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بررسی چندشکلی ژنهای DNMTs در گاو سیستانی با استفاده از روش PCR-SSCP | ||
| علوم دامی | ||
| دوره 33، شماره 126، خرداد 1399، صفحه 73-82 اصل مقاله (1.69 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2019.124714.1847 | ||
| نویسندگان | ||
| سجاد شهدادی ساردو1؛ مهدی وفای واله* 2؛ غلامرضا داشاب3؛ محمد رکوعی3 | ||
| 1دانش آموخته کارشناسی ارشد علوم دامی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران | ||
| 2دانشیار گروه علوم دامی، دانشکده کشاوزی، دانشگاه زابل، زابل، ایران | ||
| 3دانشیار گروه علوم دامی، دانشکده کشاورزی، دانشگاه زابل، زابل، ایران | ||
| چکیده | ||
| نشان داده شده است که بروز هر گونه تغییر در عملکرد ژنهای DNMTs اثرات شگرفی بر فرآیند تکوین جنین و نیز وزن تولد در پستانداران دارد، لذا هدف از انجام این تحقیق بررسی چندشکلی ژنهای خانواده DNA متیل ترانسفراز و ارتباط آن‌ها با وزن تولد در گاو سیستانی بود. خون‌گیری به طور تصادفی از 60 رأس گاو سیستانی به عمل آمد. استخراج DNA با استفاده از روش فنول‌کلروفرم انجام شد. نواحی کاندیدا برای وجود جهش شامل قطعه‌های 114 جفت بازی واقع در اگزون 33 ژن DNMT1، قطعه 176 جفت بازی واقع در اینترون 4 ژن DNMT3a و قطعه 207 جفت بازی واقع در اینترون 3 ژن DNMT3b توسط واکنش زنجیره‌ای پلی‌مراز تکثیر شدند. به منظور بررسی وجود چندشکلی در ژنهای هدف از روش PCR-SSCP به‌وسیله‌ی ژل پلی آکریل آمید و رنگ‌آمیزی نیترات نقره استفاده گردید. ارزیابی نتایج دلالت بر عدم وجود تنوع الگوهای باندی در تمام نمونه‌های مورد بررسی داشت. بر اساس نتایج این مطالعه به نظر می‌رسد که عدم مشاهده تنوع ژنتیکی در نواحی مورد بررسی احتمالاً دلالت بر انتخاب بر علیه وقوع جهش های خاص در جمعیت مورد نظر دارد. در مجموع براساس نتایج این مطالعه ارزیابی تنوع ژنتیکی در نواحی مورد بررسی، جهت شناسائی مارکرهای موثر در برنامه‌های اصلاحی صفات تولیدی در گاو سیستانی فاقد کارائی می باشد | ||
| کلیدواژه‌ها | ||
| ژنهای DNA متیل ترانسفراز؛ چند شکلی؛ گاو سیستانی؛ PCR-SSCP | ||
| عنوان مقاله [English] | ||
| Analysis of polymorphism in DNMTS genes in Sistani cattle Using PCR-SSCP Method | ||
| نویسندگان [English] | ||
| Sajjad Shahdadi Sardo1؛ Mehdi Vafaye Valleh2؛ Gholam Reza Dashab3؛ Mohammad Rokouei3 | ||
| 1M.Sc, Department of Animal Science, Faculty of Agriculture, University of Zabol, Zabol, Iran | ||
| 2Department of Animal Science, Faculty of Agriculture, University of Zabol, Zabol, Iran | ||
| 3Associate Professor of University of Zabol | ||
| چکیده [English] | ||
| It has been shown that the occurrence of any changes in the function of DNMTs genes has a significant effect on both the embryo development and birth weight in mammals; therefore, the aim of the present study was to investigate the presence of DNA polymorphisms in the members of DNA methyl transferase superfamily and its relationship with birth weight in Sistani cattle’s. Blood sampling was done randomly from 60 Sistani cows. DNA extraction was performed using phenol chloroform method. Candidate region for the presence of functional polymorphism within the DNMTs gene including the 114-bp fragment of the exon 33 of the DNMT1 gene, the 176-bp fragment of the intron 4 of the DNMT3a gene, and the 207-bp fragment in intron 3 of the DNMT3b gene were amplified by polymerase chain reaction. PCR-SSCP followed by polyacrylamide gels silver stain analysis was done to evaluate the presence of candidate mutations in target gens. The result analysis of all analyzed region did not show any mutations in the investigated DNMTs genes. Based on the results of this study, it seems that the lack of observation of genetic diversity in the studied regions could be related to the evolutionary selection against occurrence of specific mutations in the analyzed population. In general, based on the results of this study, analysis of genetic diversity in the studied genomic region may not be effective in identifying effective markers for breeding programs in Sistani cows. | ||
| کلیدواژه‌ها [English] | ||
| DNA Methyltransferases Genes, polymorphism, Sistani cattle, PCR-SSCP | ||
| مراجع | ||
|
بیرجندی، م. ر. (1376). بررسی وضعیت پرورش و تعیین توان تولید شیر و خصوصیات شیرواری گاو سیستانی در منطقه سیستان. چکیده طرح‌های تحقیقاتی وزارت جهاد سازندگی (جلد دوم). ص 368-370. وزارت جهاد سازندگی. Biniszkiewicz, D., Gribnau, J., Ramsahoye, B., Gaudet, F., Eggan, K., Humpherys, D. et al. (2002). Dnmt1 overexpression causes genomic hypermethylation, loss of imprinting, and embryonic lethality. Molecular and Cellular Biology, 22(7): 2124-2135. Boligon, A. A., Mercadante, M. E. Z., Forni, S., Lobo, R. B. and Albuquerque, L. G. D. (2010). Covariance functions for body weight from birth to maturity in Nellore cows. Journal of Animal Science, 88(3): 849-859. Cheng, P., Chen, H., Zhang, R.P., Liu, S.R. and Zhou-Cun, A. (2014). Polymorphism in DNMT1 may modify the susceptibility to oligospermia. Reproductive biomedicine online, 28: 644-649. Chew, B. P., Maier, L. C., Hillers, J. K. and Hodgson, A. S. (1981). Relationship Between Calf Birth Weight and Dam's Subsequent 200-and 305-Day Yields of Milk, Fat, and Total Solids in Holsteins1. Journal of Dairy Science, 64(12): 2401-2408. Dawson, W. M., Phillips, R. W. and Black, W. H. (1947). Birth weight as a criterion of selection in beef cattle. Journal of Animal Science, 6(3): 247-257. Delaval, K. and Feil, R. (2004). Epigenetic regulation of mammalian genomic imprinting. Current opinion in Genetics and Development, 14(2): 188-195. Denis, H., Ndlovu, M. N. and Fuks, F. (2011). Regulation of mammalian DNA methyltransferases: a route to new mechanisms. EMBO Reports, 12: 647–656. Eden, S., Constancia, M., Hashimshony, T., Dean, W., Goldstein, B., Johnson, A. C. et al. (2001). An upstream repressor element plays a role in Igf2 imprinting. The EMBO Journal, 20(13): 3518-3525. Fan, H., Liu, D. S., Zhang, S. H., Hu, J. B., Zhang, F. and Zhao Z. J. (2008). DNMT3B 579 G>T promoter polymorphism and risk of esophagus carcinoma in Chinese. World Journal of Gastroenterology, 14: 2230. Farias, L. C., De Carvalho Fraga, C. A., De Oliveira, M. V. M., Silva, T. F., Marques-Silva, L., Moreira, P. R. et al. (2010). Effect of age on the association between p16CDKN2A methylation and DNMT3B polymorphism in head and neck carcinoma and patient survival. International Journal of Oncology, 37(1): 167-176. Golding, M. C., Williamson, G. L., Stroud, T. K., Westhusin, M. E. and Long, C. R. (2011). Examination of DNA methyltransferase expression in cloned embryos reveals an essential role for Dnmt1 in bovine development. Molecular Reproduction and Development, 78(5), 306-317. Goll, M. G. and Bestor, T. H. (2005). Eukaryotic cytosine methyltransferases. Annual Review of Biochemistry, 74: 481-514. Guo, X., Liu, X., Xu, X., Wu, M., Zhang, X., Li, Q. et al. (2012). The expression levels of DNMT3a/3b and their relationship with meat quality in beef cattle. Molecular Biology Reports, 39(5), 5473-5479. Haig, D. (2004, January). The (dual) origin of epigenetics. In: Cold Spring Harbor Symposia on Quantitative Biology (Vol. 69, pp. 67-70). Cold Spring Harbor Laboratory Press. P. 67-70. Hayashi, K. and Yandell, D. W. (1993). How sensitive is PCR‐SSCP?. Human Mutation, 2(5): 338-346. Johanson, J. M., Berger, P. J., Tsuruta, S. and Misztal, I. (2011). A Bayesian threshold-linear model evaluation of perinatal mortality, dystocia, birth weight, and gestation length in a Holstein herd. Journal of Dairy Science, 94(1): 450-460. Kamei, Y., Suganami, T., Ehara, T., Kanai, S., Hayashi, K., Yamamoto, Y. et al. (2010). Increased expression of DNA methyltransferase 3a in obese adipose tissue: studies with transgenic mice. Obesity, 18(2), 314-321. Kulis, M. and Esteller, M. (2010). DNA methylation and cancer. Advances in genetics, 70: 27-56. Lee, J. T. (2003). Molecular links between X-inactivation and autosomal imprinting: X-inactivation as a driving force for the evolution of imprinting?. Current Biology, 13(6): R242-R254. Li, E., Beard, C. and Jaenisch, R. (1993). Role for DNA methylation in genomic imprinting. Nature, 366:362–365. Li, E., Bestor T. H. and Jaenisch R. (1992). Targeted mutation of the DNA methyltransferase gene results in embryonic lethality. Cell, 69: 915–926. Liu, X., Guo, X. Y., Xu, X. Z., Wu, M., Zhang, X., Li, Q. et al. (2012). Novel single nucleotide polymorphisms of the bovine methyltransferase 3b gene and their association with meat quality traits in beef cattle. Genetics and Molecular Research, 11: 2569-2577. Lorincz, M. C., Dickerson, D. R., Schmitt, M. and Groudine, M. (2004). Intragenic DNA methylation alters chromatin structure and elongation efficiency in mammalian cells. Nature Structural and Molecular Biology, 11(11): 1068. Lucifero, D., La Salle, S., Bourc'his, D., Martel, J., Bestor, T. H., Trasler, J. M. (2007). Coordinate regulation of DNA methyltransferase expression during oogenesis. BMC Developmental Biology, 7: 36. Meijering, A. (1984). Dystocia and stillbirth in cattle-A review of causes, relations and implications. Livestock Production Science, 11(2): 143-177. Mmereole, F. U. and Obinne, J. I. (2010). Relationship of the body weight and linear measurements of the west African Dwarf (WAD) sheep under the humid environment of Nigeria. Agricultura Tropica et Subtropica, 43(1): 64-67. Moore, L. D., Le, T. and Fan, G. (2013). DNA methylation and its basic function. Neuropsychopharmacology, 38(1): 23. Okano, M., Bell, D. W., Haber, D. A. and Li, E. (1999). DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. Cell, 99: 247-257. Olson, K. M., Cassell, B. G., McAllister, A. J. and Washburn, S. P. (2009). Dystocia, stillbirth, gestation length, and birth weight in Holstein, Jersey, and reciprocal crosses from a planned experiment. Journal of Dairy Science, 92(12): 6167-6175. Saradalekshmi, K. R., Neetha, N. V., Sathyan, S., Nair, I. V., Nair, C. M. and Banerjee, M. (2014). DNA methyl transferase (DNMT) gene polymorphisms could be a primary event in epigeneticsusceptibility to schizophrenia. PLoS ONE, 9: e98182. Seidel, C., Florean, C., Schnekenburger, M., Dicato, M. and Diederich, M. (2012). Chromatin-modifying agents in anti-cancer therapy. Biochemistry, 94: 2264-2279. Sleutels, F., Zwart, R. and Barlow, D. P. (2002). The non-coding Air RNA is required for silencing autosomal imprinted genes. Nature, 415: 6873- 810. Takahashi, K., Kobayashi, T. and Kanayama, N. (2000). p57Kip2 regulates the proper development of labyrinthine and spongiotrophoblasts. Molecular Human Reproduction, 6(11): 1019-1025. Tidball, J. G. and Spencer M. J. (2002). Expression of a calpastatin transgene slows muscle wasting and obviates changes in myosin isoform expression during murine muscle disuse. The Journal of Physiology, 545(3): 819-828. Wang, Q., Ning, X., Zhang, Q., Liu, F., Wu, H., Zhang, Y. et al. (2014). Molecular characterization of two glutathione peroxidase genes in Mytilus galloprovincialis and their transcriptional responses to sub-chronic arsenate and cadmium exposure. ISJ, 11: 149-162. Xiang, G., Zhenkun, F., Shuang, C., Jie, Z., Hua, Z., Wei, J. et al. (2010). Association of DNMT1 gene polymorphisms in exons with sporadic infiltrating ductal breast carcinoma among Chinese Han women in the Heilongjiang Province. Clinical Breast Cancer, 10: 373-377. Ye, C., Beeghly-Fadiel, A., Lu, W., Long, J., Shu, X. O., Gao, Y. T. et al. (2010). Two-stage case–control study of DNMT-1 and DNMT-3B gene variants and breast cancer risk. Breast Cancer Research and Treatment, 121: 765-769. Zhong, X., Peng, Y., Yao, C., Qing, Y., Yang, Q., Guo, X., Xie, W., Zhao, M., Cai, X., and Zhou, J. G. (2016). Association of DNA methyltransferase polymorphisms with susceptibility to primary gouty arthritis. Biomedical Reports, 5: 467-472. | ||
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