| تعداد نشریات | 282 |
| تعداد نشریات سازمان | 80 |
| تعداد شمارهها | 3,196 |
| تعداد مقالات | 35,423 |
| تعداد مشاهده مقاله | 52,252,800 |
| تعداد دریافت فایل اصل مقاله | 91,783,236 |
Variation of Main Alkaloids and Fatty Acids among Five Natural Populations of Peganum harmala L. from Iran | ||
| Journal of Medicinal plants and By-products | ||
| مقاله 5، دوره 10، شماره 1، تیر 2021، صفحه 45-52 اصل مقاله (644.94 K) | ||
| نوع مقاله: Research Paper | ||
| شناسه دیجیتال (DOI): 10.22092/jmpb.2020.128202.1151 | ||
| نویسندگان | ||
| Sepideh Niazi؛ Sattar Tahmasebi Enferadi* ؛ Hadi Ghaderitabar | ||
| Department of Plant Molecular Biotechnology, Institute of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran | ||
| چکیده | ||
| Alkaloids and fatty acids extracted from plant species are considered as popular ingredients in pharmaceutical products. The medicinal herb Peganum harmala L., native toarid and semi-arid rangeland, is known as an excellent source of β-carboline alkaloids and some important essential fatty acids. In order to extend the knowledge of phytochemical biodiversity of P. harmala, main alkaloids and fatty acids contents of the populations collected from five different parts of Iran were investigated. Harmine and harmaline, that are the main β-carboline alkaloids of P. harmala seed extract, were identified by FTIR and quantified by HPLC. Significant quantitative differences (p ≤ 0.01) were observed in harmaline (295-354.16 mg/g dry extract) and harmine (257.91-304.66 mg/g dry extract) contents among studied populations. Furthermore, seed oil was extracted and analysis of fatty acids was performed quantitatively using GC method. The result showed that the average oil content of P. harmala was 15.82±1.06%, with no significant difference (p ≤ 0.05) among the samples. The most abundant fatty acid in all analyzed samples was linoleic acid (C18:2) (54.33-60.53% of the total fatty acids). Statistical analysis revealed significant differences (p ≤ 0.05) among the populations for linoleic acid (C18:2) (58.21±2.70%) and palmitic acid (C16:0) (9.99±1.19%) contents. Additionally, all the samples were rich in unsaturated fatty acids and β-carbolines, harmaline and harmine. These data indicate that the amounts of major alkaloids and fatty acids vary considerably among the P. harmala’s natural populations, which could be potential sources of these important phytochemicals for pharmaceutical and industrial purposes. | ||
| کلیدواژهها | ||
| Peganum harmala L؛ Fatty acids؛ β-carboline alkaloids؛ Harmine؛ Harmaline | ||
| مراجع | ||
|
1. Ehsanpour A, Saadat E. Plant regeneration from hypocotyl culture of Peganum harmala. Pakistan J of Botany. 2002;34:253-6.
2. Goel N, Singh N, Saini R. Efficient in vitro multiplication of Syrian Rue (Peganum harmala L.) using 6-benzylaminopurine pre-conditioned seedling explants. Nat Sci. 2009;7:129-34.
3. Yousefi R, Ghaffarifar F, Asl AD. The effect of Alkanna tincturia and Peganum harmala extracts on Leishmania major (MRHO/IR/75/ER) in vitro. Iranian J of Parasitology. 2009:40-7.
4. Mahmoudian M, Jalipour H, Salehian Dardashti P. Toxicity of Peganum harmala - review and a case report. Iranian J of Pharmacology and Therapeutics. 2002;1:1-0.
5. Chatterjee A, Ganguly M. Alkaloidal constituents of Peganum harmala and synthesis of the minor alkaloid deoxyvascinone. Phytochemistry. 1968;7:307-11.
6. Sharaf M, El-Ansari MA, Matlin SA, Saleh NA. Four flavonoid glycosides from Peganum harmala. Phytochemistry. 1997;44:533-6.
7. Movafeghi A, Abedini M, Fathiazad F, Aliasgharpour M, Omidi Y. Floral nectar composition of Peganum harmala L. Natural Product Res. 2009;23:301-8.
8. Moussa TA, Almaghrabi OA. Fatty acid constituents of Peganum harmala plant using Gas Chromatography–Mass Spectroscopy. Saudi J Biological Sci. 2016;23:397-403.
9. Wagner H, Bladt S. Plant drug analysis: a thin layer chromatography atlas, Springer Sci and Business Media, 1996.
10. Cheng J, Mitchelson KR. Improved separation of six harmane alkaloids by high-performance capillary electrophoresis. J Chromatography A. 1997;761:297-305.
11. Kartal M, Altun M, Kurucu S. HPLC method for the analysis of harmol, harmalol, harmine and harmaline in the seeds of Peganum harmala L. J Pharmaceutical Biomed Analysis. 2003;31:263-9.
12. Hashimoto Y, Kawanishi K, Moriyasu M. Forensic Chemistry of Alkaloids by Chromatographic Analysis. Alkaloids: Chem Pharmaco Elsevier, 1988, pp. 1-77.
13. Picada JN, da Silva KV, Erdtmann B, Henriques AT, Henriques JA. Genotoxic effects of structurally related β-carboline alkaloids. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis. 1997;379:135-49.
14. Takahashi M, Nagashima M, Suzuki J, Seto T, Yasuda I, Yoshida T. Creation and application of psychoactive designer drugs data library using liquid chromatography with photodiode array spectrophotometry detector and gas chromatography–mass spectrometry. Talanta. 2009;77:1245-72.
15. Lamchouri F, Settaf A, Cherrah Y, Zemzami M, Lyoussi B, Zaid A, et al. Antitumour principles from Peganum harmala seeds. Therapie. 1999;54:753-8.
16. Lamchouri F, Settaf A, Cherrah Y, El MH, Tligui N, Lyoussi B, et al., editors. Experimental toxicity of Peganum harmala seeds. Annales Pharmaceu Françaises. 2002.
17. Di Giorgio C, Delmas F, Ollivier E, Elias R, Balansard G, Timon-David P. In vitro activity of the β-carboline alkaloids harmane, harmine, and harmaline toward parasites of the species Leishmania infantum. Experimental Parasitology. 2004;106:67-74.
18. Arshad N, Zitterl‐Eglseer K, Hasnain S, Hess M. Effect of Peganum harmala or its β-carboline alkaloids on certain antibiotic resistant strains of bacteria and protozoa from poultry. Phytotherapy Research: An International J Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. 2008;22:1533-8.
19. Hamden K, Carreau S, Ayadi F, Masmoudi H, Abdelfattah E. Inhibitory effect of estrogens, phytoestrogens, and caloric restriction on oxidative stress and hepato-toxicity in aged rats. Biomedical and Environmental Sci. 2009;22:381-7.
20. Herraiz T, González D, Ancín-Azpilicueta C, Arán V, Guillén H. β-Carboline alkaloids in Peganum harmala and inhibition of human monoamine oxidase (MAO). Food and Chemical Toxicology. 2010;48:839-45.
21. Mayad E, Hassani L, Ferji Z, Chebli B, El-Hadek M. Quantitative and qualitative analysis by HPLC of major Peganum harmala alkaloids at different stages of development. J Natural Sci Res. 2013;3:104-11.
22. Mabberley DJ. The plant-book: a portable dictionary of the vascular plants, Cambridge university press. 1997.
23. Apostolico I, Aliberti L, Caputo L, De Feo V, Fratianni F, Nazzaro F, et al. Chemical composition, antibacterial and phytotoxic activities of Peganum harmala seed essential oils from five different localities in Northern Africa. Mol. 2016;21:1235.
24. Valares Masa C, Alías Gallego J, Chaves Lobon N, Sosa Diaz T. Intra-population variation of secondary metabolites in Cistus ladanifer L. Mol. 2016;21:945.
25. Seyed Hassan Tehrani S, Hashemi Sheikh Shabani S, Tahmasebi Enferadi S, Rabiei Z. Growth inhibitory impact of Peganum harmala L. on two breast cancer cell lines. Iranian J Biotech. 2014;12:8-14.
26. Parvini F, Zeinanloo AA, Ebrahimie E, Tahmasebi‐Enferadi S, Hosseini Mazinani M. Differential expression of fatty acid desaturases in Mari and Shengeh olive cultivars during fruit development and ripening. European J Lipid Sci Technol. 2015;117:523-31.
27. Silverstein RM, Bassler GC. Spectrometric identification of organic compounds. J Chem Education. 1962;39:546.
28. Sobhani AM, Ebrahimi S-A, Mahmoudian M. An in vitro evaluation of human DNA topoisomerase I inhibition by Peganum harmala L. seeds extract and its beta-carboline alkaloids. J Pharm Pharm Sci. 2002;5:19-23.
29. Wang X, Geng Y, Wang D, Shi X, Liu J. Separation and purification of harmine and harmaline from Peganum harmala using pH‐zone‐refining counter‐current chromatography. J Sep Sci. 2008;31:3543-7.
30. Pavarini DP, Pavarini SP, Niehues M, Lopes NP. Exogenous influences on plant secondary metabolite levels. Animal Feed Sci Technol. 2012;176:5-16.
31. Mathur M, Sundaramoorthy S. Mineral Composition in Corchorus depressus at Heterogeneous Environmental Conditions and Their Relationships with Bottom‐Up, Top‐Down, and Plant Metabolite Factors. Communications in Soil Science and Plant Analysis. 2009;40:2028-43.
32. Shukla S, Yadav HK, Rastogi A, Mishra BK, Singh SP. Alkaloid diversity in relation to breeding for specific alkaloids in opium poppy (Papaver somniferum L.). Czech J Gen Plant Breed. 2010;46:164-9.
33. Mathur M, Sundaramoorthy S. Ethnopharmacological studies of Tribulus terrestris (Linn). in relation to its aphrodisiac properties. African J Traditional, Complementary Alternative Med. 2013;10:83-94.
34. COŞGE B, GÜRBÜZ B, KIRALAN M. Oil Content and Fatty Acid Composition of Some Safflower (Carthamus tinctorius L.) Varieties Sown in Spring and Winter. Int J Natural Engin Sci. 2007;1.
35. Nehdi IA, Sbihi HM, Tan CP, Al-Resayes SI. Seed oil from Harmal (Rhazya stricta Decne) grown in Riyadh (Saudi Arabia): a potential source of δ-tocopherol. J Saudi Chem Soci. 2016;20:107-13.
36. Esmaeili A, Shaykhmoradi F, Naseri R. Comparison of oil content and fatty acid composition of native olive genotypes in different region of Liam, Iran. Int J Agric Crop Sci. 2012;4:434-8.
37. Vosoughkia M, Hossainchi Ghareaghag L, Ghavami M, Gharachorloo M, Delkhosh B. Evaluation of oil content and fatty acid composition in seeds of different genotypes of safflower. International J Agric Sci Res. 2012;2:59-66.
38. Turan S, Topcu A, Karabulut I, Vural H, Hayaloglu AA. Fatty acid, triacylglycerol, phytosterol, and tocopherol variations in kernel oil of Malatya apricots from Turkey. J Agric food Chem. 2007;55:10787-94.
39. Green A. Genetic control of polyunsaturated fatty acid biosynthesis in flax (Linum usitatissimum) seed oil. Theoretical App Gen. 1986;72:654-61. | ||
|
آمار تعداد مشاهده مقاله: 1,117 تعداد دریافت فایل اصل مقاله: 1,133 |
||