicides and the use of 0.01% eugenol in the medium can reduce the browning phenomenon and proper control of contamination and finally could provide micropropagation of Tamarix aphylla shrubs using appropriate growth regulators | ||
| کلیدواژهها [English] | ||
| Tamarix aphylla, Browning, Disinfection, Eugenol, Fungicide, Micropropagation | ||
| مراجع | ||
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