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Biological investigation of Lilium ledebourii in Iran | ||
| Rostaniha | ||
| دوره 21، شماره 2 - شماره پیاپی 60، اسفند 2020، صفحه 141-153 اصل مقاله (559.7 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22092/botany.2020.341949.1190 | ||
| نویسندگان | ||
| Salman Maleki Choreh1؛ Fatemeh Zarinkamar* 2؛ Mehrdad Nazari3 | ||
| 1MSc Student of Cellular and Developmental Biology, Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran | ||
| 2Associate Prof., Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran | ||
| 3PhD in Plant Physiology, Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran | ||
| چکیده | ||
| Lilium ledebourii is a rare Asian species and is an endemic plant of Iran which grows in Gilan province in Damash highlands. In this study, the structural features of flower including the study of sections such as carpel, stamen, and tepals were performed. This plant had a full flower that consisted of six tepals (three petals and three petal-like sepals), six free stamens and a 3-lobed gynoecium. In the cross-section of tepals, the stomata as well as cells containing anthocyanins were observed. In stamens, anther (epidermis, endothecium, middle layers and an innermost tapetum), and filament were observed. In the gynoecium, ovary (ovary wall, locule, funiculus, anatropous ovule, different arrangements of vascular bundles, transmitting tissue and stomata), style (stylar canal and transmitting tissue), and dry stigma with elongated papillae tissue were observed. Besides, the presence of alkaloids in the bulb was tested using Wagner and Dragendorff reagents and the total alkaloid content in three types of methanol, ethanol, and butanol extraction was investigated. The ethanol extract had a significant difference with other extractions. The methanol extract was chosen for further investigation by gas chromatography-mass spectrometry (GC-MS) and two compounds of Pterin-6-carboxylic acid, and Pyrrolizin-1,7-dione-6-carboxylic acid, methyl (ester) were detected. | ||
| کلیدواژه‌ها | ||
| Alkaloid؛ anatropous ovule؛ Liliaceae؛ papillae؛ petal-like sepal؛ tepal | ||
| عنوان مقاله [English] | ||
| بررسی زیست‌شناسی سوسن چلچراغ در ایران | ||
| نویسندگان [English] | ||
| سلمان ملکی چره1؛ فاطمه زرین کمر2؛ مهرداد نظری3 | ||
| 1دانشجوی کارشناسی ارشد زیست‌شناسی سلولی و تکوینی، گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران | ||
| 2دانشیار گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران | ||
| 3دکترای فیزیولوژی گیاهی، گروه علوم گیاهی، دانشکده علوم زیستی، دانشگاه تربیت مدرس، تهران، ایران | ||
| چکیده [English] | ||
| سوسن چلچراغ (Lilium ledebourii) یک گونه نادر آسیایی از تیره سوسنیان است که بومی ایران بوده و در استان گیلان، در ارتفاعات منطقه داماش رشد می‌کند. در این مطالعه، ویژگی‌های ساختاری گل مشتمل بر مطالعه بخش‌هایی از قبیل برچه، پرچم و گلپارها انجام شد. این گیاه دارای یک گل کامل متشکل از شش گلپار (سه گلبرگ و سه کاسبرگ گلبرگ‌آسا)، شش پرچم آزاد و تخمدان سه برچه‌ای است. در سطح مقطع گلپارها، روزنه‌ها و همچنین سلول‌های حاوی آنتوسیانین‌ها مشاهده شدند. در پرچم‌ها، بساک (اپیدرم، لایه مکانیکی لایه‌های میانی و سلول‌های لایه پرستار میانی و میله مشاهده شد. در مادگی، تخمدان (دیواره، حفره، بند تخمک، تخمک آناتروپ، گروه‌های مختلف دسته‌های آوندی، بافت انتقال و روزنه‌ها)، خامه (کانال خامه و بافت انتقال دهنده) و کلاله خشک با بافت پاپیلای کشیده مشاهده شد. علاوه بر این، حضور آلکالوییدها در پیاز گل با استفاده از معرف‌های واگنر و دراژندورف مورد آزمایش قرار گرفت و محتوای کل آلکالوییدها در سه نوع عصاره متانولی، اتانولی و بوتانولی بررسی شد. عصاره اتانولی با سایر عصاره‌ها تفاوت معنی‌داری داشت. عصاره متانولی برای بررسی بیشتر با استفاده از کروماتوگرافی گازی-طیف‌سنجی جرمی (GC-MS) انتخاب شد و دو ترکیب Pterin-6-carboxylic acid و Pyrrolizin-1,7-dione-6-carboxylic acid, methyl (ester) شناسایی گردیدند. | ||
| کلیدواژه‌ها [English] | ||
| آلکالویید, تخمک آناتروپ, پاپیله, سوسنیان, کاسبرگ شبیه گلبرگ, گلپار | ||
| مراجع | ||
|
Altameme, H.J. 2017. A Chemical composition of Halophyte plant Frankenia pulverulenta L. (Frankeniaceae) in Iraq depending on GC-MS and FT-IR techniques. Journal of Chemical and Pharmaceutical Sciences 10: 26–33. Azadi, P. & Khosh-Khui, M. 2007. Micropropagation of Lilium ledebourii (Baker) Boiss as affected by plant growth regulator, sucrose concentration, harvesting season and cold treatments. Electronic Journal of Biotechnology 10: 582–591. Buckingham, J., Baggaley, K.H., Roberts, A.D. & Szabo, L.F. 2010. Dictionary of Alkaloids with CD-ROM. CRC Press, London, 605 pp. Chebouat, E., Dadamoussa, B., Kabouche, A., Allaoui, M., Gouamid, M. & Gherraf, N. 2013. Gas chromatography-mass spectrometry (GC-MS) analysis of the crude alkaloid extract of Ziziphus mauritiana Lam., grown in Algerian. Journal of Medicinal Plants Research 7: 1511–1514. Devi, N.I., Kumar, S.N. & Rajaram, C. 2016. Evaluation of hepatoprotective activity of Lilium candidum L. in experimental animal models. World Journal of Pharmaceutical Research 5: 725–749. Dhyani, A., Bahuguna, Y.M., Semwal, D.P., Nautiyal, B.P. & Nautiyal, M.C. 2009. Anatomical features of Lilium polyphyllum D. Don ex Royle (Liliaceae). Journal of American Science 5: 85–90. Erdemoglu, N., Ozkan, S., Duran, A. & Tosun, F. 2009. GC-MS analysis and antimicrobial activity of alkaloid extract from Genista vuralii. Pharmaceutical Biology 47: 81–85. Farsam, H., Amanlou, M., Amin, G., Nezamivand-Chegini, G., Salehi-Surmaghi, M.H. & Shafiee, A. 2003. Anatomical and phytochemical study of Lilium ledebourii (Baker) Boiss., a rare endemic species in Iran. DARU Journal of Pharmaceutical Sciences 11: 164–170. Fazel, S., Hamidreza, M., Rouhollah, G. & Verdian-rizi, M. 2010. Spectrophotometric determination of total alkaloids in some Iranian medicinal plants. The Society for Advancement of Horticulture 12: 69–70. Fileto-Pérez, H.A., Rutiaga-Quiñones, O.M., Sytsma, M.D., Lorne, I.M., Luo, W., Pankow, J.F. & Rutiaga-Quiñones, J.G. 2015. GC/MS analysis of some extractives from Eichhornia crassipes. BioResources 10: 7353–7360. Firdouse, S. & Alam, P. 2011. Phytochemical investigation of extract of Amorphophallus campanulatus tubers. International Journal of Phytomedicine 3: 32–35. Glimn-Lacy, J. & Kaufman, P.B. 2006. Botany illustrated: introduction to plants, major groups, flowering plant families. Springer, New York, US. 128 pp. Güven, S., Okur, S., Demırel, M., Coskuncelebi, K., Makbul, S. & Beyazoğlu, O. 2014. Pollen morphology and anatomical features of Lilium (Liliaceae) taxa from Turkey. Biologia 69: 1122–1133. Hayashi, K. & Kawano, S. 2000. Molecular systematics of Lilium and allied genera (Liliaceae): phylogenetic relationships among Lilium and related genera based on the rbcL and matK gene sequence data. Plant Species Biology 15: 73–93. Hussein, H.M., Hameed, I.H. & Ubaid, J.M. 2016. Analysis of the secondary metabolite products of Ammi majus and evaluation anti-insect activity. International Journal of Pharmacognosy and Phytochemical Research 8: 1192–1189. Kaviani, B., Dehkaei, M.P., Darabi, A., Rafizadeh, A. & Rahmati, B. 2008. The anatomical properties of endemic Lilium ledebourii (Baker) Bioss. (Liliaceae) species. International Journal of Botany 4: 62–66. Kosenko, V. 1999. Contributions to the pollen morphology and taxonomy of the Liliaceae. Grana 38: 20–30. Kruse, L.H., Stegemann, T., Sievert, C. & Ober, D. 2017. Identification of a second site of pyrrolizidine alkaloid biosynthesis in comfrey to boost plant defense in floral stage. Plant Physiology 174: 47–55. McLean, R.S. & Ireland, E.J. 1940. Rapid staining methods in plant histology. Journal of the American Pharmaceutical Association 29: 318–321. Meng, Y., Wang, S., Cai, R., Jiang, B. & Zhao, W. 2015. Discrimination and content analysis of Fritillaria using near infrared spectroscopy. Journal of Analytical Methods in Chemistry 1–9. doi:10.1155/2015/752162. Meshram, A., Kumar, A. & Srivastava, N. 2015. Gas chromatography-mass spectrometry (GC-MS) analysis of alkaloids isolated from Epipremnum aureum (Linden and Andre) bunting. International Journal of Pharmaceutical Sciences and Research 6: 337–342. Mimaki, Y. & Sashida, Y. 1990. Sterodial Saponins and Alkaloids from the Bulbs of Lilium brownii var. colchesteri. Chemical and Pharmaceutical Bulletin 38: 3055–3059. Nishikawa, T., Okazaki, K., Uchino, T., Arakawa, K. & Nagamine, T. 1999. A molecular phylogeny of Lilium in the internal transcribed spacer region of nuclear ribosomal DNA. Journal of Molecular Evolution 49: 238–249. Ozen, F., Temeltas, H. & Aksoy, O. 2012. The antomy and morphology of the medicinal plant, Lilium candidum L. (Liliaceae), distributed in Marmara region of Turky. Pakistan Journal of Botany 44: 1185–1192. Roy, S.D., Dash, S., Dasgupta, R.K. & Chakraborty, J. 2019. Modulatory potential on immune system of a new alkaloid isolated from Coccinea cordifolia root. International Journal of Biological Macromolecules 121: 643–649. Rudall, P.J. 2007. Anatomy of flowering plants: an introduction to structure and development. Cambridge University Press, pp. 75–93. Shiboob, M.H. 2018. Antioxidant activity of phenolic and alkaloid fractions accumulated in Artemisia judaica and Artemisia herba alba. Journal of Natural Remedies 17: 154–164. Simpson, M.G. 2010. Plant systematics. 2nd edn. London: Elsevier-Academic Press. 180 pp. Singh, S. & Walles, B. 1992. The ovarian transmitting tissue in Lilium regale. International Journal of Plant Sciences 153: 205–211. Singh, V. 2014. Flora of Madhya Pradesh (Western part). Scientific Publishers, Jodhpur. 449 pp. Tabasum, S., Khare, S. & Jain, K. 2016. Spectrophotometric quantification of total phenolic, flavonoid and alkaloid contents of Abrus precatorius L. seeds. Asian Journal of Pharmaceutical and Clinical Research 9: 371–374. Vadi, R., Manisha, V. & Swati, K. 2017. Hansraj (Adiantum capillus veneris Linn.): A systematic review on its ethnobotany, phytochemical and pharmacological profile. International Journal of Ayurveda and Pharma Research 5: 5–21. Xing, M., Du, Y., Wang, X., Niu, L. & Chen, X. 2010. A simplified paraffin embedding method for small botanical samples. Biotechnic & Histochemistry 85: 241–246. Yamagishi, M. & Akagi, K. 2013. Morphology and heredity of tepal spots in Asiatic and Oriental hybrid lilies (Lilium spp.). Euphytica 194: 325–334. Zishan, A., Anwar, S. & Shiwali, S. 2017. Evaluation of in vitro Antioxidant Activity, HPLC and GC-MS Analysis Along With Chemoprofiling of Decalepis arayalpathra: A Critically Endangered Plant of Western Ghats, India. Rendiconti Lincei 28: 711–720. | ||
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