| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شماره‌ها | 3,086 |
| تعداد مقالات | 34,442 |
| تعداد مشاهده مقاله | 48,905,737 |
| تعداد دریافت فایل اصل مقاله | 79,346,562 |
تأثیر منابع کربن و نیتروژن بر رشد و اسپورزایی Bacillus thuringiensis var. tenebrionis | ||
| مهار زیستی در گیاه‌پزشکی | ||
| دوره 7، شماره 2 - شماره پیاپی 14، اسفند 1398، صفحه 49-62 اصل مقاله (390.18 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2020.122492 | ||
| نویسندگان | ||
| فاطمه صابری1؛ رسول مرزبان* 2؛ مهدی ارجمند3؛ فرشید پژوم شریعتی4؛ امید توکلی5 | ||
| 1گروه مهندسی شیمی، دانشگاه آزاد اسلامی واحد علوم تحقیقات، تهران، ایران | ||
| 2مؤسسه تحقیقات گیاه پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، تهران، ایران | ||
| 3گروه مهندسی شیمی، دانشگاه آزاد اسلامی واحد تهران جنوب، تهران، ایران | ||
| 4گروه مهندسی شیمی، دانشگاه آزاد اسلامی واحد علوم تحقیقات، تهران، ایران | ||
| 5دانشکده مهندسی شیمی، پردیس دانشکده‌های فنی، دانشگاه تهران، تهران، ایران | ||
| چکیده | ||
| برای بومی‌سازی دانش تولید tenebrionisvar. Bacillus thuringiensis کنترل‌کننده آفات سخت بالپوش، اولین گام پس از جداسازی باکتری، بهینه‌سازی محیط تولید می‌باشد که در پروژه حاضر مورد مطالعه قرار گرفت. در این مطالعه ازB. thuringiensis var. tenebrionis–BN1 (Btt–BN1) به عنوان یک آفت‌کش بیولوژیک برای کنترل سوسک برگخوار نارون استفاده شد. هدف از بهینه کردن محیط کشت مطلوب، افزایش راندمان تولید آفت‌کش بیولوژیکB. thuringiensis با ارزش افزوده بالا می‌باشد که در این محیط کشت از ضایعات کشاورزی و صنایع غذایی (ملاس نیشکر، جو دوسر، سبوس گندم و شربت ذرت) به عنوان مواد اولیه ارزان قیمت استفاده شده است. برای بهینه‌سازی پارامترهای محیط کشت از طراحی آزمایش به روش پاسخ سطح RSM)) و با رویکرد طراحی مرکب مرکزی استفاده شد. متغیرهای مستقل شامل مقدار منبع کربن و مقدار منبع نیتروژن در 3 سطح غلظت (6/0 تا 1 درصد ملاس نیشکر، 3/0 تا 9/0 درصد جو دوسر، 3/0 تا 9/0 درصد سبوس گندم و 1 تا 5 درصد شربت ذرت) با پاسخ واحدهای تشکیل‌دهنده پرگنه (CFU) و درصد تلفات برگخوار نارون مورد بررسی قرار گرفت. نتایج بدست آمده در شرایط عملیاتی pH = 7 و دمای 30 درجه سلسیوس پس از 72 ساعت نشان داد که حداکثر میزان CFU برای حداقل غلظت جو دوسر (3/0%)، حداقل غلظت سبوس گندم (3/0%)، حداکثر غلظت ملاس نیشکر (1 %) و حداقل غلظت شربت ذرت (1 %) معادل spores.ml−18.3611 بدست آمد. همچنین نتایج زیست‌سنجی Btt–BN1 روی برگخوار نارون نشان داد دو غلظت 5/0 و 1 درصد به ترتیب 7/67% و 80% تلفات ایجاد می‌کند. | ||
| کلیدواژه‌ها | ||
| Bacillus thuringiensis var. tenebrionis؛ برگخوار نارون؛ راندمان تولید؛ بستر تولید؛ شرایط رشد | ||
| عنوان مقاله [English] | ||
| Influence of carbon and nitrogen sources on the growth and sporulation of Bacillus thuringiensis var. tenebrionis | ||
| نویسندگان [English] | ||
| Fatemeh Saberi1؛ Rasoul Marzban2؛ Mehdi Ardjmand3؛ Farshid Pajoum Shariati4؛ Omid Tavakoli5 | ||
| 1Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 2Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran | ||
| 3Department of Chemical Engineering, Tehran South Branch, Islamic Azad University, Tehran, Iran | ||
| 4Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran | ||
| 5School of Chemical Engineering, College of Engineering, University of Tehran, Tehran 14176, Iran | ||
| چکیده [English] | ||
| For knowledge localization of Bacillus thuringiensis (Bt) production as biological pest control, optimization of culture medium is necessary. In this study, Bacillus thuringiensis var. tenebrionis–BN1 (Btt–BN1) was used as a biological pesticide to control elm leaf beetles. The aim of optimizing the desire culture medium is to increase the production efficiency of Bt biopesticide using agricultural and food wastes as cheap raw materials. To optimize the parameters of the culture medium, experimental design of response surface methodology (RSM) was applied with a central composite design (CCD) approach. The parameters including the amount of carbon (sugar cane molasses, oats and wheat bran sources) and the amount of nitrogen (corn syrup source) at three levels (0.6 to 1 percent of sugar cane molasses, 0.3 to 0.9 percent of oat, 0.3 to 0.9 percent of wheat bran and 1 to 5 percent of corn syrup) were investigated with colony forming unit (CFU) response. The results obtained under operating conditions of pH = 7, temperature of 30oC after 72 hours. The results showed that the maximum CFU for the minimum concentration of oats (0.3%), the minimum concentration of wheat bran (0.3%), the maximum concentration of sugar cane molasses (1%) and the minimum concentration of corn syrup (1%) were equivalent to 8.36 11 spores.ml–1. In addition, the results of Btt–BN1 bioassay survey on elm leaf beetle showed that two concentrations of 0.5 and 1.0 percent cause 67.7% and 80.0% of mortality, respectively. | ||
| کلیدواژه‌ها [English] | ||
| Bacillus thuringiensis var. tenebrionis, elm leaf beetle, production efficiency, production substrate, growth conditions | ||
| مراجع | ||
|
Anderson, R.K.I. & Jayaraman, K. 2003. Influence of carbon and nitrogen sources on the growth and sporulation of Bacillus thuringiensis var Galleriae for biopesticide production. Chemical and Biochemical Engineering, 17 (3): 225–231. Azmi, N.U., Ghafar, N.S.A., Yin, C.J., Yakubu, S., Adli, A.A. & Aziz, N.A.A. 2015. Toxicity of Bacillus thuringiensis biopesticide produced in shrimp pond sludge as alternative culture medium against Bactrocera dorsalis (Hendel). Acta Biology of Malaysia, 4(5): 16–20. Ballardo, C., Barrena, R., Artola, A. & Sánchez, A. 2017. A novel strategy for producing compost with enhanced biopesticide properties through solid–state fermentation of biowaste and inoculation with Bacillus thuringiensis. Waste Management, 70: 53–58. Baur, R., Binder, S. & Benz, G. 1991. Nonglandular leaf trichomes as short–term inducible defence of the grey alder, Alnus incana (L.), against the chrysomelid beetle, Agelastica alni L. Oecologie, 87(2): 219–226. Brar, S.K., Verma, M., Tyagi, R.D., Valéro, J.R. & Surampalli, R.Y. 2005. Starch industry wastewater–based stable Bacillus thuringiensis liquid formulations. Journal of Economic Entomology, 98: 1890–1898. Cannon, R.J. 1993. Prospects and progress for Bacillus thuringiensis–based pesticides. Pest Management Science, 37(4): 331–335. Chandrashekhar Devidas, P., Hemant Pandit, B. & Satish Vitthalrao, P. 2014. Evaluation of different culture media for improvement in bioinsecticides production by indigenous Bacillus thuringiensis and their application against larvae of aedes aegypti. Scientific World Journal, 1: 1–7. Elleuch, J., Jaoua, S., Ginibre, C., Chandre, F., Tounsi, S. & Zghal, R.Z. 2016. Toxin stability improvement and toxicity increase against dipteran and lepidopteran larvae of Bacillus thuringiensis crystal protein Cry2Aa. Pest Management Science, 72(12): 2240–2246. Ferro, D.N., Yuan, Q.C., Slocombe, A. & Tuttle, A.F. 1993. Residual activity of insecticides under field conditions for controlling the Colorado potato beetle (Coleoptera: Chrysomelidae). Journal of Economic Entomology, 86: 511–516. Gnepe, J.R., Tyagi, R.D., Brar, S.K., Valéro, J.R. & Surampalli, R.Y. 2014. Corrosion and stability study of Bacillus thuringiensis var. kurstaki starch industry wastewater–derived biopesticide formulation. Journal of Environment Science Health B, 49)11): 889–896. Gorret, N., Rosli, S.K., Oppenheim, S.F., Willis, L.B., Lessard, P.A. & Rha, C.K. 2004. Bioreactor culture of oil palm (Elaeis guineensis) and effects of nitrogen source, inoculum size, and conditioned medium on biomass production. Journal of Biotechnology, 108: 253–63. Icgen, Y., Icgen, B. & Ozcengiz G. 2002. Regulation of crystal protein biosynthesis by Bacillus thuringiensis: II. Effects of carbon and nitrogen sources, Research of Microbiology, 153: 605–609. Kalantari M., Marzban R., Magollifard Z., & Abbasipour H. 2013. Study of virulence and molecular characteristics of some Bacillus thuringiensis isolates on cotton bollworm and diamondback moth. Biocontrol in Plant Protection, 2 (2): 17–26. Khanh Dang Vu, R.D., Tyagi, J.R. & Valéro, R.Y. 2009. Impact of different pH control agents on biopesticidal activity of Bacillus thuringiensis during the fermentation of starch industry wastewater. Bioprocess and Biosystem Engineering, 32 (4): 511–519. Langenbruch, G.A., Krieg, A., Huger, A.M. & Schnetter, W. 1985. Erste feldversuche zur bekämpfung der larven des kartoffelkäfers (Leptinotarsa decemlineata) mit Bacillus thuringiensis subsp. tenebrionis. Mededelingen Faculteit Landbouwkunde Rijksuniversiteit Gent, 50: 441–449. Marrone, P.G. 1999. Microbial pesticides and natural products as alternatives. Outlook on Agriculture, 28(3): 149–154. Marzban R. 2012. Investigation on the suitable isolate and medium for production of Bacillus thuringiensis. Journal of Biopesticides, 5: 144–147. Marzban R., Saberi F., & Shirazi M.M. 2016. Microfiltration and ultrafiltration of Bacillus thuringiensis fermentation broth: Membrane performance and spore-crystal recovery approaches. Brazilian Journal of Chemical Engineering, 33 (4): 783–791. Mazid, S. & Kalita, J.C. 2011. A review on the use of biopesticides in insect pest management. International Journal of Science Advanced Technology, 1: 169–178. Melo, A.L.d.A., Soccol, V.T. & Soccol, C.R. 2016. Bacillus thuringiensis: mechanism of action, resistance, and new applications: a review. Critical Review of Biotechnology, 36(2): 317–326. Oberemok, V.V., Laikova, K.V., Gninenko, Y.I., Zaitsev, A.S., Nyadar, P.M. & Adeyemi T.A. 2015. A short history of insecticides. Journal of Plant Protection Research, 55: 221–226. Osman, G., Already, R., Assaeedi, A., Organji, S., El–Ghareeb, D., Abulreesh, H. & Althubiani, A. 2015. Bioinsecticide Bacillus thuringiensis a comprehensive review. Egyptian Journal of Biological Pest Control, 25(1): 271–288. Ozkan, M., Dilek, F.B., Yetis, U. & Ozcengiz, G. 2003. Nutritional and cultural parameters influencing antidipteran delta–endotoxin production. Research of Microbiology, 154 (1): 49–53. Rao, Y.K., Tsay, K.J., Wu, W.S. & Tzeng, Y.M. 2007. Medium optimization of carbon and nitrogen sources for the production of spores from Bacillus amyloliquefaciens B128 using response surface methodology. Process Biochemistry, 42: 535–541. Rodriguez, P., Cerda, A., Font, X., Sanchez, A. & Artola, A. 2019. Valorisation of biowaste digestate through solid–state fermentation to produce biopesticides from Bacillus thuringiensis. Waste Management, 93: 63–71. Saberi F., Marzban R. & Ardjmand M. 2014. Optimization of Bacillus thuringiensis production process in lab Fermenter. Biological Control of Pests & Plant Diseases, 3(2): 165–172. Sarrafzadeh, M.H. 2014. Nutritional requirements of Bacillus thuringiensis during different phases of growth, sporulation and germination evaluated by Plackett–Burman method. Iranian Journal of Chemistry and Chemical Engineering, 31(4): 131–136 Sayed, A.M. & Behle, R.W. 2017. Evaluating a dual microbial agent biopesticide with Bacillus thuringiensis var. kurstaki and Beauveria bassiana blastospores. Biocontrol Science and Technology, 27(4): 461–474. Schnepf, H.E., Crickmore, N., Van Rie, J., Lereclus, D., Baum, J., Feitelson, J., Zigler, D.R. & Dean, D.H. 1998. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiology and Molecular Biology Reviews, 62 (3): 775–806. Sezen, K., Muratoglu, H., Nalcacioglu, R., Mert, D., Demirbag, Z. & Kati, H. 2008. Highly pathogenic Bacillus thuringiensis subsp. tenebrionis from European shot–hole borer, Xyleborus dispar (Coleoptera: Scolytidae). New Zealand Journal of Crop Horticulture Science, 36:v77–84. Suchy, J. 1988. Note on the biology of the Chrysomelid, Agelastica alni (L.) and the predator Hister helluo Truqui. Zpravy Muzei Zapadoces Kraj, 93: 36–37. Thurston, G.S. 1998. Biological Control of Elm Leaf Beetle. Journal of Arboriculture, 24(3): 154–159. Tokcaer, Z., Bayraktar, E., Mehmetoglu, U., Ozcengiz, G. & Alaeddinoglu, N.G. 2006. Response surface optimization of antidipteran delta–endotoxin production by Bacillus thuringiensis subsp. israelensis HD 500, Process Biochemistry, 41: 350–355. Urban, J. 1999. Results of the study of biology and harmfulness of alder leaf beetle (Agelastica alni L.) (Chrysomelidae: Coleoptera). Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 47: 47–71. Yezza, A., Tyagi, R.D., Valero, J.R. & Surampalli, R.Y. 2004. Scale–up of biopesticide production processes using wastewater sludge as a raw material. Journal of Indian Microbiology and Biotechnology, 31: 545–552. Zouari, N., Achour, O. & Jaoua, S. 2002. Production of delta–endotoxin by Bacillus thuringiensis subsp kurstaki and overcoming of catabolite repression by using highly concentrated gruel and fishmeal media in 2– and 20–dm 3 fermenters. Journal of Chemical Technology and Biotechnology, 77: 877–882. | ||
|
آمار تعداد مشاهده مقاله: 698 تعداد دریافت فایل اصل مقاله: 585 |
||