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پاسخ های فیزیولوژیکی-بیوشیمیایی بذور زوال یافته بادام زمینی به پیش‌تیمار پراکسیدهیدروژن در جریان تنش خشکی | ||
| علوم و فناوری بذر ایران | ||
| مقاله 1، دوره 9، شماره 2 - شماره پیاپی 18، شهریور 1399، صفحه 1-17 اصل مقاله (784.31 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/ijsst.2019.121357.1187 | ||
| نویسندگان | ||
| حسین رضا روحی1؛ علی سپهری* 2 | ||
| 1دانشگاه بوعلی سینا | ||
| 2گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه بوعلی سینا | ||
| چکیده | ||
| زوال بذر یکی از عوامل مهم کاهنده کیفیت فیزیولوژیکی بذر است که در شرایط نامساعد محیطی خسارت آن شدت می‌یابد. اثر پراکسید هیدروژن در بهبود وضعیت فیزیولوژیکی و فعالیت آنزیم های آنتی اکسیدان بذور زوال یافته بادام زمینی (Arachis hypogaea L.) تحت تنش خشکی طی آزمایشی به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار بررسی شد. پیش‌تیمار پراکسید هیدروژن در غلظت‌های صفر (هیدروپرایمینگ)، 50، 100 و 150 میکرو-مولار و سطوح خشکی صفر، 4/0-، 6/0- و 8/0- مگاپاسکال مورد ارزیابی قرار گرفت. نتایج نشان داد که پیش‌تیمار بذور با پراکسید هیدروژن در سطوح مختلف خشکی موجب افزایش درصد جوانه زنی، سرعت جوانه‌زنی، بنیه بذر، طول ساقه‌چه، طول ریشه‌چه، کربوهیدرات‌های محلول، پروتئین‌های محلول و فعالیت آنزیم های کاتالاز، سوپراکسید دیسموتاز و آسکوربات پراکسیداز و کاهش متوسط زمان جوانه‌زنی، هدایت الکتریکی و محتوای مالون دی‌آلدهید شد. در پتانسیل 8/0- مگاپاسکال، پیش‌تیمار بذور با غلظت‌های 50 و 100میکرومولار پراکسید هیدروژن سبب افزایش سرعت جوانه زنی، شاخص بنیه بذر، کربوهیدرات‌ها و پروتئین‌های محلول به‌ترتیب به‌میزان 89/27، 24/93، 42/71، 12/46 و 06/27، 73/76 ، 26/30 ، 04/51 درصد و همچنین افزایش فعالیت آنزیم‌های کاتالاز، سوپراکسید دیسموتاز و آسکوربات پراکسیداز به‌ترتیب به میزان 37/26، 79/07،8/39 و 43/15، 28/5 ، 51/22 درصد شد. بنابراین استفاده از 50 میکرومولار پراکسیدهیدروژن جهت کاهش اثرات منفی ناشی از زوال در تنش خشکی قابل توصیه است. | ||
| کلیدواژه‌ها | ||
| بادام زمینی؛ پراکسید هیدروژن؛ تنش خشکی؛ زوال بذر | ||
| عنوان مقاله [English] | ||
| Physiological-Biochemical Responses of Aged Groundnut Seeds to hydrogen peroxide priming during drought stress | ||
| نویسندگان [English] | ||
| Hossein Reza Rouhi1؛ Ali Sepehri2 | ||
| 1دانشکده کشاورزی، گروه زراعت و اصلاح نباتات | ||
| 2Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University | ||
| چکیده [English] | ||
| Seed deterioration is an important factor in reducing of physiological seed quality that it increased the severity of damage in adverse environmental conditions. The effects of hydrogen peroxide to improve physiological and antioxidant enzyme activities in aged groundnut seeds under drought stress were studied. A factorial experiment based on completely randomized design with three replications was done. Priming of different concentrations of hydrogen peroxide (0, 50, 100 and 150 µM) under 0, -0.4, -0.6 and -0.8MPa drought stress levels were evaluated. The results showed that seed priming with hydrogen peroxide in different drought levels increased some traits such as germination percentage, germination rate, vigour index, plumule and radicle length, soluble carbohydrates and protein contents and antioxidant enzymes activities (catalase, ascorbate peroxidase and superoxide dismutase). While it decreased mean germination time, electrical conductivity and malondialdehyde content in comparison to nonprime seeds. So seed priming with 50 and 100 µM of hydrogen peroxide in -0.8MPa level increased germination rate, vigor index , soluble carbohydrates and proteins, respectively by 27.89, 93.24, 71.42, 46.12, 27.06, 76.73, 30.26 and 51.04% compared to nonprimed seeds. Also, it increased activity of catalase, superoxide dismutase and ascorbate peroxidase, respectively by 26.37, 8.79, 39.07, 15.43, 5.28 and 21.51% compared to non-primed seeds. Therefore, the use of 50 mM hydrogen peroxide to reduce the negative effects caused by deterioration in drought stress is recommended. | ||
| کلیدواژه‌ها [English] | ||
| Groundnut, hydrogen peroxide, drought stress, seed deterioration | ||
| مراجع | ||
|
Ahmad, I., T. Khaliq, A. Ahmad, S.M.A. Basra, Z. Hasnain, and A. Ali. 2012. Effect of seed priming with ascorbic acid, salicylic acid and hydrogen peroxide on emergence, vigor and antioxidant activities of maize. Afr. J. Biotechnol. 11(5): 1127-1132. Amjad, H., A.M. Salman, I. Nayyer, A. Rubina, and F. Shafqat. 2003. Influence ofhydrogen peroxide on initial leaf and coleoptiles growth in etiolated wheat seedlings (Triticum aestivum L.). Asian J. Plant Sci. 2: 1121–1125. Andoh, H., and T. Kobata. 2002. Effect of seed hardening on the seedling emergence and alpha amylase activity in the grains of wheat and rice sown in dry soil. Japan. J. Crop Sci. 71: 220–225. Ashraf, M.A., R. Rasheed, I. Hussain, M. Iqbal, M.Z. Haider, S. Parveen, and M.A. Sajid. 2015. Hydrogen peroxide modulates antioxidant system and nutrient relation in maize (Zea mays L.) under water-deficit conditions. Arch. Agron. Soil Sci. 61(4): 507-523. Bailly, C. 2004. Active oxygen species and antioxidants in seed biology. Seed Sci. Res. 14: 93-107. Bailly, C., H. El-Maarouf-Bouteau, and F. Corbineau. 2008. From intracellular signaling networks to cell death: the dual role ofreactive oxygen species in seed physiology. C. R. Biol. 331: 806–814. Barba-Espin, G., P. Diaz-Vivancos, M.J.Clemente-Moreno, A.Albacete, L.Faize, M. F. Pérez-Alfocea, and J.A. Hernández. 2010. Interaction between hydrogen peroxide and plant hormones during germination and the early growth of pea seedlings. Plant Cell Environ. 33(6): 981-94. Barba-Espin, G., P. Diaz-Vivancos, D. Job, Job, and J.A. Hernández. 2011. Understanding the role of H2O2 during pea seed germination: a combined proteomic and hormone profiling approach. Plant Cell Environ. 34: 1907–1919. Barba-Espin, G., J.A. Hernández, and P. Diaz-Vivancos. 2012. Role of H2O2 in pea seed germination. Plant Signal Behav.7(2): 193–195. Bradford, M.M. 1976. A dye binding assay for protein. Analyt Biochem.72: 248-254. Cakmak, I., and W. Horst. 1991. Effect of aluminium on lipid peroxidation, superoxide dismutase, catalase and peroxidase activities in root tip of soybean (Glycine max). Plant Physiol. 83: 463–468. Cavalcanti, F.R., J.T.A. Oliveira, A.S. Martins-Miranda, R.A. Viégas, and J.A.G. Silveira. 2004. Superoxide dismutase, catalase and peroxidase activities do not confer protection against oxidative damage in salt-stressed cowpeas leaves. New Phytol. 163: 563–571. Delouche, J.C., and C.C. Baskin. 1973. Accelerated ageing technique for predicting relative storability of seed lots. Seed Sci. Technol. 1: 427-452. Fu, Y.B., Z. Ahmed, and A. Diederichsen. 2015. Towards a better monitoring of seed ageing under ex situ seed conservation. Conserv. Physiol. 3: 1-16. Delouche, J.C., and C.C. Baskin. 1973. Accelerated ageing technique for predicting relative storability of seed lots. Seed Sci. Technol. 1: 427-452. Ellis, R.A., and E.H. Roberts. 1981. The quantification of ageing and survival in orthodox seeds. Seed Sci. Technol. 9: 373–409. Giannopolitis, C., and S. Ries. 1977. Superoxid desmutase. I: Occurence in higher plant, Plant Physiol. 59: 309–314. Hampton, J.G., and D.M. TeKrony. 1995. Handbook of Vigour Test Methods. The international Seed Testing Association, Zurikh. Hancock, J., R. Desikan, J. Harrison, J. Bright, R. Hooley, and S. Neill. 2006. Doing the unexpected: proteins involved in hydrogenperoxideperception. J. Exp.Bot. 57: 1711–1718. He, L., Z. Gao, and Li, R. 2009. Pretreatment of seed with H2O2 enhances drought tolerance of wheat (Triticum aestivum L.) seedlings. African Journal of Biotechnology, 8 (22), 6151-6157. Hou, L., W. Liu, Z. Li, C. Huang, X.L. Fang, Q. Wang, and X. Liu. 2014. Identification and Expression Analysis of Genes Responsive to Drought Stress in Peanut. Russ. J. Plant Physiol. 61(6): 842–852. Irigoyen, J.J., D.W. Emerich, and M. Sanchez-Diaz. 1992. Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiol. Plant. 84: 55-60. ISTA. 2007. International Rules for Seed Testing. Seed Sci. Technol. 13: 299–520. Jisha, K.C., K. Vijayakumari, and J.T. Puthur. 2013. Seed priming for abiotic stress tolerance: an overview. Acta Physiol. Plant. 35: 1381–1396. Job, C., L. Rajjou, Y. Lovigny, M. Belghazi, and D. Job. 2005. Patternsof protein oxidation in Arabidopsis seeds and during germina-tion. Plant Physiol. 138: 790–802. Jyoti, and C.P. Malik. 2013. Seed deterioration: a review. Int. J. Life Sci. Biotech. Pharma Res. 2(3): 374–385. Kafi, M., A. Nezami, H. Hoseyni, and A. Masoomi. 2009. Physiological effects of drought stress by polyethylene glycol on germination of lentil (Lens culinaris Medik L.) genotypes. J. Iranian Field Crop Res. 1(3), 69-79 (In Farsi with English abstract). Kibinza, S., J. Bazin, C. Bailly, J.M. Farrant, F. Corbineau, and H. El-Marrouf Bouteau. 2011. Catalase is a key enzyme in seed recovery from ageing during priming. Plant Sci. 181: 309-315. Lu, S., W. Su, H. Li, and Z. Guo. 2009. Abscisic acid improves drought tolerance of triploid bermudagrass and involves H2O2 and NO-induced antioxidant enzyme activities. Plant Physiol. Biochem. 7: 132–138. Manish, K., S. Geetika, B. Renu, K. Sandeep, and J. Gaganodeep. 2010. Effect of exogenous H2O2 on antioxidant enzymes of Brassica juncea L.seedlings in relation to 24-epibrassinolide under chilling stress. Indian J. Biochem. Biophys. 47(6): 378–382. Michel, B.E., and M.R. Kaufmann. 1973. The osmotic potential of polyethylene glycol 6000. Plant Physiol. 51: 914-916. Moller, I.M., and L.J. Sweetlove. 2010. ROS signalling–specificity is required. Trends Plant Sci. 15(7): 370-374. Müller, K., A. Linkies, R.A.M. Vreeburg, and S.C. Fry. 2009. Invivo cell wall loosening by hydroxyl radicals during cross seedgermination and elongation growth. Plant Physiol. 150: 1855–1865. Nakano, Y., and K. Asada. 1981. Hydrogen peroxide scavenged by ascrobate-specific peroxidase in spinach chloroplast. Plant Cell Physiol. 22: 867–880. Nautiyal, P.C. 2009. Seed and seedling vigour traits in groundnut (Arachis hypogaea L.). Seed Sci. Technol. 37: 721-735. Qiao, W., C. Li, and L.M. Fan, 2014.Cross-talk between nitric oxide and hydrogen peroxide in plant responses to abiotic stresses. Environ. Exper. Bot. 100: 84-93. Rehman, H., H. Iqbal, S.M.A. Basra, I. Afzal, M. Farooq, A. Wakeel, and W. Ning. 2015. Seed priming improves early seedling vigor, growth and productivity of spring maize. J. Integ. Agric. 14(9): 1745–1754. Santhy, V., M. Meshram, R. Walkde, and P.R. Vijaya Kumari. 2014. Hydrogen peroxide pre-treatment for seed enhancement in Cotton (Gossypium Hirsutum L.). African J. Agric. Res. 9(25): 1982-1989. Sepehri, A., and H.R. Rouhi. 2016. Enhancement of seed vigor performance in aged groundnut (Arachis hypogaea L.) seeds by sodium nitroprusside under drought stress. Philipp. Agric. Scientist. 99(4): 339-347. Sharma, P., R.S. Dubey, and M. Pessarakli. 2012. Reactive oxygen species, oxidative damage and antioxidative defense mechanisms in plant under stressful conditions. Journal of Bot. 1-26. Tabatabaei, S.A. 2013. The Effect of priming on germination and enzyme activity of sesame (Sesamum indicum L.) seeds after accelerated aging. J. St. Physiol. Biochem. 9 (4): 132-138. Taiz, L., and Zeiger, E. 2002. Plant Physiology, 3 edn. Sinauer Associates, Inc. Publishers, Sunderland, Massachusetts. Takabe, T., A. Uchida, and T. Takabe. 2004. Effects of hydrogen peroxide on germination of Kentucky bluegrass. In Plant biology symposium, Lake Buena Vista, FL, USA (pp. 24-28). Tale Ahmad, S., and Haddad, R. 2011. Study of Silicon Effects on Antioxidant Enzyme Activities and Osmotic Adjustment of Wheat under Drought Stress. Czech J. Genet. Plant Breed. 47 (1): 17–27. Tanou, G., C. Job, L. Rajjou, E. Arc, M. Belghazi, G. Diamantidis, A. Molassiotis, and D. Job, 2009. Proteomics reveals the overlappingroles ofhydrogen peroxide and nitric oxide in the acclimationof citrus plants to salinity. The Plant J. 60: 795–804. Varier, A., A.K., Vari, and M. Dadlani. 2010. The subcellular basis of seed priming. Current Sci. 99(4): 450-456. Xia, F., X.Wang, M. Li, and P. Mao. 2015. Mitochondrial structural and antioxidant system responses to aging in oat (Avena sativa L.) seeds with different moisture contents. Plant Physiol. Biochem. 94: 122-129. Yadav, P.V., M. Kumari, and Z. Ahmed. 2011. Seed priming mediated germination improvement and tolerance to subsequent exposure to cold and salt stress in capsicum. Res. J.Seed Sci. 4: 125–136. Zhang, M., Z. Wang, L. Yuan, C. Yin, J. Cheng, L. Wang, J. Huang, and H. Zhang. 2014. Osmopriming improves tomato seed vigor under aging and salinity stress. Afric. J. Biotech. 11(23): 6305-6311. | ||
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