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بررسی تاثیر محیط کشت ، دما و pH بر عملکرد ریزجلبک اسپیرولینا پلاتنسیس در فتوبیوراکتور عمودی | ||
| تحقیقات سامانه‌ها و مکانیزاسیون کشاورزی | ||
| دوره 21، شماره 76، دی 1399، صفحه 99-116 اصل مقاله (2.15 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/erams.2020.343130.1358 | ||
| نویسندگان | ||
| محمد حسین افشار بخش1؛ احمد محمدی* 2؛ حمید مشهدی3؛ فهیمه محمود نیا4 | ||
| 1دانشجوی دکتری گروه مکانیک بیوسیستم . دانشکده کشاورزی. واحد اراک. دانشگاه آزاد اسلامی اراک | ||
| 2استادیار گروه مکانیزاسیون کشاورزی ، دانشکده کشاورزی، واحد اراک، دانشگاه آزاد اسلامی ، اراک، ایران | ||
| 3استادیار گروه مکانیک بیوسیستم. دانشکده کشاورزی، واحد اراک، دانشگاه آزاد اسلامی ، اراک، ایران | ||
| 4استادیار گروه میکرو بیولوژِی . دانشگاه فرهنگیان (شهید بهشتی) .قم، ایران | ||
| چکیده | ||
| این تحقیق به بررسی تاثیر دما، pH و نوع محیط کشت بر فاکتورهای موثر در تولید ریزجلبک اسپیرولینا پلاتنسیس پرداخته است. آزمایشی با استفاده از طرح فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار در فتوبیوراکتور عمودی به اجرا درآمد. عامل اول یعنی محیط کشت ریزجلبک در سه سطح: محیط کشت والن، جردن و BBM، عامل دوم دما در دو سطح: 25 و 30 درجه سلسیوس و عامل سوم میزان اسیدیته در دو سطح: 5/0±8 و 5/0±10 بود. میزان شدت نور 5000 لوکس، مدت نوردهی 14 ساعت و هوادهی از طریق پمپ هوا با اختلاط میزان دی‌اکسید کربن 15 درصد برای هر 36 نمونه به صورت یکسان اعمال گردید. پارامترهای اندازه‌گیری شامل: میزان تراکم سلولی، زیست‌توده، درصد چربی، میزان کلروفیل a و کارتنوئید است. نتایج بررسیها نشان داد که فاکتورهای تراکم سلولی، میزان زیست‌توده، درصد لیپید، میزان کلروفیل a و کارتنوئید در بین محیطهای کشت والن، جردن و BBM در سطح 1 درصد و بین pH و دماهای انتخابی نیز تفاوت معنیداری وجود دارد. نتایج مقایسۀ میانگین دادهها نشان داد بیشترین میزان تراکم سلولی، میزان کلروفیل a، میزان کارتنوئید مربوط به تیمار M1T1P1 (محیط کشت والن با دمای 5 و 5/0±8=pH) و کمترین میزان مربوط به تیمار M2T2P2 (محیط کشت BBM با دمای 30 و 5/0±10 pH=) است. در خصوص زیست‌توده، بیشترین میزان در تیمار M3T1P1 (محیط کشت جردن با دمای 25 و 5/0±8pH=) به دست آمد. تحقیق حاضر نشان داد از بین شرایط مختلف تولید، مناسب‌ترین آن برای تولید اسپیرولینا پلاتنسیس محیط کشت والن با دمای 25 درجه سلسیوس با 5/0±8 pH= است. | ||
| کلیدواژه‌ها | ||
| زیست توده؛ کلروفیل؛ کارتنوئید؛ لیپید؛ میکروجلبک | ||
| عنوان مقاله [English] | ||
| Effect of Culture Medium, Temperature and pH on the Performance of Spirulina Platensis Microalgae in Vertical Photobioreactor | ||
| نویسندگان [English] | ||
| Mohammad Hoseyn Afsharbakhsh1؛ ahmad mohammadi2؛ HAMID MASHHADI3؛ Fahimeh Mahmod nia4 | ||
| 1Ph. D. student, Department of Biosystem Mechanics, Faculty of Agriculture, Islamic Azad University, Arak Branch, Arak | ||
| 2Assistant Professor, Department of Agricultural Mechanization, Faculty of Agriculture, Islamic Azad University, Arak Branch | ||
| 3Assistant Professor, Department of Mechanical Biosystems. Faculty of Agriculture. Islamic Azad University, Arak Branch. Arak | ||
| 4Assistant Professor, Microbiology Group. Farhangian University (Shahid Beheshti). Qom | ||
| چکیده [English] | ||
| The current study quantified the effects of culture media and environmental factors (temperature and pH) on factors in the production of microalgae Spirulina platensis by photobioreactor. The culture of microalgae was done separately in Walne, BBM, and Jordan media. Temperature, pH, and light intensity (5000 lux for all stages and 16 hours per day) were applied in each culture medium separately. Cell density, biomass, lipid percentage, chlorophyll a, and carotenoids were measured for all treatments. The differences in parameter's effects were assessed by SPSS software. The results showed a significant difference at 1% in cell density, biomass, chlorophyll, carotenoid, and lipid content in the Walne, BBM, and Jordan culture medium at pH (8±0.5&10±0.5) and temperature (25 & 30 °C), respectively. Average data revealed that the highest cell density, amount of chlorophyll a, the number of carotenoids were found in M1T1P1 treatment (Walne culture medium at 25 ◦C and PH=8±0.5), and the lowest amount was found in M2T2P2 treatment (BBM culture medium at 30 °C and PH10±0.5). In terms of biomass, the highest levels were obtained in M3T1P1 treatment (Jordan culture medium at 25 °C and PH=8±0.5). Conclusions showed that Walne medium culture at pH=9 and temp 25 °C was suitable for microalgae of Spirulina. But from the point of the cost of culture medium, Jordan culture medium seemed to be more economical. The results showed that there was a significant difference at 1% in cell density, biomass, chlorophyll, carotenoid, and lipid content in the walne, BBM, and Jordan culture medium at pH (8±0.5&10±0.5) and temperature (25 & 30 °C), respectively. According to the results of comparing the average data, the highest cell density, chlorophyll a, the number of carotenoids related to M1T1P1 treatment (walne culture medium with 25 ◦C and PH8±0.5), and the lowest amount related to M2T2P2 treatment (BBM culture medium with 30 °C and PH10±0.5 (In terms of biomass, the highest levels were obtained with M3T1P1 treatment (Jordan culture medium at 25 °C and PH8±0.5). The present study, with a scientific criterion, showed that Walne medium culture with PH9 and temp 25 °C was suitable for microalgae of Spirulina. But due to the cost of culture medium, Jordan culture medium is more economical. | ||
| کلیدواژه‌ها [English] | ||
| : Biomass, Carotenoid, Chlorophyl, Lipid, Microalgae | ||
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