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شناسایی مولکولی نژادهای قارچ Fusarium oxysporum f. sp. ciceris، عامل پژمردگی نخود در استانهای غربی و شمال غربی ایران | ||
| آفات و بیماری های گیاهی | ||
| مقاله 2، دوره 88، شماره 1 - شماره پیاپی 110، شهریور 1399، صفحه 11-22 اصل مقاله (667.5 K) | ||
| نوع مقاله: بیماریشناسی گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2020.126209.1281 | ||
| نویسندگان | ||
| جعفر شکری1؛ محمد جوان نیکخواه* 2؛ سعید رضایی3؛ حمیدرضا زمانی زاده4؛ خشنود نورالهی5 | ||
| 1گروه گیاهپزشکی، دانشکده علوم کشاورزی و صنایع غذایی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
| 2استاد/گروه گیاهپزشکی، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران، کرج | ||
| 3دانشگاه آزاد واحد علوم و تحقیقات | ||
| 4هیئت علمی دانشگاه آزاد واحد علوم وتحقیقات تهران | ||
| 5گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه ایلام | ||
| چکیده | ||
| استانهای غربی و شمال غربی ایران دارای بیشترین پتانسیل برای کشت نخود در ایران هستند. پژمردگی آوندی که توسط قارچ Fusarium oxysporum f. sp. ciceris (FOC) ایجاد میشود یکی از بیماریهای مهم نخود است که درکشتهای نخود این استانها انتشار دارد. این پژوهش باهدف شناسایی نژادهای بیماریزای قارچ FOC در استانهای غربی و شمال غربی کشور انجام شد. نمونهبرداری از سه کانون آلودگی در استانهای فوق انجام و پس از جداسازی، شناسایی جدایه های قارچ با بهکارگیری آغازگرهای اختصاصی FOC به روش PCR انجام شد. از بین ۱۱۶ جدایه شناساییشده، ۹۴ جدایه متعلق به سه کانون، برای شناسایی نژاد انتخاب شدند. پنج نژاد R0، R1B/C، R1A، R6 و R5 به ترتیب بالاترین فراوانی در مناطق مورد مطالعه را داشتند. حضور پنج نژاد در استانهای غربی و شمال غربی ایران از بین هشت نژاد شناساییشده این قارچ در دنیا، نشاندهنده تنوع ژنتیکی بالای قارچ در کشور است. پراکنش نژادهای این قارچ مستقل از شرایط محیطی است. | ||
| کلیدواژهها | ||
| آغازگر؛ پراکنش؛ پی سی آر؛ تنوع ژنتیکی | ||
| عنوان مقاله [English] | ||
| Molecular identification of the races of Fusarium oxysporum f. sp. ciceris, causal agent of chickpea wilt in western and north western provinces of Iran | ||
| نویسندگان [English] | ||
| Jafar Shokri1؛ Mohammad Javan-Nikkhah2؛ Saeid Rezaei3؛ Hamid reza zamanizadeh4؛ Khoshnoud Nourollahi5 | ||
| 1Department of Plant Pathology, College of Agriculture Sciences and Food Industries, Science and Research Branch, Islamic Azad university, Tehran, Iran | ||
| 2Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran | ||
| 3Azad University of Tehran | ||
| 4Azad University of Tehran | ||
| 5Ilam University | ||
| چکیده [English] | ||
| Western and north western provinces of Iran have the highest potential for chickpea cultivation in Iran. Vascular wilt caused by Fusarium oxysporum f. sp. ciceris (FOC) is one of the most important diseases of this crop which distributed in chickpea cultivation areas of these provinces. The purpose of this study was to identify the pathogenic races of FOC in the western and north western provinces. Sampling was conducted on three infected locations. After isolation of the fungal isolates, identification was performed using FOC specific primers by PCR method. Out of 116 isolates identified, 94 isolates belonging to three locations were selected to recognize the fungal races. Five races including R0, R1B/C, R1A, R6 and R5 showed the highest abundance in the studied areas, respectively. Presence of five races from the eight identified races of FOC in the world in western and north western provinces of Iran indicates the high genetic diversity of this fungus in the country and distribution of the races of this fungus is independent of environmental factors. | ||
| کلیدواژهها [English] | ||
| Distribution, genetic diversity, polymerase chain reaction (PCR), primer | ||
| مراجع | ||
|
ANONYMOUS., 2016. Agricultural Statistics. Ministry of Agriculture, Planning and Economic Deputy, Office of Statistics and Information Technology, 514 pp. ARVAIO-ORTIZ, M., M. ESQUEDA, E. ACEDO-FELIX, A. SANCHEZ and A. GUTIERREZ, 2011. Morphological variability and races of Fusarium oxysporum f. sp. ciceris associated with chickpea (Cicer arietinum) crops. American Journal of Agricultural and Biological Sciences, 6: 114-121. BAGHERI, A., A. NEZAMI, A. GANEALI and M. PARSA, 1997. The chickpea. Publications University of Mashhad, 444 pp. BAYRAKTAR, H., and F. S. DOLAR, 2012. Pathogenic variability of Fusarium oxysporum f. sp. ciceris isolates from chickpea in Turkey. Pakistan Journal of Botany, 44: 821-823. BHATTI, M. A. and J. M. KRAFT, 1992. Effects of inoculums density and temperature on root rot and wilt of chickpea. Plant Disease, 76: 809–810. CASTELLANOS, J. Z., J. X. UVALLA and A. AGUILAR, 2000. Soil and Water Analysis Interpretation Manual. 2 Edn, INCAPA, Mexico, 226pp. DI PIETRO, A., F. I. GARCIA-MACEIRA, E. MEGLECZ and M. I. RONCERO, 2001. A MAP kinase of the vascular wilt fungus Fusarium oxysporum is essential for root penetration and pathogenesis. Molecular Microbiology, 39: 1140-1142. DOYLE, J. J. and J. L. DOYLE, 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, 19: 11–15. DUBEY, S. C., K. PRIYANKA, V. SINGH and B. SINGH, 2012. Race Profiling and Molecular Diversity Analysis of Fusarium oxysporum f.sp. ciceris Causing Wilt in Chickpea. Phytopathology, 160: 576-587. ELMAHI MOHAMED, O., A. HAMWIEH, S. AHMED and N. ELMAHI MOHAMED, 2015. Genetic Variability of Fusarium oxysporum f.sp. ciceris Population Affecting Chickpea in the Sudan. Phytopathology, 163: 941-946. FISHER, N. L., W. BURGESS, T. A. TOUSSOUN and P. E. NELSON, 1982. Carnation leaves as a substrate and for preserving cultures of Fusarium species. Phytopathology, 72: 151-153. GUPTA, S., D. CHAKRABORTI, R. K. RANGI, D. BASU and S. DAS, 2009. A molecular insight into the early events of chickpea (Cicer arietinum) and Fusarium oxysporum f. sp. ciceris (Race 1) interaction through cDNA-AFLP analysis. Phytopathology, 99: 1245-1267. HALILA, H. M. and R. STRANGE, 1996. Identification of the causal agent of wilt of chickpea in Tunisia as Fusarium oxysporum f. sp. ciceris race 0. Phytopathologia Mediterranea, 35: 67–74. HAWARE, M. P., and Y. L. NENE, 1982. Races of Fusarium oxysporum f. sp. ciceri. Plant Disease, 66: 809–810. JIMENEZ-DIAZ, R. M., A. R. ALCALAl-JIMENEZ, A. HERVAS and J. L. TRAPERO-CASAS, 1993. Pathogenic variability and host resistance in the Fusarium oxysporum f. sp. ciceris/Cicer arietinum pathosystem. In: E. Arseniuk and T. Goral (Eds). Proceedings of the 3rd European Seminar on Fusarium Mycotoxins, Taxonomy, Pathogenicity and Host Resistance (pp. 87–94). Plant Breeding and Acclimatization Institute, Radzikóv, Poland. JIMENEZ-GASCO, M. M. and R. M. JIMENEZ-DIAZ2003. Development of a specific polymerase chain reaction-based assay for the identification of Fusarium oxysporum f. sp. ciceris and its pathogenic races 0, 1A, 5, and 6. Phytopathology, 93: 200–209. JIMENEZ-GASCO, M. M., E. PEREZ-ARTES and R. M. JIMENEZ-DIAZ, 2001. Identification of pathogenic races 0, 1B/C, 5, and 6 of Fusarium oxysporum f. sp. ciceris with random amplified polymorphic DNA (RAPD). European Journal of Plant Pathology, 107: 237–248. JIMENEZ-GASCO, M. M., M. G. MILGROOM, and R. R. M. JIMENEZ-DIAZ, 2003.Stepwise evolution of races in Fusarium oxysporum f. sp. ciceris inferred from fingerprinting with repetitive DNA sequences. Phytopathology, 94: 228–235. LESLIE, J. F. and B. A. SUMMERELLA, 2006. The Fusarium Laboratory Manual. Blackwell Publishing Professional‚ USA, 388pp. LANDA, B. B., O. V. MAVRODI, K. L. SCHROEDER, R. ALLENDE-MOLA and D. M. WELLER, 2006. Enrichment and genotypic diversity of phlD-containing fluorescent Pseudomonas spp. in two soils after a century of wheat and flax monoculture. Microbiology Ecology, 55: 351-368. NELSON, P. E., T. A. TOUSSOUNA and W. F. O. Marasas, 1983. Fusarium species: An illustrated manual for identification. The Pennsylvania State University Press University Park 193 pp. NENE, Y. L., 1988. Multiple-disease resistance in grain legumes. Annual Review of Phytopathology‚ 26: 203-217. NOGALES-MONCADA, A. M., R. M. JIMENEZ-DIAZ and E. PEREZ-ARTES, 2009. Vegetative compatibility groups in Fusarium oxysporum f. sp. ciceris and F. oxysporum non-pathogenic to chickpea. Journal of Phytopathology, 157: 729–735. NOUROLLAHI, K., A. ALIARAN and H. YONESSI, 2016. Genetic diversity of F. oxysporiumf. sp. ciceris isolates causal agent of wilt chickpea in Kermanshah province using microsatellite markers. Modern Genetics Journal, 11:605-615. PADILLA-VALENZUELA, I., R. I. VALENZUELA-VALENZUELA, C. M. ARMENTA-CASTRO, R. A. SALINAS-PEREZ and E. SANCHEZ-SANCHEZ, 2008.Agronomic behavior of chickpea genotypes in delayed sowing at the Mayo Valley, Sonora, Mexico. Revista Fitotecnia Mexicana, 31: 43-49. SHEHABU, M., S. AHMED and K. SAKHUJA, 2008. Pathogenic variability in Ethiopian isolates of Fusarium oxysporum f. sp. ciceris and reaction of chickpea improved varieties to the isolates. International Journal of Pest Management, 54: 143–149. SIVARAMAKRISHNAN, S., S. KANNAN and S.D. SINGH, 2002. Genetic variability of Fusarium wilt pathogen isolates of chickpea (Cicer arietinum L.) assessed by molecular markers.Mycopathologia, 155: 171-178. TRAPERO-CASAS, A. and R. M. JIMENEZ-DIAZ, 1985. Fungal wilt and root rot diseases of chickpea in southern Spain. Phytopathology, 75: 1146–1151. VUJANOVIC, V., C. HAMEL, E. YERGEAU and M. ST-ARNAUD, 2006.Biodiversity and biogeography of Fusarium species from northeastern North American asparagus fields based on microbiological and molecular approaches. Microbial Ecology, 51:242-255. YOUNESI, H., 2004. Identification of the physiologic races of Fusarium oxysporum f. sp. ciceris in some west provinces of Iran. Summary of Articles in the 16th Iranian Plant Protection Congress, Tabriz. | ||
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