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پاسخ های فیزیولوژیکی-بیوشیمیایی بذور زوال یافته بادام زمینی به پیش‌تیمار پراکسیدهیدروژن در جریان تنش خشکی | ||
| علوم و فناوری بذر ایران | ||
| مقاله 1، دوره 9، شماره 2 - شماره پیاپی 18، شهریور 1399، صفحه 1-17 اصل مقاله (784.31 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22034/ijsst.2019.121357.1187 | ||
| نویسندگان | ||
| حسین رضا روحی1؛ علی سپهری* 2 | ||
| 1دانشگاه بوعلی سینا | ||
| 2گروه زراعت و اصلاح نباتات، دانشکده کشاورزی، دانشگاه بوعلی سینا | ||
| چکیده | ||
| زوال بذر یکی از عوامل مهم کاهنده کیفیت فیزیولوژیکی بذر است که در شرایط نامساعد محیطی خسارت آن شدت می‌یابد. اثر پراکسید هیدروژن در بهبود وضعیت فیزیولوژیکی و فعالیت آنزیم های آنتی اکسیدان بذور زوال یافته بادام زمینی (Arachis hypogaea L.) تحت تنش خشکی طی آزمایشی به‌صورت فاکتوریل در قالب طرح کاملاً تصادفی با سه تکرار بررسی شد. پیش‌تیمار پراکسید هیدروژن در غلظت‌های صفر (هیدروپرایمینگ)، 50، 100 و 150 میکرو-مولار و سطوح خشکی صفر، 4/0-، 6/0- و 8/0- مگاپاسکال مورد ارزیابی قرار گرفت. نتایج نشان داد که پیش‌تیمار بذور با پراکسید هیدروژن در سطوح مختلف خشکی موجب افزایش درصد جوانه زنی، سرعت جوانه‌زنی، بنیه بذر، طول ساقه‌چه، طول ریشه‌چه، کربوهیدرات‌های محلول، پروتئین‌های محلول و فعالیت آنزیم های کاتالاز، سوپراکسید دیسموتاز و آسکوربات پراکسیداز و کاهش متوسط زمان جوانه‌زنی، هدایت الکتریکی و محتوای مالون دی‌آلدهید شد. در پتانسیل 8/0- مگاپاسکال، پیش‌تیمار بذور با غلظت‌های 50 و 100میکرومولار پراکسید هیدروژن سبب افزایش سرعت جوانه زنی، شاخص بنیه بذر، کربوهیدرات‌ها و پروتئین‌های محلول به‌ترتیب به‌میزان 89/27، 24/93، 42/71، 12/46 و 06/27، 73/76 ، 26/30 ، 04/51 درصد و همچنین افزایش فعالیت آنزیم‌های کاتالاز، سوپراکسید دیسموتاز و آسکوربات پراکسیداز به‌ترتیب به میزان 37/26، 79/07،8/39 و 43/15، 28/5 ، 51/22 درصد شد. بنابراین استفاده از 50 میکرومولار پراکسیدهیدروژن جهت کاهش اثرات منفی ناشی از زوال در تنش خشکی قابل توصیه است. | ||
| کلیدواژه‌ها | ||
| بادام زمینی؛ پراکسید هیدروژن؛ تنش خشکی؛ زوال بذر | ||
| عنوان مقاله [English] | ||
| Physiological-Biochemical Responses of Aged Groundnut Seeds to hydrogen peroxide priming during drought stress | ||
| نویسندگان [English] | ||
| Hossein Reza Rouhi1؛ Ali Sepehri2 | ||
| 1دانشکده کشاورزی، گروه زراعت و اصلاح نباتات | ||
| 2Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University | ||
| چکیده [English] | ||
| Seed deterioration is an important factor in reducing of physiological seed quality that it increased the severity of damage in adverse environmental conditions. The effects of hydrogen peroxide to improve physiological and antioxidant enzyme activities in aged groundnut seeds under drought stress were studied. A factorial experiment based on completely randomized design with three replications was done. Priming of different concentrations of hydrogen peroxide (0, 50, 100 and 150 µM) under 0, -0.4, -0.6 and -0.8MPa drought stress levels were evaluated. The results showed that seed priming with hydrogen peroxide in different drought levels increased some traits such as germination percentage, germination rate, vigour index, plumule and radicle length, soluble carbohydrates and protein contents and antioxidant enzymes activities (catalase, ascorbate peroxidase and superoxide dismutase). While it decreased mean germination time, electrical conductivity and malondialdehyde content in comparison to nonprime seeds. So seed priming with 50 and 100 µM of hydrogen peroxide in -0.8MPa level increased germination rate, vigor index , soluble carbohydrates and proteins, respectively by 27.89, 93.24, 71.42, 46.12, 27.06, 76.73, 30.26 and 51.04% compared to nonprimed seeds. Also, it increased activity of catalase, superoxide dismutase and ascorbate peroxidase, respectively by 26.37, 8.79, 39.07, 15.43, 5.28 and 21.51% compared to non-primed seeds. Therefore, the use of 50 mM hydrogen peroxide to reduce the negative effects caused by deterioration in drought stress is recommended. | ||
| کلیدواژه‌ها [English] | ||
| Groundnut, hydrogen peroxide, drought stress, seed deterioration | ||
| مراجع | ||
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