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A Simple, Quick, Efficient and Cost-effective Method for Isolating Genomic DNA from Medicinal Plant Lemon Balm | ||
| Journal of Medicinal plants and By-products | ||
| دوره 9، شماره 1، تیر 2020، صفحه 71-78 اصل مقاله (440.56 K) | ||
| نوع مقاله: Research Article | ||
| شناسه دیجیتال (DOI): 10.22092/jmpb.2020.122077 | ||
| نویسندگان | ||
| Sara Ghaffarian1، 2؛ Seyed Abolghasem Mohammadi* 3، 4 | ||
| 1Departement of Biology, Faculty of Natural Science, Azarbaijan Shahid Madani University, Tabriz, Iran | ||
| 2Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran. | ||
| 3Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Tabriz, Tabriz, Iran | ||
| 4Center of Excellence in Cereal Molecular Breeding, University of Tabriz, Tabriz, Iran | ||
| چکیده | ||
| Lemon balm (Melissa officinalis L.) is popular as an alternative to standard medicine for a variety of problems and its essential oil is widely used in pharmacology. The isolation of good quality DNA is the pre-requisite for molecular research. Due to high amounts of polysaccharides, polyphenols and various secondary metabolites, DNA extraction from lemon balm is problematic. In this study, three different CTAB based methods by some modification were compared for the isolation of high‑quality genomic DNA from lemon balm. The DNA from the leaves of five lemon balm ecotypes was extracted using CTAB methods A (with PVP and phenol), B (PVP, Proteinase K and Na acetate) and C (PVP, Tri sodium citrate and NaCl). The quality of DNA samples was determined by physical appearance, agarose gel electrophoresis, spectrophometer, PCR amplification and restriction. Based on the results of various DNA quality analysis, the CTAB method C was found to be the most efficient one. Method C was found to be the most efficient DNA extraction method for lemon balm providing high DNA yields with better quality, in short time with less cost. It could affordable for DNA extraction from medicinal plants with similar secondary metabolites content. | ||
| کلیدواژهها | ||
| DNA extraction؛ Melissa officinalis؛ PCR amplification؛ Restriction digestion | ||
| مراجع | ||
|
1. Adinee J, Piri KH, Karami O. Essential Oil Component in Flower of Lemon Balm (Melissa officinalis L.). Am J Biochem Biotechnol. 2008;4:277-8.
2. Kennedy DO, Little W, Scholey AB. Attenuation of Laboratory-Induced Stress in Humans After Acute Administration of Melissa officinalis (Lemon Balm). Psychosom Med. 2004; 66:607-13.
3. Sorensen JM. Melissa officinalis, essential oil, authenticity, production and pharmalogical activity. Int J Aromatherapy. 2000;10:7-15.
4. Allahverdiyev A, Duran N, Ozguven M, Koltas S, Antiviral activity of the volatile oils of Melissa officinalis L. against Herpes simplex virus type-2. Phytomedicine, 2004;11:657-61.
5. Vines G. Herbal harvests with a future: towards sustainable sources for medicinal plants. Plant life Int. 2004.
6. Shi-Lin Chen SL, Yu H, Luo HM, Wu Q, Li CF, Steinmetz A. Conservation and sustainable use of medicinal plants: problems, progress, and prospects. Chin Med. 2016;11:37 doi.org/10.1186/s13020-016-0108-7
7. Padulosi S, Leaman D, Quek P. Challenges and opportunities in enhancing the conservation and use of medicinal and aromatic plants. J Herb Spices Med Plants. 2002;4:243-267.
8. Tang SQ, Bin XY, Peng YT, Zhou JY, Wang L, Zhong Y. Assessment of genetic diversity in cultivars and wild accessions of luohanguo (siraitiagros venorii [swingle] A. M. Lu et Z. Y. Zhang), a species with edible and medicinal sweet fruits endemic to southern china, using RAPD and AFLP markers. Genet Resour Evol. 2007;54:1053-61.
9. Barbosa TC, Sibov ST, Telles MP, Soares TN. Genetic characterization of natural populations of the medicinal plant Palicoureacoriacea (Rubiaceae) with molecular markers. Genet Mol Res. 2010; 9:695-704.
10. Mohammadi SA, Shokrpour M, Moghaddam M, Javanshir A. AFLP-based molecular characterization and population structure analysis of Silybum marianum L. Plant Genet Resour-C. 2011;9:445-453.
11. Pinheiro LR, Rabbani ARC, da Silva AVC, da Silva Lédo A, Pereira KLC, Diniz LEC. Genetic diversity and population structure in the Brazilian Cattleyalabiata (Orchidaceae) using RAPD and ISSR markers. Plant SystEvol. 2011;doi.org/10.1007/s00606-012-0682.
12. Asadkhani Mamaghani R, Mohammadi SA, Aharizad S. Transferability of barley retrotransposon primers to analyze genetic structure in Iranian Hypericum perforatum L. populations. Turk J Bot. 2015; 39:664-672.
13. Bao W, Wuyun T, Li T, Liu M, Jiang Z, Zhu X, Du H, Bai Y. Genetic diversity and population structure of Prunusmira (Koehne) from the Tibet plateau in China and recommended conservation strategies. PLOS ONE 2017;doi.org/10.1371/journal.pone.0188685
14. Gupta A, Pandey-Rai S, Srivastava S, Naithani SC, Prasad M, Kumar S. Construction of genetic linkage map of the medicinal and ornamental plant Catharanthusroseus. J Genet. 2007;86:259-68.
15. Doyle JJ, Doyle JL. A rapid total DNA preparation procedure for fresh plant tissue. Focus. 1990;12:13-5.
16. Sharma KK, Lavanya M, Anjaiah V. A method for isolation and purification of peanut genomic DNA suitable for analytical applications. Plant Mol Biol Rep. 2000;8:393.
17. Pirttilä AM, Hirsikorpi M, Kämäräinen T, Jaakola L, Hohtola A.DNA isolation methods for medicinal and aromatic plants. Plant Mol Biol Rep. 2001;19:273.
18. Shepherd M, Cross M, Stokoe RL, Scott LJ, Jones ME. High-throughput DNA extraction from forest trees. Plant Mol Biol Rep. 2002;20:425.
19. Mogg RJ, Bond JM. A cheap, reliable and rapid method of extracting high-quality DNA from plants. Mol Ecol Note. 2003;3:666-8.
20. Haymes KM, Ibrahim IA, Mischke S, Scott DL, Saunders JA. Rapid isolation of DNA from chocolate and date palm tree crops. J Agric Food Chem. 2004;52: 456-62.
21. Weishing K, Nybom H, Wolff K, Meyer W. DNA isolation and purification. In: DNA fingerprinting in plants and fungi, Florida: CRC Press; 1995. P. 44-59.
22. Sharma AD, Gill PK, Singh P. DNA isolation from dry and fresh samples of polysaccharide-rich plants. Plant Mol Bio Rep. 2002;20:415a-f.
23. Bousquet J, Simon L, Lalonde M. DNA amplification from vegetative and sexual tissues of trees using polymerase chain reaction. Can J Forest Res. 1990;20:254-7.
24. Khanuja SPS, Shasany AK, Darokar MP, Kumar S. Rapid Isolation of DNA from Dry and Fresh Samples of Plants Producing Large Amounts of Secondary Metabolites and Essential Oils. Plant Mol Biol Rep. 1999:17:1-7.
25. Porebski S, Bailey LG, Baum BR. Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant MolBiol Rep. 1997;15:8-15.
26. Guillemaut P, Maréchal-Drouard L. Isolation of plant DNA: A fast, inexpensive, and reliable method. Plant molbiol rep.1992;10:60-5.
27. Scott KD, Playford J. DNA extraction technique for PCR in rain forest plant species. Biotechniques. 1996;20:974-9.
28. Demeke T, Adams RP. The effect of plant polysaccharides and buffer additives of PCR. Biotechniques, 1992;12:332-4. 29. Pandy RN, Adams RP, Flournoy LE. Inhibition of random amplified polymorphic DNAs (RAPDs) by plant polysaccharides. Plant MolBiol Rep. 1996;14:17-22.
30. Fang G, Hammer S, and Groumet RA. Quick and inexpensive method for removing polysaccharides from plant genomic DNA. Bio feedback. 1992;13:52-4.
31. Varma A, Padh H, Shrivastava N. Plant genomic DNA isolation: an art or a science Biotech J. 2007;2:386-392.
32. Moyo M, Amoo SO, Bairu MW, Finnie JF, Van Staden J. Optimising DNA isolation for medicinal plants. S Afr J Bot. 2008;74:771-5.
33. Pateraki I, Kanellis AK. Isolation of high-quality nucleic acids from Cistus creticus sp creticus and other medicinal plants. Anal Biochem. 2004;328:90-2.
34. Saghai Maroof MA, Soliman K, Torgensen RA and Allard RW. Ribosomal DNA spacer-length polymorphism in barley: Mendelian inheritance, choromosomal location, and population dynamics. Proc. Natl Acad Sci USA. 1984;81:8014-18.
35. Lodhi MA, Guang-Ning Y, Weeden FN, Reisch BI. A simple and effiient method for DNA extraction from grapevine cultivars and Vitis species. Plant Mol Biol Rep. 1994;12:6-13.
36. Ghaffariyan S, Mohammadi SA, Aharizad S. DNA isolation protocol for the medicinal plant lemon balm (Melissa offiinalis, Lamiaceae). Genet Mol Res. 2012;11:1049-57.
37. Dellaporta SL, Wood J, Hicks, JB. A plant DNA mini preparation: Version II. Plant Mol Biol Rep. 1983;1:19-21.
38. Joun ME. An efficient method for isolation of RNA and DNA from plants containing polyphenolics. Nucleic Acids Res. 1992;20:2381. | ||
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